Journal: Antimicrobial Agents and Chemotherapy
Article Title: A novel pan-fungal screening platform for antifungal drug discovery: proof of principle study
doi: 10.1128/aac.01328-24
Figure Lengend Snippet: Forty-eighty-hour time-course kinetic growth curves and media comparison for mold species. Horizontal dotted lines on the y-axis represent an OD threshold of 0.2. Black lines represent RPMI 2% G-MOPS, and purple lines represent fRPMI. Vertical dotted lines on the x-axis represent the approximate optimal measurement times ( T a ) for each media. Individual species are represented in A–F as 2A A . fumigatus A1160 + , 2B Aspergillus flavus CBS 128202, 2C Aspergillus niger 1D Aspergillus terreus , 1E L. corymbifera NCPF 2710, and 1F Rhizopus microsporus FP469-12 NCPF 3207. Data points represent the mean, and error bars represent ±SD of 3–4 biological replicates.
Article Snippet: For our proof-of-principle study, 500 chemical fragments from the Maybridge Ro3 Fragment library were screened at concentrations of 0.1 or 1 mM against five fungal pathogens: Aspergillus fumigatus , Candida albicans , Candida auris , Cryptococcus neoformans , and Nakaseomyces glabratus .
Techniques: Comparison