Journal: Molecular neurobiology

Article Title: Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid-Sensing of Mouse Olfactory Sensory Neurons

doi: 10.1007/s12035-020-01943-0

Figure Lengend Snippet: Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, the MOE on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, ASIC activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.

Article Snippet: To examine whether other ASIC subtypes are expressed in the mouse MOE, we tested an anti-ASIC2 antibody (Catalog# ASC-012, Alomone Labs).

Techniques: Activation Assay, Transmission Assay

Journal: Bioscience Reports

Article Title: ASIC1a activation induces calcium-dependent apoptosis of BMSCs under conditions that mimic the acidic microenvironment of the degenerated intervertebral disc

doi: 10.1042/BSR20192708

Figure Lengend Snippet: Gene expression was measured using real-time RT-PCR, and mRNA levels of ASIC1 in the rat cortex (positive control) were normalized to 1 ( A ). Western blot analysis showed the expression of ASIC1a in BMSCs was lower than that in cortex ( B,C ). ASIC1a was found in the cytoplasm and cytomembrane of BMSCs ( D ). Negative controls with nonspecific rabbit IgG gave no signal. ** P <0.01, n =5.

Article Snippet: The membranes were blocked with 5% BSA in Tris-buffered saline containing 0.1% Tween 20 (Sigma, U.S.A.) for 1 h. Then, the membranes were incubated with rabbit anti-ASIC1a (1:200, Alomone Labs, Israel), anti-pBAD (1:1000), anti-tBAD (1:2000), anti-cytochrome C (Cyt C, 1:1000), anti-cleaved caspase-9 (1:1000), anti-cleaved caspase-8 (1:1000), anti-cleaved caspase-3 (1:1000), and anti-α-tubulin (1:2500) at 4°C with gentle shaking overnight.

Techniques: Expressing, Quantitative RT-PCR, Positive Control, Western Blot

Journal: Bioscience Reports

Article Title: ASIC1a activation induces calcium-dependent apoptosis of BMSCs under conditions that mimic the acidic microenvironment of the degenerated intervertebral disc

doi: 10.1042/BSR20192708

Figure Lengend Snippet: Ca 2+ -imaging assay showed a significant increase in [Ca 2+ ]i in response to pH 6.0 solution containing Ca 2+ ( A ). MTT assay showed that ASIC1a blockade with PcTX1 and Ca 2+ -free extracellular solution both increased BMSCs survival in acid ( B ). ** P <0.01, n =5.

Article Snippet: The membranes were blocked with 5% BSA in Tris-buffered saline containing 0.1% Tween 20 (Sigma, U.S.A.) for 1 h. Then, the membranes were incubated with rabbit anti-ASIC1a (1:200, Alomone Labs, Israel), anti-pBAD (1:1000), anti-tBAD (1:2000), anti-cytochrome C (Cyt C, 1:1000), anti-cleaved caspase-9 (1:1000), anti-cleaved caspase-8 (1:1000), anti-cleaved caspase-3 (1:1000), and anti-α-tubulin (1:2500) at 4°C with gentle shaking overnight.

Techniques: Imaging, MTT Assay