anti asic 3  (Alomone Labs)


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    Alomone Labs anti asic 3
    Anti Asic 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti asic 3  (Alomone Labs)


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    Alomone Labs anti asic 3
    Anti Asic 3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    α pan akt  (Alomone Labs)


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    Alomone Labs α pan akt
    α Pan Akt, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    asic inhibitors  (Alomone Labs)


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    Alomone Labs asic inhibitors
    Asic Inhibitors, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    asic inhibitors  (Alomone Labs)


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    Alomone Labs asic inhibitors
    Asic Inhibitors, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    asic subtypes  (Alomone Labs)


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    Alomone Labs asic subtypes
    Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, <t>the</t> <t>MOE</t> on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, <t>ASIC</t> activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.
    Asic Subtypes, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic subtypes/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic subtypes - by Bioz Stars, 2023-01
    93/100 stars

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    1) Product Images from "Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid-Sensing of Mouse Olfactory Sensory Neurons"

    Article Title: Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid-Sensing of Mouse Olfactory Sensory Neurons

    Journal: Molecular neurobiology

    doi: 10.1007/s12035-020-01943-0

    Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, the MOE on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, ASIC activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.
    Figure Legend Snippet: Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, the MOE on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, ASIC activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.

    Techniques Used: Activation Assay, Transmission Assay

    asic subtypes  (Alomone Labs)


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    Alomone Labs asic subtypes
    Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, <t>the</t> <t>MOE</t> on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, <t>ASIC</t> activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.
    Asic Subtypes, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic subtypes/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
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    Images

    1) Product Images from "Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid-Sensing of Mouse Olfactory Sensory Neurons"

    Article Title: Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid-Sensing of Mouse Olfactory Sensory Neurons

    Journal: Molecular neurobiology

    doi: 10.1007/s12035-020-01943-0

    Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, the MOE on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, ASIC activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.
    Figure Legend Snippet: Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, the MOE on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, ASIC activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.

    Techniques Used: Activation Assay, Transmission Assay

    asic sodium channels  (Alomone Labs)


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    Alomone Labs asic sodium channels
    Asic Sodium Channels, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    asic sodium channels  (Alomone Labs)


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    Alomone Labs asic sodium channels
    Asic Sodium Channels, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti asic1a  (Alomone Labs)


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    Alomone Labs rabbit anti asic1a
    Gene expression was measured using real-time RT-PCR, and mRNA levels of ASIC1 in the rat cortex (positive control) were normalized to 1 ( A ). Western blot analysis showed the expression of <t>ASIC1a</t> in BMSCs was lower than that in cortex ( B,C ). ASIC1a was found in the cytoplasm and cytomembrane of BMSCs ( D ). Negative controls with nonspecific rabbit IgG gave no signal. ** P <0.01, n =5.
    Rabbit Anti Asic1a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "ASIC1a activation induces calcium-dependent apoptosis of BMSCs under conditions that mimic the acidic microenvironment of the degenerated intervertebral disc"

    Article Title: ASIC1a activation induces calcium-dependent apoptosis of BMSCs under conditions that mimic the acidic microenvironment of the degenerated intervertebral disc

    Journal: Bioscience Reports

    doi: 10.1042/BSR20192708

    Gene expression was measured using real-time RT-PCR, and mRNA levels of ASIC1 in the rat cortex (positive control) were normalized to 1 ( A ). Western blot analysis showed the expression of ASIC1a in BMSCs was lower than that in cortex ( B,C ). ASIC1a was found in the cytoplasm and cytomembrane of BMSCs ( D ). Negative controls with nonspecific rabbit IgG gave no signal. ** P <0.01, n =5.
    Figure Legend Snippet: Gene expression was measured using real-time RT-PCR, and mRNA levels of ASIC1 in the rat cortex (positive control) were normalized to 1 ( A ). Western blot analysis showed the expression of ASIC1a in BMSCs was lower than that in cortex ( B,C ). ASIC1a was found in the cytoplasm and cytomembrane of BMSCs ( D ). Negative controls with nonspecific rabbit IgG gave no signal. ** P <0.01, n =5.

    Techniques Used: Expressing, Quantitative RT-PCR, Positive Control, Western Blot

    Ca 2+ -imaging assay showed a significant increase in [Ca 2+ ]i in response to pH 6.0 solution containing Ca 2+ ( A ). MTT assay showed that ASIC1a blockade with PcTX1 and Ca 2+ -free extracellular solution both increased BMSCs survival in acid ( B ). ** P <0.01, n =5.
    Figure Legend Snippet: Ca 2+ -imaging assay showed a significant increase in [Ca 2+ ]i in response to pH 6.0 solution containing Ca 2+ ( A ). MTT assay showed that ASIC1a blockade with PcTX1 and Ca 2+ -free extracellular solution both increased BMSCs survival in acid ( B ). ** P <0.01, n =5.

    Techniques Used: Imaging, MTT Assay

    asic receptors  (Alomone Labs)


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    Alomone Labs asic receptors
    Asic Receptors, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs asic subtypes
    Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, <t>the</t> <t>MOE</t> on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, <t>ASIC</t> activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.
    Asic Subtypes, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic subtypes/product/Alomone Labs
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    Alomone Labs asic sodium channels
    Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, <t>the</t> <t>MOE</t> on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, <t>ASIC</t> activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.
    Asic Sodium Channels, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs rabbit anti asic1a
    Gene expression was measured using real-time RT-PCR, and mRNA levels of ASIC1 in the rat cortex (positive control) were normalized to 1 ( A ). Western blot analysis showed the expression of <t>ASIC1a</t> in BMSCs was lower than that in cortex ( B,C ). ASIC1a was found in the cytoplasm and cytomembrane of BMSCs ( D ). Negative controls with nonspecific rabbit IgG gave no signal. ** P <0.01, n =5.
    Rabbit Anti Asic1a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs asic receptors
    Gene expression was measured using real-time RT-PCR, and mRNA levels of ASIC1 in the rat cortex (positive control) were normalized to 1 ( A ). Western blot analysis showed the expression of <t>ASIC1a</t> in BMSCs was lower than that in cortex ( B,C ). ASIC1a was found in the cytoplasm and cytomembrane of BMSCs ( D ). Negative controls with nonspecific rabbit IgG gave no signal. ** P <0.01, n =5.
    Asic Receptors, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, the MOE on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, ASIC activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.

    Journal: Molecular neurobiology

    Article Title: Acid-Sensing Ion Channels Contribute to Type III Adenylyl Cyclase-Independent Acid-Sensing of Mouse Olfactory Sensory Neurons

    doi: 10.1007/s12035-020-01943-0

    Figure Lengend Snippet: Top, a schematic of acidic volatiles inhaled into the mouse nasal cavity. Bottom, the MOE on the top enlarged to the bottom with single OSN shown to the right. Acidic volatiles (e.g., acetic acid) are dissolved in mucosa and dissociated into protons (H+) and base (acetate). Based on our data, we propose that OSNs in the MOE can be stimulated by protons and base separately. While the base moiety initiates the conventional AC3-medaited cAMP pathway in olfactory cilia, protons directly activate ASICs expressed in the knob, dendrite, and soma, promoting the depolarization of OSNs. As ASICs are more widely expressed than individual subclass of receptor-specific OSNs, ASIC activation may unselectively depolarize different subclasses of OSNs, interfering with the anatomical logic of neural information transmission for specific odorants.

    Article Snippet: To examine whether other ASIC subtypes are expressed in the mouse MOE, we tested an anti-ASIC2 antibody (Catalog# ASC-012, Alomone Labs).

    Techniques: Activation Assay, Transmission Assay

    Gene expression was measured using real-time RT-PCR, and mRNA levels of ASIC1 in the rat cortex (positive control) were normalized to 1 ( A ). Western blot analysis showed the expression of ASIC1a in BMSCs was lower than that in cortex ( B,C ). ASIC1a was found in the cytoplasm and cytomembrane of BMSCs ( D ). Negative controls with nonspecific rabbit IgG gave no signal. ** P <0.01, n =5.

    Journal: Bioscience Reports

    Article Title: ASIC1a activation induces calcium-dependent apoptosis of BMSCs under conditions that mimic the acidic microenvironment of the degenerated intervertebral disc

    doi: 10.1042/BSR20192708

    Figure Lengend Snippet: Gene expression was measured using real-time RT-PCR, and mRNA levels of ASIC1 in the rat cortex (positive control) were normalized to 1 ( A ). Western blot analysis showed the expression of ASIC1a in BMSCs was lower than that in cortex ( B,C ). ASIC1a was found in the cytoplasm and cytomembrane of BMSCs ( D ). Negative controls with nonspecific rabbit IgG gave no signal. ** P <0.01, n =5.

    Article Snippet: The membranes were blocked with 5% BSA in Tris-buffered saline containing 0.1% Tween 20 (Sigma, U.S.A.) for 1 h. Then, the membranes were incubated with rabbit anti-ASIC1a (1:200, Alomone Labs, Israel), anti-pBAD (1:1000), anti-tBAD (1:2000), anti-cytochrome C (Cyt C, 1:1000), anti-cleaved caspase-9 (1:1000), anti-cleaved caspase-8 (1:1000), anti-cleaved caspase-3 (1:1000), and anti-α-tubulin (1:2500) at 4°C with gentle shaking overnight.

    Techniques: Expressing, Quantitative RT-PCR, Positive Control, Western Blot

    Ca 2+ -imaging assay showed a significant increase in [Ca 2+ ]i in response to pH 6.0 solution containing Ca 2+ ( A ). MTT assay showed that ASIC1a blockade with PcTX1 and Ca 2+ -free extracellular solution both increased BMSCs survival in acid ( B ). ** P <0.01, n =5.

    Journal: Bioscience Reports

    Article Title: ASIC1a activation induces calcium-dependent apoptosis of BMSCs under conditions that mimic the acidic microenvironment of the degenerated intervertebral disc

    doi: 10.1042/BSR20192708

    Figure Lengend Snippet: Ca 2+ -imaging assay showed a significant increase in [Ca 2+ ]i in response to pH 6.0 solution containing Ca 2+ ( A ). MTT assay showed that ASIC1a blockade with PcTX1 and Ca 2+ -free extracellular solution both increased BMSCs survival in acid ( B ). ** P <0.01, n =5.

    Article Snippet: The membranes were blocked with 5% BSA in Tris-buffered saline containing 0.1% Tween 20 (Sigma, U.S.A.) for 1 h. Then, the membranes were incubated with rabbit anti-ASIC1a (1:200, Alomone Labs, Israel), anti-pBAD (1:1000), anti-tBAD (1:2000), anti-cytochrome C (Cyt C, 1:1000), anti-cleaved caspase-9 (1:1000), anti-cleaved caspase-8 (1:1000), anti-cleaved caspase-3 (1:1000), and anti-α-tubulin (1:2500) at 4°C with gentle shaking overnight.

    Techniques: Imaging, MTT Assay