asic1a specific inhibitor psalmotoxin 1 pctx1  (ApexBio)

 
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    ApexBio asic1a specific inhibitor psalmotoxin 1 pctx1
    Exposure to an acidic medium made the expression of <t>ASIC1a</t> in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Asic1a Specific Inhibitor Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic1a specific inhibitor psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic1a specific inhibitor psalmotoxin 1 pctx1 - by Bioz Stars, 2023-12
    86/100 stars

    Images

    1) Product Images from "ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction"

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    Journal: Physiological Research

    doi: 10.33549/physiolres.934950

    Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Figure Legend Snippet: Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).

    Techniques Used: Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).
    Figure Legend Snippet: ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).
    Figure Legend Snippet: ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.
    Figure Legend Snippet: Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    asic1a specific inhibitor psalmotoxin 1 pctx1  (ApexBio)

     
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    Structured Review

    ApexBio asic1a specific inhibitor psalmotoxin 1 pctx1
    Exposure to an acidic medium made the expression of <t>ASIC1a</t> in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Asic1a Specific Inhibitor Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic1a specific inhibitor psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic1a specific inhibitor psalmotoxin 1 pctx1 - by Bioz Stars, 2023-12
    86/100 stars

    Images

    1) Product Images from "ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction"

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    Journal: Physiological Research

    doi: 10.33549/physiolres.934950

    Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Figure Legend Snippet: Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).

    Techniques Used: Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).
    Figure Legend Snippet: ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).
    Figure Legend Snippet: ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.
    Figure Legend Snippet: Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.

    Techniques Used: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

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    • asic1a specific inhibitor psalmotoxin 1 pctx1  (ApexBio)
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    ApexBio asic1a specific inhibitor psalmotoxin 1 pctx1
    Exposure to an acidic medium made the expression of <t>ASIC1a</t> in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).
    Asic1a Specific Inhibitor Psalmotoxin 1 Pctx1, supplied by ApexBio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic1a specific inhibitor psalmotoxin 1 pctx1/product/ApexBio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asic1a specific inhibitor psalmotoxin 1 pctx1 - by Bioz Stars, 2023-12
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).

    Journal: Physiological Research

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    doi: 10.33549/physiolres.934950

    Figure Lengend Snippet: Exposure to an acidic medium made the expression of ASIC1a in PSCs increase obviously. Immunofluorescence staining of ASIC1a (red) and cell nuclei (blue) (A). RT-PCR (B) and Western blotting result (C) of ASIC1a expression in control group (pH 7.4) and acidic medium group (pH 6.5). * P<0.05 (n=3).

    Article Snippet: ASIC1a specific inhibitor Psalmotoxin-1 (PcTx1) was provided by ApexBio Technology.

    Techniques: Expressing, Immunofluorescence, Staining, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Journal: Physiological Research

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    doi: 10.33549/physiolres.934950

    Figure Lengend Snippet: ASIC1a involved the acid-induced activation of PSCs. The expression of α-SMA and collagen I in PSCs at different conditions was measured at mRNA level by RT-PCR (A–B) or at protein level by Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Article Snippet: ASIC1a specific inhibitor Psalmotoxin-1 (PcTx1) was provided by ApexBio Technology.

    Techniques: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Journal: Physiological Research

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    doi: 10.33549/physiolres.934950

    Figure Lengend Snippet: ASIC1a mediated the acid-induced autophagy of PSCs. After the corresponding treatment, autophagy-associated important molecules, Beclin 1, LC3B and p62 expression in PSCs were detected by RT-PCR (A–C) and Western blotting (D). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results. I (Control), II (pH 6.5), III (pH 6.5 + PcTx1), IV (pH 6.5 + ASIC1a siRNA), V (pH 6.5 + NC-siRNA).

    Article Snippet: ASIC1a specific inhibitor Psalmotoxin-1 (PcTx1) was provided by ApexBio Technology.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.

    Journal: Physiological Research

    Article Title: ASIC1a Involves the Acid-Mediated Activation of Pancreatic Stellate Cells Associated With Autophagy Induction

    doi: 10.33549/physiolres.934950

    Figure Lengend Snippet: Involvement of autophagy in ASIC1a mediated acid-induced PSCs activation. After treatment with autophagy inhibitor alone or together with ASIC1a knockdown, the expression of α-SMA and collagen I in PSCs was detected by RT-PCR (A–B) and Western blotting (C). * P<0.05 (n=3). Repeated Western blotting experiments showed similar results.

    Article Snippet: ASIC1a specific inhibitor Psalmotoxin-1 (PcTx1) was provided by ApexBio Technology.

    Techniques: Activation Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot