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Santa Cruz Biotechnology asic1
Acid-sensing ion channel 1 <t>(ASIC1),</t> TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
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Images

1) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

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2) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

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3) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

4) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

5) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

6) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

7) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

8) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

9) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

10) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

11) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

12) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

13) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

14) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

15) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

16) Product Images from "ASIC1 and ASIC3 contribute to acidity-induced EMT of pancreatic cancer through activating Ca2+/RhoA pathway"

Article Title: ASIC1 and ASIC3 contribute to acidity-induced EMT of pancreatic cancer through activating Ca2+/RhoA pathway

Journal: Cell Death & Disease

doi: 10.1038/cddis.2017.189

The acidity-induced elevation of [Ca 2+ ]i is suppressed by inhibition of ASIC1 and ASIC3. ( a ) Calcium imaging detected dynamic change in [Ca 2+ ]i when PANC-1 and BxPC-3 cells were treated with different acidic pH medium at 90 s. Pictures showed [Ca 2+ ]i indicated by Fura-2/AM at indicated time. ( b and c ) After inhibition of ASIC1 and ASIC3 by siRNA or amiloride, calcium imaging detected dynamic change in [Ca 2+ ]i of PANC-1 and BxPC-3 cells in acidic medium (pH 6.4). Pictures showed intracellular Ca 2+ concentration indicated by Fura-2/AM at indicated time. Experiments were performed three times
Figure Legend Snippet: The acidity-induced elevation of [Ca 2+ ]i is suppressed by inhibition of ASIC1 and ASIC3. ( a ) Calcium imaging detected dynamic change in [Ca 2+ ]i when PANC-1 and BxPC-3 cells were treated with different acidic pH medium at 90 s. Pictures showed [Ca 2+ ]i indicated by Fura-2/AM at indicated time. ( b and c ) After inhibition of ASIC1 and ASIC3 by siRNA or amiloride, calcium imaging detected dynamic change in [Ca 2+ ]i of PANC-1 and BxPC-3 cells in acidic medium (pH 6.4). Pictures showed intracellular Ca 2+ concentration indicated by Fura-2/AM at indicated time. Experiments were performed three times

Techniques Used: Inhibition, Imaging, Concentration Assay

ASIC1/ASIC3-[Ca 2+ ]i signaling pathway upregulates RhoA activity of pancreatic cancer cells in acidity condition. ( a ) RhoA activity (GTP-bound RhoA) was detected in PANC-1 and BxPC-3 treated in pH 6.4 or pH 7.4 medium as indicated for 48 h. ( b ) After inhibition of ASIC1 and ASIC3 by siRNA or amiloride, the RhoA activity was detected in PANC-1 and BxPC-3 cells, which were incubated acidity medium (pH 6.4) for 48 h. ( c ) With pretreatment of BAPTA-AM or DMSO for 1 h, RhoA activity was detected in PANC-1 and BxPC-3 cells after cells were incubated with acidic medium (pH 6.4) for 48 h. PANC-1 and BxPC-3 were transfected with negative control (NC) or siRNA of RhoA (siRhoA) and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. ( d ) The invasive ability was evaluated by Transwell assay and the histogram showed the percentage of invasion cells per field when compared with negative control cultured in pH 6.4 medium. ( e ) The migration ability was measured by wound-healing assay. ( f ) The representative pictures of cellular morphology. ( g ) The mRNA of N-cadherin, E-cadherin, Vimentin, ZEB1, Snail was measured by qRT-PCR. ( h ) The protein of N-cadherin, E-cadherin, Vimentin, ZEB1, Snail was measured by western blot. Values were normalized against negative control in pH 6.4 medium. Experiments were performed three times in triplicate and are presented as means±S.D. (* P
Figure Legend Snippet: ASIC1/ASIC3-[Ca 2+ ]i signaling pathway upregulates RhoA activity of pancreatic cancer cells in acidity condition. ( a ) RhoA activity (GTP-bound RhoA) was detected in PANC-1 and BxPC-3 treated in pH 6.4 or pH 7.4 medium as indicated for 48 h. ( b ) After inhibition of ASIC1 and ASIC3 by siRNA or amiloride, the RhoA activity was detected in PANC-1 and BxPC-3 cells, which were incubated acidity medium (pH 6.4) for 48 h. ( c ) With pretreatment of BAPTA-AM or DMSO for 1 h, RhoA activity was detected in PANC-1 and BxPC-3 cells after cells were incubated with acidic medium (pH 6.4) for 48 h. PANC-1 and BxPC-3 were transfected with negative control (NC) or siRNA of RhoA (siRhoA) and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. ( d ) The invasive ability was evaluated by Transwell assay and the histogram showed the percentage of invasion cells per field when compared with negative control cultured in pH 6.4 medium. ( e ) The migration ability was measured by wound-healing assay. ( f ) The representative pictures of cellular morphology. ( g ) The mRNA of N-cadherin, E-cadherin, Vimentin, ZEB1, Snail was measured by qRT-PCR. ( h ) The protein of N-cadherin, E-cadherin, Vimentin, ZEB1, Snail was measured by western blot. Values were normalized against negative control in pH 6.4 medium. Experiments were performed three times in triplicate and are presented as means±S.D. (* P

Techniques Used: Activity Assay, Inhibition, Incubation, Transfection, Negative Control, Cell Culture, Transwell Assay, Migration, Wound Healing Assay, Quantitative RT-PCR, Western Blot

Inhibition of ASIC1 and ASIC3 suppresses acidity-promoted invasion and migration of pancreatic cancer cells. PANC-1 and BxPC-3 were transfected with negative control (NC) or siRNA of ASIC1 and ASIC3 (siASIC1 and siASIC3) and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. ( a ) The invasive ability was evaluated by Transwell assay. The histogram showed the percentage of invasion cells per field when compared with negative control cultured in pH 6.4 medium. ( b ) The migration ability was measured by wound-healing assay. ( c ) The cell viability was measured by MTT assay. PANC-1 and BxPC-3 were treated with amiloride and DMSO, and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. ( d ) The invasive ability was evaluated by Transwell assay. The histogram showed the percentage of invasion cells per field when compared with negative control cultured in pH 6.4 medium. ( e ) The migration ability was measured by wound-healing assay. Experiments were performed three times in triplicate and were presented as means±S.D. (* P
Figure Legend Snippet: Inhibition of ASIC1 and ASIC3 suppresses acidity-promoted invasion and migration of pancreatic cancer cells. PANC-1 and BxPC-3 were transfected with negative control (NC) or siRNA of ASIC1 and ASIC3 (siASIC1 and siASIC3) and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. ( a ) The invasive ability was evaluated by Transwell assay. The histogram showed the percentage of invasion cells per field when compared with negative control cultured in pH 6.4 medium. ( b ) The migration ability was measured by wound-healing assay. ( c ) The cell viability was measured by MTT assay. PANC-1 and BxPC-3 were treated with amiloride and DMSO, and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. ( d ) The invasive ability was evaluated by Transwell assay. The histogram showed the percentage of invasion cells per field when compared with negative control cultured in pH 6.4 medium. ( e ) The migration ability was measured by wound-healing assay. Experiments were performed three times in triplicate and were presented as means±S.D. (* P

Techniques Used: Inhibition, Migration, Transfection, Negative Control, Cell Culture, Transwell Assay, Wound Healing Assay, MTT Assay

ASIC1 and ASIC3 are overexpressed and correlated to EMT marker in pancreatic cancer. ( a ) The mRNA expression of ASIC1 and ASIC3 in pancreatic cancer (PC) tissues when compared with matched adjacent noncancerous pancreatic (NP) tissues. Values were normalized against negative control in pH 6.4. Experiments were performed three times in triplicate and are presented as means±S.D. (* P
Figure Legend Snippet: ASIC1 and ASIC3 are overexpressed and correlated to EMT marker in pancreatic cancer. ( a ) The mRNA expression of ASIC1 and ASIC3 in pancreatic cancer (PC) tissues when compared with matched adjacent noncancerous pancreatic (NP) tissues. Values were normalized against negative control in pH 6.4. Experiments were performed three times in triplicate and are presented as means±S.D. (* P

Techniques Used: Marker, Expressing, Negative Control

Knockdown of ASIC1 and ASIC3 suppresses acidity-induced EMT of pancreatic cancer cells. ( a and b ) PANC-1 and BxPC-3 were transfected with negative control (NC) or siRNA of ASIC1 and ASIC3 (siASIC1 and siASIC3) and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. The mRNA and protein expression of N-cadherin, E-cadherin, Vimentin, ZEB1, Snail were measured by qRT-PCR and western blot. ( c and d ) PANC-1 and BxPC-3 were treated with amiloride and DMSO, and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. The mRNA and protein expression of N-cadherin, E-cadherin, Vimentin, ZEB1, Snail were measured by qRT-PCR and western blot. Values were normalized against negative control in pH 6.4 medium. Experiments were performed three times in triplicate and are presented as means±S.D. (* P
Figure Legend Snippet: Knockdown of ASIC1 and ASIC3 suppresses acidity-induced EMT of pancreatic cancer cells. ( a and b ) PANC-1 and BxPC-3 were transfected with negative control (NC) or siRNA of ASIC1 and ASIC3 (siASIC1 and siASIC3) and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. The mRNA and protein expression of N-cadherin, E-cadherin, Vimentin, ZEB1, Snail were measured by qRT-PCR and western blot. ( c and d ) PANC-1 and BxPC-3 were treated with amiloride and DMSO, and cultured in pH 7.4 or pH 6.4 medium as indicated for 48 h. The mRNA and protein expression of N-cadherin, E-cadherin, Vimentin, ZEB1, Snail were measured by qRT-PCR and western blot. Values were normalized against negative control in pH 6.4 medium. Experiments were performed three times in triplicate and are presented as means±S.D. (* P

Techniques Used: Transfection, Negative Control, Cell Culture, Expressing, Quantitative RT-PCR, Western Blot

ASIC1 and ASIC3 are functionally expressed in pancreatic cancer. ( a and b ) The mRNA and protein expression of ASIC1 and ASIC3 in four pancreatic cancer cell lines (PANC-1, SW1990, BxPC-3 and AsPC-1) and normal pancreatic ductal cells (HPDE) were measured by RT-PCR, qRT-PCR and western blot. Values were normalized against HPDE from three experiments performed in triplicate and were presented as means±S.D. (* P
Figure Legend Snippet: ASIC1 and ASIC3 are functionally expressed in pancreatic cancer. ( a and b ) The mRNA and protein expression of ASIC1 and ASIC3 in four pancreatic cancer cell lines (PANC-1, SW1990, BxPC-3 and AsPC-1) and normal pancreatic ductal cells (HPDE) were measured by RT-PCR, qRT-PCR and western blot. Values were normalized against HPDE from three experiments performed in triplicate and were presented as means±S.D. (* P

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Knockdown of ASIC1 and ASIC3 inhibits metastasis of pancreatic cancer cells in vivo . ( a ) Photographs of nude mice and tumor showed subcutaneous xenograft of BxPC-3, which was transfected with lentivirus containing sequence of ASIC1 siRNA, ASIC3 siRNA or negative control (Lv-siASIC1, Lv-siASIC3 and Lv-NC). H E stain confirmed tumor formation. Graph represented tumor volumes at the indicated days during the experiment and tumor weight after mice were killed. ( b ) Pictures of liver and H E stain showed liver metastases of subcutaneous xenograft. The histogram showed average number of live metastases in each group. ( c ) H E stain showed lung metastases of subcutaneous xenograft. The histogram showed average number of lung metastases in each mouse. The data represent the mean±S.D. (* P
Figure Legend Snippet: Knockdown of ASIC1 and ASIC3 inhibits metastasis of pancreatic cancer cells in vivo . ( a ) Photographs of nude mice and tumor showed subcutaneous xenograft of BxPC-3, which was transfected with lentivirus containing sequence of ASIC1 siRNA, ASIC3 siRNA or negative control (Lv-siASIC1, Lv-siASIC3 and Lv-NC). H E stain confirmed tumor formation. Graph represented tumor volumes at the indicated days during the experiment and tumor weight after mice were killed. ( b ) Pictures of liver and H E stain showed liver metastases of subcutaneous xenograft. The histogram showed average number of live metastases in each group. ( c ) H E stain showed lung metastases of subcutaneous xenograft. The histogram showed average number of lung metastases in each mouse. The data represent the mean±S.D. (* P

Techniques Used: In Vivo, Mouse Assay, Transfection, Sequencing, Negative Control, Staining

17) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

18) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

19) Product Images from "Acid Sensing Ion Channel 1 in Lateral Hypothalamus Contributes to Breathing Control"

Article Title: Acid Sensing Ion Channel 1 in Lateral Hypothalamus Contributes to Breathing Control

Journal: PLoS ONE

doi: 10.1371/journal.pone.0039982

Distribution of ASIC1-ir and ASIC2a-ir neurons in the hypothalamus. A: ASIC1-ir neurons in the dorsal hypothalamus area (DA) (a, b, c) and in the lateral hypothalamus (LH) (d, e, f). B: ASIC2a-ir neurons in the DA (a, b, c) and the LH (d, e, f). C: c1∶1% BSA controls; c2: The peptides absorbed antibody control. D: Group data show the numbers of ASIC1- and ASIC2a-positive cells per visual field under microscope (×200) in the LH and DA. (*** P
Figure Legend Snippet: Distribution of ASIC1-ir and ASIC2a-ir neurons in the hypothalamus. A: ASIC1-ir neurons in the dorsal hypothalamus area (DA) (a, b, c) and in the lateral hypothalamus (LH) (d, e, f). B: ASIC2a-ir neurons in the DA (a, b, c) and the LH (d, e, f). C: c1∶1% BSA controls; c2: The peptides absorbed antibody control. D: Group data show the numbers of ASIC1- and ASIC2a-positive cells per visual field under microscope (×200) in the LH and DA. (*** P

Techniques Used: Microscopy

Co-expression of ASIC1 and OrexinA in the LH of adult SD rats. A representative confocal photomicrograph showing co-staining of orexinA and ASIC1 on neurons in the LH of an adult SD rat. A: OrexinA-ir neurons (green, FITC). B: ASIC1-ir neurons (red, cy3). C: Overlay of A and B.
Figure Legend Snippet: Co-expression of ASIC1 and OrexinA in the LH of adult SD rats. A representative confocal photomicrograph showing co-staining of orexinA and ASIC1 on neurons in the LH of an adult SD rat. A: OrexinA-ir neurons (green, FITC). B: ASIC1-ir neurons (red, cy3). C: Overlay of A and B.

Techniques Used: Expressing, Staining

20) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

21) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

22) Product Images from "TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats"

Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

Journal: Frontiers in Cellular Neuroscience

doi: 10.3389/fncel.2018.00285

Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
Figure Legend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

Techniques Used:

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    Santa Cruz Biotechnology goat anti asic1
    Effect of 30c and Let-7 on ASIC1a expression. CHO cells were transfected with ASIC1a-UTR together with the vector control, or expression constructs for miR-30c or let-7. Two days after transfection, cells were lysed and analyzed by Western blot using an <t>anti-ASIC1</t> and an anti-b-tubulin antibodies. Representative blots and quantification of ASIC1a: tubulin ratio was shown. Asterisk indicates significant (p
    Goat Anti Asic1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology asic1
    Acid-sensing ion channel 1 <t>(ASIC1),</t> TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
    Asic1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asic1/product/Santa Cruz Biotechnology
    Average 91 stars, based on 1 article reviews
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    asic1 - by Bioz Stars, 2022-10
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    Effect of 30c and Let-7 on ASIC1a expression. CHO cells were transfected with ASIC1a-UTR together with the vector control, or expression constructs for miR-30c or let-7. Two days after transfection, cells were lysed and analyzed by Western blot using an anti-ASIC1 and an anti-b-tubulin antibodies. Representative blots and quantification of ASIC1a: tubulin ratio was shown. Asterisk indicates significant (p

    Journal: International Journal of Physiology, Pathophysiology and Pharmacology

    Article Title: miR-149 reduces while let-7 elevates ASIC1a expression in vitro

    doi:

    Figure Lengend Snippet: Effect of 30c and Let-7 on ASIC1a expression. CHO cells were transfected with ASIC1a-UTR together with the vector control, or expression constructs for miR-30c or let-7. Two days after transfection, cells were lysed and analyzed by Western blot using an anti-ASIC1 and an anti-b-tubulin antibodies. Representative blots and quantification of ASIC1a: tubulin ratio was shown. Asterisk indicates significant (p

    Article Snippet: ASIC antibodies used: a rabbit anti-ASIC1 antibody [ ] and a goat anti-ASIC1 (Santa Cruz SC-13905).

    Techniques: Expressing, Transfection, Plasmid Preparation, Construct, Western Blot

    Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

    doi: 10.3389/fncel.2018.00285

    Figure Lengend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

    Article Snippet: After blocking, the slides were incubated with primary antibodies against TASK1 (Alomone Laboratory, Israel, 1:100), TASK3 (Alomone Laboratory, Israel, 1:100) and ASIC1 (Santa Cruz Biotechnology, Dallas, TX, USA, 1:100), Neurofilament-H (Abcam, Cambridge, MA, USA) which were diluted in 0.01 M PBS, overnight.

    Techniques: