Journal: OncoTargets and therapy

Article Title: Alteration of ASIC1 expression in clear cell renal cell carcinoma

doi: 10.2147/OTT.S86927

Figure Lengend Snippet: ASIC1 mRNA expression in matched tumor and normal kidney samples. Notes: The expression level of ASIC1 mRNA was sixfold higher in the normal tissue than that in the tumor tissue. *Represents P <0.05 and n=8. Abbreviation: ASIC1, acid-sensing ion channel 1.

Article Snippet: Membranes were then incubated with rabbit polyclonal anti-ASIC1 (ASC-014; Alomone Company; 1:200 dilutions) primary antibody in blocking solution at 4°C overnight.

Techniques: Expressing

Journal: OncoTargets and therapy

Article Title: Alteration of ASIC1 expression in clear cell renal cell carcinoma

doi: 10.2147/OTT.S86927

Figure Lengend Snippet: ( A ) ASIC1 protein expression in matched tumor and normal kidney samples. ( B ) Histogram shows the semiquantitative analyses of the gels from Western blots. Notes: The expression level of ASIC1 protein markedly decreased in RCC compared with corresponding adjacent normal tissue. *Denotes P <0.05 and n=8. T represents tumor sample; N represents matched normal sample. Abbreviations: ASIC1, acid-sensing ion channel 1; RCC, renal cell carcinoma; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: Membranes were then incubated with rabbit polyclonal anti-ASIC1 (ASC-014; Alomone Company; 1:200 dilutions) primary antibody in blocking solution at 4°C overnight.

Techniques: Expressing, Western Blot

Journal: OncoTargets and therapy

Article Title: Alteration of ASIC1 expression in clear cell renal cell carcinoma

doi: 10.2147/OTT.S86927

Figure Lengend Snippet: Pathological characteristics of patients and the ASIC1 staining intensity in tissue-microarray

Article Snippet: Membranes were then incubated with rabbit polyclonal anti-ASIC1 (ASC-014; Alomone Company; 1:200 dilutions) primary antibody in blocking solution at 4°C overnight.

Techniques: Staining

Journal: OncoTargets and therapy

Article Title: Alteration of ASIC1 expression in clear cell renal cell carcinoma

doi: 10.2147/OTT.S86927

Figure Lengend Snippet: ASIC1 immunohistochemistry. Notes: (A) Normal renal tissue with prominent ASIC1 expression. ( B – D ) ASIC1 expression in CCRCC with high- ( B ), intermediate- ( C ), and low- ( D ) staining density, respectively. Abbreviations: ASIC1, acid-sensing ion channel 1; CCRCC, clear cell renal cell carcinoma.

Article Snippet: Membranes were then incubated with rabbit polyclonal anti-ASIC1 (ASC-014; Alomone Company; 1:200 dilutions) primary antibody in blocking solution at 4°C overnight.

Techniques: Immunohistochemistry, Expressing, Staining

Journal: Pharmaceuticals

Article Title: Segmental Upregulation of ASIC1 Channels in the Formalin Acute Pain Mouse Model

doi: 10.3390/ph15121539

Figure Lengend Snippet: ASIC1 levels in the pain pathway. ( A ) Representative membrane of lysates of ACC tissue from formalin (For)- or vehicle (Veh)- injected male mice at Ip (ipsilateral) or Con (contralateral) sides to the injection detected using ASIC1 and tubulin antibodies (left), and plot of the results obtained from membranes for ASIC1/tubulin detected levels (right) (4–5 animals used per condition). ( B ) Representative membrane of lysates of SC tissue from For- or Veh- injected mice at different lumbar (3, 4, 5) levels detected using ASIC1 and tubulin antibodies (left), and plot of the results obtained from membranes for ASIC1/tubulin detected levels (right) (4–5 animals used per condition). Notice the gradient decrease from level 3 to 5. ( C ) Representative membrane of lysates of a pool of DRG tissue from different lumbar (3, 4, 5) levels in For- or Veh- injected male mice at Ip or Con sides to the injection detected using ASIC1 and tubulin antibodies. Notice the same gradient pattern as in ( B , D ) Images of the regions dissected (SC, DRG segments) used for the experiments in ( B , C ). One-way ANOVA, **** p < 0.0001; *** p < 0.001 ** p < 0.01. Scale bar 5 mm.

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti ASIC1 (Alomone ASC-014, 1:1000); mouse monoclonal anti-tubulin (DM1a; Cell signaling #3873, 1:5000); rabbit polyclonal anti-total ERK (Santa Cruz, C9, 1:500); rabbit polyclonal anti phosphoERK (Cell Signaling, SC-7383, 1:500).

Techniques: Injection

Journal: Pharmaceuticals

Article Title: Segmental Upregulation of ASIC1 Channels in the Formalin Acute Pain Mouse Model

doi: 10.3390/ph15121539

Figure Lengend Snippet: Schematic representation of ASIC1 upregulation in ACC, SC and DRG in the formalin mouse model of pain. At the cortex, the ACC contralateral to the injection (left hind paw) shows a higher ASIC1 protein level. At the SC and DRGs, there is a gradient decreasing from L3 to L5 lumbar segments. Spinal nerves contribution to the sciatic nerve is represented with lines, thicker for those contributing to a greater extent. ASIC1 protein levels are represented showing an increase with more intense color. (IP, ipsilateral; CON, contralateral; ACC, Anterior Cingulate Cortex; DRG (Dorsal Root Ganglia; SC, Spinal Cord; L3, 4, 5, lumbar 3, 4, 5).

Article Snippet: The following primary antibodies were used: rabbit polyclonal anti ASIC1 (Alomone ASC-014, 1:1000); mouse monoclonal anti-tubulin (DM1a; Cell signaling #3873, 1:5000); rabbit polyclonal anti-total ERK (Santa Cruz, C9, 1:500); rabbit polyclonal anti phosphoERK (Cell Signaling, SC-7383, 1:500).

Techniques: Injection

Journal: BMC Cancer

Article Title: ASIC1α up-regulates MMP-2/9 expression to enhance mobility and proliferation of liver cancer cells via the PI3K/AKT/mTOR pathway

doi: 10.1186/s12885-022-09874-w

Figure Lengend Snippet: ASIC1a can regulate aberrant activation of the PI3K/AKT/MTOR pathway. A1 , A2 , B1 , B2 , C1 and C2 Expression levels of p-PI3Kp85, p-mTOR(Ser2448) and p-AKT(Ser473) in different group cells were detected by western blotting. The concentration of PcTx1 was 20 nM, the concentration of LY294002 was 1 µM, the concentration of Rapamycin was 0.5 nM, the concentration of MK-2206 was 0.1 µM, and all conducted at 24 h. Data were expressed as the mean ± SD, n = 3. n.s: no significance, * P < 0.05, ** P < 0.01 and *** P < 0.001 all versus pH6.5 group

Article Snippet: Antibody against ASIC1a (#ASC-014) was purchased from Alomone Labs Company (Jerusalem, Israel).

Techniques: Activation Assay, Expressing, Western Blot, Concentration Assay

Journal: Neuroscience

Article Title: Extracellular Protons Mediate Presynaptic Homeostatic Potentiation at the Mouse Neuromuscular Junction

doi: 10.1016/j.neuroscience.2021.01.036

Figure Lengend Snippet: ASIC1 and ASIC2a are located at the mouse NMJ. A. All of the images shown are maximum projections of 16 images collected at 0.5 μm increments vertically through the field containing the NMJ. α-bungarotoxin (α -BTX) is shown in Red. ASIC2a antibodies are in Green. Arrows point to clusters of ASIC2a staining that are outside the area defining the motor nerve terminal but near perisynaptic Schwann cell nuclei, indicated by the DAPI stain (Blue). Calibration bar, 10 μm. B. All of the images shown are maximum projections of 18 images collected at 0.5 μm increments vertically through the field containing the NMJ. α-bungarotoxin (α -BTX) is shown in Red. ASIC1 antibodies are in Green. Arrows point to clusters of ASIC2a staining that are outside the area defining the motor nerve terminal but near perisynaptic Schwann cell nuclei, indicated by the DAPI stain (Blue). Calibration bar, 10 μm.

Article Snippet: However, we did observe staining with a general antibody to ASIC1 subunits (AGP-053, Alomone Labs, Jerusalem).

Techniques: Staining

Journal: Neuroscience

Article Title: Extracellular Protons Mediate Presynaptic Homeostatic Potentiation at the Mouse Neuromuscular Junction

doi: 10.1016/j.neuroscience.2021.01.036

Figure Lengend Snippet: ASIC1 and ASIC2a are located at the mouse NMJ. A. All of the images shown are maximum projections of 16 images collected at 0.5 μm increments vertically through the field containing the NMJ. α-bungarotoxin (α -BTX) is shown in Red. ASIC2a antibodies are in Green. Arrows point to clusters of ASIC2a staining that are outside the area defining the motor nerve terminal but near perisynaptic Schwann cell nuclei, indicated by the DAPI stain (Blue). Calibration bar, 10 μm. B. All of the images shown are maximum projections of 18 images collected at 0.5 μm increments vertically through the field containing the NMJ. α-bungarotoxin (α -BTX) is shown in Red. ASIC1 antibodies are in Green. Arrows point to clusters of ASIC2a staining that are outside the area defining the motor nerve terminal but near perisynaptic Schwann cell nuclei, indicated by the DAPI stain (Blue). Calibration bar, 10 μm.

Article Snippet: For ASIC1 staining we paired the guinea pig primary anti-ASIC1 antibody (AGP-053; Alomone Labs) with Goat Anti-Guinea pig Alexa Fluor 488 IgG (H±L) secondary antibody (A109FN).

Techniques: Staining

Journal: Neuroscience

Article Title: Extracellular Protons Mediate Presynaptic Homeostatic Potentiation at the Mouse Neuromuscular Junction

doi: 10.1016/j.neuroscience.2021.01.036

Figure Lengend Snippet: ASIC1 and ASIC2a are located at the mouse NMJ. A. All of the images shown are maximum projections of 16 images collected at 0.5 μm increments vertically through the field containing the NMJ. α-bungarotoxin (α -BTX) is shown in Red. ASIC2a antibodies are in Green. Arrows point to clusters of ASIC2a staining that are outside the area defining the motor nerve terminal but near perisynaptic Schwann cell nuclei, indicated by the DAPI stain (Blue). Calibration bar, 10 μm. B. All of the images shown are maximum projections of 18 images collected at 0.5 μm increments vertically through the field containing the NMJ. α-bungarotoxin (α -BTX) is shown in Red. ASIC1 antibodies are in Green. Arrows point to clusters of ASIC2a staining that are outside the area defining the motor nerve terminal but near perisynaptic Schwann cell nuclei, indicated by the DAPI stain (Blue). Calibration bar, 10 μm.

Article Snippet: For ASIC1 staining we paired the guinea pig primary anti-ASIC1 antibody (AGP-053; Alomone Labs) with Goat Anti-Guinea pig Alexa Fluor 488 IgG (H±L) secondary antibody (A109FN).

Techniques: Staining