anti ricinus communis agglutinin 1 rca 1 antibody  (Vector Laboratories)


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    Vector Laboratories anti ricinus communis agglutinin 1 rca 1 antibody
    Anti Ricinus Communis Agglutinin 1 Rca 1 Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ricinus communis agglutinin 1 rca 1 antibody/product/Vector Laboratories
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    anti ricinus communis agglutinin 1 rca 1 antibody  (Vector Laboratories)


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    Vector Laboratories anti ricinus communis agglutinin 1 rca 1 antibody
    Anti Ricinus Communis Agglutinin 1 Rca 1 Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ricinus communis agglutinin 1 rca 1 antibody/product/Vector Laboratories
    Average 92 stars, based on 1 article reviews
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    polyclonal goat anti ricinus communis agglutinin  (Vector Laboratories)


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    Vector Laboratories polyclonal goat anti ricinus communis agglutinin
    Polyclonal Goat Anti Ricinus Communis Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal goat anti ricinus communis agglutinin/product/Vector Laboratories
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    rca i antibody  (Vector Laboratories)


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    Vector Laboratories rca i antibody
    12 b bind to a glycoprotein containing α, β-D galactose. ( A , B ) Effect of 12b on RTK proteins stabilization. MDA-MB-231 cells were treated with or without 12b (4 μM) for 2 h before being heated at different temperatures. Protein levels were analyzed by Western blotting. ( C , D ) The relative band intensity to β-Tubulin was analyzed by ImageJ software. Protein band densities were quantified by normalizing to DMSO. ( E ) The samples in ( A ) were taken for <t>immunoblotting,</t> <t>membranes</t> were incubated with the <t>RCA</t> <t>I</t> antibody, and HRP-Streptavidin antibody. Signals were detected by chemiluminescence.
    Rca I Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rca i antibody/product/Vector Laboratories
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rca i antibody - by Bioz Stars, 2024-05
    92/100 stars

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    1) Product Images from "Identification of a Novel Inhibitor of TfR1 from Designed and Synthesized Muriceidine A Derivatives"

    Article Title: Identification of a Novel Inhibitor of TfR1 from Designed and Synthesized Muriceidine A Derivatives

    Journal: Antioxidants

    doi: 10.3390/antiox11050834

    12 b bind to a glycoprotein containing α, β-D galactose. ( A , B ) Effect of 12b on RTK proteins stabilization. MDA-MB-231 cells were treated with or without 12b (4 μM) for 2 h before being heated at different temperatures. Protein levels were analyzed by Western blotting. ( C , D ) The relative band intensity to β-Tubulin was analyzed by ImageJ software. Protein band densities were quantified by normalizing to DMSO. ( E ) The samples in ( A ) were taken for immunoblotting, membranes were incubated with the RCA I antibody, and HRP-Streptavidin antibody. Signals were detected by chemiluminescence.
    Figure Legend Snippet: 12 b bind to a glycoprotein containing α, β-D galactose. ( A , B ) Effect of 12b on RTK proteins stabilization. MDA-MB-231 cells were treated with or without 12b (4 μM) for 2 h before being heated at different temperatures. Protein levels were analyzed by Western blotting. ( C , D ) The relative band intensity to β-Tubulin was analyzed by ImageJ software. Protein band densities were quantified by normalizing to DMSO. ( E ) The samples in ( A ) were taken for immunoblotting, membranes were incubated with the RCA I antibody, and HRP-Streptavidin antibody. Signals were detected by chemiluminescence.

    Techniques Used: Western Blot, Software, Incubation

    ricinus communis agglutinin  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin
    Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and <t>ricinus</t> <t>communis</t> <t>agglutinin-1</t> (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )
    Ricinus Communis Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ricinus communis agglutinin/product/Vector Laboratories
    Average 92 stars, based on 1 article reviews
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    ricinus communis agglutinin - by Bioz Stars, 2024-05
    92/100 stars

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    1) Product Images from "Astroglial and oligodendroglial markers in the cuprizone animal model for de- and remyelination"

    Article Title: Astroglial and oligodendroglial markers in the cuprizone animal model for de- and remyelination

    Journal: Histochemistry and Cell Biology

    doi: 10.1007/s00418-022-02096-y

    Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )
    Figure Legend Snippet: Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )

    Techniques Used: Immunohistochemical staining, Marker, Expressing, Double Immunofluorescence Staining

    rca 1  (Vector Laboratories)


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    Vector Laboratories rca 1
    Immunohistochemical analysis of S100B and <t>RCA-1</t> (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )
    Rca 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rca 1/product/Vector Laboratories
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rca 1 - by Bioz Stars, 2024-05
    92/100 stars

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    1) Product Images from "Astroglial and oligodendroglial markers in the cuprizone animal model for de- and remyelination"

    Article Title: Astroglial and oligodendroglial markers in the cuprizone animal model for de- and remyelination

    Journal: Histochemistry and Cell Biology

    doi: 10.1007/s00418-022-02096-y

    Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )
    Figure Legend Snippet: Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )

    Techniques Used: Immunohistochemical staining, Marker, Expressing, Double Immunofluorescence Staining

    Immunohistochemical analysis of LCN2 expression during cuprizone-induced de- and remyelination in the central corpus callosum. The graph in ( a ) represents a qualitative analysis of GFAP+ astrocytes (red bars) and double GFAP+/LCN2 high + astrocytes (gray bars) during the course of de- and remyelination in the central corpus callosum (3W cup: beginning of demyelination, 3 weeks; 5W cup: severe demyelination, 5 weeks; 5W cup+2: remyelination, 7 weeks). Please note that almost all astrocytes in the central corpus callosum displayed very weak, diffuse LCN2 staining at week 5. We could not consider it conclusively as specific staining; thus we count only highly LCN2-positive astrocytes (+LCN2 high ). Black bars represent the percentages of GFAP+/LCN2 high + to/from all GFAP+ astrocytes. Bar charts show mean values and SEMs. Significant effects versus controls are indicated by rhombs ( #p < 0.05, ##p < 0.01, and ###p < 0.001) and significant effects versus different time points are indicated by a line and asterisks (* p < 0.05, **p < 0.01, and ***p < 0.001), n = 4–7. Representative pictures ( b ) demonstrate double immunofluorescence staining with LCN2 (red) and GFAP (green) performed in the central corpus callosum during cuprizone-induced de- and remyelination. LCN2 (red) does not co-localize with RCA-1 (green), shown in c . Scale bars: 20 µm ( b , c )
    Figure Legend Snippet: Immunohistochemical analysis of LCN2 expression during cuprizone-induced de- and remyelination in the central corpus callosum. The graph in ( a ) represents a qualitative analysis of GFAP+ astrocytes (red bars) and double GFAP+/LCN2 high + astrocytes (gray bars) during the course of de- and remyelination in the central corpus callosum (3W cup: beginning of demyelination, 3 weeks; 5W cup: severe demyelination, 5 weeks; 5W cup+2: remyelination, 7 weeks). Please note that almost all astrocytes in the central corpus callosum displayed very weak, diffuse LCN2 staining at week 5. We could not consider it conclusively as specific staining; thus we count only highly LCN2-positive astrocytes (+LCN2 high ). Black bars represent the percentages of GFAP+/LCN2 high + to/from all GFAP+ astrocytes. Bar charts show mean values and SEMs. Significant effects versus controls are indicated by rhombs ( #p < 0.05, ##p < 0.01, and ###p < 0.001) and significant effects versus different time points are indicated by a line and asterisks (* p < 0.05, **p < 0.01, and ***p < 0.001), n = 4–7. Representative pictures ( b ) demonstrate double immunofluorescence staining with LCN2 (red) and GFAP (green) performed in the central corpus callosum during cuprizone-induced de- and remyelination. LCN2 (red) does not co-localize with RCA-1 (green), shown in c . Scale bars: 20 µm ( b , c )

    Techniques Used: Immunohistochemical staining, Expressing, Staining, Double Immunofluorescence Staining

    lectin ricinus communis agglutinin 1  (Vector Laboratories)


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    Vector Laboratories lectin ricinus communis agglutinin 1
    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A <t>)—RCA-1</t> positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
    Lectin Ricinus Communis Agglutinin 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lectin ricinus communis agglutinin 1/product/Vector Laboratories
    Average 92 stars, based on 1 article reviews
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    lectin ricinus communis agglutinin 1 - by Bioz Stars, 2024-05
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    1) Product Images from "Regenerative Effects of CDP-Choline: A Dose-Dependent Study in the Toxic Cuprizone Model of De- and Remyelination"

    Article Title: Regenerative Effects of CDP-Choline: A Dose-Dependent Study in the Toxic Cuprizone Model of De- and Remyelination

    Journal: Pharmaceuticals

    doi: 10.3390/ph14111156

    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
    Figure Legend Snippet: Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).

    Techniques Used:

    ricinus communis agglutinin i  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin i
    Ricinus Communis Agglutinin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ricinus communis agglutinin i rca i  (Vector Laboratories)


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    Vector Laboratories ricinus communis agglutinin i rca i
    Ricinus Communis Agglutinin I Rca I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ricinus communis agglutinin i rca i/product/Vector Laboratories
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    endothelial cells goat  (Vector Laboratories)


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    Vector Laboratories endothelial cells goat
    Endothelial Cells Goat, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    goat anti ricinus communis agglutinin 1  (Vector Laboratories)


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    Vector Laboratories goat anti ricinus communis agglutinin 1
    Goat Anti Ricinus Communis Agglutinin 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories anti ricinus communis agglutinin 1 rca 1 antibody
    Anti Ricinus Communis Agglutinin 1 Rca 1 Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories polyclonal goat anti ricinus communis agglutinin
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    Vector Laboratories rca i antibody
    12 b bind to a glycoprotein containing α, β-D galactose. ( A , B ) Effect of 12b on RTK proteins stabilization. MDA-MB-231 cells were treated with or without 12b (4 μM) for 2 h before being heated at different temperatures. Protein levels were analyzed by Western blotting. ( C , D ) The relative band intensity to β-Tubulin was analyzed by ImageJ software. Protein band densities were quantified by normalizing to DMSO. ( E ) The samples in ( A ) were taken for <t>immunoblotting,</t> <t>membranes</t> were incubated with the <t>RCA</t> <t>I</t> antibody, and HRP-Streptavidin antibody. Signals were detected by chemiluminescence.
    Rca I Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rca i antibody/product/Vector Laboratories
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    Vector Laboratories ricinus communis agglutinin
    Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and <t>ricinus</t> <t>communis</t> <t>agglutinin-1</t> (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )
    Ricinus Communis Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories rca 1
    Immunohistochemical analysis of S100B and <t>RCA-1</t> (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )
    Rca 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories lectin ricinus communis agglutinin 1
    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A <t>)—RCA-1</t> positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
    Lectin Ricinus Communis Agglutinin 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories ricinus communis agglutinin i
    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A <t>)—RCA-1</t> positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
    Ricinus Communis Agglutinin I, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories ricinus communis agglutinin i rca i
    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A <t>)—RCA-1</t> positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
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    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A <t>)—RCA-1</t> positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
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    Vector Laboratories goat anti ricinus communis agglutinin 1
    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A <t>)—RCA-1</t> positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).
    Goat Anti Ricinus Communis Agglutinin 1, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    12 b bind to a glycoprotein containing α, β-D galactose. ( A , B ) Effect of 12b on RTK proteins stabilization. MDA-MB-231 cells were treated with or without 12b (4 μM) for 2 h before being heated at different temperatures. Protein levels were analyzed by Western blotting. ( C , D ) The relative band intensity to β-Tubulin was analyzed by ImageJ software. Protein band densities were quantified by normalizing to DMSO. ( E ) The samples in ( A ) were taken for immunoblotting, membranes were incubated with the RCA I antibody, and HRP-Streptavidin antibody. Signals were detected by chemiluminescence.

    Journal: Antioxidants

    Article Title: Identification of a Novel Inhibitor of TfR1 from Designed and Synthesized Muriceidine A Derivatives

    doi: 10.3390/antiox11050834

    Figure Lengend Snippet: 12 b bind to a glycoprotein containing α, β-D galactose. ( A , B ) Effect of 12b on RTK proteins stabilization. MDA-MB-231 cells were treated with or without 12b (4 μM) for 2 h before being heated at different temperatures. Protein levels were analyzed by Western blotting. ( C , D ) The relative band intensity to β-Tubulin was analyzed by ImageJ software. Protein band densities were quantified by normalizing to DMSO. ( E ) The samples in ( A ) were taken for immunoblotting, membranes were incubated with the RCA I antibody, and HRP-Streptavidin antibody. Signals were detected by chemiluminescence.

    Article Snippet: The membranes were incubated with the indicated RCA I antibody (Vector Laboratories, Inc., Burlingame, CA, USA) for 30 min at room temperature, followed by HRP-Streptavidin antibody (R&D Systems, Minneapolis, MN, USA) at room temperature for 1 h. After washing with TBST, signals were detected by chemiluminescence with the enhanced chemiluminescence (ECL) detection reagents.

    Techniques: Western Blot, Software, Incubation

    Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )

    Journal: Histochemistry and Cell Biology

    Article Title: Astroglial and oligodendroglial markers in the cuprizone animal model for de- and remyelination

    doi: 10.1007/s00418-022-02096-y

    Figure Lengend Snippet: Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )

    Article Snippet: Slides were then incubated overnight at 4 °C with the following primary antibodies: myelin proteolipid protein (PLP; mouse IgG2a, 1:500, Serotec, Bio-Rad), glial fibrillary acidic protein (GFAP; mouse IgG1, 1:200, Merck Millipore), (GFAP; rabbit, 1:200, Dako), vimentin (VIM, rabbit IgG,1:500, Abcam), aldehyde dehydrogenase (ALDH1L1, mouse IgG, 1:1000, Merck Millipore), calcium-binding protein B (S100B; rabbit IgG, 1:500, Abcam), adenomatous polyposis coli (APC, clone CC1; mouse IgG2b, 1:200, Calbiochem, Merck Millipore), lipocalin 2 (LCN2; goat IgG, 1:200, R&D), ricinus communis agglutinin (RCA-1, 1:1000, Vector Laboratories), OLIG2 (rabbit IgG,1:500, Chemicon, Merck Millipore) (Table ).

    Techniques: Immunohistochemical staining, Marker, Expressing, Double Immunofluorescence Staining

    Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )

    Journal: Histochemistry and Cell Biology

    Article Title: Astroglial and oligodendroglial markers in the cuprizone animal model for de- and remyelination

    doi: 10.1007/s00418-022-02096-y

    Figure Lengend Snippet: Immunohistochemical analysis of S100B and RCA-1 (marker for activated microglia) expression. Representative pictures demonstrate double immunofluorescence staining with S100B (red) and ricinus communis agglutinin-1 (RCA-1) (green) performed in the central corpus callosum ( a ), cortex (represented layers V–VI in ( b ), and hippocampal areas CA3 ( c ) and dentate gyrus ( d ) of C57BL/6 mice at week 5 under cuprizone diet (severe demyelination). Co-localization of S100B with RCA-1 was not found. Scale bars: 10 µm ( a , d ), 20 µm ( b , c )

    Article Snippet: RCA-1 , Lectin , 1:1000 , FL1081 , Vector Laboratories , AB_2336708 , Activated microglia.

    Techniques: Immunohistochemical staining, Marker, Expressing, Double Immunofluorescence Staining

    Immunohistochemical analysis of LCN2 expression during cuprizone-induced de- and remyelination in the central corpus callosum. The graph in ( a ) represents a qualitative analysis of GFAP+ astrocytes (red bars) and double GFAP+/LCN2 high + astrocytes (gray bars) during the course of de- and remyelination in the central corpus callosum (3W cup: beginning of demyelination, 3 weeks; 5W cup: severe demyelination, 5 weeks; 5W cup+2: remyelination, 7 weeks). Please note that almost all astrocytes in the central corpus callosum displayed very weak, diffuse LCN2 staining at week 5. We could not consider it conclusively as specific staining; thus we count only highly LCN2-positive astrocytes (+LCN2 high ). Black bars represent the percentages of GFAP+/LCN2 high + to/from all GFAP+ astrocytes. Bar charts show mean values and SEMs. Significant effects versus controls are indicated by rhombs ( #p < 0.05, ##p < 0.01, and ###p < 0.001) and significant effects versus different time points are indicated by a line and asterisks (* p < 0.05, **p < 0.01, and ***p < 0.001), n = 4–7. Representative pictures ( b ) demonstrate double immunofluorescence staining with LCN2 (red) and GFAP (green) performed in the central corpus callosum during cuprizone-induced de- and remyelination. LCN2 (red) does not co-localize with RCA-1 (green), shown in c . Scale bars: 20 µm ( b , c )

    Journal: Histochemistry and Cell Biology

    Article Title: Astroglial and oligodendroglial markers in the cuprizone animal model for de- and remyelination

    doi: 10.1007/s00418-022-02096-y

    Figure Lengend Snippet: Immunohistochemical analysis of LCN2 expression during cuprizone-induced de- and remyelination in the central corpus callosum. The graph in ( a ) represents a qualitative analysis of GFAP+ astrocytes (red bars) and double GFAP+/LCN2 high + astrocytes (gray bars) during the course of de- and remyelination in the central corpus callosum (3W cup: beginning of demyelination, 3 weeks; 5W cup: severe demyelination, 5 weeks; 5W cup+2: remyelination, 7 weeks). Please note that almost all astrocytes in the central corpus callosum displayed very weak, diffuse LCN2 staining at week 5. We could not consider it conclusively as specific staining; thus we count only highly LCN2-positive astrocytes (+LCN2 high ). Black bars represent the percentages of GFAP+/LCN2 high + to/from all GFAP+ astrocytes. Bar charts show mean values and SEMs. Significant effects versus controls are indicated by rhombs ( #p < 0.05, ##p < 0.01, and ###p < 0.001) and significant effects versus different time points are indicated by a line and asterisks (* p < 0.05, **p < 0.01, and ***p < 0.001), n = 4–7. Representative pictures ( b ) demonstrate double immunofluorescence staining with LCN2 (red) and GFAP (green) performed in the central corpus callosum during cuprizone-induced de- and remyelination. LCN2 (red) does not co-localize with RCA-1 (green), shown in c . Scale bars: 20 µm ( b , c )

    Article Snippet: RCA-1 , Lectin , 1:1000 , FL1081 , Vector Laboratories , AB_2336708 , Activated microglia.

    Techniques: Immunohistochemical staining, Expressing, Staining, Double Immunofluorescence Staining

    Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).

    Journal: Pharmaceuticals

    Article Title: Regenerative Effects of CDP-Choline: A Dose-Dependent Study in the Toxic Cuprizone Model of De- and Remyelination

    doi: 10.3390/ph14111156

    Figure Lengend Snippet: Graphs represent the qualitative analysis (cells/mm 2 ) of activated microglia (( A )—RCA-1 positive microglia; ( B )—Mac-3 positive microglia, ( C )—proliferating RCA-1/Ki-67 positive microglia), and GFAP positive astrocytes ( D ) during early remyelination (week 5.5) in the corpus callosum of animals treated with different dosages of CDP-choline or sham (PBS). The astrogliosis was not affected by CDP-choline treatment. However, the number of activated microglia in all animals treated with CDP-choline, regardless of the dose used, was significantly lower than in the control group. Significant effects between mice treated with different dosages of CDP-choline and PBS are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n = 6).

    Article Snippet: The following primary antibodies were used: for myelin proteolipid protein (PLP) (mouse monoclonal IgG2a, 1:500, Serotec) and myelin basic protein (MBP) (mouse monoclonal IgG2b, 1:500, Covance), for activated microglia Mac-3 (rat IgG, 1:500, BD Pharmingen) and the lectin ricinus communis agglutinin 1 (RCA-1) (1:1000, biotinylated, Vector Laboratories), for astrocytes glial fibrillary acidic protein (GFAP) (mouse IgG, 1:200, Millipore), for oligodendrocytes Nogo-A (rabbit IgG, 1:750, Millipore) and anti-adenomatus polyposis coli (APC; mouse IgG; 1:200).

    Techniques: