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Millipore as 601245
As 601245, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 103 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/as 601245/product/Millipore
Average 84 stars, based on 103 article reviews
Price from $9.99 to $1999.99
as 601245 - by Bioz Stars, 2020-04
84/100 stars

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Related Articles

Clone Assay:

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: An NdeI-NotI bcsZ fragment was obtained by digestion and cloned into pUA1108 , yielding plasmid pUA1108:: bcsZ , so that bcsZ expression was under the control of the tac promoter. .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

Amplification:

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: The sen3440 ( bcsZ ) coding region was amplified from S. Enteritidis 3934 chromosomal DNA using bcsZ NdeI Fw and bcsZ NotI Rv oligonucleotides containing NdeI and NotI restriction sites. .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

Construct:

Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
Article Snippet: The resulting expression constructs, encoding for the fusion proteins 6xHis-IFP-TEV-endo-β-1,4-glucanase/-endo-β-1,4-xylanase and endo-β-1,4-glucanase-/endo-β-1,4-xylanase-TEV-IFP-6xHis, respectively, were transformed into different E. coli expression strains. .. Subsequently, 2 µL of the E. coli cultures were transferred to petri dishes containing selection medium supplemented with 2 mM IPTG and 0.2% carboxymethylcellulose (CMC; Sigma-Aldrich) or birch wood xylan (Roth, Karlsruhe, Germany) for glucanase or xylanase activity assays, respectively.

Incubation:

Article Title: Analysis of Light- and Carbon-Specific Transcriptomes Implicates a Class of G-Protein-Coupled Receptors in Cellulose Sensing
Article Snippet: .. For analysis of hyphal extension, 3% (wt/vol) malt extract-agar plates and Mandels-Andreotti agar plates with 1% (wt/vol) carboxymethylcellulose (Sigma, USA) were inoculated with spore solution and incubated at room temperature in daylight. ..

Article Title: Replacement of pr gene with Japanese encephalitis virus pr using reverse genetics reduces antibody-dependent enhancement of dengue virus 2 infection
Article Snippet: .. The virus was removed and 1 ml of DMEM medium containing 2 % FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM l -glutamine, and 1.2 % (w /v ) carboxymethylcellulose (Sigma) was added, and plates were incubated further at 37 °C for 7 days. .. The cells were fixed with 4 % (v /v ) formaldehyde and the plaques were visualized by staining with 0.8 % (w /v ) crystal violet.

Article Title: Analysis of Light- and Carbon-Specific Transcriptomes Implicates a Class of G-Protein-Coupled Receptors in Cellulose Sensing
Article Snippet: .. Malt extract-agar plates (3% [wt/vol]) and Mandels-Andreotti agar plates with 1% (wt/vol) carboxymethylcellulose (Sigma, USA) were inoculated with spore solution and incubated at room temperature in daylight or constant darkness. .. For analysis of hyphal extension, 3% (wt/vol) malt extract-agar plates and Mandels-Andreotti agar plates with 1% (wt/vol) carboxymethylcellulose (Sigma, USA) were inoculated with spore solution and incubated at room temperature in daylight.

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG. .. After 16 h of incubation at 37°C, the agar medium was flooded with an aqueous solution of 1 mg/ml Congo red (Sigma) for 15 min.

Activity Assay:

Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
Article Snippet: .. Subsequently, 2 µL of the E. coli cultures were transferred to petri dishes containing selection medium supplemented with 2 mM IPTG and 0.2% carboxymethylcellulose (CMC; Sigma-Aldrich) or birch wood xylan (Roth, Karlsruhe, Germany) for glucanase or xylanase activity assays, respectively. .. After over-night growth at 37°C, plates were stained with Congo Red to detect enzyme activities, as described by Pointing .

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG. .. After 16 h of incubation at 37°C, the agar medium was flooded with an aqueous solution of 1 mg/ml Congo red (Sigma) for 15 min.

Expressing:

Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
Article Snippet: The resulting expression constructs, encoding for the fusion proteins 6xHis-IFP-TEV-endo-β-1,4-glucanase/-endo-β-1,4-xylanase and endo-β-1,4-glucanase-/endo-β-1,4-xylanase-TEV-IFP-6xHis, respectively, were transformed into different E. coli expression strains. .. Subsequently, 2 µL of the E. coli cultures were transferred to petri dishes containing selection medium supplemented with 2 mM IPTG and 0.2% carboxymethylcellulose (CMC; Sigma-Aldrich) or birch wood xylan (Roth, Karlsruhe, Germany) for glucanase or xylanase activity assays, respectively.

Article Title: β-Eudesmol, an oxygenized sesquiterpene, stimulates appetite via TRPA1 and the autonomic nervous system
Article Snippet: Thioperamide and carboxymethylcellulose were purchased from Sigma-Aldrich (MO, USA) and Calbiochem (MA, USA). .. Rat TRPA1 channel expression vector was purchased from OriGene Technologies (MD, USA).

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: An NdeI-NotI bcsZ fragment was obtained by digestion and cloned into pUA1108 , yielding plasmid pUA1108:: bcsZ , so that bcsZ expression was under the control of the tac promoter. .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

Transformation Assay:

Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
Article Snippet: The resulting expression constructs, encoding for the fusion proteins 6xHis-IFP-TEV-endo-β-1,4-glucanase/-endo-β-1,4-xylanase and endo-β-1,4-glucanase-/endo-β-1,4-xylanase-TEV-IFP-6xHis, respectively, were transformed into different E. coli expression strains. .. Subsequently, 2 µL of the E. coli cultures were transferred to petri dishes containing selection medium supplemented with 2 mM IPTG and 0.2% carboxymethylcellulose (CMC; Sigma-Aldrich) or birch wood xylan (Roth, Karlsruhe, Germany) for glucanase or xylanase activity assays, respectively.

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: Plasmid pUA1108:: bcsZ was transformed in the S. Enteritidis 3934 strain, and IPTG-mediated overexpression of bcsZ was confirmed in crude extracts by SDS-PAGE (data not shown). .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

Over Expression:

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: Paragraph title: Overexpression of bcsZ and endoglucanase assay. ... To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

Activation Assay:

Article Title: The proinflammatory cytokines IL-1? and TNF-? induce the expression of Synoviolin, an E3 ubiquitin ligase, in mouse synovial fibroblasts via the Erk1/2-ETS1 pathway
Article Snippet: .. Reagents, antibodies, and plasmids All the mitogen-activated protein kinase (MAPK) inhibitors used in this study, including the Erk activation inhibitor, PD98059 [ ], the c-Jun N-terminal kinase (JNK) inhibitor, SP600125 [ ], p38 inhibitor, SB202190 [ ], and the NF-κB inhibitor, SN50 [ ], were purchased from Calbiochem (San Diego, CA, USA). .. Antibodies against JNK1, Erk, and p38 were purchased from Promega Corporation (Madison, WI, USA).

Article Title: Sequential treatment with celecoxib and bortezomib enhances the ER stress-mediated autophagy-associated cell death of colon cancer cells
Article Snippet: SP600125, a c-Jun N-terminal kinase (JNK) inhibitor, SB203580, a p38-mitogen-activated protein kinase (MAPK) inhibitor and salubrinal, an ER stress inhibiter, were purchased from Calbiochem (Merck KGaA, Darmstadt, Germany). .. To inhibit the activation of JNK or p38-mitogen-activated protein kinase, cells were pre-treated with SP600125 (20 µM) or SB203580 (10 µM) for 2 h at 37°C.

Cell Culture:

Article Title: Expression of Fibroblast Growth Factor 21 and β-Klotho Regulates Hepatic Fibrosis through the Nuclear Factor-κB and c-Jun N-Terminal Kinase Pathways
Article Snippet: Paragraph title: 3. Cell culture ... Cells were starved in serum-free medium for 12 hours before being treated with interleukin 1β (IL-1β; R & D Systems, Minneapolis, MN, USA), NK-κB inhibitor (Bay 11-7082; Sigma-Aldrich, St. Louis, MO, USA), c-Jun N-terminal kinase (JNK) inhibitor (SP600125, Sigma-Aldrich), phosphatidylinositol 3-kinase (PI3-K) inhibitor (LY294002, Sigma-Aldrich), mitogen-activated protein kinase kinase (MAPKK) inhibitor (PD98059, Sigma-Aldrich), and FGF21 (R & D Systems).

Article Title: Replacement of pr gene with Japanese encephalitis virus pr using reverse genetics reduces antibody-dependent enhancement of dengue virus 2 infection
Article Snippet: Plaque-forming assay BHK-21 cells were plated at a density of 1 × 105 cells per well in 24-well plates containing 500 μl of medium and cultured overnight at 37 °C. .. The virus was removed and 1 ml of DMEM medium containing 2 % FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM l -glutamine, and 1.2 % (w /v ) carboxymethylcellulose (Sigma) was added, and plates were incubated further at 37 °C for 7 days.

Inhibition:

Article Title: Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage
Article Snippet: In some experiments, omeprazole (60 mg/kg; Sigma) or famotidine (30 mg/kg; Sigma) was injected intraperitoneally prior to surgery and at least 30 min after the drug has elapsed prior to imaging to allow inhibition of gastric acid secretion. .. Omeprazole was suspended via sonication in 0.5% carboxymethylcellulose (CMC; Sigma), while famotidine was suspended in 0.5% CMC without sonication.

Imaging:

Article Title: Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage
Article Snippet: In some experiments, omeprazole (60 mg/kg; Sigma) or famotidine (30 mg/kg; Sigma) was injected intraperitoneally prior to surgery and at least 30 min after the drug has elapsed prior to imaging to allow inhibition of gastric acid secretion. .. Omeprazole was suspended via sonication in 0.5% carboxymethylcellulose (CMC; Sigma), while famotidine was suspended in 0.5% CMC without sonication.

Polymerase Chain Reaction:

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: The PCR product was cloned into the pGEM-T Easy (Promega) vector and confirmed by sequencing. .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

Sonication:

Article Title: Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage
Article Snippet: .. Omeprazole was suspended via sonication in 0.5% carboxymethylcellulose (CMC; Sigma), while famotidine was suspended in 0.5% CMC without sonication. ..

Injection:

Article Title: Inhibitors of acid secretion can benefit gastric wound repair independent of luminal pH effects on the site of damage
Article Snippet: In some experiments, omeprazole (60 mg/kg; Sigma) or famotidine (30 mg/kg; Sigma) was injected intraperitoneally prior to surgery and at least 30 min after the drug has elapsed prior to imaging to allow inhibition of gastric acid secretion. .. Omeprazole was suspended via sonication in 0.5% carboxymethylcellulose (CMC; Sigma), while famotidine was suspended in 0.5% CMC without sonication.

Article Title: Targeted Delivery of Antiglaucoma Drugs to the Supraciliary Space Using Microneedles
Article Snippet: .. For supraciliary injection, the solution was diluted to a range of drug concentrations and included 2% carboxymethylcellulose (CMC; Sigma-Aldrich, St. Louis, MO, USA) to increase viscosity and thereby improve localization of the drug at the site of injection. ..

Mouse Assay:

Article Title: Resolvin D2 Restrains Th1 Immunity and Prevents Alveolar Bone Loss in Murine Periodontitis
Article Snippet: In brief, mice were treated with trimethoprim (0.16 mg/mL) and sulfamethoxazole (0.8 mg/mL) solution (Resprim; Teva Pharmaceutical Industries) in the drinking water for 10 days, followed by 3 days without antibiotics. .. 1 × 109 colony-forming units of P. gingivalis 53977 were introduced via oral gavage, three times at 2-day intervals in 200 µL of 2% (wt/vol) carboxymethylcellulose (CMC) solution (Sigma).

Sequencing:

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: The PCR product was cloned into the pGEM-T Easy (Promega) vector and confirmed by sequencing. .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

SDS Page:

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: Plasmid pUA1108:: bcsZ was transformed in the S. Enteritidis 3934 strain, and IPTG-mediated overexpression of bcsZ was confirmed in crude extracts by SDS-PAGE (data not shown). .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

Plasmid Preparation:

Article Title: β-Eudesmol, an oxygenized sesquiterpene, stimulates appetite via TRPA1 and the autonomic nervous system
Article Snippet: Thioperamide and carboxymethylcellulose were purchased from Sigma-Aldrich (MO, USA) and Calbiochem (MA, USA). .. Rat TRPA1 channel expression vector was purchased from OriGene Technologies (MD, USA).

Article Title: Coordinated Cyclic-Di-GMP Repression of Salmonella Motility through YcgR and Cellulose
Article Snippet: Plasmid pUA1108:: bcsZ was transformed in the S. Enteritidis 3934 strain, and IPTG-mediated overexpression of bcsZ was confirmed in crude extracts by SDS-PAGE (data not shown). .. To assay the endoglucanase activity of bcsZ , bacterial colonies were grown on LB agar plates containing 0.1% of carboxymethylcellulose (CMC) (Sigma) and 70 μM IPTG.

Selection:

Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
Article Snippet: .. Subsequently, 2 µL of the E. coli cultures were transferred to petri dishes containing selection medium supplemented with 2 mM IPTG and 0.2% carboxymethylcellulose (CMC; Sigma-Aldrich) or birch wood xylan (Roth, Karlsruhe, Germany) for glucanase or xylanase activity assays, respectively. .. After over-night growth at 37°C, plates were stained with Congo Red to detect enzyme activities, as described by Pointing .

Concentration Assay:

Article Title: Targeted Delivery of Antiglaucoma Drugs to the Supraciliary Space Using Microneedles
Article Snippet: For topical delivery, the final concentration was 0.05 mg/mL sulprostone or 1.5 mg/mL brimonidine tartrate. .. For supraciliary injection, the solution was diluted to a range of drug concentrations and included 2% carboxymethylcellulose (CMC; Sigma-Aldrich, St. Louis, MO, USA) to increase viscosity and thereby improve localization of the drug at the site of injection.

Staining:

Article Title: High-Throughput Protein Expression Using a Combination of Ligation-Independent Cloning (LIC) and Infrared Fluorescent Protein (IFP) Detection
Article Snippet: Subsequently, 2 µL of the E. coli cultures were transferred to petri dishes containing selection medium supplemented with 2 mM IPTG and 0.2% carboxymethylcellulose (CMC; Sigma-Aldrich) or birch wood xylan (Roth, Karlsruhe, Germany) for glucanase or xylanase activity assays, respectively. .. After over-night growth at 37°C, plates were stained with Congo Red to detect enzyme activities, as described by Pointing .

Article Title: Replacement of pr gene with Japanese encephalitis virus pr using reverse genetics reduces antibody-dependent enhancement of dengue virus 2 infection
Article Snippet: The virus was removed and 1 ml of DMEM medium containing 2 % FBS, 100 U/ml penicillin, 100 μg/ml streptomycin, 2 mM l -glutamine, and 1.2 % (w /v ) carboxymethylcellulose (Sigma) was added, and plates were incubated further at 37 °C for 7 days. .. The cells were fixed with 4 % (v /v ) formaldehyde and the plaques were visualized by staining with 0.8 % (w /v ) crystal violet.

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  • 95
    Millipore jnk inhibitor v
    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM <t>JNK</t> <t>inhibitor</t> V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P
    Jnk Inhibitor V, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jnk inhibitor v/product/Millipore
    Average 95 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    jnk inhibitor v - by Bioz Stars, 2020-04
    95/100 stars
      Buy from Supplier

    84
    Millipore as 601245
    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM <t>JNK</t> <t>inhibitor</t> V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P
    As 601245, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/as 601245/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    as 601245 - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    85
    Millipore jnk inhibitor 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile
    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM <t>JNK</t> <t>inhibitor</t> V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P
    Jnk Inhibitor 1 3 Benzothiazol 2 Yl 2 2 3 Pyridinyl Ethyl Amino 4 Pyrimidinyl Acetonitrile, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jnk inhibitor 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jnk inhibitor 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    84
    Millipore as 601245 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile
    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM <t>JNK</t> <t>inhibitor</t> V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P
    As 601245 1 3 Benzothiazol 2 Yl 2 2 3 Pyridinyl Ethyl Amino 4 Pyrimidinyl Acetonitrile, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/as 601245 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile/product/Millipore
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    as 601245 1 3 benzothiazol 2 yl 2 2 3 pyridinyl ethyl amino 4 pyrimidinyl acetonitrile - by Bioz Stars, 2020-04
    84/100 stars
      Buy from Supplier

    Image Search Results


    POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM JNK inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P

    Journal: Molecular Cancer

    Article Title: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

    doi: 10.1186/s12943-015-0374-5

    Figure Lengend Snippet: POH-induced apoptosis in U251 cells. U251 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM JNK inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P

    Article Snippet: Cell death assay U87 and U251 cells were pretreated for 30 minutes with JNK inhibitor V [1,3-Benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; Calbiochem], an inhibitor of JNK1/2 activation, before treatment with 0.5 mM POH and 0.5 mM POH plus 0.5 μM JNK inhibitor V. After 24 hours of incubation, the cells were suspended in annexin and propidium iodide binding buffer as specified in the TACS Annexin V-FITC apoptosis detection kit (R & D Systems).

    Techniques:

    The effects of JNK inhibition on the induction of cell death by POH in U251 cells. Before treatment, U251 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A) , 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C) , and 0.5 mM POH plus JNK inhibitor V (D) . After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 7E represents the percentage of dead cells as indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 7A-D. The data were expressed as the means ± SD from at least three different experiments. ***p

    Journal: Molecular Cancer

    Article Title: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

    doi: 10.1186/s12943-015-0374-5

    Figure Lengend Snippet: The effects of JNK inhibition on the induction of cell death by POH in U251 cells. Before treatment, U251 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A) , 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C) , and 0.5 mM POH plus JNK inhibitor V (D) . After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 7E represents the percentage of dead cells as indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 7A-D. The data were expressed as the means ± SD from at least three different experiments. ***p

    Article Snippet: Cell death assay U87 and U251 cells were pretreated for 30 minutes with JNK inhibitor V [1,3-Benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; Calbiochem], an inhibitor of JNK1/2 activation, before treatment with 0.5 mM POH and 0.5 mM POH plus 0.5 μM JNK inhibitor V. After 24 hours of incubation, the cells were suspended in annexin and propidium iodide binding buffer as specified in the TACS Annexin V-FITC apoptosis detection kit (R & D Systems).

    Techniques: Inhibition, Incubation, Staining, Flow Cytometry, Cytometry

    POH-induced apoptosis in U87 cells. U87 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM JNK inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P

    Journal: Molecular Cancer

    Article Title: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

    doi: 10.1186/s12943-015-0374-5

    Figure Lengend Snippet: POH-induced apoptosis in U87 cells. U87 in the control condition (Control, A) . The cells were treated with 0.5 mM POH (POH, B) and 0.5 mM POH plus 0.5 μM JNK inhibitor V (POH + IJNK, C) . After 24 hours, the cells were immunostained for cleaved caspase-3 and the number of positive cells was analyzed (D) . Whereas POH induced cell apoptosis, the addition of the JNK inhibitor completely inhibited this effect. The addition of DMSO or JNK inhibitor V alone had no effect on cell death. Scale bar: 20 μm.*P

    Article Snippet: Cell death assay U87 and U251 cells were pretreated for 30 minutes with JNK inhibitor V [1,3-Benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; Calbiochem], an inhibitor of JNK1/2 activation, before treatment with 0.5 mM POH and 0.5 mM POH plus 0.5 μM JNK inhibitor V. After 24 hours of incubation, the cells were suspended in annexin and propidium iodide binding buffer as specified in the TACS Annexin V-FITC apoptosis detection kit (R & D Systems).

    Techniques:

    The effects of JNK inhibition on the induction of cell death by POH in U87 cells. Before treatment, U87 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A), 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C) , and 0.5 mM POH plus JNK inhibitor V (D) . After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 6E represents the percentage of dead cells indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 6A-D. The data were expressed as the means ± SD from at least three different experiments. ***p

    Journal: Molecular Cancer

    Article Title: Na/K-ATPase as a target for anticancer drugs: studies with perillyl alcohol

    doi: 10.1186/s12943-015-0374-5

    Figure Lengend Snippet: The effects of JNK inhibition on the induction of cell death by POH in U87 cells. Before treatment, U87 cells were incubated without (A and B) or with (C and D) JNK inhibitor V (0.5 μM) for 30 minutes. The cells were treated with 0.1% DMSO (A), 0.5 mM POH (B) 0.1% DMSO plus JNK inhibitor V (C) , and 0.5 mM POH plus JNK inhibitor V (D) . After 24 hours of incubation, the cells were stained with annexin V-FITC and propidium iodide and analyzed by flow cytometry. Figure 6E represents the percentage of dead cells indicated by early apoptosis and late apoptosis or necrosis (right lower quadrant + right upper quadrant, respectively), which was calculated from the data shown in Figures 6A-D. The data were expressed as the means ± SD from at least three different experiments. ***p

    Article Snippet: Cell death assay U87 and U251 cells were pretreated for 30 minutes with JNK inhibitor V [1,3-Benzothiazol-2-yl-(2-((2-(3-pyridinyl)ethyl)amino)-4-pyrimidinyl)acetonitrile; Calbiochem], an inhibitor of JNK1/2 activation, before treatment with 0.5 mM POH and 0.5 mM POH plus 0.5 μM JNK inhibitor V. After 24 hours of incubation, the cells were suspended in annexin and propidium iodide binding buffer as specified in the TACS Annexin V-FITC apoptosis detection kit (R & D Systems).

    Techniques: Inhibition, Incubation, Staining, Flow Cytometry, Cytometry