arraycontrol rna spikes  (Thermo Fisher)


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    Name:
    ArrayControl RNA Spikes
    Description:
    Ambion ArrayControl RNA Spikes consist of 8 individual RNA transcripts ranging in size from 750 to 2 000 bases with a 30 base 3 poly A tail RNA Spikes are added to the RNA sample and used as template for preparing labeled cDNA Each transcript is supplied in one tube containing 1 µg • For the normalization and control of microarray experiments • For array printing and protocol assessment optimization • Monitor reverse transcription and cDNA labeling efficiency hybridization stringency and background • Normalize Cy 3 and Cy 5 signals When hybridized to complementary spots on a gene array ArrayControl RNA Spikes can be used to monitor the efficiency of reverse transcription and aRNA amplification labeling and hybridization They can also be used to assess the precision accuracy and consistency of array printing and protocol sensitivity In addition RNA Spikes are an invaluable tool for protocol optimization and troubleshooting They can be used for normalization of Cy 3 and Cy 5 signals determination of dynamic range and slide orientation Individual RNA Spikes can also be omitted to serve as a negative control for background calculations The RNA Spike sequences have been carefully chosen to lack homology to mammalian sequences that might otherwise lead to cross hybridization to gene specific spots Each RNA Spike is purified precisely quantitated by spectrophotometry and RiboGreen staining and tested for stability under highly stringent conditions They are also tested for cDNA labeling efficiency using both direct and indirect methods Their manufacturing and quantitation are consistent from lot to lot so that signals can be compared between experiments and to assess array reproducibility To obtain RNA Spike sequence information for the preparation of complementary control spots on your arrays please contact Technical Support
    Catalog Number:
    am1780
    Price:
    None
    Applications:
    RNAi, Epigenetics & Non-Coding RNA Research|miRNA & Non-Coding RNA Analysis|Microarray Hybridization & General Reagents
    Category:
    Standards Ladders Controls
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    Structured Review

    Thermo Fisher arraycontrol rna spikes
    <t>Total-RNA-based</t> performance test (two-step) workflow and pipetting map . (A) Workflow to prime Polaris IFC with beads, back-load them with RNAspikes 147, simulate front dosing with diluted ERCC <t>spikes,</t> and generate cDNA using mRNA-seq chemistry. The cDNA amplicons from Polaris IFC were analyzed on the Fluidigm M96.96 IFC using 85 qPCR assays for specific genes, 8 assays for ERCC RNA spikes, and 3 assays for RNAspikes 147. (B) Pipetting map for IFC prime step. During prime step, RNAspikes 147 are back-loaded to 8 specific inlets with cell capture beads. (C) Pipetting map for front-dosing simulation with ERCC RNA spikes, followed by mRNA-seq chemistry.
    Ambion ArrayControl RNA Spikes consist of 8 individual RNA transcripts ranging in size from 750 to 2 000 bases with a 30 base 3 poly A tail RNA Spikes are added to the RNA sample and used as template for preparing labeled cDNA Each transcript is supplied in one tube containing 1 µg • For the normalization and control of microarray experiments • For array printing and protocol assessment optimization • Monitor reverse transcription and cDNA labeling efficiency hybridization stringency and background • Normalize Cy 3 and Cy 5 signals When hybridized to complementary spots on a gene array ArrayControl RNA Spikes can be used to monitor the efficiency of reverse transcription and aRNA amplification labeling and hybridization They can also be used to assess the precision accuracy and consistency of array printing and protocol sensitivity In addition RNA Spikes are an invaluable tool for protocol optimization and troubleshooting They can be used for normalization of Cy 3 and Cy 5 signals determination of dynamic range and slide orientation Individual RNA Spikes can also be omitted to serve as a negative control for background calculations The RNA Spike sequences have been carefully chosen to lack homology to mammalian sequences that might otherwise lead to cross hybridization to gene specific spots Each RNA Spike is purified precisely quantitated by spectrophotometry and RiboGreen staining and tested for stability under highly stringent conditions They are also tested for cDNA labeling efficiency using both direct and indirect methods Their manufacturing and quantitation are consistent from lot to lot so that signals can be compared between experiments and to assess array reproducibility To obtain RNA Spike sequence information for the preparation of complementary control spots on your arrays please contact Technical Support
    https://www.bioz.com/result/arraycontrol rna spikes/product/Thermo Fisher
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    arraycontrol rna spikes - by Bioz Stars, 2020-09
    99/100 stars

    Images

    1) Product Images from "Fluidic Logic Used in a Systems Approach to Enable Integrated Single-Cell Functional Analysis"

    Article Title: Fluidic Logic Used in a Systems Approach to Enable Integrated Single-Cell Functional Analysis

    Journal: Frontiers in Bioengineering and Biotechnology

    doi: 10.3389/fbioe.2016.00070

    Total-RNA-based performance test (two-step) workflow and pipetting map . (A) Workflow to prime Polaris IFC with beads, back-load them with RNAspikes 147, simulate front dosing with diluted ERCC spikes, and generate cDNA using mRNA-seq chemistry. The cDNA amplicons from Polaris IFC were analyzed on the Fluidigm M96.96 IFC using 85 qPCR assays for specific genes, 8 assays for ERCC RNA spikes, and 3 assays for RNAspikes 147. (B) Pipetting map for IFC prime step. During prime step, RNAspikes 147 are back-loaded to 8 specific inlets with cell capture beads. (C) Pipetting map for front-dosing simulation with ERCC RNA spikes, followed by mRNA-seq chemistry.
    Figure Legend Snippet: Total-RNA-based performance test (two-step) workflow and pipetting map . (A) Workflow to prime Polaris IFC with beads, back-load them with RNAspikes 147, simulate front dosing with diluted ERCC spikes, and generate cDNA using mRNA-seq chemistry. The cDNA amplicons from Polaris IFC were analyzed on the Fluidigm M96.96 IFC using 85 qPCR assays for specific genes, 8 assays for ERCC RNA spikes, and 3 assays for RNAspikes 147. (B) Pipetting map for IFC prime step. During prime step, RNAspikes 147 are back-loaded to 8 specific inlets with cell capture beads. (C) Pipetting map for front-dosing simulation with ERCC RNA spikes, followed by mRNA-seq chemistry.

    Techniques Used: Real-time Polymerase Chain Reaction

    Related Articles

    Amplification:

    Article Title: The Baltic Sea Virome: Diversity and Transcriptional Activity of DNA and RNA Viruses
    Article Snippet: .. A total of 107 copies of ArrayControl RNA spikes (Life Technologies, Inc., Carlsbad, CA) were added to each sample prior to ScriptSeq amplification. .. AMPure XP beads (Beckman Coulter, Inc.) were used for cDNA and final library purification.

    Labeling:

    Article Title: The Sterolgene v0 cDNA microarray: a systemic approach to studies of cholesterol homeostasis and drug metabolism
    Article Snippet: .. Before labeling spike RNAs were added to the total RNA in following quantities: 500 pg of Firef ly luciferase mRNA (Promega, Madison, WI, USA); and one of the two mixes (reference mix and sample mix) of 8 RNAs from ArrayControl Spikes (Ambion, Austin, TX, USA). ..

    Purification:

    Article Title: Transcriptomic and proteomic data in developing tomato fruit
    Article Snippet: .. To determine the absolute concentration of transcript after transcriptome sequencing, eight internal standards (AM 1780, Ambion by Life technologies, Array Control RNA spikes, Invitrogen™) at selected concentrations (in mole, 3.97.10−14 [spike 1], 4.01.10−15 [spike 2], 4.01.10−16 [spike 3], 4.02.10−17 [spike 4], 4.08.10−18 [spike 5], 4.04.10−19 [spike 6], 3.82.10−20 [spike 7], and 3.82.10−21 [spike 8]) were spiked-in the plant extracts at the beginning of the RNA purification process. .. 2.2.2 Transcript sequencing RNA-seq libraries were prepared according to Illumina's protocols on a Tecan EVO200 liquid handler using the Illumina TruSeq Stranded mRNA sample prep kit to analyze mRNA.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Gene expression analysis of skin grafts and cultured keratinocytes using synthetic RNA normalization reveals insights into differentiation and growth control
    Article Snippet: .. Equal amount of the SPIKE-in RNA mix (ArrayControl RNA, AM1780M, Ambion) was added to each cDNA synthesis reaction according to the STRT-library preparation protocol described above. cDNA synthesis was carried out using oligo dT-primers and SuperScript III First-Strand synthesis system for RT-PCR (18080–151, Invitrogen) according to manufacturer’s instructions. .. 5 ng of cDNA was applied to each qPCR assay and each sample was run in three technical replicates. qPCR was carried out using an ABI PRISM 7500 Fast Real-Time PCR System with Fast SYBR® Green Master mix (4385617, both from Applied Biosystems) according to manufacturer’s instructions.

    Concentration Assay:

    Article Title: Transcriptomic and proteomic data in developing tomato fruit
    Article Snippet: .. To determine the absolute concentration of transcript after transcriptome sequencing, eight internal standards (AM 1780, Ambion by Life technologies, Array Control RNA spikes, Invitrogen™) at selected concentrations (in mole, 3.97.10−14 [spike 1], 4.01.10−15 [spike 2], 4.01.10−16 [spike 3], 4.02.10−17 [spike 4], 4.08.10−18 [spike 5], 4.04.10−19 [spike 6], 3.82.10−20 [spike 7], and 3.82.10−21 [spike 8]) were spiked-in the plant extracts at the beginning of the RNA purification process. .. 2.2.2 Transcript sequencing RNA-seq libraries were prepared according to Illumina's protocols on a Tecan EVO200 liquid handler using the Illumina TruSeq Stranded mRNA sample prep kit to analyze mRNA.

    Luciferase:

    Article Title: The Sterolgene v0 cDNA microarray: a systemic approach to studies of cholesterol homeostasis and drug metabolism
    Article Snippet: .. Before labeling spike RNAs were added to the total RNA in following quantities: 500 pg of Firef ly luciferase mRNA (Promega, Madison, WI, USA); and one of the two mixes (reference mix and sample mix) of 8 RNAs from ArrayControl Spikes (Ambion, Austin, TX, USA). ..

    Expressing:

    Article Title: Complement in Human Pre-implantation Embryos: Attack and Defense
    Article Snippet: .. Expression levels were correlated to eight synthetic spike-in RNAs (ArrayControl RNA spikes Ambion, cat. no. AM1780) ( ). .. Following amplification, the synthesized cDNA was sequenced on the Illumina platform, filtered, demultiplexed and trimmed as described ( ).

    Sequencing:

    Article Title: Transcriptomic and proteomic data in developing tomato fruit
    Article Snippet: .. To determine the absolute concentration of transcript after transcriptome sequencing, eight internal standards (AM 1780, Ambion by Life technologies, Array Control RNA spikes, Invitrogen™) at selected concentrations (in mole, 3.97.10−14 [spike 1], 4.01.10−15 [spike 2], 4.01.10−16 [spike 3], 4.02.10−17 [spike 4], 4.08.10−18 [spike 5], 4.04.10−19 [spike 6], 3.82.10−20 [spike 7], and 3.82.10−21 [spike 8]) were spiked-in the plant extracts at the beginning of the RNA purification process. .. 2.2.2 Transcript sequencing RNA-seq libraries were prepared according to Illumina's protocols on a Tecan EVO200 liquid handler using the Illumina TruSeq Stranded mRNA sample prep kit to analyze mRNA.

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  • News
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  • Team
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  • Bioz Stars
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  • 99
    Thermo Fisher arraycontrol rna spikes
    <t>Total-RNA-based</t> performance test (two-step) workflow and pipetting map . (A) Workflow to prime Polaris IFC with beads, back-load them with RNAspikes 147, simulate front dosing with diluted ERCC <t>spikes,</t> and generate cDNA using mRNA-seq chemistry. The cDNA amplicons from Polaris IFC were analyzed on the Fluidigm M96.96 IFC using 85 qPCR assays for specific genes, 8 assays for ERCC RNA spikes, and 3 assays for RNAspikes 147. (B) Pipetting map for IFC prime step. During prime step, RNAspikes 147 are back-loaded to 8 specific inlets with cell capture beads. (C) Pipetting map for front-dosing simulation with ERCC RNA spikes, followed by mRNA-seq chemistry.
    Arraycontrol Rna Spikes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arraycontrol rna spikes/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    arraycontrol rna spikes - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Total-RNA-based performance test (two-step) workflow and pipetting map . (A) Workflow to prime Polaris IFC with beads, back-load them with RNAspikes 147, simulate front dosing with diluted ERCC spikes, and generate cDNA using mRNA-seq chemistry. The cDNA amplicons from Polaris IFC were analyzed on the Fluidigm M96.96 IFC using 85 qPCR assays for specific genes, 8 assays for ERCC RNA spikes, and 3 assays for RNAspikes 147. (B) Pipetting map for IFC prime step. During prime step, RNAspikes 147 are back-loaded to 8 specific inlets with cell capture beads. (C) Pipetting map for front-dosing simulation with ERCC RNA spikes, followed by mRNA-seq chemistry.

    Journal: Frontiers in Bioengineering and Biotechnology

    Article Title: Fluidic Logic Used in a Systems Approach to Enable Integrated Single-Cell Functional Analysis

    doi: 10.3389/fbioe.2016.00070

    Figure Lengend Snippet: Total-RNA-based performance test (two-step) workflow and pipetting map . (A) Workflow to prime Polaris IFC with beads, back-load them with RNAspikes 147, simulate front dosing with diluted ERCC spikes, and generate cDNA using mRNA-seq chemistry. The cDNA amplicons from Polaris IFC were analyzed on the Fluidigm M96.96 IFC using 85 qPCR assays for specific genes, 8 assays for ERCC RNA spikes, and 3 assays for RNAspikes 147. (B) Pipetting map for IFC prime step. During prime step, RNAspikes 147 are back-loaded to 8 specific inlets with cell capture beads. (C) Pipetting map for front-dosing simulation with ERCC RNA spikes, followed by mRNA-seq chemistry.

    Article Snippet: In the first step, the control lines on the Polaris IFC are primed, channels are blocked, and cell capture beads are back-loaded with ArrayControl RNA SPIKES (1, 4, and 7 only, Thermo Fisher Scientific, AM1780; henceforth referred to as RNAspikes 147) in eight specific capture sites (Figure B).

    Techniques: Real-time Polymerase Chain Reaction