Journal: Frontiers in immunology

Article Title: Archetypal type II and III Toxoplasma gondii oocysts induce different immune responses and clinical outcomes in experimentally infected piglets.

doi: 10.3389/fimmu.2022.1021556

Figure Lengend Snippet: FIGURE 4 Evaluation of the IgG antibody levels by ELISA in sera from piglets infected with T. gondii oocysts from the TgShSp1 (G1) and TgShSp24 (G2) isolates and the non-infected group (G3). (A) Mean IgG levels by Priochek test are represented as the percentage of positivity (%PP). (B) Mean IgG levels by ELISA in house are represented as relative index percent (RIPC); (C) Mean RIPC IgG1 antibody levels; (D) Mean RIPC IgG2 antibody levels. Error bars represent SD. Significant differences between infected groups are indicated with asterisks, where *, ** and **** denote p < 0.05, p < 0.01 and p < 0.0001, respectively.

Article Snippet: Cytokine level quantification (GM-CSF, IFN-g, IL-1b, IL-4, IL6, IL-8, IL-10, IL-12, TGF-b1, and TNF-a) was assessed in cell-free culture supernatants using a commercial array-based multiplex ELISA kit (Porcine cytokine array Q1, RayBiotech, Norcross, GA, USA) following the manufacturer ́s recommendations.

Techniques: Enzyme-linked Immunosorbent Assay, Infection

Journal: Cancers

Article Title: The Genomic Landscape in Philadelphia-Negative Myeloproliferative Neoplasm Patients with Second Cancers

doi: 10.3390/cancers14143435

Figure Lengend Snippet: Gene ontology functional analysis and cytokine measurement in both groups of MPN patients. ( A ) Gene ontology functional analysis of genes with variants in the control group (left) and the SC group (right). Immune-related pathways are highlighted in light-blue color. Pathways pertinent to epigenetic regulation and genomic stability are highlighted in red. ( B ) Differentially involved pathways linked to the mutated genes in both groups. Some key inflammation pathways enriched in SC samples are highlighted in blue. The Reactome was applied for this analysis. ( C ) Comparison of TGF-β and IL-23 levels in the two groups of MPN patients. ( D ) Comparison of IL-1β and TGF-β levels in the indicated groups of MPN patients. *: p < 0.05; **: p < 0.01 by Student’s t -test.

Article Snippet: To quantify plasma cytokine levels, the multiplex ELISA-based Q-PlexTM Human Cytokine HS Screen Array (Quansys Biosciences, Logan, Utah, USA) was used.

Techniques: Functional Assay, Control, Comparison

Journal: Journal of the Formosan Medical Association = Taiwan yi zhi

Article Title: Real-world experience with Ropeginterferon-alpha 2b (Besremi) in Philadelphia-negative myeloproliferative neoplasms.

doi: 10.1016/j.jfma.2020.08.021

Figure Lengend Snippet: Figure 4 Effects of Ropeg on cytokine profiles. (A) Relative change in the percentages of levels of five cytokines over time in one particular patient. The plasma cytokine levels were measured with multiplex ELISA-based Q-plex Human Cyokine HS Screen Array (Quansys Biosciences), which contained more than a dozen of cytokines. Only cytokines with significantly altered levels after treatment are shown here. (B) The absolute values of plasma hepcidin levels over time in six PV patients. Human Hepcidin ELISA Kit (Cusabio Technology) was used for the quantification.

Article Snippet: The plasma cytokine levels were measured with multiplex ELISA-based Q-plex Human Cyokine HS Screen Array (Quansys Biosciences), which contained more than a dozen of cytokines.

Techniques: Clinical Proteomics, Multiplex Assay, Enzyme-linked Immunosorbent Assay