arp2 3 ab  (Cell Signaling Technology Inc)

Journal: Stem Cells International

Article Title: Bone Marrow-Derived CD44 + Cells Migrate to Tissue-Engineered Constructs via SDF-1/CXCR4-JNK Pathway and Aid Bone Repair

doi: 10.1155/2019/1513526

Figure Lengend Snippet: Primers used for RT-PCR.

Article Snippet: Arp2/3 Ab , Western blot , 0.1 μ g/ml , 24 h , CST, USA.

Techniques: Sequencing

Journal: Stem Cells International

Article Title: Bone Marrow-Derived CD44 + Cells Migrate to Tissue-Engineered Constructs via SDF-1/CXCR4-JNK Pathway and Aid Bone Repair

doi: 10.1155/2019/1513526

Figure Lengend Snippet: Reagents used for in vitro experiments.

Article Snippet: Arp2/3 Ab , Western blot , 0.1 μ g/ml , 24 h , CST, USA.

Techniques: In Vitro, Concentration Assay, FACS, Flow Cytometry, Immunofluorescence, Western Blot

Journal: Stem Cells International

Article Title: Bone Marrow-Derived CD44 + Cells Migrate to Tissue-Engineered Constructs via SDF-1/CXCR4-JNK Pathway and Aid Bone Repair

doi: 10.1155/2019/1513526

Figure Lengend Snippet: JNK served as an effector downstream of SDF-1/CXCR4 in the migration of bone marrow (BM) CD44 + cells towards tissue-engineered constructs (TECs). (a) Comparison of phosphorylated JNK (p-JNK), ERK (p-ERK), and P38 (p-P38) expression in BM cells after CXCR4 blockade. After migration stopped, BM cells were collected and analyzed by western blot. (b) Representative images of cell migration towards SDF-1. The quantification of migrated BM cells is shown as a bar graph ( n = 5). ∗∗ P < 0.01. (c) FACS analysis of the proportions of CD44 + cells in peripheral blood (PB). The quantification comparison is shown as a bar graph ( n = 3). ∗∗ P < 0.01. (d) Representative images of in vivo migration of GFP + /CD44 + cells towards TECs. The introduction of SP600125 significantly reduced the recruitment of GFP + /CD44 + cells. White triangle, implant area; white arrows, CD44 + cells; scale bar, 50 mm. (e) Comparison of Arpin and Arp2/3 expressions in BM cells after JNK blockade in vitro . (f) Analysis of the Arpin, Arp2, and Arp3 mRNA expression in sorted CD44 + cells. On day 3 postoperatively, cells in PB were sorted by FACS on CD44. RT-PCR was performed to evaluate Arpin, Arp2, and Arp3 mRNA expression. The quantification data are shown as a bar graph ( n = 3). ∗∗ P < 0.01.

Article Snippet: Arp2/3 Ab , Western blot , 0.1 μ g/ml , 24 h , CST, USA.

Techniques: Migration, Construct, Expressing, Western Blot, In Vivo, In Vitro, Reverse Transcription Polymerase Chain Reaction