armored hcv rna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher armored hcv rna
    Age related decline in prevalence of hepatitis C antibody in infants who had <t>anti-HCV</t> detected in their initial blood sample according to whether their mothers were positive for both <t>HCV-RNA</t> and anti-HCV or only had anti-HCV.
    Armored Hcv Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/armored hcv rna/product/Thermo Fisher
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    armored hcv rna - by Bioz Stars, 2020-05
    85/100 stars

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    1) Product Images from "Prospective Cohort Study of Mother-to-Infant Infection and Clearance of Hepatitis C in Rural Egyptian Villages"

    Article Title: Prospective Cohort Study of Mother-to-Infant Infection and Clearance of Hepatitis C in Rural Egyptian Villages

    Journal: Journal of medical virology

    doi: 10.1002/jmv.21480

    Age related decline in prevalence of hepatitis C antibody in infants who had anti-HCV detected in their initial blood sample according to whether their mothers were positive for both HCV-RNA and anti-HCV or only had anti-HCV.
    Figure Legend Snippet: Age related decline in prevalence of hepatitis C antibody in infants who had anti-HCV detected in their initial blood sample according to whether their mothers were positive for both HCV-RNA and anti-HCV or only had anti-HCV.

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    Article Title: Prospective Cohort Study of Mother-to-Infant Infection and Clearance of Hepatitis C in Rural Egyptian Villages
    Article Snippet: .. Quantitative controls consisting of known copy numbers generated from Armored HCV RNA (Ambion, Austin, TX) were run with each reaction. ..

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    Thermo Fisher genomic hcv rna
    Disruption in the 5′UTR nt95–110:NS5B nt8528–8543 duplex reduces intracellular <t>HCV</t> <t>RNA</t> levels. ( A ) Huh-7.5 cells transfected with HCV genomic RNA (WT) or HCV genomic RNA containing mutations at 5′UTR nt 95–110 and NS5B nt 8528–8543 express NS5B antigen 24 h post-transfection. Scale bar = 50 µm. ( B ) Plots represent the average number of HCV RNA genome copies ± SE in 1 μg of cellular RNA at 48 h post-transfection. p -values ≤ 0.05 (*) or ≤ 0.01 (**) were determined by the Student’s t -test and represent four or more independent experiments.
    Genomic Hcv Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/genomic hcv rna/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    genomic hcv rna - by Bioz Stars, 2020-05
    85/100 stars
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    Disruption in the 5′UTR nt95–110:NS5B nt8528–8543 duplex reduces intracellular HCV RNA levels. ( A ) Huh-7.5 cells transfected with HCV genomic RNA (WT) or HCV genomic RNA containing mutations at 5′UTR nt 95–110 and NS5B nt 8528–8543 express NS5B antigen 24 h post-transfection. Scale bar = 50 µm. ( B ) Plots represent the average number of HCV RNA genome copies ± SE in 1 μg of cellular RNA at 48 h post-transfection. p -values ≤ 0.05 (*) or ≤ 0.01 (**) were determined by the Student’s t -test and represent four or more independent experiments.

    Journal: Viruses

    Article Title: Genomic-Scale Interaction Involving Complementary Sequences in the Hepatitis C Virus 5′UTR Domain IIa and the RNA-Dependent RNA Polymerase Coding Region Promotes Efficient Virus Replication

    doi: 10.3390/v11010017

    Figure Lengend Snippet: Disruption in the 5′UTR nt95–110:NS5B nt8528–8543 duplex reduces intracellular HCV RNA levels. ( A ) Huh-7.5 cells transfected with HCV genomic RNA (WT) or HCV genomic RNA containing mutations at 5′UTR nt 95–110 and NS5B nt 8528–8543 express NS5B antigen 24 h post-transfection. Scale bar = 50 µm. ( B ) Plots represent the average number of HCV RNA genome copies ± SE in 1 μg of cellular RNA at 48 h post-transfection. p -values ≤ 0.05 (*) or ≤ 0.01 (**) were determined by the Student’s t -test and represent four or more independent experiments.

    Article Snippet: Genomic HCV RNA was synthesized using the MEGAscript T7 kit (Thermo Fisher Scientific) with 1 µg of Xba I-linearized, mung bean nuclease-treated pER-1b or pER-1b containing 5′UTR or NS5B mutations (Thermo Fisher Scientific).

    Techniques: Transfection

    Disruption in the 5′UTR nt 95–110:NS5B nt 8528–8543 duplex reduced progeny virus titers. Huh-7.5 cells were infected with HCV parental virus (WT) or HCV containing mutations at 5′UTR nt 95–110 and NS5B nt 8528–8543. Plots represent the average number of infectious virus titers as focus-forming units (FFU)/mL or HCV RNA genome copies ± SE in 1 μg of cellular RNA taken at 48 h post-infection. p -values ≤ 0.05 (*) or ≤ 0.01 (**) were determined by the Student’s t -test and represent six independent experiments.

    Journal: Viruses

    Article Title: Genomic-Scale Interaction Involving Complementary Sequences in the Hepatitis C Virus 5′UTR Domain IIa and the RNA-Dependent RNA Polymerase Coding Region Promotes Efficient Virus Replication

    doi: 10.3390/v11010017

    Figure Lengend Snippet: Disruption in the 5′UTR nt 95–110:NS5B nt 8528–8543 duplex reduced progeny virus titers. Huh-7.5 cells were infected with HCV parental virus (WT) or HCV containing mutations at 5′UTR nt 95–110 and NS5B nt 8528–8543. Plots represent the average number of infectious virus titers as focus-forming units (FFU)/mL or HCV RNA genome copies ± SE in 1 μg of cellular RNA taken at 48 h post-infection. p -values ≤ 0.05 (*) or ≤ 0.01 (**) were determined by the Student’s t -test and represent six independent experiments.

    Article Snippet: Genomic HCV RNA was synthesized using the MEGAscript T7 kit (Thermo Fisher Scientific) with 1 µg of Xba I-linearized, mung bean nuclease-treated pER-1b or pER-1b containing 5′UTR or NS5B mutations (Thermo Fisher Scientific).

    Techniques: Infection