are luc activity  (Millipore)


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    Name:
    Luciferase
    Description:
    Luciferase is a class of enzymes naturally found in organisms like protists fungi insects bacteria which belong to phylogenic kingdom It is used as a molecular marker for biological imaging as it specifically cleaves luciferin substrate which results in emitting light
    Catalog Number:
    10411523001
    Price:
    None
    Applications:
    Determination of ATP in ATP-generating or ATP-consuming reactions. Determination of the activity of enzymes such as creatine kinase and ATPases. Under optimal conditions, 1 photon is released per molecule ATP in the bioluminescence assay.One mg is sufficient for approximately 5,000 ATP determinations in the concentration range of 0.1 nM to 1 muM.
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    Structured Review

    Millipore are luc activity
    Phf2 and ChREBP decrease oxidative stress through Nrf2 activation. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in fed state. a Representative Western blot analysis of Nrf2 protein content and Nrf2-regulated genes ( n = 10 mice per group). b – d Nrf2 was inhibited in cultured hepatocytes overexpressing Phf2. b Western blot analysis of proteins involved in oxidative stress defenses ( n = 3). GSH and NADPH contents ( c ) in addition to ROS levels and hepatocyte apoptosis ( d ) were determined after incubation with 480 μM palmitate for 24 h ( n = 3). e UCSC genome browser image illustrating normalized tag counts for Phf2, ChREBP, H3K9me2, H3K4me3, and RNA polII at the Nrf2 promoter. ( f , g ) Phf2 was inhibited in cultured hepatocytes. ChIP for H3K9me2, ChREBP, and RNA polII, in addition to chromatin accessibility at the Nrf2 promoter and Nrf2 activity on the <t>ARE-luc</t> construct shown ( n = 3). h – j ChREBP expression was inhibited in cultured hepatocytes overexpressing Phf2. h Representative western blot analysis showing the contribution of ChREBP to the regulation of Nrf2 and Nrf2-regulated gene expression. i Nrf2 activity on the ARE-luc construct shown. j Relative ROS levels and measurement of hepatocyte apoptosis were determined after incubation with 480 μM palmitate for 24 h ( n = 3). All error bars represent mean ± SEM. Statistical analyses were made using Anova, followed by Bonferonni’s test. * P
    Luciferase is a class of enzymes naturally found in organisms like protists fungi insects bacteria which belong to phylogenic kingdom It is used as a molecular marker for biological imaging as it specifically cleaves luciferin substrate which results in emitting light
    https://www.bioz.com/result/are luc activity/product/Millipore
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    are luc activity - by Bioz Stars, 2020-08
    99/100 stars

    Images

    1) Product Images from "The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity"

    Article Title: The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity

    Journal: Nature Communications

    doi: 10.1038/s41467-018-04361-y

    Phf2 and ChREBP decrease oxidative stress through Nrf2 activation. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in fed state. a Representative Western blot analysis of Nrf2 protein content and Nrf2-regulated genes ( n = 10 mice per group). b – d Nrf2 was inhibited in cultured hepatocytes overexpressing Phf2. b Western blot analysis of proteins involved in oxidative stress defenses ( n = 3). GSH and NADPH contents ( c ) in addition to ROS levels and hepatocyte apoptosis ( d ) were determined after incubation with 480 μM palmitate for 24 h ( n = 3). e UCSC genome browser image illustrating normalized tag counts for Phf2, ChREBP, H3K9me2, H3K4me3, and RNA polII at the Nrf2 promoter. ( f , g ) Phf2 was inhibited in cultured hepatocytes. ChIP for H3K9me2, ChREBP, and RNA polII, in addition to chromatin accessibility at the Nrf2 promoter and Nrf2 activity on the ARE-luc construct shown ( n = 3). h – j ChREBP expression was inhibited in cultured hepatocytes overexpressing Phf2. h Representative western blot analysis showing the contribution of ChREBP to the regulation of Nrf2 and Nrf2-regulated gene expression. i Nrf2 activity on the ARE-luc construct shown. j Relative ROS levels and measurement of hepatocyte apoptosis were determined after incubation with 480 μM palmitate for 24 h ( n = 3). All error bars represent mean ± SEM. Statistical analyses were made using Anova, followed by Bonferonni’s test. * P
    Figure Legend Snippet: Phf2 and ChREBP decrease oxidative stress through Nrf2 activation. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in fed state. a Representative Western blot analysis of Nrf2 protein content and Nrf2-regulated genes ( n = 10 mice per group). b – d Nrf2 was inhibited in cultured hepatocytes overexpressing Phf2. b Western blot analysis of proteins involved in oxidative stress defenses ( n = 3). GSH and NADPH contents ( c ) in addition to ROS levels and hepatocyte apoptosis ( d ) were determined after incubation with 480 μM palmitate for 24 h ( n = 3). e UCSC genome browser image illustrating normalized tag counts for Phf2, ChREBP, H3K9me2, H3K4me3, and RNA polII at the Nrf2 promoter. ( f , g ) Phf2 was inhibited in cultured hepatocytes. ChIP for H3K9me2, ChREBP, and RNA polII, in addition to chromatin accessibility at the Nrf2 promoter and Nrf2 activity on the ARE-luc construct shown ( n = 3). h – j ChREBP expression was inhibited in cultured hepatocytes overexpressing Phf2. h Representative western blot analysis showing the contribution of ChREBP to the regulation of Nrf2 and Nrf2-regulated gene expression. i Nrf2 activity on the ARE-luc construct shown. j Relative ROS levels and measurement of hepatocyte apoptosis were determined after incubation with 480 μM palmitate for 24 h ( n = 3). All error bars represent mean ± SEM. Statistical analyses were made using Anova, followed by Bonferonni’s test. * P

    Techniques Used: Activation Assay, Mouse Assay, Injection, Western Blot, Cell Culture, Incubation, Chromatin Immunoprecipitation, Activity Assay, Construct, Expressing

    2) Product Images from "The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity"

    Article Title: The histone demethylase Phf2 acts as a molecular checkpoint to prevent NAFLD progression during obesity

    Journal: Nature Communications

    doi: 10.1038/s41467-018-04361-y

    Phf2 and ChREBP decrease oxidative stress through Nrf2 activation. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in fed state. a Representative Western blot analysis of Nrf2 protein content and Nrf2-regulated genes ( n = 10 mice per group). b – d Nrf2 was inhibited in cultured hepatocytes overexpressing Phf2. b Western blot analysis of proteins involved in oxidative stress defenses ( n = 3). GSH and NADPH contents ( c ) in addition to ROS levels and hepatocyte apoptosis ( d ) were determined after incubation with 480 μM palmitate for 24 h ( n = 3). e UCSC genome browser image illustrating normalized tag counts for Phf2, ChREBP, H3K9me2, H3K4me3, and RNA polII at the Nrf2 promoter. ( f , g ) Phf2 was inhibited in cultured hepatocytes. ChIP for H3K9me2, ChREBP, and RNA polII, in addition to chromatin accessibility at the Nrf2 promoter and Nrf2 activity on the ARE-luc construct shown ( n = 3). h – j ChREBP expression was inhibited in cultured hepatocytes overexpressing Phf2. h Representative western blot analysis showing the contribution of ChREBP to the regulation of Nrf2 and Nrf2-regulated gene expression. i Nrf2 activity on the ARE-luc construct shown. j Relative ROS levels and measurement of hepatocyte apoptosis were determined after incubation with 480 μM palmitate for 24 h ( n = 3). All error bars represent mean ± SEM. Statistical analyses were made using Anova, followed by Bonferonni’s test. * P
    Figure Legend Snippet: Phf2 and ChREBP decrease oxidative stress through Nrf2 activation. Mice, injected with either GFP or Phf2 overexpressing adenovirus, were studied 3 weeks later in fed state. a Representative Western blot analysis of Nrf2 protein content and Nrf2-regulated genes ( n = 10 mice per group). b – d Nrf2 was inhibited in cultured hepatocytes overexpressing Phf2. b Western blot analysis of proteins involved in oxidative stress defenses ( n = 3). GSH and NADPH contents ( c ) in addition to ROS levels and hepatocyte apoptosis ( d ) were determined after incubation with 480 μM palmitate for 24 h ( n = 3). e UCSC genome browser image illustrating normalized tag counts for Phf2, ChREBP, H3K9me2, H3K4me3, and RNA polII at the Nrf2 promoter. ( f , g ) Phf2 was inhibited in cultured hepatocytes. ChIP for H3K9me2, ChREBP, and RNA polII, in addition to chromatin accessibility at the Nrf2 promoter and Nrf2 activity on the ARE-luc construct shown ( n = 3). h – j ChREBP expression was inhibited in cultured hepatocytes overexpressing Phf2. h Representative western blot analysis showing the contribution of ChREBP to the regulation of Nrf2 and Nrf2-regulated gene expression. i Nrf2 activity on the ARE-luc construct shown. j Relative ROS levels and measurement of hepatocyte apoptosis were determined after incubation with 480 μM palmitate for 24 h ( n = 3). All error bars represent mean ± SEM. Statistical analyses were made using Anova, followed by Bonferonni’s test. * P

    Techniques Used: Activation Assay, Mouse Assay, Injection, Western Blot, Cell Culture, Incubation, Chromatin Immunoprecipitation, Activity Assay, Construct, Expressing

    Related Articles

    Clone Assay:

    Article Title: Cloning and characterization of rabbit Rgs4 promoter in gut smooth muscle
    Article Snippet: .. This fragment (designed as Rgs4-P1) was inserted by T-A cloning into the lineated promoter-less pMlu3 AccepTor vector at EcoRV of multiple cloning sties upstream of the secreted renilla luciferase reporter (EMD-Bioscience/Novagen). .. The direction and sequence of the insert were validated by sequencing with upstream and downstream vector primers ( ).

    Transfection:

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Article Title: miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR
    Article Snippet: .. Briefly, MCF-7 and MDA-MB-231 cells transfected with Luc-NRP-1-wt or Luc-NRP-1-mut were lysed with 25 mM Tris-HCl buffer (pH 7.5) and 100 U/mL RNase inhibitor (Sigma-Aldrich Co.), and then incubated with protein-A sepharose beads pre-coated with 3 µg anti-Ago2 antibody or control rabbit IgG for 1.5 hours at 4°C. .. The RNA–protein complexes were pulled down by protein A/G agarose beads, and RNA was extracted with TransZol Up, and miR-376a level was measured by qRT-PCR.

    Luciferase:

    Article Title: Long non-coding RNA MEG3 inhibits the proliferation and metastasis of oral squamous cell carcinoma by regulating the WNT/β-catenin signaling pathway
    Article Snippet: .. Cell Counting Kit-8 (CCK-8) (Dojindo, Kumamoto, Japan); Lipofectamine 3000 (Invitrogen Life Technologies, New York, NY, USA); Annexin V-FITC cell apoptosis detection kit (eBioscience, San Diego, CA, USA); 5-aza-2′-deoxycytidine (5-aza-CdR; Sigma, New York, NY, USA); TOP/FOP-Flash luciferase reporter vector (Millipore, Billerica, MA, USA); Dual-Luciferase assay kit (Promega, Madison, WI, USA); primers (Sangon, Shanghai, China). .. Cell culture and transfection SCC15 and Cal27 cells were cultured in DMEM (10% FBS) until the cells adhered; pcDNA3.1-MEG3 overexpression vector and the si-MEG3 interference vector and its corresponding control vector pcDNA3.1-Ctrl were constructed, si-Ctrl were transfected into cells using liposomes.

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Article Title: Cloning and characterization of rabbit Rgs4 promoter in gut smooth muscle
    Article Snippet: .. This fragment (designed as Rgs4-P1) was inserted by T-A cloning into the lineated promoter-less pMlu3 AccepTor vector at EcoRV of multiple cloning sties upstream of the secreted renilla luciferase reporter (EMD-Bioscience/Novagen). .. The direction and sequence of the insert were validated by sequencing with upstream and downstream vector primers ( ).

    Stable Transfection:

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Multiple Displacement Amplification:

    Article Title: miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR
    Article Snippet: .. Briefly, MCF-7 and MDA-MB-231 cells transfected with Luc-NRP-1-wt or Luc-NRP-1-mut were lysed with 25 mM Tris-HCl buffer (pH 7.5) and 100 U/mL RNase inhibitor (Sigma-Aldrich Co.), and then incubated with protein-A sepharose beads pre-coated with 3 µg anti-Ago2 antibody or control rabbit IgG for 1.5 hours at 4°C. .. The RNA–protein complexes were pulled down by protein A/G agarose beads, and RNA was extracted with TransZol Up, and miR-376a level was measured by qRT-PCR.

    CCK-8 Assay:

    Article Title: Long non-coding RNA MEG3 inhibits the proliferation and metastasis of oral squamous cell carcinoma by regulating the WNT/β-catenin signaling pathway
    Article Snippet: .. Cell Counting Kit-8 (CCK-8) (Dojindo, Kumamoto, Japan); Lipofectamine 3000 (Invitrogen Life Technologies, New York, NY, USA); Annexin V-FITC cell apoptosis detection kit (eBioscience, San Diego, CA, USA); 5-aza-2′-deoxycytidine (5-aza-CdR; Sigma, New York, NY, USA); TOP/FOP-Flash luciferase reporter vector (Millipore, Billerica, MA, USA); Dual-Luciferase assay kit (Promega, Madison, WI, USA); primers (Sangon, Shanghai, China). .. Cell culture and transfection SCC15 and Cal27 cells were cultured in DMEM (10% FBS) until the cells adhered; pcDNA3.1-MEG3 overexpression vector and the si-MEG3 interference vector and its corresponding control vector pcDNA3.1-Ctrl were constructed, si-Ctrl were transfected into cells using liposomes.

    Blocking Assay:

    Article Title: A novel bioassay for anti-thyrotrophin receptor autoantibodies detects both thyroid-blocking and stimulating activity
    Article Snippet: .. Chimeric CHO-Luc or wild-type CHO-Luc cells were induced with bovine TSH (bTSH) (Sigma Aldrich) mixed with blocking monoclonal antibody (mAb) K1-70, stimulating mAb, M22 (RSR, Cardiff, UK) or human sera. ..

    Incubation:

    Article Title: miR-376a inhibits breast cancer cell progression by targeting neuropilin-1 NR
    Article Snippet: .. Briefly, MCF-7 and MDA-MB-231 cells transfected with Luc-NRP-1-wt or Luc-NRP-1-mut were lysed with 25 mM Tris-HCl buffer (pH 7.5) and 100 U/mL RNase inhibitor (Sigma-Aldrich Co.), and then incubated with protein-A sepharose beads pre-coated with 3 µg anti-Ago2 antibody or control rabbit IgG for 1.5 hours at 4°C. .. The RNA–protein complexes were pulled down by protein A/G agarose beads, and RNA was extracted with TransZol Up, and miR-376a level was measured by qRT-PCR.

    Selection:

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Plasmid Preparation:

    Article Title: Long non-coding RNA MEG3 inhibits the proliferation and metastasis of oral squamous cell carcinoma by regulating the WNT/β-catenin signaling pathway
    Article Snippet: .. Cell Counting Kit-8 (CCK-8) (Dojindo, Kumamoto, Japan); Lipofectamine 3000 (Invitrogen Life Technologies, New York, NY, USA); Annexin V-FITC cell apoptosis detection kit (eBioscience, San Diego, CA, USA); 5-aza-2′-deoxycytidine (5-aza-CdR; Sigma, New York, NY, USA); TOP/FOP-Flash luciferase reporter vector (Millipore, Billerica, MA, USA); Dual-Luciferase assay kit (Promega, Madison, WI, USA); primers (Sangon, Shanghai, China). .. Cell culture and transfection SCC15 and Cal27 cells were cultured in DMEM (10% FBS) until the cells adhered; pcDNA3.1-MEG3 overexpression vector and the si-MEG3 interference vector and its corresponding control vector pcDNA3.1-Ctrl were constructed, si-Ctrl were transfected into cells using liposomes.

    Article Title: Viral Source-Independent High Susceptibility of Dendritic Cells to Human T-Cell Leukemia Virus Type 1 Infection Compared to That of T Lymphocytes
    Article Snippet: .. Jurkat-LTR-Luc cells, stably transfected with a plasmid encoding luciferase under the control of the HTLV-1 long terminal repeat (LTR) promoter, were maintained under hygromycin (450 μg/ml, Sigma) selection. .. Jurkat-LTR-GFP cells were maintained under G418 (750 μg/ml; Gibco Life Technologies) selection.

    Article Title: The WHHERE coactivator complex is required for retinoic acid-dependent regulation of embryonic symmetry
    Article Snippet: .. Vector construction and baculovirus generation To assay RA signaling in NIH3T3 cells, we used the RA reporter RARE-Luciferase and mouse Rere-HA subcloned into pMyc-CMV (Sigma) described in ref. . .. The mouse full-length Wdr5 , Hdac1 , Hdac2 , and Rarα cDNAs were subcloned into pFlag-CMV (Sigma).

    Article Title: Cloning and characterization of rabbit Rgs4 promoter in gut smooth muscle
    Article Snippet: .. This fragment (designed as Rgs4-P1) was inserted by T-A cloning into the lineated promoter-less pMlu3 AccepTor vector at EcoRV of multiple cloning sties upstream of the secreted renilla luciferase reporter (EMD-Bioscience/Novagen). .. The direction and sequence of the insert were validated by sequencing with upstream and downstream vector primers ( ).

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  • 99
    Millipore 21nt sirna luc
    The 21-nt <t>siRNA</t> requires a cell-permeating entity for internalization by LECs. mLEC cultures were exposed to fluorescein-conjugated <t>21nt-siRNA-</t> <t>Luc</t> (Fl-si Luc ), followed by time-lapse confocal microscopy (left column, Nomarski; right column, green channel).
    21nt Sirna Luc, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/21nt sirna luc/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    21nt sirna luc - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    99
    Millipore renilla luciferase reporter
    Promoter activity of rabbit Rgs4 5′-flanked region (1.4 kb) in various cells. Cultured cells were cotransfected with promoter-less pMlu3 vector or Rgs4 promoter vector carrying secreted <t>renilla</t> luciferase and pGL4-CMV vector carrying firefly luciferase.
    Renilla Luciferase Reporter, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/renilla luciferase reporter/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    renilla luciferase reporter - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    The 21-nt siRNA requires a cell-permeating entity for internalization by LECs. mLEC cultures were exposed to fluorescein-conjugated 21nt-siRNA- Luc (Fl-si Luc ), followed by time-lapse confocal microscopy (left column, Nomarski; right column, green channel).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth

    doi: 10.1073/pnas.0812317106

    Figure Lengend Snippet: The 21-nt siRNA requires a cell-permeating entity for internalization by LECs. mLEC cultures were exposed to fluorescein-conjugated 21nt-siRNA- Luc (Fl-si Luc ), followed by time-lapse confocal microscopy (left column, Nomarski; right column, green channel).

    Article Snippet: Lysates from sutured mouse corneas treated with either PBS or 21nt-siRNA- Luc (300 pmol) were used to quantify CXCL10 and IL-12 protein levels by fluorescent bead assay technology (Beadlyte; Millipore) per the manufacturer's instructions.

    Techniques: Confocal Microscopy

    Promoter activity of rabbit Rgs4 5′-flanked region (1.4 kb) in various cells. Cultured cells were cotransfected with promoter-less pMlu3 vector or Rgs4 promoter vector carrying secreted renilla luciferase and pGL4-CMV vector carrying firefly luciferase.

    Journal: Gene

    Article Title: Cloning and characterization of rabbit Rgs4 promoter in gut smooth muscle

    doi: 10.1016/j.gene.2009.11.010

    Figure Lengend Snippet: Promoter activity of rabbit Rgs4 5′-flanked region (1.4 kb) in various cells. Cultured cells were cotransfected with promoter-less pMlu3 vector or Rgs4 promoter vector carrying secreted renilla luciferase and pGL4-CMV vector carrying firefly luciferase.

    Article Snippet: This fragment (designed as Rgs4-P1) was inserted by T-A cloning into the lineated promoter-less pMlu3 AccepTor vector at EcoRV of multiple cloning sties upstream of the secreted renilla luciferase reporter (EMD-Bioscience/Novagen).

    Techniques: Activity Assay, Cell Culture, Plasmid Preparation, Luciferase