Aqp7, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
Average 91 stars, based on 4 article reviews
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1) Product Images from "Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes"
Article Title: Lipopolysaccharide Modifies Glycerol Permeability and Metabolism in 3T3-L1 Adipocytes
Journal: International Journal of Molecular Sciences
Figure Legend Snippet: Relative contributions of AQP7 and AQP3 to glycerol permeability in plasma membrane vesicles from UDCs, CTL-DCs and LPS-DCs. Ki values were determined by stopped-flow light scattering analysis of plasma membrane vesicles prepared from UDCs, untreated DCs (CTL) and LPS-treated DCs (LPS) in the absence or presence of 0.3 mM HgCl 2 or 1.0 mM CuSO 4 as described in Materials and Methods. The results are the mean ± S.E.M. of the Ki values from 7 to 12 independent vesicles preparations. Data were analyzed using One-way ANOVA and Bonferroni post-hoc t -test. For HgCl 2 : °° p
Techniques Used: Permeability
Figure Legend Snippet: Model for the role of aquaglyceroporins in the regulation of lipogenesis and lipolysis in mouse adipocytes. ( A ) Lipogenesis: insulin is secreted in response to an increase of glycaemia consecutive to feeding. Glucose metabolites, glycerol-3 phosphate (G-3-P) and free fatty acids are used to produce TAGs that will be stored. Both AQP3 and AQP7 are internalized. AQP9 is constitutively expressed at the plasma membrane; ( B ) Lipolysis: upon glucagon secretion in response to fasting or cAMP-mediated response (exercise, catecholamine secretion), TAGs are hydrolyzed to glycerol and free fatty acids, both AQP7 and AQP3 traffic to the plasma membrane enabling increased glycerol efflux. AQP9 may also participate to glycerol efflux during lipolysis. Arrows inside AQP channels represent the direction of glycerol flow; ( C ) Under inflammatory conditions mimicking obesity, LPS is hypothesized to induce AQP3 and AQP9 internalization, thereby reducing plasma membrane glycerol permeability and intracellular TAGs accumulation. Abbreviations: TAGs, triacylglycerols; G-3-P, glycerol-3-phosphate; FFA, free fatty acids; H, catecholamine or glucagon; AC, adenylyl cyclase; ATP, adenosine triphosphate; cAMP, cyclic adenosine monophosphate; PKA, protein kinase A; HSL, hormone sensitive lipase; LPS, lipopolysaccharide; TLR4, Toll-like receptor 4; AQP, aquaporin.
Techniques Used: Permeability
Figure Legend Snippet: AQP3, AQP7 and AQP9 protein expression in UDCs, CTL-DCs and LPS-DCs. AQP3, AQP7 and AQP9 protein expression levels were determined in UDCs, untreated DCs (CTL-DCs) or LPS-treated DCs (1 μg LPS/mL) by Western blotting analysis. ( A ) AQP7 (30 kDa), AQP3 (30 kDa), AQP9 (30 kDa) and β-actin (42 kDa) were detected using specific antibodies as described in Materials and Methods. Western blot was representative of 3 independent experiments; ( B ) The relative expression of each AQP was determined by densitometry analysis of the Western blots as described in Materials and Methods. Ratio between AQP band volume and β-actin band volume were calculated, the relative expression of each AQP in LPS-DCs is expressed in fold of the ratio calculated for CTL-DCs (set to 1) and is the mean ± standard error of the mean (S.E.M.) of 3 independents experiments. Statistical analysis was performed using the t -test for the unique sample.
Techniques Used: Expressing, Western Blot
2) Product Images from "Empagliflozin Contributes to Polyuria via Regulation of Sodium Transporters and Water Channels in Diabetic Rat Kidneys"
Article Title: Empagliflozin Contributes to Polyuria via Regulation of Sodium Transporters and Water Channels in Diabetic Rat Kidneys
Journal: Frontiers in Physiology
Figure Legend Snippet: Empagliflozin causes no significant changes to AQP3 and AQP1 but tends to increase AQP7 level in diabetic rat kidneys. (A) Representative renal sections immunostained with anti-AQP3. (B) Quantitative analysis of results for AQP3 shows no significant change among groups. Magnification, ×200. (C) Representative immunoblot reacting with anti-NCC, anti-α-ENaC and anti- γ-ENaC. (D) Densitometric analysis shows that the expression of AQP3 protein significantly increased in all OLETF rats. Lixisenatide treatment further increased the expression of AQP3. ∗ P
Techniques Used: Expressing