aqp4  (Alomone Labs)


Bioz Verified Symbol Alomone Labs is a verified supplier
Bioz Manufacturer Symbol Alomone Labs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Alomone Labs aqp4
    Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with <t>anti-AQP4,</t> anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)
    Aqp4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aqp4/product/Alomone Labs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    aqp4 - by Bioz Stars, 2022-09
    95/100 stars

    Images

    1) Product Images from "Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer’s disease mouse model"

    Article Title: Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer’s disease mouse model

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-1264-8

    Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)
    Figure Legend Snippet: Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)

    Techniques Used: Immunofluorescence, Staining, Generated, Immunostaining

    Western blot analysis and quantification of astroglial marker proteins Aqp4, GS, Aldh1l1 and GFAP. Western blot analysis ( a ) was performed from four independently generated iAstro lines for each genotype (WT-iAstro#2, #3, #5 and #6, and 3Tg-iAstro#2, #3, #4 and #6). Each point represents mean ± SEM of 3 independent experiments. Actin was used as loading control. ANOVA followed by Tukey’s post-hoc test was used for statistical analysis. For Aqp4 ( b ) and GS ( c ) there were no significant differences. For Aldh1l1 ( d ) and GFAP ( e ) the differences were significant for iAstro lines vs primary astrocytes: ** p
    Figure Legend Snippet: Western blot analysis and quantification of astroglial marker proteins Aqp4, GS, Aldh1l1 and GFAP. Western blot analysis ( a ) was performed from four independently generated iAstro lines for each genotype (WT-iAstro#2, #3, #5 and #6, and 3Tg-iAstro#2, #3, #4 and #6). Each point represents mean ± SEM of 3 independent experiments. Actin was used as loading control. ANOVA followed by Tukey’s post-hoc test was used for statistical analysis. For Aqp4 ( b ) and GS ( c ) there were no significant differences. For Aldh1l1 ( d ) and GFAP ( e ) the differences were significant for iAstro lines vs primary astrocytes: ** p

    Techniques Used: Western Blot, Marker, Generated

    2) Product Images from "Functional Implication of Dp71 in Osmoregulation and Vascular Permeability of the Retina"

    Article Title: Functional Implication of Dp71 in Osmoregulation and Vascular Permeability of the Retina

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0007329

    Experimental retinal detachment (RD) changed the expression and immunolocalization of GFAP, Kir4.1, AQP4, Dp71, Utrophin and β-dystroglycan. The slices were derived from control and detached retina of C57BL/6 mice 24 h after surgery. A,G: Retinal sections were probed with antibodies against GFAP (green) and for nuclei (Dapi, blue). Note the upregulation of GFAP after RD. B,H: Sections were also stained with antibodies against Kir4.1 (green). RD induced a mislocation of Kir4.1 along Müller cells (filled arrowhead) while the staining at the ILM (open arrowhead) and around blood vessels (arrow) remain unchanged. C,I: AQP4 staining (green) at the ILM (open arrowhead), around blood vessels (arrow) and at the OPL (filled arrowhead) was reduced in detached retina. D,J: Retinal sections were probed with a pan specific antibody against all forms of dystrophins (green). Dystrophin-Dp71 staining is localized at the ILM (open arrowhead) and around blood vessels (arrow), as previously reported [14] ( Fig. S2 ). RD induced a reduction of Dp71 staining. E,K: 24 h after surgery Immunoreactivity for Utrophin (green) at the ILM (open arrowhead) was upregulated after RD. In control retina utrophin was localized at the ILM, around blood vessels and in the OPL. F,L: Sections were stained with antibodies against β-dystroglycan (β-DG). In control retina, β-DG is localized at the ILM (open arrowhead), around blood vessels (arrow) and at the OPL (filled arrowhead). RD induced a reduction of the staining in the ILM while the staining at the OPL (filled arrowhead) and around blood vessels (arrow) remain unchanged. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; ILM, inner limiting membrane. Scale bar = 30 µm.
    Figure Legend Snippet: Experimental retinal detachment (RD) changed the expression and immunolocalization of GFAP, Kir4.1, AQP4, Dp71, Utrophin and β-dystroglycan. The slices were derived from control and detached retina of C57BL/6 mice 24 h after surgery. A,G: Retinal sections were probed with antibodies against GFAP (green) and for nuclei (Dapi, blue). Note the upregulation of GFAP after RD. B,H: Sections were also stained with antibodies against Kir4.1 (green). RD induced a mislocation of Kir4.1 along Müller cells (filled arrowhead) while the staining at the ILM (open arrowhead) and around blood vessels (arrow) remain unchanged. C,I: AQP4 staining (green) at the ILM (open arrowhead), around blood vessels (arrow) and at the OPL (filled arrowhead) was reduced in detached retina. D,J: Retinal sections were probed with a pan specific antibody against all forms of dystrophins (green). Dystrophin-Dp71 staining is localized at the ILM (open arrowhead) and around blood vessels (arrow), as previously reported [14] ( Fig. S2 ). RD induced a reduction of Dp71 staining. E,K: 24 h after surgery Immunoreactivity for Utrophin (green) at the ILM (open arrowhead) was upregulated after RD. In control retina utrophin was localized at the ILM, around blood vessels and in the OPL. F,L: Sections were stained with antibodies against β-dystroglycan (β-DG). In control retina, β-DG is localized at the ILM (open arrowhead), around blood vessels (arrow) and at the OPL (filled arrowhead). RD induced a reduction of the staining in the ILM while the staining at the OPL (filled arrowhead) and around blood vessels (arrow) remain unchanged. ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; ILM, inner limiting membrane. Scale bar = 30 µm.

    Techniques Used: Expressing, Derivative Assay, Mouse Assay, Staining

    Relative retinal expression level of GFAP, Kir4.1, AQP4, Utrophin and β-dystroglycan after retinal detachment. A: 24 h after surgery, proteins of control and detached retina from C57BL/6 mice were extracted and Western blotting was performed. Representative photograph of immunoblots reacted with anti-GFAP, anti-Kir4.1, anti-AQP4, anti utrophins, anti β-dystroglycan (β-DG) and β-Actin antibodies. Numbers on the left refer to the relative electrophoretic mobility of prestained molecular mass standards in kiloDaltons. B: The relative protein level is expressed in arbitrary units. Each value represents the ratio of the specific band stain intensity normalized to β-Actin expression (TotalLab TL120, Nonlinear Inc, Durham NC, USA). In detached retina, GFAP expression level was significantly upregulated while AQP4 expression was downregulated. There was no significant difference in Kir4.1, utrophin and β-DG protein expression level after retinal detachment. All experiments were repeated at least three times, and the bars represent means + SE for triplicate data points; n = 4. *p
    Figure Legend Snippet: Relative retinal expression level of GFAP, Kir4.1, AQP4, Utrophin and β-dystroglycan after retinal detachment. A: 24 h after surgery, proteins of control and detached retina from C57BL/6 mice were extracted and Western blotting was performed. Representative photograph of immunoblots reacted with anti-GFAP, anti-Kir4.1, anti-AQP4, anti utrophins, anti β-dystroglycan (β-DG) and β-Actin antibodies. Numbers on the left refer to the relative electrophoretic mobility of prestained molecular mass standards in kiloDaltons. B: The relative protein level is expressed in arbitrary units. Each value represents the ratio of the specific band stain intensity normalized to β-Actin expression (TotalLab TL120, Nonlinear Inc, Durham NC, USA). In detached retina, GFAP expression level was significantly upregulated while AQP4 expression was downregulated. There was no significant difference in Kir4.1, utrophin and β-DG protein expression level after retinal detachment. All experiments were repeated at least three times, and the bars represent means + SE for triplicate data points; n = 4. *p

    Techniques Used: Expressing, Mouse Assay, Western Blot, Staining

    3) Product Images from "Anti-VEGF therapy prevents Müller intracellular edema by decreasing VEGF-A in diabetic retinopathy"

    Article Title: Anti-VEGF therapy prevents Müller intracellular edema by decreasing VEGF-A in diabetic retinopathy

    Journal: Eye and Vision

    doi: 10.1186/s40662-021-00237-3

    Changes in mRNA and protein levels of Kir4.1 and AQP4 in glyoxal-treated rMC-1 cells with or without ranibizumab. The ( a ) mRNA and ( b ) protein level of Kir4.1 in glyoxal-treated rMC-1 cells with or without ranibizumab. c Immunofluorescence of Kir4.1 in rMC-1 cells. The ( d ) mRNA and ( e ) protein level of AQP4 in glyoxal-treated rMC-1 cells with or without ranibizumab. f Immunofluorescence of AQP4 in rMC-1 cells. Data are expressed as mean ± SE (n = 8 in [a, d], n = 4 in [b, e], * P
    Figure Legend Snippet: Changes in mRNA and protein levels of Kir4.1 and AQP4 in glyoxal-treated rMC-1 cells with or without ranibizumab. The ( a ) mRNA and ( b ) protein level of Kir4.1 in glyoxal-treated rMC-1 cells with or without ranibizumab. c Immunofluorescence of Kir4.1 in rMC-1 cells. The ( d ) mRNA and ( e ) protein level of AQP4 in glyoxal-treated rMC-1 cells with or without ranibizumab. f Immunofluorescence of AQP4 in rMC-1 cells. Data are expressed as mean ± SE (n = 8 in [a, d], n = 4 in [b, e], * P

    Techniques Used: Immunofluorescence

    Protein changes of ( a ) Kir4.1, ( b ) AQP4, ( c ) GS, and ( d ) GFAP in diabetic rat retinas treated with or without ranibizumab. e Co-immunostaining of Kir4.1 and GFAP in 12-week diabetic rat retinas (Kir4.1, green; GFAP, red; DAPI, blue), scale bar: 20 μm. Data are expressed as mean ± SE (n = 7, * P
    Figure Legend Snippet: Protein changes of ( a ) Kir4.1, ( b ) AQP4, ( c ) GS, and ( d ) GFAP in diabetic rat retinas treated with or without ranibizumab. e Co-immunostaining of Kir4.1 and GFAP in 12-week diabetic rat retinas (Kir4.1, green; GFAP, red; DAPI, blue), scale bar: 20 μm. Data are expressed as mean ± SE (n = 7, * P

    Techniques Used: Immunostaining

    4) Product Images from "Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer’s disease mouse model"

    Article Title: Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer’s disease mouse model

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-018-1264-8

    Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)
    Figure Legend Snippet: Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)

    Techniques Used: Immunofluorescence, Staining, Generated, Immunostaining

    Western blot analysis and quantification of astroglial marker proteins Aqp4, GS, Aldh1l1 and GFAP. Western blot analysis ( a ) was performed from four independently generated iAstro lines for each genotype (WT-iAstro#2, #3, #5 and #6, and 3Tg-iAstro#2, #3, #4 and #6). Each point represents mean ± SEM of 3 independent experiments. Actin was used as loading control. ANOVA followed by Tukey’s post-hoc test was used for statistical analysis. For Aqp4 ( b ) and GS ( c ) there were no significant differences. For Aldh1l1 ( d ) and GFAP ( e ) the differences were significant for iAstro lines vs primary astrocytes: ** p
    Figure Legend Snippet: Western blot analysis and quantification of astroglial marker proteins Aqp4, GS, Aldh1l1 and GFAP. Western blot analysis ( a ) was performed from four independently generated iAstro lines for each genotype (WT-iAstro#2, #3, #5 and #6, and 3Tg-iAstro#2, #3, #4 and #6). Each point represents mean ± SEM of 3 independent experiments. Actin was used as loading control. ANOVA followed by Tukey’s post-hoc test was used for statistical analysis. For Aqp4 ( b ) and GS ( c ) there were no significant differences. For Aldh1l1 ( d ) and GFAP ( e ) the differences were significant for iAstro lines vs primary astrocytes: ** p

    Techniques Used: Western Blot, Marker, Generated

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Alomone Labs aqp4
    Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with <t>anti-AQP4,</t> anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)
    Aqp4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aqp4/product/Alomone Labs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aqp4 - by Bioz Stars, 2022-09
    95/100 stars
      Buy from Supplier

    93
    Alomone Labs aqp2 4
    Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of <t>AQP2-4,</t> BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.
    Aqp2 4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aqp2 4/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aqp2 4 - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)

    Journal: Cell Death & Disease

    Article Title: Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer’s disease mouse model

    doi: 10.1038/s41419-018-1264-8

    Figure Lengend Snippet: Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)

    Article Snippet: The following primary antibodies were used: AQP4 (Alomone Labs, Cat. No. 249-323), Aldh1l1 (Abcam, Cat. No. Ab190298), GS (Abcam, Cat. No. Ab73593), GFAP (Chemicon International, Cat. No. CBL411) and GLT-1 (Alomone labs, Cat. No. AGC-022).

    Techniques: Immunofluorescence, Staining, Generated, Immunostaining

    Western blot analysis and quantification of astroglial marker proteins Aqp4, GS, Aldh1l1 and GFAP. Western blot analysis ( a ) was performed from four independently generated iAstro lines for each genotype (WT-iAstro#2, #3, #5 and #6, and 3Tg-iAstro#2, #3, #4 and #6). Each point represents mean ± SEM of 3 independent experiments. Actin was used as loading control. ANOVA followed by Tukey’s post-hoc test was used for statistical analysis. For Aqp4 ( b ) and GS ( c ) there were no significant differences. For Aldh1l1 ( d ) and GFAP ( e ) the differences were significant for iAstro lines vs primary astrocytes: ** p

    Journal: Cell Death & Disease

    Article Title: Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer’s disease mouse model

    doi: 10.1038/s41419-018-1264-8

    Figure Lengend Snippet: Western blot analysis and quantification of astroglial marker proteins Aqp4, GS, Aldh1l1 and GFAP. Western blot analysis ( a ) was performed from four independently generated iAstro lines for each genotype (WT-iAstro#2, #3, #5 and #6, and 3Tg-iAstro#2, #3, #4 and #6). Each point represents mean ± SEM of 3 independent experiments. Actin was used as loading control. ANOVA followed by Tukey’s post-hoc test was used for statistical analysis. For Aqp4 ( b ) and GS ( c ) there were no significant differences. For Aldh1l1 ( d ) and GFAP ( e ) the differences were significant for iAstro lines vs primary astrocytes: ** p

    Article Snippet: The following primary antibodies were used: AQP4 (Alomone Labs, Cat. No. 249-323), Aldh1l1 (Abcam, Cat. No. Ab190298), GS (Abcam, Cat. No. Ab73593), GFAP (Chemicon International, Cat. No. CBL411) and GLT-1 (Alomone labs, Cat. No. AGC-022).

    Techniques: Western Blot, Marker, Generated

    Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of AQP2-4, BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.

    Journal: Cells

    Article Title: Lithium Chloride and GSK3 Inhibition Reduce Aquaporin-2 Expression in Primary Cultured Inner Medullary Collecting Duct Cells Due to Independent Mechanisms

    doi: 10.3390/cells9041060

    Figure Lengend Snippet: Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of AQP2-4, BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.

    Article Snippet: Antibodies raised against Aqp2-4 were obtained from Alomone Labs (Jerusalem, Israel) and antibodies directed against phosphorylated Aqp2 at position S256 were a generous gift from M. Knepper (National Institutes of Health, Bethesda, MD, USA).

    Techniques: Concentration Assay, Expressing, Real-time Polymerase Chain Reaction

    MG132 and bafilomycin induce downregulation of Aqp2 on the mRNA level. IMCD cells were treated for 1 or 24 h with bafilomycin (Baf. 100 nM) or MG132 (2 µM) and the mRNA expression of Aqp2-4, BGT-1, and AR was compared to untreated cells by real-time PCR. One-way ANOVA with tukey post-test was performed and * indicates statistically significant differences compared untreated cells ( p ≤ 0.05).

    Journal: Cells

    Article Title: Lithium Chloride and GSK3 Inhibition Reduce Aquaporin-2 Expression in Primary Cultured Inner Medullary Collecting Duct Cells Due to Independent Mechanisms

    doi: 10.3390/cells9041060

    Figure Lengend Snippet: MG132 and bafilomycin induce downregulation of Aqp2 on the mRNA level. IMCD cells were treated for 1 or 24 h with bafilomycin (Baf. 100 nM) or MG132 (2 µM) and the mRNA expression of Aqp2-4, BGT-1, and AR was compared to untreated cells by real-time PCR. One-way ANOVA with tukey post-test was performed and * indicates statistically significant differences compared untreated cells ( p ≤ 0.05).

    Article Snippet: Antibodies raised against Aqp2-4 were obtained from Alomone Labs (Jerusalem, Israel) and antibodies directed against phosphorylated Aqp2 at position S256 were a generous gift from M. Knepper (National Institutes of Health, Bethesda, MD, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Migration influences the localization of AQPs. Wound healing assays were performed with IMCD-cells cultivated at 600 mosmol/kg. A scratch was induced and after 4 h the cells were stained for AQP2–4 using specific antibodies. For the AQP2 staining the cells were incubated with dbcAMP to induce membrane localization. Alexa-488 labeled secondary antibodies were used for visualization. The nuclei were stained with DAPI. The upper row show cells away from the wound. The lower rows show cells at the leading edge. Scale bar = 20 µm, representative image chosen from 6 to 9 independent experiments.

    Journal: Scientific Reports

    Article Title: Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells

    doi: 10.1038/s41598-021-91369-y

    Figure Lengend Snippet: Migration influences the localization of AQPs. Wound healing assays were performed with IMCD-cells cultivated at 600 mosmol/kg. A scratch was induced and after 4 h the cells were stained for AQP2–4 using specific antibodies. For the AQP2 staining the cells were incubated with dbcAMP to induce membrane localization. Alexa-488 labeled secondary antibodies were used for visualization. The nuclei were stained with DAPI. The upper row show cells away from the wound. The lower rows show cells at the leading edge. Scale bar = 20 µm, representative image chosen from 6 to 9 independent experiments.

    Article Snippet: Antibodies raised against AQP2–4 were obtained from Alomone Labs (Jerusalem, Israel) .

    Techniques: Migration, Staining, Incubation, Labeling