aqp4 antibodies  (Alomone Labs)


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    Structured Review

    Alomone Labs aqp4 antibodies
    Aqp4 Antibodies, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aqp4 antibodies/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aqp4 antibodies - by Bioz Stars, 2022-10
    93/100 stars

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    Alomone Labs aqp4
    Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with <t>anti-AQP4,</t> anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)
    Aqp4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aqp4/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aqp4 - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    93
    Alomone Labs aqp2 4
    Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of <t>AQP2-4,</t> BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.
    Aqp2 4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aqp2 4/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aqp2 4 - by Bioz Stars, 2022-10
    93/100 stars
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    94
    Alomone Labs anti aqp4
    The synthesized GILZ-p inhibited LPS induced Müller cell gliosis. Western blot analysis was performed to determine the protein expression levels of glial fibrillary acidic protein (GFAP) (A,B) , and <t>AQP4</t> (C,D) in Müller cells treated with 1000 ng/ml LPS in combination with different concentrations of GILZ-p (0.01, 0.1, 1, and 10 μM) for 24 h. β-actin was used as the loading control. The results of quantitative analysis, as determined by densitometric analysis, were expressed as relative to β-actin. Data represent the mean ± SE; the Mann–Whitney U -test was used for comparisons between two groups. n = 3 for each group. ∗ P
    Anti Aqp4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti aqp4/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti aqp4 - by Bioz Stars, 2022-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)

    Journal: Cell Death & Disease

    Article Title: Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer’s disease mouse model

    doi: 10.1038/s41419-018-1264-8

    Figure Lengend Snippet: Characterization of WT- and 3Tg-iAstro lines. a Phase contrast images of four WT-iAstro and four 3Tg-iAstro lines at passage 15. Bar, 100 μm. b Immunofluorescence images of WT-iAstro#2 and 3Tg-iAstro#2, stained with anti-AQP4, anti-GS, anti-Aldh1l1 and anti-GFAP antibodies. Bar, 50 μm. The images shown in ( a , b ) are representative of n = 3 independent experiments. c Quantification of GFAP-positive cells in WT-iAstro#2 and 3Tg-iAstro#2 lines. Data expressed as mean ± SD % of 15 fields of GFAP-positive cells evaluating a total of 359 WT- and 514 3Tg-iAstro cells. In ( b , c ), other characterized independently generated iAstro lines show similar results in immunostaining of astroglial markers and in quantification of GFAP-positive cells (data not shown)

    Article Snippet: The following primary antibodies were used: AQP4 (Alomone Labs, Cat. No. 249-323), Aldh1l1 (Abcam, Cat. No. Ab190298), GS (Abcam, Cat. No. Ab73593), GFAP (Chemicon International, Cat. No. CBL411) and GLT-1 (Alomone labs, Cat. No. AGC-022).

    Techniques: Immunofluorescence, Staining, Generated, Immunostaining

    Western blot analysis and quantification of astroglial marker proteins Aqp4, GS, Aldh1l1 and GFAP. Western blot analysis ( a ) was performed from four independently generated iAstro lines for each genotype (WT-iAstro#2, #3, #5 and #6, and 3Tg-iAstro#2, #3, #4 and #6). Each point represents mean ± SEM of 3 independent experiments. Actin was used as loading control. ANOVA followed by Tukey’s post-hoc test was used for statistical analysis. For Aqp4 ( b ) and GS ( c ) there were no significant differences. For Aldh1l1 ( d ) and GFAP ( e ) the differences were significant for iAstro lines vs primary astrocytes: ** p

    Journal: Cell Death & Disease

    Article Title: Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer’s disease mouse model

    doi: 10.1038/s41419-018-1264-8

    Figure Lengend Snippet: Western blot analysis and quantification of astroglial marker proteins Aqp4, GS, Aldh1l1 and GFAP. Western blot analysis ( a ) was performed from four independently generated iAstro lines for each genotype (WT-iAstro#2, #3, #5 and #6, and 3Tg-iAstro#2, #3, #4 and #6). Each point represents mean ± SEM of 3 independent experiments. Actin was used as loading control. ANOVA followed by Tukey’s post-hoc test was used for statistical analysis. For Aqp4 ( b ) and GS ( c ) there were no significant differences. For Aldh1l1 ( d ) and GFAP ( e ) the differences were significant for iAstro lines vs primary astrocytes: ** p

    Article Snippet: The following primary antibodies were used: AQP4 (Alomone Labs, Cat. No. 249-323), Aldh1l1 (Abcam, Cat. No. Ab190298), GS (Abcam, Cat. No. Ab73593), GFAP (Chemicon International, Cat. No. CBL411) and GLT-1 (Alomone labs, Cat. No. AGC-022).

    Techniques: Western Blot, Marker, Generated

    Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of AQP2-4, BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.

    Journal: Cells

    Article Title: Lithium Chloride and GSK3 Inhibition Reduce Aquaporin-2 Expression in Primary Cultured Inner Medullary Collecting Duct Cells Due to Independent Mechanisms

    doi: 10.3390/cells9041060

    Figure Lengend Snippet: Downregulation of AQP2 mRNA by LiCl is time and concentration dependent. IMCD cells were treated for different time points with LiCl (20 mM). The mRNA expression of AQP2-4, BGT1, and AR was analyzed by real-time PCR and the relative changes compared to untreated cells were calculated ( a ). In the same way, IMCD cells were treated for 24 h with 10 or 20 mM of LiCl and the relative changes in the gene expression of AQP2 and AR were compared to untreated cells ( b ). One-way ANOVA analysis with Tukey post-test, * indicates statistically significant differences to untreated cells ( p ≤ 0.05); n = 3.

    Article Snippet: Antibodies raised against Aqp2-4 were obtained from Alomone Labs (Jerusalem, Israel) and antibodies directed against phosphorylated Aqp2 at position S256 were a generous gift from M. Knepper (National Institutes of Health, Bethesda, MD, USA).

    Techniques: Concentration Assay, Expressing, Real-time Polymerase Chain Reaction

    MG132 and bafilomycin induce downregulation of Aqp2 on the mRNA level. IMCD cells were treated for 1 or 24 h with bafilomycin (Baf. 100 nM) or MG132 (2 µM) and the mRNA expression of Aqp2-4, BGT-1, and AR was compared to untreated cells by real-time PCR. One-way ANOVA with tukey post-test was performed and * indicates statistically significant differences compared untreated cells ( p ≤ 0.05).

    Journal: Cells

    Article Title: Lithium Chloride and GSK3 Inhibition Reduce Aquaporin-2 Expression in Primary Cultured Inner Medullary Collecting Duct Cells Due to Independent Mechanisms

    doi: 10.3390/cells9041060

    Figure Lengend Snippet: MG132 and bafilomycin induce downregulation of Aqp2 on the mRNA level. IMCD cells were treated for 1 or 24 h with bafilomycin (Baf. 100 nM) or MG132 (2 µM) and the mRNA expression of Aqp2-4, BGT-1, and AR was compared to untreated cells by real-time PCR. One-way ANOVA with tukey post-test was performed and * indicates statistically significant differences compared untreated cells ( p ≤ 0.05).

    Article Snippet: Antibodies raised against Aqp2-4 were obtained from Alomone Labs (Jerusalem, Israel) and antibodies directed against phosphorylated Aqp2 at position S256 were a generous gift from M. Knepper (National Institutes of Health, Bethesda, MD, USA).

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Migration influences the localization of AQPs. Wound healing assays were performed with IMCD-cells cultivated at 600 mosmol/kg. A scratch was induced and after 4 h the cells were stained for AQP2–4 using specific antibodies. For the AQP2 staining the cells were incubated with dbcAMP to induce membrane localization. Alexa-488 labeled secondary antibodies were used for visualization. The nuclei were stained with DAPI. The upper row show cells away from the wound. The lower rows show cells at the leading edge. Scale bar = 20 µm, representative image chosen from 6 to 9 independent experiments.

    Journal: Scientific Reports

    Article Title: Unexpected localization of AQP3 and AQP4 induced by migration of primary cultured IMCD cells

    doi: 10.1038/s41598-021-91369-y

    Figure Lengend Snippet: Migration influences the localization of AQPs. Wound healing assays were performed with IMCD-cells cultivated at 600 mosmol/kg. A scratch was induced and after 4 h the cells were stained for AQP2–4 using specific antibodies. For the AQP2 staining the cells were incubated with dbcAMP to induce membrane localization. Alexa-488 labeled secondary antibodies were used for visualization. The nuclei were stained with DAPI. The upper row show cells away from the wound. The lower rows show cells at the leading edge. Scale bar = 20 µm, representative image chosen from 6 to 9 independent experiments.

    Article Snippet: Antibodies raised against AQP2–4 were obtained from Alomone Labs (Jerusalem, Israel) .

    Techniques: Migration, Staining, Incubation, Labeling

    The synthesized GILZ-p inhibited LPS induced Müller cell gliosis. Western blot analysis was performed to determine the protein expression levels of glial fibrillary acidic protein (GFAP) (A,B) , and AQP4 (C,D) in Müller cells treated with 1000 ng/ml LPS in combination with different concentrations of GILZ-p (0.01, 0.1, 1, and 10 μM) for 24 h. β-actin was used as the loading control. The results of quantitative analysis, as determined by densitometric analysis, were expressed as relative to β-actin. Data represent the mean ± SE; the Mann–Whitney U -test was used for comparisons between two groups. n = 3 for each group. ∗ P

    Journal: Frontiers in Pharmacology

    Article Title: A Synthesized Glucocorticoid- Induced Leucine Zipper Peptide Inhibits Retinal Müller Cell Gliosis

    doi: 10.3389/fphar.2018.00331

    Figure Lengend Snippet: The synthesized GILZ-p inhibited LPS induced Müller cell gliosis. Western blot analysis was performed to determine the protein expression levels of glial fibrillary acidic protein (GFAP) (A,B) , and AQP4 (C,D) in Müller cells treated with 1000 ng/ml LPS in combination with different concentrations of GILZ-p (0.01, 0.1, 1, and 10 μM) for 24 h. β-actin was used as the loading control. The results of quantitative analysis, as determined by densitometric analysis, were expressed as relative to β-actin. Data represent the mean ± SE; the Mann–Whitney U -test was used for comparisons between two groups. n = 3 for each group. ∗ P

    Article Snippet: The membranes were blocked in 5% non-fat milk at room temperature for 1 h and incubated with the following antibodies: anti-monocyte chemoattractant protein (MCP)-1 (ab25124; Abcam, Cambridge, United Kingdom), anti-intercellular adhesion molecule (ICAM)-1 (ab171123; Proteintech, Chicago, IL, United States), anti-IL1β (ab9787; Abcam), Anti-tumor necrosis factor (TNF)α (PB0270, Boster Biological Technology, Wuhan, China), rabbit anti-p65 polyclonal antibody (ab16502; Abcam), anti-phospho-NF-κB p65 (Ser536) rabbit monoclonal antibody (3033P; Cell Signaling Technology, Beverly, MA, United States), anti-AQP4 (300-314, Alomone Labs, Jerusalem, Israel), anti-glial fibrillary acidic protein (GFAP) (ab10062, Abcam), or rabbit anti β-actin antibody (ab69512; Abcam) overnight.

    Techniques: Synthesized, Western Blot, Expressing, MANN-WHITNEY