p2y6  (Alomone Labs)


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    Alomone Labs p2y6
    P2y6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    p2y6  (Alomone Labs)


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    Alomone Labs p2y6
    P2y6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p2y6  (Alomone Labs)


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    Alomone Labs p2y6
    P2y6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y6/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
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    anti p2y6 receptor fitc  (Alomone Labs)


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    Alomone Labs anti p2y6 receptor fitc
    Decrease of <t>P2Y6</t> Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a <t>FITC-conjugated</t> antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p<0.05 (*<0.05, **<0.005,***<0.0005, ****<0.0001).
    Anti P2y6 Receptor Fitc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    anti p2y6 receptor fitc - by Bioz Stars, 2023-02
    93/100 stars

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    1) Product Images from "The Inhibitory Effect of Curosurf ® and Alveofact ® on the Formation of Neutrophil Extracellular Traps"

    Article Title: The Inhibitory Effect of Curosurf ® and Alveofact ® on the Formation of Neutrophil Extracellular Traps

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2020.582895

    Decrease of P2Y6 Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a FITC-conjugated antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p<0.05 (*<0.05, **<0.005,***<0.0005, ****<0.0001).
    Figure Legend Snippet: Decrease of P2Y6 Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a FITC-conjugated antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p<0.05 (*<0.05, **<0.005,***<0.0005, ****<0.0001).

    Techniques Used: Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay

    p2y 6  (Alomone Labs)


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    Alomone Labs p2y 6
    Expression profiling of the <t>P2Y</t> receptor family in the cortex following status epilepticus. (A) Photomicrograph (20× lens) showing neuronal damage 24 h following intraamygdala KA-induced status epilepticus in the ipsilateral hippocampus and cortex. Scale bar = 100 μm. (B) Representative Western blot (n = 1 per lane) and corresponding graph showing increased c-Fos expression in the ipsilateral cortex post-status epilepticus (n = 4 per group). (C) Representative Western blots (n = 1 per lane) and corresponding graphs showing the expression of the different P2Y receptor family members P2Y 1 , P2Y 2 , P2Y 4 , <t>P2Y</t> <t>6</t> , P2Y 12 , P2Y 13 , and P2Y 14 in cortical tissue following status epilepticus. Of note, while P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 are significantly increased post-status epilepticus, no changes could be observed for the remaining P2Y receptors (n = 6 per group). * p < 0.05, ** p < 0.01.
    P2y 6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y 1 Antagonism in Mice"

    Article Title: Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y 1 Antagonism in Mice

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2019.01558

    Expression profiling of the P2Y receptor family in the cortex following status epilepticus. (A) Photomicrograph (20× lens) showing neuronal damage 24 h following intraamygdala KA-induced status epilepticus in the ipsilateral hippocampus and cortex. Scale bar = 100 μm. (B) Representative Western blot (n = 1 per lane) and corresponding graph showing increased c-Fos expression in the ipsilateral cortex post-status epilepticus (n = 4 per group). (C) Representative Western blots (n = 1 per lane) and corresponding graphs showing the expression of the different P2Y receptor family members P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 , P2Y 13 , and P2Y 14 in cortical tissue following status epilepticus. Of note, while P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 are significantly increased post-status epilepticus, no changes could be observed for the remaining P2Y receptors (n = 6 per group). * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: Expression profiling of the P2Y receptor family in the cortex following status epilepticus. (A) Photomicrograph (20× lens) showing neuronal damage 24 h following intraamygdala KA-induced status epilepticus in the ipsilateral hippocampus and cortex. Scale bar = 100 μm. (B) Representative Western blot (n = 1 per lane) and corresponding graph showing increased c-Fos expression in the ipsilateral cortex post-status epilepticus (n = 4 per group). (C) Representative Western blots (n = 1 per lane) and corresponding graphs showing the expression of the different P2Y receptor family members P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 , P2Y 13 , and P2Y 14 in cortical tissue following status epilepticus. Of note, while P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 are significantly increased post-status epilepticus, no changes could be observed for the remaining P2Y receptors (n = 6 per group). * p < 0.05, ** p < 0.01.

    Techniques Used: Expressing, Western Blot

    Cell-specific expression of P2Y 1 post-status epilepticus. (A) Photomicrographs (40× lens) showing co-localization of P2Y 1 (green) with neuronal marker NeuN in cortical tissue (layer V–VI) in control injected-vehicle mice and following status epilepticus (SE) (white arrows). Scale bar = 10 µm (B) Co-localization of P2Y 1 (green) with microglia marker Iba1 (red) 24 h following status epilepticus (SE) in the cortex (white arrows). Scale bar = 10 μm. (C, D) No co-localization of P2Y 1 (green) with the astrocyte markers GFAP and S100β (red). Scale bar = 10 μm. (E) Graph showing strong increase in P2Y 1 -positive microglia 24 h following status epilepticus (n = 3 per group). (F) Enlarged images, outlined by white box, showing co-localization of P2Y 1 (green) on neurons (red) and microglia (red) 24 h post-status epilepticus (SE). Scale bar = 10 μm. (G) Specificity of P2Y 1 -detecting antibody was confirmed using tissue from P2Y 1 knockout mice (KO) 8 h post-status epilepticus. Scale bar = 10 μm. Images are representative image from three animals per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: Cell-specific expression of P2Y 1 post-status epilepticus. (A) Photomicrographs (40× lens) showing co-localization of P2Y 1 (green) with neuronal marker NeuN in cortical tissue (layer V–VI) in control injected-vehicle mice and following status epilepticus (SE) (white arrows). Scale bar = 10 µm (B) Co-localization of P2Y 1 (green) with microglia marker Iba1 (red) 24 h following status epilepticus (SE) in the cortex (white arrows). Scale bar = 10 μm. (C, D) No co-localization of P2Y 1 (green) with the astrocyte markers GFAP and S100β (red). Scale bar = 10 μm. (E) Graph showing strong increase in P2Y 1 -positive microglia 24 h following status epilepticus (n = 3 per group). (F) Enlarged images, outlined by white box, showing co-localization of P2Y 1 (green) on neurons (red) and microglia (red) 24 h post-status epilepticus (SE). Scale bar = 10 μm. (G) Specificity of P2Y 1 -detecting antibody was confirmed using tissue from P2Y 1 knockout mice (KO) 8 h post-status epilepticus. Scale bar = 10 μm. Images are representative image from three animals per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Expressing, Marker, Injection, Knock-Out

    P2Y 1 antagonism decreases high frequency high amplitude spiking during status epilepticus. (A) Representative EEG traces recorded from the cortex from the time-point of intraamygdala kainic acid (KA) injection until 60 min post-lorazepam (Lz) of mice treated with vehicle (Veh), P2Y 1 agonist MRS2365 (MRS23), and P2Y 1 antagonist MRS2500 (MRS25). Treatment with P2Y 1 -targeting drugs was administered 15 min post-intraamygdala KA injection via i.c.v. Lorazepam (Lz) was administered 40 min following KA injection via i.p. (B) Representative EEG traces showing examples of high frequency and high amplitude (HFHA) spiking taken during the 30 min recording period following drug treatment (see arrows). Mice treated with the P2Y 1 agonist MRS23 showed an increase in the duration of HFHA spiking while mice treated with the P2Y 1 antagonists MRS25 showed a decrease in the duration of HFHA spiking during status epilepticus (n = 7 Veh, 9 MRS23, and 8 MRS25). (C) Representative EEG traces and graph showing slightly increased duration of HFHA spiking during status epilepticus in mice treated with minocycline and P2Y 1 antagonists MRS25 (n = 4 per group). * p < 0.05, ** p < 0.01.
    Figure Legend Snippet: P2Y 1 antagonism decreases high frequency high amplitude spiking during status epilepticus. (A) Representative EEG traces recorded from the cortex from the time-point of intraamygdala kainic acid (KA) injection until 60 min post-lorazepam (Lz) of mice treated with vehicle (Veh), P2Y 1 agonist MRS2365 (MRS23), and P2Y 1 antagonist MRS2500 (MRS25). Treatment with P2Y 1 -targeting drugs was administered 15 min post-intraamygdala KA injection via i.c.v. Lorazepam (Lz) was administered 40 min following KA injection via i.p. (B) Representative EEG traces showing examples of high frequency and high amplitude (HFHA) spiking taken during the 30 min recording period following drug treatment (see arrows). Mice treated with the P2Y 1 agonist MRS23 showed an increase in the duration of HFHA spiking while mice treated with the P2Y 1 antagonists MRS25 showed a decrease in the duration of HFHA spiking during status epilepticus (n = 7 Veh, 9 MRS23, and 8 MRS25). (C) Representative EEG traces and graph showing slightly increased duration of HFHA spiking during status epilepticus in mice treated with minocycline and P2Y 1 antagonists MRS25 (n = 4 per group). * p < 0.05, ** p < 0.01.

    Techniques Used: Injection

    Decreased status epilepticus-induced cortical damage via P2Y 1 antagonism. (A) Representative images (20× lens) and corresponding graph showing a decrease in neuronal damage in the cortex 24 h post-status epilepticus in mice treated with the P2Y 1 antagonist MRS25 (n = 7 Veh, 9 MRS23, and 8 MRS25). (B) Representative images (20x lens) and corresponding graph showing slightly more Fluoro-Jade B (FjB)-positive cells in ipsilateral cortex 24 h post-status epilepticus in mice treated with both minocycline and the P2Y1 antagonist MRS25 (n = 4/group). ** p < 0.01.
    Figure Legend Snippet: Decreased status epilepticus-induced cortical damage via P2Y 1 antagonism. (A) Representative images (20× lens) and corresponding graph showing a decrease in neuronal damage in the cortex 24 h post-status epilepticus in mice treated with the P2Y 1 antagonist MRS25 (n = 7 Veh, 9 MRS23, and 8 MRS25). (B) Representative images (20x lens) and corresponding graph showing slightly more Fluoro-Jade B (FjB)-positive cells in ipsilateral cortex 24 h post-status epilepticus in mice treated with both minocycline and the P2Y1 antagonist MRS25 (n = 4/group). ** p < 0.01.

    Techniques Used:

    p2y 6  (Alomone Labs)


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    Alomone Labs p2y 6
    Expression of ADP‐activated <t>P2Y</t> receptors in THP‐1 cells. (A) RT‐PCR analysis of P2Y1 (326 bp), P2Y6 (391 bp), P2Y12 (698 bp) and P2Y13 (461 bp) receptor expression in THP‐1 monocytes. RT‐PCR analysis of P2Y12 receptors (698) expression in freshly isolated CD14+ monocytes from human peripheral blood. For (A) and (B): predicted PCR ampilicon size given in parentheses; no RT (no reverse transcriptase) denotes negative control experiments for genomic DNA contamination. (C) Representative confocal microscopy images showing P2Y primary antibody immunoreactivity in fixed THP‐1 cells. Cells are labelled with polyclonal antibodies against P2Y receptor subunits and fluorescence (green) visualized by using a AF488‐conjugated secondary antibody (lefthand panels). The “control” is representative of an experiment where primary antibodies have been omitted. Cells are counterstained with diamidino‐2‐phenylindole to identify nuclei (blue; in overlay in righthand panel). Scale bar is 5 μm. Experiments are representative of at least three independent experiments.
    P2y 6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    1) Product Images from "P2Y 12 receptor modulation of ADP ‐evoked intracellular Ca 2+ signalling in THP‐1 human monocytic cells"

    Article Title: P2Y 12 receptor modulation of ADP ‐evoked intracellular Ca 2+ signalling in THP‐1 human monocytic cells

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.14218

    Expression of ADP‐activated P2Y receptors in THP‐1 cells. (A) RT‐PCR analysis of P2Y1 (326 bp), P2Y6 (391 bp), P2Y12 (698 bp) and P2Y13 (461 bp) receptor expression in THP‐1 monocytes. RT‐PCR analysis of P2Y12 receptors (698) expression in freshly isolated CD14+ monocytes from human peripheral blood. For (A) and (B): predicted PCR ampilicon size given in parentheses; no RT (no reverse transcriptase) denotes negative control experiments for genomic DNA contamination. (C) Representative confocal microscopy images showing P2Y primary antibody immunoreactivity in fixed THP‐1 cells. Cells are labelled with polyclonal antibodies against P2Y receptor subunits and fluorescence (green) visualized by using a AF488‐conjugated secondary antibody (lefthand panels). The “control” is representative of an experiment where primary antibodies have been omitted. Cells are counterstained with diamidino‐2‐phenylindole to identify nuclei (blue; in overlay in righthand panel). Scale bar is 5 μm. Experiments are representative of at least three independent experiments.
    Figure Legend Snippet: Expression of ADP‐activated P2Y receptors in THP‐1 cells. (A) RT‐PCR analysis of P2Y1 (326 bp), P2Y6 (391 bp), P2Y12 (698 bp) and P2Y13 (461 bp) receptor expression in THP‐1 monocytes. RT‐PCR analysis of P2Y12 receptors (698) expression in freshly isolated CD14+ monocytes from human peripheral blood. For (A) and (B): predicted PCR ampilicon size given in parentheses; no RT (no reverse transcriptase) denotes negative control experiments for genomic DNA contamination. (C) Representative confocal microscopy images showing P2Y primary antibody immunoreactivity in fixed THP‐1 cells. Cells are labelled with polyclonal antibodies against P2Y receptor subunits and fluorescence (green) visualized by using a AF488‐conjugated secondary antibody (lefthand panels). The “control” is representative of an experiment where primary antibodies have been omitted. Cells are counterstained with diamidino‐2‐phenylindole to identify nuclei (blue; in overlay in righthand panel). Scale bar is 5 μm. Experiments are representative of at least three independent experiments.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Negative Control, Confocal Microscopy, Fluorescence

    antibody 106 cells  (Alomone Labs)


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    Alomone Labs antibody 106 cells
    Antibody 106 Cells, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p2y 6  (Alomone Labs)


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    Alomone Labs p2y 6
    Primary and secondary antibodies used in immunohistochemistry experiments
    P2y 6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Activation of P2Y 6 receptors increases the voiding frequency in anaesthetized rats by releasing ATP from the bladder urothelium"

    Article Title: Activation of P2Y 6 receptors increases the voiding frequency in anaesthetized rats by releasing ATP from the bladder urothelium

    Journal: British Journal of Pharmacology

    doi: 10.1111/bph.12711

    Primary and secondary antibodies used in immunohistochemistry experiments
    Figure Legend Snippet: Primary and secondary antibodies used in immunohistochemistry experiments

    Techniques Used: Immunohistochemistry

    p2y 6  (Alomone Labs)


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    Alomone Labs p2y 6
    P2y 6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    p2y6 antibody unavailable  (Alomone Labs)


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    Alomone Labs p2y6 antibody unavailable
    P2y6 Antibody Unavailable, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs p2y6
    P2y6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs anti p2y6 receptor fitc
    Decrease of <t>P2Y6</t> Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a <t>FITC-conjugated</t> antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p<0.05 (*<0.05, **<0.005,***<0.0005, ****<0.0001).
    Anti P2y6 Receptor Fitc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Alomone Labs p2y 6
    Expression profiling of the <t>P2Y</t> receptor family in the cortex following status epilepticus. (A) Photomicrograph (20× lens) showing neuronal damage 24 h following intraamygdala KA-induced status epilepticus in the ipsilateral hippocampus and cortex. Scale bar = 100 μm. (B) Representative Western blot (n = 1 per lane) and corresponding graph showing increased c-Fos expression in the ipsilateral cortex post-status epilepticus (n = 4 per group). (C) Representative Western blots (n = 1 per lane) and corresponding graphs showing the expression of the different P2Y receptor family members P2Y 1 , P2Y 2 , P2Y 4 , <t>P2Y</t> <t>6</t> , P2Y 12 , P2Y 13 , and P2Y 14 in cortical tissue following status epilepticus. Of note, while P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 are significantly increased post-status epilepticus, no changes could be observed for the remaining P2Y receptors (n = 6 per group). * p < 0.05, ** p < 0.01.
    P2y 6, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Alomone Labs antibody 106 cells
    Expression profiling of the <t>P2Y</t> receptor family in the cortex following status epilepticus. (A) Photomicrograph (20× lens) showing neuronal damage 24 h following intraamygdala KA-induced status epilepticus in the ipsilateral hippocampus and cortex. Scale bar = 100 μm. (B) Representative Western blot (n = 1 per lane) and corresponding graph showing increased c-Fos expression in the ipsilateral cortex post-status epilepticus (n = 4 per group). (C) Representative Western blots (n = 1 per lane) and corresponding graphs showing the expression of the different P2Y receptor family members P2Y 1 , P2Y 2 , P2Y 4 , <t>P2Y</t> <t>6</t> , P2Y 12 , P2Y 13 , and P2Y 14 in cortical tissue following status epilepticus. Of note, while P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 are significantly increased post-status epilepticus, no changes could be observed for the remaining P2Y receptors (n = 6 per group). * p < 0.05, ** p < 0.01.
    Antibody 106 Cells, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Alomone Labs p2y6 antibody unavailable
    Expression profiling of the <t>P2Y</t> receptor family in the cortex following status epilepticus. (A) Photomicrograph (20× lens) showing neuronal damage 24 h following intraamygdala KA-induced status epilepticus in the ipsilateral hippocampus and cortex. Scale bar = 100 μm. (B) Representative Western blot (n = 1 per lane) and corresponding graph showing increased c-Fos expression in the ipsilateral cortex post-status epilepticus (n = 4 per group). (C) Representative Western blots (n = 1 per lane) and corresponding graphs showing the expression of the different P2Y receptor family members P2Y 1 , P2Y 2 , P2Y 4 , <t>P2Y</t> <t>6</t> , P2Y 12 , P2Y 13 , and P2Y 14 in cortical tissue following status epilepticus. Of note, while P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 are significantly increased post-status epilepticus, no changes could be observed for the remaining P2Y receptors (n = 6 per group). * p < 0.05, ** p < 0.01.
    P2y6 Antibody Unavailable, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p2y6 antibody unavailable/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
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    p2y6 antibody unavailable - by Bioz Stars, 2023-02
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    Image Search Results


    Decrease of P2Y6 Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a FITC-conjugated antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p<0.05 (*<0.05, **<0.005,***<0.0005, ****<0.0001).

    Journal: Frontiers in Immunology

    Article Title: The Inhibitory Effect of Curosurf ® and Alveofact ® on the Formation of Neutrophil Extracellular Traps

    doi: 10.3389/fimmu.2020.582895

    Figure Lengend Snippet: Decrease of P2Y6 Receptor and Calcium mobilization after treatment without change in intracellular PAD4 levels. (A) Neutrophils were treated as described before. P2Y6 was measured by flow cytometry with a FITC-conjugated antibody. Treatment with Alveofact ® decreases extracellular expression of the P2Y6 receptor independent of timing or dosing, while Curosurf ® does not exhibit this effect in every dosing. (B) Calcium levels at 3 h after treatment were used. Data were normalized to stimulated PMA control due to the heterogeneity in basic calcium levels and the response of the neutrophils to stimulation. Results are similar to the findings of the P2Y6 receptor expression. (C) Intracellular PAD4 levels were determined by ELISA using cell lysates of 1×10 6 cells. Interestingly, PAD4 levels did not change after treatment with surfactant compared to PMA-stimulated neutrophils. Significance level was set as p<0.05 (*<0.05, **<0.005,***<0.0005, ****<0.0001).

    Article Snippet: Cells were washed twice with PBS (Thermo Fischer, Waltham, MA, USA) and labeled with propidium iodide (PI) and Annexin-V-FITC (Becton, Dickinson and Company, Franklin Lakes, NJ, USA) or anti-CD11b-VioBlue (mAb REA713, IgG1) and anti-CD66b-APC (mAb REA306, IgG1) antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) or anti-P2Y6 Receptor-FITC (Alomone Labs, Jerusalem, Israel) as described in the manufacturer’s protocols.

    Techniques: Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay

    Expression profiling of the P2Y receptor family in the cortex following status epilepticus. (A) Photomicrograph (20× lens) showing neuronal damage 24 h following intraamygdala KA-induced status epilepticus in the ipsilateral hippocampus and cortex. Scale bar = 100 μm. (B) Representative Western blot (n = 1 per lane) and corresponding graph showing increased c-Fos expression in the ipsilateral cortex post-status epilepticus (n = 4 per group). (C) Representative Western blots (n = 1 per lane) and corresponding graphs showing the expression of the different P2Y receptor family members P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 , P2Y 13 , and P2Y 14 in cortical tissue following status epilepticus. Of note, while P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 are significantly increased post-status epilepticus, no changes could be observed for the remaining P2Y receptors (n = 6 per group). * p < 0.05, ** p < 0.01.

    Journal: Frontiers in Pharmacology

    Article Title: Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y 1 Antagonism in Mice

    doi: 10.3389/fphar.2019.01558

    Figure Lengend Snippet: Expression profiling of the P2Y receptor family in the cortex following status epilepticus. (A) Photomicrograph (20× lens) showing neuronal damage 24 h following intraamygdala KA-induced status epilepticus in the ipsilateral hippocampus and cortex. Scale bar = 100 μm. (B) Representative Western blot (n = 1 per lane) and corresponding graph showing increased c-Fos expression in the ipsilateral cortex post-status epilepticus (n = 4 per group). (C) Representative Western blots (n = 1 per lane) and corresponding graphs showing the expression of the different P2Y receptor family members P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 , P2Y 13 , and P2Y 14 in cortical tissue following status epilepticus. Of note, while P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 are significantly increased post-status epilepticus, no changes could be observed for the remaining P2Y receptors (n = 6 per group). * p < 0.05, ** p < 0.01.

    Article Snippet: Membranes were probed with antibodies against P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 , P2Y 13 , and P2Y 14 (Alomone Labs, Hadassah Ein Kerem, Jerusalem, Israel), c-Fos (Santa Cruz, Heidelberg, Germany), and β-actin (Sigma-Aldrich, Dublin, Ireland).

    Techniques: Expressing, Western Blot

    Cell-specific expression of P2Y 1 post-status epilepticus. (A) Photomicrographs (40× lens) showing co-localization of P2Y 1 (green) with neuronal marker NeuN in cortical tissue (layer V–VI) in control injected-vehicle mice and following status epilepticus (SE) (white arrows). Scale bar = 10 µm (B) Co-localization of P2Y 1 (green) with microglia marker Iba1 (red) 24 h following status epilepticus (SE) in the cortex (white arrows). Scale bar = 10 μm. (C, D) No co-localization of P2Y 1 (green) with the astrocyte markers GFAP and S100β (red). Scale bar = 10 μm. (E) Graph showing strong increase in P2Y 1 -positive microglia 24 h following status epilepticus (n = 3 per group). (F) Enlarged images, outlined by white box, showing co-localization of P2Y 1 (green) on neurons (red) and microglia (red) 24 h post-status epilepticus (SE). Scale bar = 10 μm. (G) Specificity of P2Y 1 -detecting antibody was confirmed using tissue from P2Y 1 knockout mice (KO) 8 h post-status epilepticus. Scale bar = 10 μm. Images are representative image from three animals per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Frontiers in Pharmacology

    Article Title: Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y 1 Antagonism in Mice

    doi: 10.3389/fphar.2019.01558

    Figure Lengend Snippet: Cell-specific expression of P2Y 1 post-status epilepticus. (A) Photomicrographs (40× lens) showing co-localization of P2Y 1 (green) with neuronal marker NeuN in cortical tissue (layer V–VI) in control injected-vehicle mice and following status epilepticus (SE) (white arrows). Scale bar = 10 µm (B) Co-localization of P2Y 1 (green) with microglia marker Iba1 (red) 24 h following status epilepticus (SE) in the cortex (white arrows). Scale bar = 10 μm. (C, D) No co-localization of P2Y 1 (green) with the astrocyte markers GFAP and S100β (red). Scale bar = 10 μm. (E) Graph showing strong increase in P2Y 1 -positive microglia 24 h following status epilepticus (n = 3 per group). (F) Enlarged images, outlined by white box, showing co-localization of P2Y 1 (green) on neurons (red) and microglia (red) 24 h post-status epilepticus (SE). Scale bar = 10 μm. (G) Specificity of P2Y 1 -detecting antibody was confirmed using tissue from P2Y 1 knockout mice (KO) 8 h post-status epilepticus. Scale bar = 10 μm. Images are representative image from three animals per experiment. * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: Membranes were probed with antibodies against P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 , P2Y 13 , and P2Y 14 (Alomone Labs, Hadassah Ein Kerem, Jerusalem, Israel), c-Fos (Santa Cruz, Heidelberg, Germany), and β-actin (Sigma-Aldrich, Dublin, Ireland).

    Techniques: Expressing, Marker, Injection, Knock-Out

    P2Y 1 antagonism decreases high frequency high amplitude spiking during status epilepticus. (A) Representative EEG traces recorded from the cortex from the time-point of intraamygdala kainic acid (KA) injection until 60 min post-lorazepam (Lz) of mice treated with vehicle (Veh), P2Y 1 agonist MRS2365 (MRS23), and P2Y 1 antagonist MRS2500 (MRS25). Treatment with P2Y 1 -targeting drugs was administered 15 min post-intraamygdala KA injection via i.c.v. Lorazepam (Lz) was administered 40 min following KA injection via i.p. (B) Representative EEG traces showing examples of high frequency and high amplitude (HFHA) spiking taken during the 30 min recording period following drug treatment (see arrows). Mice treated with the P2Y 1 agonist MRS23 showed an increase in the duration of HFHA spiking while mice treated with the P2Y 1 antagonists MRS25 showed a decrease in the duration of HFHA spiking during status epilepticus (n = 7 Veh, 9 MRS23, and 8 MRS25). (C) Representative EEG traces and graph showing slightly increased duration of HFHA spiking during status epilepticus in mice treated with minocycline and P2Y 1 antagonists MRS25 (n = 4 per group). * p < 0.05, ** p < 0.01.

    Journal: Frontiers in Pharmacology

    Article Title: Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y 1 Antagonism in Mice

    doi: 10.3389/fphar.2019.01558

    Figure Lengend Snippet: P2Y 1 antagonism decreases high frequency high amplitude spiking during status epilepticus. (A) Representative EEG traces recorded from the cortex from the time-point of intraamygdala kainic acid (KA) injection until 60 min post-lorazepam (Lz) of mice treated with vehicle (Veh), P2Y 1 agonist MRS2365 (MRS23), and P2Y 1 antagonist MRS2500 (MRS25). Treatment with P2Y 1 -targeting drugs was administered 15 min post-intraamygdala KA injection via i.c.v. Lorazepam (Lz) was administered 40 min following KA injection via i.p. (B) Representative EEG traces showing examples of high frequency and high amplitude (HFHA) spiking taken during the 30 min recording period following drug treatment (see arrows). Mice treated with the P2Y 1 agonist MRS23 showed an increase in the duration of HFHA spiking while mice treated with the P2Y 1 antagonists MRS25 showed a decrease in the duration of HFHA spiking during status epilepticus (n = 7 Veh, 9 MRS23, and 8 MRS25). (C) Representative EEG traces and graph showing slightly increased duration of HFHA spiking during status epilepticus in mice treated with minocycline and P2Y 1 antagonists MRS25 (n = 4 per group). * p < 0.05, ** p < 0.01.

    Article Snippet: Membranes were probed with antibodies against P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 , P2Y 13 , and P2Y 14 (Alomone Labs, Hadassah Ein Kerem, Jerusalem, Israel), c-Fos (Santa Cruz, Heidelberg, Germany), and β-actin (Sigma-Aldrich, Dublin, Ireland).

    Techniques: Injection

    Decreased status epilepticus-induced cortical damage via P2Y 1 antagonism. (A) Representative images (20× lens) and corresponding graph showing a decrease in neuronal damage in the cortex 24 h post-status epilepticus in mice treated with the P2Y 1 antagonist MRS25 (n = 7 Veh, 9 MRS23, and 8 MRS25). (B) Representative images (20x lens) and corresponding graph showing slightly more Fluoro-Jade B (FjB)-positive cells in ipsilateral cortex 24 h post-status epilepticus in mice treated with both minocycline and the P2Y1 antagonist MRS25 (n = 4/group). ** p < 0.01.

    Journal: Frontiers in Pharmacology

    Article Title: Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y 1 Antagonism in Mice

    doi: 10.3389/fphar.2019.01558

    Figure Lengend Snippet: Decreased status epilepticus-induced cortical damage via P2Y 1 antagonism. (A) Representative images (20× lens) and corresponding graph showing a decrease in neuronal damage in the cortex 24 h post-status epilepticus in mice treated with the P2Y 1 antagonist MRS25 (n = 7 Veh, 9 MRS23, and 8 MRS25). (B) Representative images (20x lens) and corresponding graph showing slightly more Fluoro-Jade B (FjB)-positive cells in ipsilateral cortex 24 h post-status epilepticus in mice treated with both minocycline and the P2Y1 antagonist MRS25 (n = 4/group). ** p < 0.01.

    Article Snippet: Membranes were probed with antibodies against P2Y 1 , P2Y 2 , P2Y 4 , P2Y 6 , P2Y 12 , P2Y 13 , and P2Y 14 (Alomone Labs, Hadassah Ein Kerem, Jerusalem, Israel), c-Fos (Santa Cruz, Heidelberg, Germany), and β-actin (Sigma-Aldrich, Dublin, Ireland).

    Techniques: