applied science complete protease inhibitor mixture tablets  (Roche)


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    Structured Review

    Roche applied science complete protease inhibitor mixture tablets
    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic <t>complete</t> medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.
    Applied Science Complete Protease Inhibitor Mixture Tablets, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied science complete protease inhibitor mixture tablets/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    applied science complete protease inhibitor mixture tablets - by Bioz Stars, 2021-06
    86/100 stars

    Images

    1) Product Images from "Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site"

    Article Title: Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M117.775981

    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic complete medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.
    Figure Legend Snippet: The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic complete medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.

    Techniques Used: Activation Assay, Activity Assay

    Related Articles

    Protease Inhibitor:

    Article Title: Streptococcus sanguinis Class Ib Ribonucleotide Reductase
    Article Snippet: .. Cell pellet (18 g) was resuspended in 90 ml of buffer C, containing 1.7 m m FMN and two Complete protease inhibitor mixture tablets (Roche Applied Science). .. The cell suspension was passed twice through a French pressure cell at 14,000 p.s.i. and then centrifuged at 30,000 × g for 20 min. Nucleic acids were precipitated by addition of streptomycin sulfate solution to a final concentration of 1.3% (w/v) with stirring for 15 min. After the solution was spun down at 30,000 × g for 30 min, the supernatant was loaded onto Ni-NTA-agarose column (Qiagen, 1.5 × 3.4 cm, 6 ml) pre-equilibrated with buffer C, and the column was washed with buffer C containing 100 m m NaCl and 20 m m imidazole, pH 7.6, until A 280 of the flow-through was < 0.02.

    Article Title: Munc18a Does Not Alter Fusion Rates Mediated by Neuronal SNAREs, Synaptotagmin, and Complexin *
    Article Snippet: Wild Type Munc18a and Munc18a(S306E/S313E) Wild type Munc18-1a (referred to here as Munc18a) and Munc18a (S306E/S313E) were expressed in E. coli with an N-terminal, TEV protease-cleavable hexahistidine tag from plasmid pPROExHTa. .. Proteins were expressed by the addition of IPTG to late log phase cultures (A 600 ∼0.6–0.8) to 0.5 mm at 20 °C for 16–18 h. Cell pellets from 6 liters of culture were resuspended in 200 ml of Munc18a lysis buffer (20 mm sodium phosphate, pH 8, 300 mm NaCl, 10 mm imidazole), supplemented with 4 EDTA-free Complete Protease Inhibitor Mixture tablets (Roche Applied Science) and 1 mm PMSF and lysed by passing them three times through an Emulsiflex C5 homogenizer (Avestin) at 15,000 p.s.i. three times. .. The lysate was clarified in the Ti45 rotor (Beckman Coulter) at 40,000 rpm for 35 min.

    Article Title: Innate lymphoid cell type 3–derived interleukin-22 boosts lipocalin-2 production in intestinal epithelial cells via synergy between STAT3 and NF-κB
    Article Snippet: .. HT-29 cells were washed with ice-cold PBS and lysed with RIPA buffer containing protease inhibitor mixture cOmplete ULTRA tablets (Roche Applied Science, Basel, Switzerland) and PHOSSTOP phosphatase inhibitors (Roche Applied Science). .. Protein concentrations were determined with the Pierce BCA kit (ThermoFisher Scientific) according to the manufacturer's protocol.

    Article Title: Internalization of the Human Nicotinic Acid Receptor GPR109A Is Regulated by Gi, GRK2, and Arrestin3 *
    Article Snippet: Images were collected using QED camera software and processed with Adobe Photoshop. .. To analyze the knockdown of siRNA-targeted proteins and phosphorylation of ERK1/2, siRNA-transfected or agonist-stimulated HEK-293 cells in a 6-well plate were washed twice with ice-cold PBS and lysed with buffer (20 m m HEPES (pH 7.5), 10 m m EDTA, 150 m m NaCl, 1% Triton X-100, and one tablet of complete protease inhibitor (Roche Applied Science) per 50 ml) at 4 °C on a rocker for 30 min. .. The lysates were centrifuged at 4 °C at 12,000 rpm for 15 min. For examining arrestin3 membrane translocation, HEK-293 cells stably expressing GPR109A were incubated for 8 min with or without 100 μ m niacin, and after washing twice with ice-cold PBS, cells were scraped in PBS containing complete protease inhibitor mixture.

    Article Title: Functional genetic variation in aminopeptidase A (ENPEP): lack of clear association with focal and segmental glomerulosclerosis (FSGS)
    Article Snippet: Transfected COS-1 cells grown on 14mm glass bottom coated microwell culture dishes were visualized for their GFP expression using a blue light FITC filter that detected the excitation peak of 488nm and peak emission of 509nm of the GFP molecule. .. Cells grown on 6-well culture plates were transiently co-transfected with 1μg of each full-length wild-type pDsRed-Monomer-N1 and 1μg of variant pEGFP-N1- ENPEP plasmid DNA, lysed in ice-cold 20mM HEPES, pH 7.0 buffer containing 0.5% Triton X-100 buffer, 2mM EGTA, 4mM EDTA, 30mM sodium fluoride, 40mM b-glycerophosphate, 20mM sodium pyrophosphate, 1mM orthovanadate and a protease inhibitor mixture tablet (Complete, Roche Applied Science). .. The cell lysate was mixed with an equal volume of 4X electrophoresis sample buffer and boiled for 5 minutes.

    Lysis:

    Article Title: Munc18a Does Not Alter Fusion Rates Mediated by Neuronal SNAREs, Synaptotagmin, and Complexin *
    Article Snippet: Wild Type Munc18a and Munc18a(S306E/S313E) Wild type Munc18-1a (referred to here as Munc18a) and Munc18a (S306E/S313E) were expressed in E. coli with an N-terminal, TEV protease-cleavable hexahistidine tag from plasmid pPROExHTa. .. Proteins were expressed by the addition of IPTG to late log phase cultures (A 600 ∼0.6–0.8) to 0.5 mm at 20 °C for 16–18 h. Cell pellets from 6 liters of culture were resuspended in 200 ml of Munc18a lysis buffer (20 mm sodium phosphate, pH 8, 300 mm NaCl, 10 mm imidazole), supplemented with 4 EDTA-free Complete Protease Inhibitor Mixture tablets (Roche Applied Science) and 1 mm PMSF and lysed by passing them three times through an Emulsiflex C5 homogenizer (Avestin) at 15,000 p.s.i. three times. .. The lysate was clarified in the Ti45 rotor (Beckman Coulter) at 40,000 rpm for 35 min.

    Article Title: The NS1 protein of influenza a virus interacts with heat shock protein Hsp90 in human alveolar basal epithelial cells: Implication for virus-induced apoptosis
    Article Snippet: Expression of caspase-9 and caspase-3 The expression of caspase-9 and caspase-3 in the NS1-transfected A549 cells was measured by Western-blot analysis. .. Briefly, the monolayer of cells transfected with pCMV-myc/3NS1 were lysed with ice-cold lysis buffer (150 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40, 1 tablet Complete Mini protein inhibitor mixture/10 ml (Roche Applied Science, Indianapolis, IN) and 0.7 μg/ml pepstatin), and the cell lysates were clarified by centrifugation at 20,000 g for 10 min at 4°C. .. The caspase-9 and caspase-3 activities were determined according to the supplemental protocols of the Caspase-9/Mch6 Colorimetric Assay kit and Caspase-3/CPP32 Colorimetric Protease Assay kit (MBL, Nagoya, Japan), respectively.

    Transfection:

    Article Title: The NS1 protein of influenza a virus interacts with heat shock protein Hsp90 in human alveolar basal epithelial cells: Implication for virus-induced apoptosis
    Article Snippet: Expression of caspase-9 and caspase-3 The expression of caspase-9 and caspase-3 in the NS1-transfected A549 cells was measured by Western-blot analysis. .. Briefly, the monolayer of cells transfected with pCMV-myc/3NS1 were lysed with ice-cold lysis buffer (150 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40, 1 tablet Complete Mini protein inhibitor mixture/10 ml (Roche Applied Science, Indianapolis, IN) and 0.7 μg/ml pepstatin), and the cell lysates were clarified by centrifugation at 20,000 g for 10 min at 4°C. .. The caspase-9 and caspase-3 activities were determined according to the supplemental protocols of the Caspase-9/Mch6 Colorimetric Assay kit and Caspase-3/CPP32 Colorimetric Protease Assay kit (MBL, Nagoya, Japan), respectively.

    Centrifugation:

    Article Title: The NS1 protein of influenza a virus interacts with heat shock protein Hsp90 in human alveolar basal epithelial cells: Implication for virus-induced apoptosis
    Article Snippet: Expression of caspase-9 and caspase-3 The expression of caspase-9 and caspase-3 in the NS1-transfected A549 cells was measured by Western-blot analysis. .. Briefly, the monolayer of cells transfected with pCMV-myc/3NS1 were lysed with ice-cold lysis buffer (150 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM EDTA, 0.5% Nonidet P-40, 1 tablet Complete Mini protein inhibitor mixture/10 ml (Roche Applied Science, Indianapolis, IN) and 0.7 μg/ml pepstatin), and the cell lysates were clarified by centrifugation at 20,000 g for 10 min at 4°C. .. The caspase-9 and caspase-3 activities were determined according to the supplemental protocols of the Caspase-9/Mch6 Colorimetric Assay kit and Caspase-3/CPP32 Colorimetric Protease Assay kit (MBL, Nagoya, Japan), respectively.

    Variant Assay:

    Article Title: Functional genetic variation in aminopeptidase A (ENPEP): lack of clear association with focal and segmental glomerulosclerosis (FSGS)
    Article Snippet: Transfected COS-1 cells grown on 14mm glass bottom coated microwell culture dishes were visualized for their GFP expression using a blue light FITC filter that detected the excitation peak of 488nm and peak emission of 509nm of the GFP molecule. .. Cells grown on 6-well culture plates were transiently co-transfected with 1μg of each full-length wild-type pDsRed-Monomer-N1 and 1μg of variant pEGFP-N1- ENPEP plasmid DNA, lysed in ice-cold 20mM HEPES, pH 7.0 buffer containing 0.5% Triton X-100 buffer, 2mM EGTA, 4mM EDTA, 30mM sodium fluoride, 40mM b-glycerophosphate, 20mM sodium pyrophosphate, 1mM orthovanadate and a protease inhibitor mixture tablet (Complete, Roche Applied Science). .. The cell lysate was mixed with an equal volume of 4X electrophoresis sample buffer and boiled for 5 minutes.

    Plasmid Preparation:

    Article Title: Functional genetic variation in aminopeptidase A (ENPEP): lack of clear association with focal and segmental glomerulosclerosis (FSGS)
    Article Snippet: Transfected COS-1 cells grown on 14mm glass bottom coated microwell culture dishes were visualized for their GFP expression using a blue light FITC filter that detected the excitation peak of 488nm and peak emission of 509nm of the GFP molecule. .. Cells grown on 6-well culture plates were transiently co-transfected with 1μg of each full-length wild-type pDsRed-Monomer-N1 and 1μg of variant pEGFP-N1- ENPEP plasmid DNA, lysed in ice-cold 20mM HEPES, pH 7.0 buffer containing 0.5% Triton X-100 buffer, 2mM EGTA, 4mM EDTA, 30mM sodium fluoride, 40mM b-glycerophosphate, 20mM sodium pyrophosphate, 1mM orthovanadate and a protease inhibitor mixture tablet (Complete, Roche Applied Science). .. The cell lysate was mixed with an equal volume of 4X electrophoresis sample buffer and boiled for 5 minutes.

    Incubation:

    Article Title: La-related Protein 1 (LARP1) Represses Terminal Oligopyrimidine (TOP) mRNA Translation Downstream of mTOR Complex 1 (mTORC1) *
    Article Snippet: Amino acid deprivation experiments were carried out by switching cells to DMEM lacking all amino acids (catalogue no. 319-004-CL, Wisent Inc.) supplemented with dialyzed fetal bovine serum (catalogue no. 26400-036, Gibco Life Technologies, Inc.) for the times indicated (see legends to C and ). .. Cell lysates were prepared by washing cells once with ice-cold PBS followed by incubation with extraction buffer (40 m m HEPES (pH 7.5) at room temperature, 0.3% (w/v) CHAPS, 120 m m NaCl, 1 m m EDTA, 10 m m sodium pyrophosphate, 10 m m β-glycerophosphate, 50 m m sodium fluoride, 1.5 m m sodium orthovanadate, 1 μg/ml RNase A (catalogue no. 10109169001, Roche Applied Science), 0.5 m m DTT, and complete EDTA-free protease inhibitors mixture tablets (catalogue no. 04693 132 001, Roche Applied Science)) for 30 min on ice followed by scraping, as originally described ( ). .. RNase A was omitted from extraction buffer for RNA immunoprecipitation experiments.

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    Roche applied science complete protease inhibitor mixture tablets
    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic <t>complete</t> medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.
    Applied Science Complete Protease Inhibitor Mixture Tablets, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/applied science complete protease inhibitor mixture tablets/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    applied science complete protease inhibitor mixture tablets - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Roche complete protease inhibitor mixture tablet
    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic <t>complete</t> medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.
    Complete Protease Inhibitor Mixture Tablet, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mixture tablet/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mixture tablet - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Roche complete edta free protease inhibitor mixture tablet
    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic <t>complete</t> medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.
    Complete Edta Free Protease Inhibitor Mixture Tablet, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete edta free protease inhibitor mixture tablet/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete edta free protease inhibitor mixture tablet - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

    86
    Roche complete protease inhibitor mixture tablets
    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic <t>complete</t> medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.
    Complete Protease Inhibitor Mixture Tablets, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/complete protease inhibitor mixture tablets/product/Roche
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    complete protease inhibitor mixture tablets - by Bioz Stars, 2021-06
    86/100 stars
      Buy from Supplier

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    The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic complete medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.

    Journal: The Journal of Biological Chemistry

    Article Title: Copper-zinc superoxide dismutase is activated through a sulfenic acid intermediate at a copper ion entry site

    doi: 10.1074/jbc.M117.775981

    Figure Lengend Snippet: The spacing of the D3 C X C Cys residues is critical for Sod1 activation. A , mutations were made in the C X C motif of Ccs1 to alter the spacing to either a CC or a C XX C motif without disrupting downstream residues. B , viability tests were performed by plating cells on synthetic complete medium with or without lysine at 30 or 37 °C. C , Sod1 activity for various Ccs1 mutants was quantified; error bars represent S.D. D , the status of the disulfide bond was visualized by lysing cells in the presence of a PEG-maleimide alkylating agent that selectively reacts with free thiols.

    Article Snippet: Cells were resuspended in 50 m m NaH2 PO4 , 300 m m NaCl, pH 8.0, with Roche Applied Science cOmplete protease inhibitor mixture tablets.

    Techniques: Activation Assay, Activity Assay