apoptotic dna ladder kit  (Boehringer Mannheim)

 
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    Boehringer Mannheim apoptotic dna ladder kit
    Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. <t>Apoptotic</t> cells characterized by condensed <t>DNA</t> were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p
    Apoptotic Dna Ladder Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptotic dna ladder kit/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptotic dna ladder kit - by Bioz Stars, 2021-04
    86/100 stars

    Images

    1) Product Images from "Induction of apoptosis in rat peripheral blood lymphocytes by the anticancer drug CI-994 (acetyldinaline)"

    Article Title: Induction of apoptosis in rat peripheral blood lymphocytes by the anticancer drug CI-994 (acetyldinaline)

    Journal: Journal of Biomedicine and Biotechnology

    doi: 10.1155/S1110724301000146

    Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. Apoptotic cells characterized by condensed DNA were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p
    Figure Legend Snippet: Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. Apoptotic cells characterized by condensed DNA were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p

    Techniques Used: Microscopy, Staining

    2) Product Images from "Mink Cell Focus-Forming Murine Leukemia Virus Infection Induces Apoptosis of Thymic Lymphocytes"

    Article Title: Mink Cell Focus-Forming Murine Leukemia Virus Infection Induces Apoptosis of Thymic Lymphocytes

    Journal: Journal of Virology

    doi:

    DNA fragmentation of thymic lymphocytes. Cellular DNA (1.5 μg) isolated from thymic lymphocytes with the Apoptotic DNA Ladder kit (Boehringer Mannheim) was electrophoresed in each well of a 1.5% agarose gel at 100 V for 1.5 h. DNA was extracted from pooled lymphocytes from thymuses of three mice which were either control (C) or inoculated with MCF13 MLV for 8 weeks (MLV). Thymic cells were placed in culture for 0, 6, 24, and 48 h before DNA extraction. Lane M, 100-bp DNA ladder (Gibco-BRL). Three independent assays which were performed with different pools of thymic lymphocytes yielded similar results.
    Figure Legend Snippet: DNA fragmentation of thymic lymphocytes. Cellular DNA (1.5 μg) isolated from thymic lymphocytes with the Apoptotic DNA Ladder kit (Boehringer Mannheim) was electrophoresed in each well of a 1.5% agarose gel at 100 V for 1.5 h. DNA was extracted from pooled lymphocytes from thymuses of three mice which were either control (C) or inoculated with MCF13 MLV for 8 weeks (MLV). Thymic cells were placed in culture for 0, 6, 24, and 48 h before DNA extraction. Lane M, 100-bp DNA ladder (Gibco-BRL). Three independent assays which were performed with different pools of thymic lymphocytes yielded similar results.

    Techniques Used: Isolation, Agarose Gel Electrophoresis, Mouse Assay, DNA Extraction

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Role of Transmembrane 4 Superfamily (Tm4sf) Proteins Cd9 and Cd81 in Muscle Cell Fusion and Myotube Maintenance
    Article Snippet: For DNA ladder analyses, C2C12 cells were cultured for 6 d in differentiation medium, and then myotubes were detached from mixed myoblast/myotube cultures by treatment with 0.01% trypsin, 0.004% EDTA for 15 min as described previously . .. DNAs were extracted using the Apoptotic DNA Ladder Kit (Boehringer Mannheim) according to the manufacturer's instructions, electrophoresed in 2% agarose gel, and stained with 0.5 μg/ml ethidium bromide. .. Immunoprecipitation C2C12 cells were lysed in lysis buffer (1% Brij 96 or Brij 99, 25 mM Hepes, pH 7.5, 150 mM NaCl, 5 mM MgCl2 , 2 mM PMSF, 10 μg/ml aprotinin, 10 μg/ml leupeptin) for 1 h at 4°C.

    Staining:

    Article Title: Role of Transmembrane 4 Superfamily (Tm4sf) Proteins Cd9 and Cd81 in Muscle Cell Fusion and Myotube Maintenance
    Article Snippet: For DNA ladder analyses, C2C12 cells were cultured for 6 d in differentiation medium, and then myotubes were detached from mixed myoblast/myotube cultures by treatment with 0.01% trypsin, 0.004% EDTA for 15 min as described previously . .. DNAs were extracted using the Apoptotic DNA Ladder Kit (Boehringer Mannheim) according to the manufacturer's instructions, electrophoresed in 2% agarose gel, and stained with 0.5 μg/ml ethidium bromide. .. Immunoprecipitation C2C12 cells were lysed in lysis buffer (1% Brij 96 or Brij 99, 25 mM Hepes, pH 7.5, 150 mM NaCl, 5 mM MgCl2 , 2 mM PMSF, 10 μg/ml aprotinin, 10 μg/ml leupeptin) for 1 h at 4°C.

    other:

    Article Title: Comparison of several techniques for the detection of apoptotic astrocytes in vitro
    Article Snippet: Parallel to these tests, the results obtained with the Jurkat T cells proved the efficacity of the kit: nevertheless, regarding all the results we obtained with this kit (‘Apoptotic DNA ladder kit’) and in such model, the kit seems not to be highly efficient to highlight the DNA ladder described as typical of apoptosis.

    DNA Fragmentation Assay:

    Article Title: Induction of apoptosis in rat peripheral blood lymphocytes by the anticancer drug CI-994 (acetyldinaline)
    Article Snippet: .. DNA fragmentation assay DNA was isolated from lymphocytes using the apoptotic DNA-Ladder kit (Boehringer Mannheim, Indianapolis, IN). .. Isolated DNA was quantitated spectrophotometrically using Softmax Pro 2.4.1 software (Molecular Dynamics, Sunnyvale, CA).

    Isolation:

    Article Title: Induction of apoptosis in rat peripheral blood lymphocytes by the anticancer drug CI-994 (acetyldinaline)
    Article Snippet: .. DNA fragmentation assay DNA was isolated from lymphocytes using the apoptotic DNA-Ladder kit (Boehringer Mannheim, Indianapolis, IN). .. Isolated DNA was quantitated spectrophotometrically using Softmax Pro 2.4.1 software (Molecular Dynamics, Sunnyvale, CA).

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    • apoptotic dna ladder kit  (Boehringer Mannheim)
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    Boehringer Mannheim apoptotic dna ladder kit
    Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. <t>Apoptotic</t> cells characterized by condensed <t>DNA</t> were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p
    Apoptotic Dna Ladder Kit, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptotic dna ladder kit/product/Boehringer Mannheim
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptotic dna ladder kit - by Bioz Stars, 2021-04
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. Apoptotic cells characterized by condensed DNA were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p

    Journal: Journal of Biomedicine and Biotechnology

    Article Title: Induction of apoptosis in rat peripheral blood lymphocytes by the anticancer drug CI-994 (acetyldinaline)

    doi: 10.1155/S1110724301000146

    Figure Lengend Snippet: Apoptosis in rat peripheral blood lymphocytes exposed to CI-994 for 4 or 24 hours. Mitogen (concanavalin A, 0.63 μg/ml) was added to all lymphocyte cultures at time zero. Apoptotic cells characterized by condensed DNA were visualized by fluorescent microscopy and acridine orange/ethidium bromide stain. Data represent the mean ± SE of at least 4 individual experiments. * Significantly different from control using Kruskal-Wallis one way ANOVA on ranked data and Dunn's post hoc test ( p

    Article Snippet: DNA fragmentation assay DNA was isolated from lymphocytes using the apoptotic DNA-Ladder kit (Boehringer Mannheim, Indianapolis, IN).

    Techniques: Microscopy, Staining

    DNA fragmentation of thymic lymphocytes. Cellular DNA (1.5 μg) isolated from thymic lymphocytes with the Apoptotic DNA Ladder kit (Boehringer Mannheim) was electrophoresed in each well of a 1.5% agarose gel at 100 V for 1.5 h. DNA was extracted from pooled lymphocytes from thymuses of three mice which were either control (C) or inoculated with MCF13 MLV for 8 weeks (MLV). Thymic cells were placed in culture for 0, 6, 24, and 48 h before DNA extraction. Lane M, 100-bp DNA ladder (Gibco-BRL). Three independent assays which were performed with different pools of thymic lymphocytes yielded similar results.

    Journal: Journal of Virology

    Article Title: Mink Cell Focus-Forming Murine Leukemia Virus Infection Induces Apoptosis of Thymic Lymphocytes

    doi:

    Figure Lengend Snippet: DNA fragmentation of thymic lymphocytes. Cellular DNA (1.5 μg) isolated from thymic lymphocytes with the Apoptotic DNA Ladder kit (Boehringer Mannheim) was electrophoresed in each well of a 1.5% agarose gel at 100 V for 1.5 h. DNA was extracted from pooled lymphocytes from thymuses of three mice which were either control (C) or inoculated with MCF13 MLV for 8 weeks (MLV). Thymic cells were placed in culture for 0, 6, 24, and 48 h before DNA extraction. Lane M, 100-bp DNA ladder (Gibco-BRL). Three independent assays which were performed with different pools of thymic lymphocytes yielded similar results.

    Article Snippet: Cellular DNA was extracted from 2 × 107 cells using the Apoptotic DNA Ladder kit (Boehringer Mannheim).

    Techniques: Isolation, Agarose Gel Electrophoresis, Mouse Assay, DNA Extraction