Structured Review

Trevigen apoptotic cell system
Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of <t>apoptotic</t> cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.
Apoptotic Cell System, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/apoptotic cell system/product/Trevigen
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
apoptotic cell system - by Bioz Stars, 2021-04
86/100 stars

Images

1) Product Images from "Regulation of Human Immunodeficiency Virus Replication by 2?,5?-Oligoadenylate-Dependent RNase L"

Article Title: Regulation of Human Immunodeficiency Virus Replication by 2?,5?-Oligoadenylate-Dependent RNase L

Journal: Journal of Virology

doi:

Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of apoptotic cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.
Figure Legend Snippet: Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of apoptotic cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.

Techniques Used: Over Expression, Recombinant, Transfection, In Situ

2) Product Images from "The Role of p53 in Bleomycin-Induced DNA Damage in the Lung "

Article Title: The Role of p53 in Bleomycin-Induced DNA Damage in the Lung

Journal: The American Journal of Pathology

doi:

Histological appearance of the lung from a wild-type ( A ) and p53 knockout ( B ) mouse at 3 days after the treatment. In both the wild-type and p53 knockout mice, a few inflammatory cell infiltrates composed of neutrophils and mononuclear cells were observed around bronchi and small blood vessels and within alveolar spaces. No cells exhibiting apoptotic characteristics were seen in either bronchi or alveoli of either group of mice ( insets ). Hematoxylin and eosin; original magnification, ×200 (insets, ×400).
Figure Legend Snippet: Histological appearance of the lung from a wild-type ( A ) and p53 knockout ( B ) mouse at 3 days after the treatment. In both the wild-type and p53 knockout mice, a few inflammatory cell infiltrates composed of neutrophils and mononuclear cells were observed around bronchi and small blood vessels and within alveolar spaces. No cells exhibiting apoptotic characteristics were seen in either bronchi or alveoli of either group of mice ( insets ). Hematoxylin and eosin; original magnification, ×200 (insets, ×400).

Techniques Used: Knock-Out, Mouse Assay

A : DNA electrophoresis of the lung and small intestine from wild-type mice. Lane 1 , marker; Lanes 2–9 , lung (control and at 2 hours to 7 days); Lane 10 , small intestine at 12 hours. DNA laddering was observed in the small intestine at 12 hours after the treatment, but not in the lung at any time point. B : Apoptotic index in the bronchi ( top ), alveoli ( second panel ), small intestinal crypts ( third panel ), and villi ( bottom ) of the wild-type ( solid line ) and p53 knockout ( dashed line ) mice. C and D : Histological appearance of the small intestine of a wild-type ( C ) and p53 knockout ( D ) mouse; left , crypts at 12 hours; right , villi at 3 days after the treatment. Cells with typical apoptotic characteristics were observed not only in the crypts but also in the villi of both groups ( arrows ). Hematoxylin and eosin; original magnification, ×400.
Figure Legend Snippet: A : DNA electrophoresis of the lung and small intestine from wild-type mice. Lane 1 , marker; Lanes 2–9 , lung (control and at 2 hours to 7 days); Lane 10 , small intestine at 12 hours. DNA laddering was observed in the small intestine at 12 hours after the treatment, but not in the lung at any time point. B : Apoptotic index in the bronchi ( top ), alveoli ( second panel ), small intestinal crypts ( third panel ), and villi ( bottom ) of the wild-type ( solid line ) and p53 knockout ( dashed line ) mice. C and D : Histological appearance of the small intestine of a wild-type ( C ) and p53 knockout ( D ) mouse; left , crypts at 12 hours; right , villi at 3 days after the treatment. Cells with typical apoptotic characteristics were observed not only in the crypts but also in the villi of both groups ( arrows ). Hematoxylin and eosin; original magnification, ×400.

Techniques Used: Nucleic Acid Electrophoresis, Mouse Assay, Marker, DNA Laddering, Knock-Out

Related Articles

TUNEL Assay:

Article Title: An In Vitro Model of Skeletal Muscle Volume Regulation
Article Snippet: .. DNA fragmentation characteristic of apoptotic programmed cell death was detected with the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labelling (TUNEL) assay [ ] using the Trevigen apoptotic cell system (TACS) TACS 2 TdT-Fluor Apoptosis Detection Kit (Trevigen, Gaithersburg, MD, USA). ..

Article Title:
Article Snippet: Indirect immunofluorescence staining of Mrp2 and Mrp4 on frozen mouse kidney sections has been reported previously ( ). .. PCNA and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were performed on paraffin-embedded and frozen kidney sections using the Zymed PCNA kit (Invitrogen, Carlsbad, CA) and Trevigen Apoptotic Cell System In Situ Apoptosis Detection Kit (R & D Systems, Minneapolis, MN), respectively. .. PCNA-positive and TUNEL-positive nuclei were counted from three to seven mice per group in three nonoverlapping fields at 40× magnification.

In Situ:

Article Title: Nurr1 is essential for the induction of the dopaminergic phenotype and the survival of ventral mesencephalic late dopaminergic precursor neurons
Article Snippet: .. To identify apoptotic cells, we used the trevigen apoptotic cell system in situ kit (TACS) from Trevigen (Gaithersburg, MD) according to manufacturer’s recommendations. .. The Nurr1 gene was disrupted in mouse embryonic stem cells using the targeting vector shown in Fig. .

Article Title: Regulation of Human Immunodeficiency Virus Replication by 2?,5?-Oligoadenylate-Dependent RNase L
Article Snippet: The blots were stripped with 0.1× SSC for 10 to 15 min at 100°C and probed with a randomly primed, 32 P-labeled DNA probe of 18S rRNA (American Type Culture Collection). .. DNA fragmentation in situ was measured with the Trevigen Apoptotic Cell System by using terminal deoxynucleotide transferase (15 U) in 0.05 M Tris (pH 7.5)–5 mM MgCl2 –0.6 mM β-mercaptoethanesulfonic acid–50 μg of bovine serum albumin–0.25 mM biotinylated nucleotides (dNTPs) at 37°C for 60 min. ..

Article Title:
Article Snippet: Indirect immunofluorescence staining of Mrp2 and Mrp4 on frozen mouse kidney sections has been reported previously ( ). .. PCNA and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were performed on paraffin-embedded and frozen kidney sections using the Zymed PCNA kit (Invitrogen, Carlsbad, CA) and Trevigen Apoptotic Cell System In Situ Apoptosis Detection Kit (R & D Systems, Minneapolis, MN), respectively. .. PCNA-positive and TUNEL-positive nuclei were counted from three to seven mice per group in three nonoverlapping fields at 40× magnification.

Staining:

Article Title:
Article Snippet: Indirect immunofluorescence staining of Mrp2 and Mrp4 on frozen mouse kidney sections has been reported previously ( ). .. PCNA and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining were performed on paraffin-embedded and frozen kidney sections using the Zymed PCNA kit (Invitrogen, Carlsbad, CA) and Trevigen Apoptotic Cell System In Situ Apoptosis Detection Kit (R & D Systems, Minneapolis, MN), respectively. .. PCNA-positive and TUNEL-positive nuclei were counted from three to seven mice per group in three nonoverlapping fields at 40× magnification.

Incubation:

Article Title: Erdr1 Suppresses Murine Melanoma Growth via Regulation of Apoptosis
Article Snippet: Next, the reaction buffer was removed, and specimens were incubated with antibody solution. .. After subsequent incubation with a streptavidin-HRP solution, visualization of the reaction was performed using Trevigen Apoptotic Cell System (TACS) Blue Label solution. ..

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    Trevigen apoptotic cell system
    Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of <t>apoptotic</t> cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.
    Apoptotic Cell System, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptotic cell system/product/Trevigen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptotic cell system - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    86
    Trevigen apoptotic cell system annexin v fitc
    ERA-infected SK-N-SH cells undergo cell death. (I) Confocal microscopy analysis of PS exposed on the outside of the cytoplasmic membrane of ERA-infected SK-N-SH cells (A) Detection of viral G protein on the cytoplasmic membrane of ERA-infected SK-N-SH cells (red). (B) Detection of PS with <t>FITC-annexin</t> V (green). (C) <t>Annexin</t> V and viral G protein labeling are not strictly colocalized. (D and E) Detection of nuclei after propidium iodide staining on infected cells not treated (D) or treated with PFA (E). These images are representative of 69 observations. (II) Nuclear fragmentation in ERA-infected SK-N-SH cells (magnification, ×40). Nonapoptotic strain CVS-infected (panels labeled 1) and proapoptotic strain ERA-infected (panels labeled 2) SK-N-SH cells were stained with Hoechst 33342 (blue) and anti-NC Ab (green). Fragmented nuclei, which are characteristic of apoptosis, were observed in ERA- but not in CVS-infected SK-N-SH cells. These images are representative of 70 observations.
    Apoptotic Cell System Annexin V Fitc, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptotic cell system annexin v fitc/product/Trevigen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptotic cell system annexin v fitc - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of apoptotic cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.

    Journal: Journal of Virology

    Article Title: Regulation of Human Immunodeficiency Virus Replication by 2?,5?-Oligoadenylate-Dependent RNase L

    doi:

    Figure Lengend Snippet: Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of apoptotic cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.

    Article Snippet: DNA fragmentation in situ was measured with the Trevigen Apoptotic Cell System by using terminal deoxynucleotide transferase (15 U) in 0.05 M Tris (pH 7.5)–5 mM MgCl2 –0.6 mM β-mercaptoethanesulfonic acid–50 μg of bovine serum albumin–0.25 mM biotinylated nucleotides (dNTPs) at 37°C for 60 min.

    Techniques: Over Expression, Recombinant, Transfection, In Situ

    Histological appearance of the lung from a wild-type ( A ) and p53 knockout ( B ) mouse at 3 days after the treatment. In both the wild-type and p53 knockout mice, a few inflammatory cell infiltrates composed of neutrophils and mononuclear cells were observed around bronchi and small blood vessels and within alveolar spaces. No cells exhibiting apoptotic characteristics were seen in either bronchi or alveoli of either group of mice ( insets ). Hematoxylin and eosin; original magnification, ×200 (insets, ×400).

    Journal: The American Journal of Pathology

    Article Title: The Role of p53 in Bleomycin-Induced DNA Damage in the Lung

    doi:

    Figure Lengend Snippet: Histological appearance of the lung from a wild-type ( A ) and p53 knockout ( B ) mouse at 3 days after the treatment. In both the wild-type and p53 knockout mice, a few inflammatory cell infiltrates composed of neutrophils and mononuclear cells were observed around bronchi and small blood vessels and within alveolar spaces. No cells exhibiting apoptotic characteristics were seen in either bronchi or alveoli of either group of mice ( insets ). Hematoxylin and eosin; original magnification, ×200 (insets, ×400).

    Article Snippet: DNA double strand breaks and/or apoptotic nucleosomal degradation were detected on paraffin tissue sections by the ISNEL method using the Trevigen apoptotic cell system (Trevigen, Gaithersburg, MD).

    Techniques: Knock-Out, Mouse Assay

    A : DNA electrophoresis of the lung and small intestine from wild-type mice. Lane 1 , marker; Lanes 2–9 , lung (control and at 2 hours to 7 days); Lane 10 , small intestine at 12 hours. DNA laddering was observed in the small intestine at 12 hours after the treatment, but not in the lung at any time point. B : Apoptotic index in the bronchi ( top ), alveoli ( second panel ), small intestinal crypts ( third panel ), and villi ( bottom ) of the wild-type ( solid line ) and p53 knockout ( dashed line ) mice. C and D : Histological appearance of the small intestine of a wild-type ( C ) and p53 knockout ( D ) mouse; left , crypts at 12 hours; right , villi at 3 days after the treatment. Cells with typical apoptotic characteristics were observed not only in the crypts but also in the villi of both groups ( arrows ). Hematoxylin and eosin; original magnification, ×400.

    Journal: The American Journal of Pathology

    Article Title: The Role of p53 in Bleomycin-Induced DNA Damage in the Lung

    doi:

    Figure Lengend Snippet: A : DNA electrophoresis of the lung and small intestine from wild-type mice. Lane 1 , marker; Lanes 2–9 , lung (control and at 2 hours to 7 days); Lane 10 , small intestine at 12 hours. DNA laddering was observed in the small intestine at 12 hours after the treatment, but not in the lung at any time point. B : Apoptotic index in the bronchi ( top ), alveoli ( second panel ), small intestinal crypts ( third panel ), and villi ( bottom ) of the wild-type ( solid line ) and p53 knockout ( dashed line ) mice. C and D : Histological appearance of the small intestine of a wild-type ( C ) and p53 knockout ( D ) mouse; left , crypts at 12 hours; right , villi at 3 days after the treatment. Cells with typical apoptotic characteristics were observed not only in the crypts but also in the villi of both groups ( arrows ). Hematoxylin and eosin; original magnification, ×400.

    Article Snippet: DNA double strand breaks and/or apoptotic nucleosomal degradation were detected on paraffin tissue sections by the ISNEL method using the Trevigen apoptotic cell system (Trevigen, Gaithersburg, MD).

    Techniques: Nucleic Acid Electrophoresis, Mouse Assay, Marker, DNA Laddering, Knock-Out

    ERA-infected SK-N-SH cells undergo cell death. (I) Confocal microscopy analysis of PS exposed on the outside of the cytoplasmic membrane of ERA-infected SK-N-SH cells (A) Detection of viral G protein on the cytoplasmic membrane of ERA-infected SK-N-SH cells (red). (B) Detection of PS with FITC-annexin V (green). (C) Annexin V and viral G protein labeling are not strictly colocalized. (D and E) Detection of nuclei after propidium iodide staining on infected cells not treated (D) or treated with PFA (E). These images are representative of 69 observations. (II) Nuclear fragmentation in ERA-infected SK-N-SH cells (magnification, ×40). Nonapoptotic strain CVS-infected (panels labeled 1) and proapoptotic strain ERA-infected (panels labeled 2) SK-N-SH cells were stained with Hoechst 33342 (blue) and anti-NC Ab (green). Fragmented nuclei, which are characteristic of apoptosis, were observed in ERA- but not in CVS-infected SK-N-SH cells. These images are representative of 70 observations.

    Journal: Journal of Virology

    Article Title: Glycoprotein of Nonpathogenic Rabies Viruses Is a Key Determinant of Human Cell Apoptosis

    doi: 10.1128/JVI.77.19.10537-10547.2003

    Figure Lengend Snippet: ERA-infected SK-N-SH cells undergo cell death. (I) Confocal microscopy analysis of PS exposed on the outside of the cytoplasmic membrane of ERA-infected SK-N-SH cells (A) Detection of viral G protein on the cytoplasmic membrane of ERA-infected SK-N-SH cells (red). (B) Detection of PS with FITC-annexin V (green). (C) Annexin V and viral G protein labeling are not strictly colocalized. (D and E) Detection of nuclei after propidium iodide staining on infected cells not treated (D) or treated with PFA (E). These images are representative of 69 observations. (II) Nuclear fragmentation in ERA-infected SK-N-SH cells (magnification, ×40). Nonapoptotic strain CVS-infected (panels labeled 1) and proapoptotic strain ERA-infected (panels labeled 2) SK-N-SH cells were stained with Hoechst 33342 (blue) and anti-NC Ab (green). Fragmented nuclei, which are characteristic of apoptosis, were observed in ERA- but not in CVS-infected SK-N-SH cells. These images are representative of 70 observations.

    Article Snippet: Trevigen Apoptotic Cell System annexin V-FITC was obtained from R & D Systems Europe (Abingdon, United Kingdom).

    Techniques: Infection, Confocal Microscopy, Labeling, Staining