Journal: Oxidative Medicine and Cellular Longevity

Article Title: The Protective Effects of Trypsin Inhibitor on Hepatic Ischemia-Reperfusion Injury and Liver Graft Survival

doi: 10.1155/2016/1429835

Figure Lengend Snippet: Hepatocyte apoptosis, Caspase-3 , Bcl-2, and Bax gene expression on liver tissue. (a) Hepatocyte apoptosis TUNEL evaluation revealed a significantly reduced number of hepatocellular apoptotic nuclei treated with UTI at 6 hours, especially in the combined treatment group (48.2 ± 1.9 versus 40.4 ± 2.2 versus 26.6 ± 2.9), ∗ P < 0.05. (b) In terms of percentage calculation, three specimens in each group were stained under a 400x light microscope and 4–10 positive fields were observed in each section. The mean ratio of positive cells (brown-yellow stained cells) accounting for total cell count in each group was taken. (c) Caspase-3 and Bax gene expression on liver tissue was reduced, but Bcl-2 was increased significantly in the UTI-treated group, tested by Western blot. (d) The ratios of Bax/Bcl-2 were 1.5 ± 0.3 versus 0.9 ± 0.2 versus 2.5 ± 0.5, 2.5 ± 0.4 versus 1.8 ± 0.2 versus 3.3 ± 0.2, and 2.3 ± 0.3 versus 1.7 ± 0.1 versus 2.9 ± 0.5. Cleaved-caspase-3/beta-actin was 0.4 ± 0.04 versus 0.2 ± 0.02 versus 0.5 ± 0.02, 0.7 ± 0.04 versus 0.5 ± 0.03 versus 0.8 ± 0.03, and 0.5 ± 0.04 versus 0.3 ± 0.04 versus 0.6 ± 0.03 in the LR + UTI, LR + UTI + RT, and LR groups at 1 h, 6 h, and 18 hours after OLTx, respectively. ( ∗ P < 0.05).

Article Snippet: Rat anti-mouse IL-6, TNF- α , and IFN- γ antibodies were from BD Pharmingen (San Diego, CA, USA), rat anti-mouse IL-10 antibody was from Abcam (Cambridge, UK), Bcl-2, caspase-3, and Bax, β -actin were from Cell Signaling Technology, Inc., (Danvers, MA, USA), and the ApopTag Peroxidase In Situ Apoptosis Detection Kit was from the Millipore Corporation (Billerica, MA, USA).

Techniques: Expressing, TUNEL Assay, Staining, Light Microscopy, Cell Counting, Western Blot