apoptag peroxidase in situ apoptosis detection kit  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc apoptag peroxidase in situ apoptosis detection kit
    Hepatocyte <t>apoptosis,</t> Caspase-3 , Bcl-2, and Bax gene expression on liver tissue. (a) Hepatocyte apoptosis TUNEL evaluation revealed a significantly reduced number of hepatocellular apoptotic nuclei treated with UTI at 6 hours, especially in the combined treatment group (48.2 ± 1.9 versus 40.4 ± 2.2 versus 26.6 ± 2.9), ∗ P < 0.05. (b) In terms of percentage calculation, three specimens in each group were stained under a 400x light microscope and 4–10 positive fields were observed in each section. The mean ratio of positive cells (brown-yellow stained cells) accounting for total cell count in each group was taken. (c) Caspase-3 and Bax gene expression on liver tissue was reduced, but Bcl-2 was increased significantly in the UTI-treated group, tested by Western blot. (d) The ratios of Bax/Bcl-2 were 1.5 ± 0.3 versus 0.9 ± 0.2 versus 2.5 ± 0.5, 2.5 ± 0.4 versus 1.8 ± 0.2 versus 3.3 ± 0.2, and 2.3 ± 0.3 versus 1.7 ± 0.1 versus 2.9 ± 0.5. Cleaved-caspase-3/beta-actin was 0.4 ± 0.04 versus 0.2 ± 0.02 versus 0.5 ± 0.02, 0.7 ± 0.04 versus 0.5 ± 0.03 versus 0.8 ± 0.03, and 0.5 ± 0.04 versus 0.3 ± 0.04 versus 0.6 ± 0.03 in the LR + UTI, LR + UTI + RT, and LR groups at 1 h, 6 h, and 18 hours after OLTx, respectively. ( ∗ P < 0.05).
    Apoptag Peroxidase In Situ Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptag peroxidase in situ apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptag peroxidase in situ apoptosis detection kit - by Bioz Stars, 2024-06
    86/100 stars

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    1) Product Images from "The Protective Effects of Trypsin Inhibitor on Hepatic Ischemia-Reperfusion Injury and Liver Graft Survival"

    Article Title: The Protective Effects of Trypsin Inhibitor on Hepatic Ischemia-Reperfusion Injury and Liver Graft Survival

    Journal: Oxidative Medicine and Cellular Longevity

    doi: 10.1155/2016/1429835

    Hepatocyte apoptosis, Caspase-3 , Bcl-2, and Bax gene expression on liver tissue. (a) Hepatocyte apoptosis TUNEL evaluation revealed a significantly reduced number of hepatocellular apoptotic nuclei treated with UTI at 6 hours, especially in the combined treatment group (48.2 ± 1.9 versus 40.4 ± 2.2 versus 26.6 ± 2.9), ∗ P < 0.05. (b) In terms of percentage calculation, three specimens in each group were stained under a 400x light microscope and 4–10 positive fields were observed in each section. The mean ratio of positive cells (brown-yellow stained cells) accounting for total cell count in each group was taken. (c) Caspase-3 and Bax gene expression on liver tissue was reduced, but Bcl-2 was increased significantly in the UTI-treated group, tested by Western blot. (d) The ratios of Bax/Bcl-2 were 1.5 ± 0.3 versus 0.9 ± 0.2 versus 2.5 ± 0.5, 2.5 ± 0.4 versus 1.8 ± 0.2 versus 3.3 ± 0.2, and 2.3 ± 0.3 versus 1.7 ± 0.1 versus 2.9 ± 0.5. Cleaved-caspase-3/beta-actin was 0.4 ± 0.04 versus 0.2 ± 0.02 versus 0.5 ± 0.02, 0.7 ± 0.04 versus 0.5 ± 0.03 versus 0.8 ± 0.03, and 0.5 ± 0.04 versus 0.3 ± 0.04 versus 0.6 ± 0.03 in the LR + UTI, LR + UTI + RT, and LR groups at 1 h, 6 h, and 18 hours after OLTx, respectively. ( ∗ P < 0.05).
    Figure Legend Snippet: Hepatocyte apoptosis, Caspase-3 , Bcl-2, and Bax gene expression on liver tissue. (a) Hepatocyte apoptosis TUNEL evaluation revealed a significantly reduced number of hepatocellular apoptotic nuclei treated with UTI at 6 hours, especially in the combined treatment group (48.2 ± 1.9 versus 40.4 ± 2.2 versus 26.6 ± 2.9), ∗ P < 0.05. (b) In terms of percentage calculation, three specimens in each group were stained under a 400x light microscope and 4–10 positive fields were observed in each section. The mean ratio of positive cells (brown-yellow stained cells) accounting for total cell count in each group was taken. (c) Caspase-3 and Bax gene expression on liver tissue was reduced, but Bcl-2 was increased significantly in the UTI-treated group, tested by Western blot. (d) The ratios of Bax/Bcl-2 were 1.5 ± 0.3 versus 0.9 ± 0.2 versus 2.5 ± 0.5, 2.5 ± 0.4 versus 1.8 ± 0.2 versus 3.3 ± 0.2, and 2.3 ± 0.3 versus 1.7 ± 0.1 versus 2.9 ± 0.5. Cleaved-caspase-3/beta-actin was 0.4 ± 0.04 versus 0.2 ± 0.02 versus 0.5 ± 0.02, 0.7 ± 0.04 versus 0.5 ± 0.03 versus 0.8 ± 0.03, and 0.5 ± 0.04 versus 0.3 ± 0.04 versus 0.6 ± 0.03 in the LR + UTI, LR + UTI + RT, and LR groups at 1 h, 6 h, and 18 hours after OLTx, respectively. ( ∗ P < 0.05).

    Techniques Used: Expressing, TUNEL Assay, Staining, Light Microscopy, Cell Counting, Western Blot

    apoptag peroxidase in situ apoptosis detection kit  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc apoptag peroxidase in situ apoptosis detection kit
    Apoptag Peroxidase In Situ Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptag peroxidase in situ apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptag peroxidase in situ apoptosis detection kit - by Bioz Stars, 2024-06
    92/100 stars

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    apoptag peroxidase in situ apoptosis detection kit  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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  • 92

    Structured Review

    Cell Signaling Technology Inc apoptag peroxidase in situ apoptosis detection kit
    Apoptag Peroxidase In Situ Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptag peroxidase in situ apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptag peroxidase in situ apoptosis detection kit - by Bioz Stars, 2024-06
    92/100 stars

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    apoptag plus peroxidase in situ apoptosis detection kit  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc apoptag plus peroxidase in situ apoptosis detection kit
    Apoptag Plus Peroxidase In Situ Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptag plus peroxidase in situ apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptag plus peroxidase in situ apoptosis detection kit - by Bioz Stars, 2024-06
    86/100 stars

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    Cell Signaling Technology Inc apoptag peroxidase in situ apoptosis detection kit
    Hepatocyte <t>apoptosis,</t> Caspase-3 , Bcl-2, and Bax gene expression on liver tissue. (a) Hepatocyte apoptosis TUNEL evaluation revealed a significantly reduced number of hepatocellular apoptotic nuclei treated with UTI at 6 hours, especially in the combined treatment group (48.2 ± 1.9 versus 40.4 ± 2.2 versus 26.6 ± 2.9), ∗ P < 0.05. (b) In terms of percentage calculation, three specimens in each group were stained under a 400x light microscope and 4–10 positive fields were observed in each section. The mean ratio of positive cells (brown-yellow stained cells) accounting for total cell count in each group was taken. (c) Caspase-3 and Bax gene expression on liver tissue was reduced, but Bcl-2 was increased significantly in the UTI-treated group, tested by Western blot. (d) The ratios of Bax/Bcl-2 were 1.5 ± 0.3 versus 0.9 ± 0.2 versus 2.5 ± 0.5, 2.5 ± 0.4 versus 1.8 ± 0.2 versus 3.3 ± 0.2, and 2.3 ± 0.3 versus 1.7 ± 0.1 versus 2.9 ± 0.5. Cleaved-caspase-3/beta-actin was 0.4 ± 0.04 versus 0.2 ± 0.02 versus 0.5 ± 0.02, 0.7 ± 0.04 versus 0.5 ± 0.03 versus 0.8 ± 0.03, and 0.5 ± 0.04 versus 0.3 ± 0.04 versus 0.6 ± 0.03 in the LR + UTI, LR + UTI + RT, and LR groups at 1 h, 6 h, and 18 hours after OLTx, respectively. ( ∗ P < 0.05).
    Apoptag Peroxidase In Situ Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptag peroxidase in situ apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptag peroxidase in situ apoptosis detection kit - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc apoptag plus peroxidase in situ apoptosis detection kit
    Hepatocyte <t>apoptosis,</t> Caspase-3 , Bcl-2, and Bax gene expression on liver tissue. (a) Hepatocyte apoptosis TUNEL evaluation revealed a significantly reduced number of hepatocellular apoptotic nuclei treated with UTI at 6 hours, especially in the combined treatment group (48.2 ± 1.9 versus 40.4 ± 2.2 versus 26.6 ± 2.9), ∗ P < 0.05. (b) In terms of percentage calculation, three specimens in each group were stained under a 400x light microscope and 4–10 positive fields were observed in each section. The mean ratio of positive cells (brown-yellow stained cells) accounting for total cell count in each group was taken. (c) Caspase-3 and Bax gene expression on liver tissue was reduced, but Bcl-2 was increased significantly in the UTI-treated group, tested by Western blot. (d) The ratios of Bax/Bcl-2 were 1.5 ± 0.3 versus 0.9 ± 0.2 versus 2.5 ± 0.5, 2.5 ± 0.4 versus 1.8 ± 0.2 versus 3.3 ± 0.2, and 2.3 ± 0.3 versus 1.7 ± 0.1 versus 2.9 ± 0.5. Cleaved-caspase-3/beta-actin was 0.4 ± 0.04 versus 0.2 ± 0.02 versus 0.5 ± 0.02, 0.7 ± 0.04 versus 0.5 ± 0.03 versus 0.8 ± 0.03, and 0.5 ± 0.04 versus 0.3 ± 0.04 versus 0.6 ± 0.03 in the LR + UTI, LR + UTI + RT, and LR groups at 1 h, 6 h, and 18 hours after OLTx, respectively. ( ∗ P < 0.05).
    Apoptag Plus Peroxidase In Situ Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptag plus peroxidase in situ apoptosis detection kit/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoptag plus peroxidase in situ apoptosis detection kit - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

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    Hepatocyte apoptosis, Caspase-3 , Bcl-2, and Bax gene expression on liver tissue. (a) Hepatocyte apoptosis TUNEL evaluation revealed a significantly reduced number of hepatocellular apoptotic nuclei treated with UTI at 6 hours, especially in the combined treatment group (48.2 ± 1.9 versus 40.4 ± 2.2 versus 26.6 ± 2.9), ∗ P < 0.05. (b) In terms of percentage calculation, three specimens in each group were stained under a 400x light microscope and 4–10 positive fields were observed in each section. The mean ratio of positive cells (brown-yellow stained cells) accounting for total cell count in each group was taken. (c) Caspase-3 and Bax gene expression on liver tissue was reduced, but Bcl-2 was increased significantly in the UTI-treated group, tested by Western blot. (d) The ratios of Bax/Bcl-2 were 1.5 ± 0.3 versus 0.9 ± 0.2 versus 2.5 ± 0.5, 2.5 ± 0.4 versus 1.8 ± 0.2 versus 3.3 ± 0.2, and 2.3 ± 0.3 versus 1.7 ± 0.1 versus 2.9 ± 0.5. Cleaved-caspase-3/beta-actin was 0.4 ± 0.04 versus 0.2 ± 0.02 versus 0.5 ± 0.02, 0.7 ± 0.04 versus 0.5 ± 0.03 versus 0.8 ± 0.03, and 0.5 ± 0.04 versus 0.3 ± 0.04 versus 0.6 ± 0.03 in the LR + UTI, LR + UTI + RT, and LR groups at 1 h, 6 h, and 18 hours after OLTx, respectively. ( ∗ P < 0.05).

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: The Protective Effects of Trypsin Inhibitor on Hepatic Ischemia-Reperfusion Injury and Liver Graft Survival

    doi: 10.1155/2016/1429835

    Figure Lengend Snippet: Hepatocyte apoptosis, Caspase-3 , Bcl-2, and Bax gene expression on liver tissue. (a) Hepatocyte apoptosis TUNEL evaluation revealed a significantly reduced number of hepatocellular apoptotic nuclei treated with UTI at 6 hours, especially in the combined treatment group (48.2 ± 1.9 versus 40.4 ± 2.2 versus 26.6 ± 2.9), ∗ P < 0.05. (b) In terms of percentage calculation, three specimens in each group were stained under a 400x light microscope and 4–10 positive fields were observed in each section. The mean ratio of positive cells (brown-yellow stained cells) accounting for total cell count in each group was taken. (c) Caspase-3 and Bax gene expression on liver tissue was reduced, but Bcl-2 was increased significantly in the UTI-treated group, tested by Western blot. (d) The ratios of Bax/Bcl-2 were 1.5 ± 0.3 versus 0.9 ± 0.2 versus 2.5 ± 0.5, 2.5 ± 0.4 versus 1.8 ± 0.2 versus 3.3 ± 0.2, and 2.3 ± 0.3 versus 1.7 ± 0.1 versus 2.9 ± 0.5. Cleaved-caspase-3/beta-actin was 0.4 ± 0.04 versus 0.2 ± 0.02 versus 0.5 ± 0.02, 0.7 ± 0.04 versus 0.5 ± 0.03 versus 0.8 ± 0.03, and 0.5 ± 0.04 versus 0.3 ± 0.04 versus 0.6 ± 0.03 in the LR + UTI, LR + UTI + RT, and LR groups at 1 h, 6 h, and 18 hours after OLTx, respectively. ( ∗ P < 0.05).

    Article Snippet: Rat anti-mouse IL-6, TNF- α , and IFN- γ antibodies were from BD Pharmingen (San Diego, CA, USA), rat anti-mouse IL-10 antibody was from Abcam (Cambridge, UK), Bcl-2, caspase-3, and Bax, β -actin were from Cell Signaling Technology, Inc., (Danvers, MA, USA), and the ApopTag Peroxidase In Situ Apoptosis Detection Kit was from the Millipore Corporation (Billerica, MA, USA).

    Techniques: Expressing, TUNEL Assay, Staining, Light Microscopy, Cell Counting, Western Blot