apoi hf  (New England Biolabs)


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    New England Biolabs apoi hf
    Strategy for Producing Drd2 I212F mice. A , Membrane topology of the human D2L receptor, depicting the I 212 position in red. B , Alignment of amino acid sequences from exon 5 of human and mouse D2 dopamine receptor genes, corresponding to the amino acids 178 to 241 in both proteins. Identity between human and mouse amino acid sequences is shown. C , Mouse Drd2 exon 5 nucleotide sequence, including intron/exon 5 junctions, showing <t>the</t> <t>PCR</t> primers (underlined) used for genotyping the mice and the position of the gRNA sequence (double-underlined in blue). Exon 5 sequence is in uppercase and intron sequence in lowercase. In red is shown the position of the wild type amino acid (A-B) and nucleotide (C) that was changed to generate the D2-I 212 F variant. The single stranded oligonucleotide (ssODN) template contained three synonymous changes C-A, T-A and C-A; the WT nucleotide is shown at these positions in blue. Moreover, underlined sequence in red (C) shows the position of <t>ApoI</t> (RAATTY) site, whereas the box shows the location of the introduced RsaI (GTAC) site. Both restriction sites are present in KI but not in WT mice. R means purine (A/G) and Y, pyrimidine (T/C).
    Apoi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoi hf/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoi hf - by Bioz Stars, 2022-07
    94/100 stars

    Images

    1) Product Images from "Gait Abnormalities and Aberrant D2 Receptor Expression and Signaling in a Mouse Model of the Human Pathogenic Mutation DRD2I212F"

    Article Title: Gait Abnormalities and Aberrant D2 Receptor Expression and Signaling in a Mouse Model of the Human Pathogenic Mutation DRD2I212F

    Journal: bioRxiv

    doi: 10.1101/2022.06.09.495548

    Strategy for Producing Drd2 I212F mice. A , Membrane topology of the human D2L receptor, depicting the I 212 position in red. B , Alignment of amino acid sequences from exon 5 of human and mouse D2 dopamine receptor genes, corresponding to the amino acids 178 to 241 in both proteins. Identity between human and mouse amino acid sequences is shown. C , Mouse Drd2 exon 5 nucleotide sequence, including intron/exon 5 junctions, showing the PCR primers (underlined) used for genotyping the mice and the position of the gRNA sequence (double-underlined in blue). Exon 5 sequence is in uppercase and intron sequence in lowercase. In red is shown the position of the wild type amino acid (A-B) and nucleotide (C) that was changed to generate the D2-I 212 F variant. The single stranded oligonucleotide (ssODN) template contained three synonymous changes C-A, T-A and C-A; the WT nucleotide is shown at these positions in blue. Moreover, underlined sequence in red (C) shows the position of ApoI (RAATTY) site, whereas the box shows the location of the introduced RsaI (GTAC) site. Both restriction sites are present in KI but not in WT mice. R means purine (A/G) and Y, pyrimidine (T/C).
    Figure Legend Snippet: Strategy for Producing Drd2 I212F mice. A , Membrane topology of the human D2L receptor, depicting the I 212 position in red. B , Alignment of amino acid sequences from exon 5 of human and mouse D2 dopamine receptor genes, corresponding to the amino acids 178 to 241 in both proteins. Identity between human and mouse amino acid sequences is shown. C , Mouse Drd2 exon 5 nucleotide sequence, including intron/exon 5 junctions, showing the PCR primers (underlined) used for genotyping the mice and the position of the gRNA sequence (double-underlined in blue). Exon 5 sequence is in uppercase and intron sequence in lowercase. In red is shown the position of the wild type amino acid (A-B) and nucleotide (C) that was changed to generate the D2-I 212 F variant. The single stranded oligonucleotide (ssODN) template contained three synonymous changes C-A, T-A and C-A; the WT nucleotide is shown at these positions in blue. Moreover, underlined sequence in red (C) shows the position of ApoI (RAATTY) site, whereas the box shows the location of the introduced RsaI (GTAC) site. Both restriction sites are present in KI but not in WT mice. R means purine (A/G) and Y, pyrimidine (T/C).

    Techniques Used: Mouse Assay, Sequencing, Polymerase Chain Reaction, Genotyping Assay, Variant Assay

    Strategy for Producing Drd2 I212F mice. A , A 1.5% agarose gel stained with ethidium bromide showed examples of PCR products from Drd2 -exon 5 amplified using Exon5-F1/Exon5-RC1 (376 bp, left side) and Exon5-F2/Exon5-RC2 primer pairs (181 bp, right side). B , A representative gel is displayed to show Drd2 +/+ (WT), Drd2 +/I212F (+/-) and Drd2 I212F/I212F (+/+) genotypes. Representative 376-bp PCR amplicons were digested with ApoI-HF and DNA samples were run in a 1.5% agarose gel stained with SYBR Safe DNA Gel Stain (APExBIO; Houston, TX). GeneRuler 1 Kb DNA ladder (Thermo Scientific, Inc; Waltham, MA) was used as molecular weight DNA marker.
    Figure Legend Snippet: Strategy for Producing Drd2 I212F mice. A , A 1.5% agarose gel stained with ethidium bromide showed examples of PCR products from Drd2 -exon 5 amplified using Exon5-F1/Exon5-RC1 (376 bp, left side) and Exon5-F2/Exon5-RC2 primer pairs (181 bp, right side). B , A representative gel is displayed to show Drd2 +/+ (WT), Drd2 +/I212F (+/-) and Drd2 I212F/I212F (+/+) genotypes. Representative 376-bp PCR amplicons were digested with ApoI-HF and DNA samples were run in a 1.5% agarose gel stained with SYBR Safe DNA Gel Stain (APExBIO; Houston, TX). GeneRuler 1 Kb DNA ladder (Thermo Scientific, Inc; Waltham, MA) was used as molecular weight DNA marker.

    Techniques Used: Mouse Assay, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

    2) Product Images from "Gait Abnormalities and Aberrant D2 Receptor Expression and Signaling in a Mouse Model of the Human Pathogenic Mutation DRD2I212F"

    Article Title: Gait Abnormalities and Aberrant D2 Receptor Expression and Signaling in a Mouse Model of the Human Pathogenic Mutation DRD2I212F

    Journal: bioRxiv

    doi: 10.1101/2022.06.09.495548

    Strategy for Producing Drd2 I212F mice. A , Membrane topology of the human D2L receptor, depicting the I 212 position in red. B , Alignment of amino acid sequences from exon 5 of human and mouse D2 dopamine receptor genes, corresponding to the amino acids 178 to 241 in both proteins. Identity between human and mouse amino acid sequences is shown. C , Mouse Drd2 exon 5 nucleotide sequence, including intron/exon 5 junctions, showing the PCR primers (underlined) used for genotyping the mice and the position of the gRNA sequence (double-underlined in blue). Exon 5 sequence is in uppercase and intron sequence in lowercase. In red is shown the position of the wild type amino acid (A-B) and nucleotide (C) that was changed to generate the D2-I 212 F variant. The single stranded oligonucleotide (ssODN) template contained three synonymous changes C-A, T-A and C-A; the WT nucleotide is shown at these positions in blue. Moreover, underlined sequence in red (C) shows the position of ApoI (RAATTY) site, whereas the box shows the location of the introduced RsaI (GTAC) site. Both restriction sites are present in KI but not in WT mice. R means purine (A/G) and Y, pyrimidine (T/C).
    Figure Legend Snippet: Strategy for Producing Drd2 I212F mice. A , Membrane topology of the human D2L receptor, depicting the I 212 position in red. B , Alignment of amino acid sequences from exon 5 of human and mouse D2 dopamine receptor genes, corresponding to the amino acids 178 to 241 in both proteins. Identity between human and mouse amino acid sequences is shown. C , Mouse Drd2 exon 5 nucleotide sequence, including intron/exon 5 junctions, showing the PCR primers (underlined) used for genotyping the mice and the position of the gRNA sequence (double-underlined in blue). Exon 5 sequence is in uppercase and intron sequence in lowercase. In red is shown the position of the wild type amino acid (A-B) and nucleotide (C) that was changed to generate the D2-I 212 F variant. The single stranded oligonucleotide (ssODN) template contained three synonymous changes C-A, T-A and C-A; the WT nucleotide is shown at these positions in blue. Moreover, underlined sequence in red (C) shows the position of ApoI (RAATTY) site, whereas the box shows the location of the introduced RsaI (GTAC) site. Both restriction sites are present in KI but not in WT mice. R means purine (A/G) and Y, pyrimidine (T/C).

    Techniques Used: Mouse Assay, Sequencing, Polymerase Chain Reaction, Genotyping Assay, Variant Assay

    Strategy for Producing Drd2 I212F mice. A , A 1.5% agarose gel stained with ethidium bromide showed examples of PCR products from Drd2 -exon 5 amplified using Exon5-F1/Exon5-RC1 (376 bp, left side) and Exon5-F2/Exon5-RC2 primer pairs (181 bp, right side). B , A representative gel is displayed to show Drd2 +/+ (WT), Drd2 +/I212F (+/-) and Drd2 I212F/I212F (+/+) genotypes. Representative 376-bp PCR amplicons were digested with ApoI-HF and DNA samples were run in a 1.5% agarose gel stained with SYBR Safe DNA Gel Stain (APExBIO; Houston, TX). GeneRuler 1 Kb DNA ladder (Thermo Scientific, Inc; Waltham, MA) was used as molecular weight DNA marker.
    Figure Legend Snippet: Strategy for Producing Drd2 I212F mice. A , A 1.5% agarose gel stained with ethidium bromide showed examples of PCR products from Drd2 -exon 5 amplified using Exon5-F1/Exon5-RC1 (376 bp, left side) and Exon5-F2/Exon5-RC2 primer pairs (181 bp, right side). B , A representative gel is displayed to show Drd2 +/+ (WT), Drd2 +/I212F (+/-) and Drd2 I212F/I212F (+/+) genotypes. Representative 376-bp PCR amplicons were digested with ApoI-HF and DNA samples were run in a 1.5% agarose gel stained with SYBR Safe DNA Gel Stain (APExBIO; Houston, TX). GeneRuler 1 Kb DNA ladder (Thermo Scientific, Inc; Waltham, MA) was used as molecular weight DNA marker.

    Techniques Used: Mouse Assay, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker

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    New England Biolabs apoi hf
    Strategy for Producing Drd2 I212F mice. A , Membrane topology of the human D2L receptor, depicting the I 212 position in red. B , Alignment of amino acid sequences from exon 5 of human and mouse D2 dopamine receptor genes, corresponding to the amino acids 178 to 241 in both proteins. Identity between human and mouse amino acid sequences is shown. C , Mouse Drd2 exon 5 nucleotide sequence, including intron/exon 5 junctions, showing <t>the</t> <t>PCR</t> primers (underlined) used for genotyping the mice and the position of the gRNA sequence (double-underlined in blue). Exon 5 sequence is in uppercase and intron sequence in lowercase. In red is shown the position of the wild type amino acid (A-B) and nucleotide (C) that was changed to generate the D2-I 212 F variant. The single stranded oligonucleotide (ssODN) template contained three synonymous changes C-A, T-A and C-A; the WT nucleotide is shown at these positions in blue. Moreover, underlined sequence in red (C) shows the position of <t>ApoI</t> (RAATTY) site, whereas the box shows the location of the introduced RsaI (GTAC) site. Both restriction sites are present in KI but not in WT mice. R means purine (A/G) and Y, pyrimidine (T/C).
    Apoi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoi hf/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apoi hf - by Bioz Stars, 2022-07
    94/100 stars
      Buy from Supplier

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    Strategy for Producing Drd2 I212F mice. A , Membrane topology of the human D2L receptor, depicting the I 212 position in red. B , Alignment of amino acid sequences from exon 5 of human and mouse D2 dopamine receptor genes, corresponding to the amino acids 178 to 241 in both proteins. Identity between human and mouse amino acid sequences is shown. C , Mouse Drd2 exon 5 nucleotide sequence, including intron/exon 5 junctions, showing the PCR primers (underlined) used for genotyping the mice and the position of the gRNA sequence (double-underlined in blue). Exon 5 sequence is in uppercase and intron sequence in lowercase. In red is shown the position of the wild type amino acid (A-B) and nucleotide (C) that was changed to generate the D2-I 212 F variant. The single stranded oligonucleotide (ssODN) template contained three synonymous changes C-A, T-A and C-A; the WT nucleotide is shown at these positions in blue. Moreover, underlined sequence in red (C) shows the position of ApoI (RAATTY) site, whereas the box shows the location of the introduced RsaI (GTAC) site. Both restriction sites are present in KI but not in WT mice. R means purine (A/G) and Y, pyrimidine (T/C).

    Journal: bioRxiv

    Article Title: Gait Abnormalities and Aberrant D2 Receptor Expression and Signaling in a Mouse Model of the Human Pathogenic Mutation DRD2I212F

    doi: 10.1101/2022.06.09.495548

    Figure Lengend Snippet: Strategy for Producing Drd2 I212F mice. A , Membrane topology of the human D2L receptor, depicting the I 212 position in red. B , Alignment of amino acid sequences from exon 5 of human and mouse D2 dopamine receptor genes, corresponding to the amino acids 178 to 241 in both proteins. Identity between human and mouse amino acid sequences is shown. C , Mouse Drd2 exon 5 nucleotide sequence, including intron/exon 5 junctions, showing the PCR primers (underlined) used for genotyping the mice and the position of the gRNA sequence (double-underlined in blue). Exon 5 sequence is in uppercase and intron sequence in lowercase. In red is shown the position of the wild type amino acid (A-B) and nucleotide (C) that was changed to generate the D2-I 212 F variant. The single stranded oligonucleotide (ssODN) template contained three synonymous changes C-A, T-A and C-A; the WT nucleotide is shown at these positions in blue. Moreover, underlined sequence in red (C) shows the position of ApoI (RAATTY) site, whereas the box shows the location of the introduced RsaI (GTAC) site. Both restriction sites are present in KI but not in WT mice. R means purine (A/G) and Y, pyrimidine (T/C).

    Article Snippet: PCR was performed using the HotStarTaq Master Mix Kit (Qiagen; Germantown Road, MD) under the following conditions: 95°C for 3 min, 35 cycles at 95°C for 15s, 58°C for 15s, 72°C for 30s and a final extension step at 72°C for 5 min. PCR products were digested with RsaI or ApoI-HF (NEB Inc; Ipswich, MA) and were separated on 1.5% agarose gels.

    Techniques: Mouse Assay, Sequencing, Polymerase Chain Reaction, Genotyping Assay, Variant Assay

    Strategy for Producing Drd2 I212F mice. A , A 1.5% agarose gel stained with ethidium bromide showed examples of PCR products from Drd2 -exon 5 amplified using Exon5-F1/Exon5-RC1 (376 bp, left side) and Exon5-F2/Exon5-RC2 primer pairs (181 bp, right side). B , A representative gel is displayed to show Drd2 +/+ (WT), Drd2 +/I212F (+/-) and Drd2 I212F/I212F (+/+) genotypes. Representative 376-bp PCR amplicons were digested with ApoI-HF and DNA samples were run in a 1.5% agarose gel stained with SYBR Safe DNA Gel Stain (APExBIO; Houston, TX). GeneRuler 1 Kb DNA ladder (Thermo Scientific, Inc; Waltham, MA) was used as molecular weight DNA marker.

    Journal: bioRxiv

    Article Title: Gait Abnormalities and Aberrant D2 Receptor Expression and Signaling in a Mouse Model of the Human Pathogenic Mutation DRD2I212F

    doi: 10.1101/2022.06.09.495548

    Figure Lengend Snippet: Strategy for Producing Drd2 I212F mice. A , A 1.5% agarose gel stained with ethidium bromide showed examples of PCR products from Drd2 -exon 5 amplified using Exon5-F1/Exon5-RC1 (376 bp, left side) and Exon5-F2/Exon5-RC2 primer pairs (181 bp, right side). B , A representative gel is displayed to show Drd2 +/+ (WT), Drd2 +/I212F (+/-) and Drd2 I212F/I212F (+/+) genotypes. Representative 376-bp PCR amplicons were digested with ApoI-HF and DNA samples were run in a 1.5% agarose gel stained with SYBR Safe DNA Gel Stain (APExBIO; Houston, TX). GeneRuler 1 Kb DNA ladder (Thermo Scientific, Inc; Waltham, MA) was used as molecular weight DNA marker.

    Article Snippet: PCR was performed using the HotStarTaq Master Mix Kit (Qiagen; Germantown Road, MD) under the following conditions: 95°C for 3 min, 35 cycles at 95°C for 15s, 58°C for 15s, 72°C for 30s and a final extension step at 72°C for 5 min. PCR products were digested with RsaI or ApoI-HF (NEB Inc; Ipswich, MA) and were separated on 1.5% agarose gels.

    Techniques: Mouse Assay, Agarose Gel Electrophoresis, Staining, Polymerase Chain Reaction, Amplification, Molecular Weight, Marker