apo i taq i  (New England Biolabs)


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  • 85
    Name:
    ThermoPol Reaction Buffer Pack
    Description:
    ThermoPol Reaction Buffer Pack 6 0 ml
    Catalog Number:
    b9004s
    Price:
    22
    Size:
    6 0 ml
    Category:
    Buffers
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    Structured Review

    New England Biolabs apo i taq i
    ThermoPol Reaction Buffer Pack
    ThermoPol Reaction Buffer Pack 6 0 ml
    https://www.bioz.com/result/apo i taq i/product/New England Biolabs
    Average 85 stars, based on 16515 article reviews
    Price from $9.99 to $1999.99
    apo i taq i - by Bioz Stars, 2020-09
    85/100 stars

    Images

    1) Product Images from "Characterization and Expression Analysis of a Fiber Differentially Expressed Fasciclin-like Arabinogalactan Protein Gene in Sea Island Cotton Fibers"

    Article Title: Characterization and Expression Analysis of a Fiber Differentially Expressed Fasciclin-like Arabinogalactan Protein Gene in Sea Island Cotton Fibers

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0070185

    Visualized differentially expressed TDFs between Sea Island cotton fibers and Upland cotton fibers in cDNA-AFLP analyses. Lane 1 to 3 show Upland cotton ( G. hirsutum cv. CRI 8) fibers and Lane 4 to 6 show Sea Island cotton ( G . barbadense cv. Pima90-53) fibers. Eco RI/ Mse I and Apo I/ Taq I are the restriction enzyme combinations used in cDNA-AFLP analyses. Arrowheads show the differentially expressed FLA TDFs.
    Figure Legend Snippet: Visualized differentially expressed TDFs between Sea Island cotton fibers and Upland cotton fibers in cDNA-AFLP analyses. Lane 1 to 3 show Upland cotton ( G. hirsutum cv. CRI 8) fibers and Lane 4 to 6 show Sea Island cotton ( G . barbadense cv. Pima90-53) fibers. Eco RI/ Mse I and Apo I/ Taq I are the restriction enzyme combinations used in cDNA-AFLP analyses. Arrowheads show the differentially expressed FLA TDFs.

    Techniques Used: cDNA-AFLP Assay

    2) Product Images from "Characterization and Expression Analysis of a Fiber Differentially Expressed Fasciclin-like Arabinogalactan Protein Gene in Sea Island Cotton Fibers"

    Article Title: Characterization and Expression Analysis of a Fiber Differentially Expressed Fasciclin-like Arabinogalactan Protein Gene in Sea Island Cotton Fibers

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0070185

    Visualized differentially expressed TDFs between Sea Island cotton fibers and Upland cotton fibers in cDNA-AFLP analyses. Lane 1 to 3 show Upland cotton ( G. hirsutum cv. CRI 8) fibers and Lane 4 to 6 show Sea Island cotton ( G . barbadense cv. Pima90-53) fibers. Eco RI/ Mse I and Apo I/ Taq I are the restriction enzyme combinations used in cDNA-AFLP analyses. Arrowheads show the differentially expressed FLA TDFs.
    Figure Legend Snippet: Visualized differentially expressed TDFs between Sea Island cotton fibers and Upland cotton fibers in cDNA-AFLP analyses. Lane 1 to 3 show Upland cotton ( G. hirsutum cv. CRI 8) fibers and Lane 4 to 6 show Sea Island cotton ( G . barbadense cv. Pima90-53) fibers. Eco RI/ Mse I and Apo I/ Taq I are the restriction enzyme combinations used in cDNA-AFLP analyses. Arrowheads show the differentially expressed FLA TDFs.

    Techniques Used: cDNA-AFLP Assay

    Full length cDNA sequence of GbFLA5 and its deduced amino acid residues. Nucleotides underlined indicate the start codon of “ATG”, the effectively restriction site in cDNA-AFLP analysis (GAATTC for Eco RI and Apo I, TTAA for Mse I and TCGA for Taq I), and the stop codon of “TGA”, respectively; three conserved regions (H1, H2 and [YA] H) of smart00554 motif were ordinal characterized by wavy line; AGP-like domains are shown by the dashed line.
    Figure Legend Snippet: Full length cDNA sequence of GbFLA5 and its deduced amino acid residues. Nucleotides underlined indicate the start codon of “ATG”, the effectively restriction site in cDNA-AFLP analysis (GAATTC for Eco RI and Apo I, TTAA for Mse I and TCGA for Taq I), and the stop codon of “TGA”, respectively; three conserved regions (H1, H2 and [YA] H) of smart00554 motif were ordinal characterized by wavy line; AGP-like domains are shown by the dashed line.

    Techniques Used: Sequencing, cDNA-AFLP Assay

    Related Articles

    Irradiation:

    Article Title: Controlled Microwave Heating Accelerates Rolling Circle Amplification
    Article Snippet: .. Effect of microwave irradiation on the temperatures of higher concentrations of buffer components added to RCA reaction mixtures The results shown in suggest that the ThermoPol Buffer was a primary factor leading to an increase in temperature under microwave irradiation. .. To determine whether the ThermoPol Buffer contained a specific thermal component that was selectivity affected by microwave irradiation, we measured the temperatures of each of the four ThermoPol Buffer components ( ).

    Article Title: Controlled Microwave Heating Accelerates Rolling Circle Amplification
    Article Snippet: .. To determine whether the ThermoPol Buffer contained a specific thermal component that was selectivity affected by microwave irradiation, we measured the temperatures of each of the four ThermoPol Buffer components ( ). ..

    other:

    Article Title: Controlled Microwave Heating Accelerates Rolling Circle Amplification
    Article Snippet: Thus, to assess the effect of pH, RCA using the Thermopol-buffer prepared by us (pH 8.80 and 8.34) was performed and was compared with a control RCA using NEB Thermopol-buffer.

    Article Title: Controlled Microwave Heating Accelerates Rolling Circle Amplification
    Article Snippet: The temperature profiles of RCA components by microwave heating suggest that the ionic components of the ThermoPol buffer are selectively heated and that the rate of temperature increase induced by microwave heating depends on the components of a molecule and the concentrations of buffer components.

    Article Title: Controlled Microwave Heating Accelerates Rolling Circle Amplification
    Article Snippet: The RCA components of the Bst DNA polymerase-LF reaction mixture were used for this purpose, because only the ThermoPol buffer of this polymerase is a nonproprietary reagent.

    Concentration Assay:

    Article Title: Controlled Microwave Heating Accelerates Rolling Circle Amplification
    Article Snippet: .. However, a substantial change in concentration of the ThermoPol buffer is impossible because enzymatic reactions have an optimum concentration. .. We focused on the four components of ThermoPol buffer.

    Article Title: Controlled Microwave Heating Accelerates Rolling Circle Amplification
    Article Snippet: .. RCA mixtures each containing one component in 4-fold excess of its standard concentration in ThermoPol Buffer (NEB) were prepared as follows: 1 μL of each component (3 M Tris–HCl [pH 8.8], 1.5 M KCl, 1.5 M (NH4 )2 SO4 , and 0.3 M MgSO4 ) was added to an RCA mixture containing a dNTP mixture (2.5 mM each), two primers (10 pmol), circular template–primer complex (50 ng), Bst -LF, and ThermoPol Buffer (20 mM Tris–HCl, 10 mM (NH4 )2 SO4 , 10 mM KCl, 2 mM MgSO4 , and 0.1% Triton X-100). .. Electrophoresis RCA products were electrophoresed in 1.5% agarose gels and visualized using ethidium bromide.

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  • 85
    New England Biolabs apo i taq i
    Visualized differentially expressed TDFs between Sea Island cotton fibers and Upland cotton fibers in cDNA-AFLP analyses. Lane 1 to 3 show Upland cotton ( G. hirsutum cv. CRI 8) fibers and Lane 4 to 6 show Sea Island cotton ( G . barbadense cv. Pima90-53) fibers. Eco RI/ Mse I and Apo I/ <t>Taq</t> I are the restriction enzyme combinations used in cDNA-AFLP analyses. Arrowheads show the differentially expressed FLA TDFs.
    Apo I Taq I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apo i taq i/product/New England Biolabs
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apo i taq i - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Visualized differentially expressed TDFs between Sea Island cotton fibers and Upland cotton fibers in cDNA-AFLP analyses. Lane 1 to 3 show Upland cotton ( G. hirsutum cv. CRI 8) fibers and Lane 4 to 6 show Sea Island cotton ( G . barbadense cv. Pima90-53) fibers. Eco RI/ Mse I and Apo I/ Taq I are the restriction enzyme combinations used in cDNA-AFLP analyses. Arrowheads show the differentially expressed FLA TDFs.

    Journal: PLoS ONE

    Article Title: Characterization and Expression Analysis of a Fiber Differentially Expressed Fasciclin-like Arabinogalactan Protein Gene in Sea Island Cotton Fibers

    doi: 10.1371/journal.pone.0070185

    Figure Lengend Snippet: Visualized differentially expressed TDFs between Sea Island cotton fibers and Upland cotton fibers in cDNA-AFLP analyses. Lane 1 to 3 show Upland cotton ( G. hirsutum cv. CRI 8) fibers and Lane 4 to 6 show Sea Island cotton ( G . barbadense cv. Pima90-53) fibers. Eco RI/ Mse I and Apo I/ Taq I are the restriction enzyme combinations used in cDNA-AFLP analyses. Arrowheads show the differentially expressed FLA TDFs.

    Article Snippet: Two combinations of restriction enzyme of Mse I/Eco RI and Apo I/Taq I (New England Biolabs, USA) were used in cDNA-AFLP analysis.

    Techniques: cDNA-AFLP Assay