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Becton Dickinson apc conjugated anti cd34 antibody
Sample of selected flow cytometric plots for identification of circulating endothelial progenitor cells depending on pre-treatment counts in invasive breast cancer cases and in a healthy individual. Charts 1 and 1a demonstrate a profile of a subject free of breast cancer (control) with circulating endothelial progenitor cells (EPCs) count of 0.30 cells/µL. Charts 2 and 2a show an invasive breast cancer (IBrC) patient with T1a, N0, M0, grade = 2, tumour diameter = 0.5 cm, ER/PR+ and HER2-Ki-67 = 15%, a baseline number of circulating EPCs was 37.99 cells/µL. A patient after 40 months of follow-up is still alive. Charts 3 and 3a demonstrate an IBrC subject with T2, N0, M0, grade = 3, tumour diameter = 2.5 cm, ER+, PR−, HER2−; Ki-67 = 45%, a pre-treatment number of circulating EPCs was 4.32 cells/µL. A patient passed away due to lung, liver, and bones metastases. Quantification of circulating EPCs was made in a peripheral blood mononuclear cell (PBMC) fraction. A gating strategy for the identification of circulating EPCs by applying CD45-PerCP-Cy5.5, CD31-FITC (R2: CD45–/CD31+ gating to exclude lymphocytes), <t>CD34-APC,</t> and CD133-PE (R3: CD133+/CD34+ gating identifies endothelial progenitor cells) conjugated antibodies was used. The CD45 population was gated on the overall lymphocyte + monocyte cells in the PBMC. A detailed flow cytometry plots presentation was published in our previous study [ 9 ].
Apc Conjugated Anti Cd34 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Low Pre-Treatment Count of Circulating Endothelial Progenitors as a Prognostic Biomarker of the High Risk of Breast Cancer Recurrence"

Article Title: Low Pre-Treatment Count of Circulating Endothelial Progenitors as a Prognostic Biomarker of the High Risk of Breast Cancer Recurrence

Journal: Journal of Clinical Medicine

doi: 10.3390/jcm8111984

Sample of selected flow cytometric plots for identification of circulating endothelial progenitor cells depending on pre-treatment counts in invasive breast cancer cases and in a healthy individual. Charts 1 and 1a demonstrate a profile of a subject free of breast cancer (control) with circulating endothelial progenitor cells (EPCs) count of 0.30 cells/µL. Charts 2 and 2a show an invasive breast cancer (IBrC) patient with T1a, N0, M0, grade = 2, tumour diameter = 0.5 cm, ER/PR+ and HER2-Ki-67 = 15%, a baseline number of circulating EPCs was 37.99 cells/µL. A patient after 40 months of follow-up is still alive. Charts 3 and 3a demonstrate an IBrC subject with T2, N0, M0, grade = 3, tumour diameter = 2.5 cm, ER+, PR−, HER2−; Ki-67 = 45%, a pre-treatment number of circulating EPCs was 4.32 cells/µL. A patient passed away due to lung, liver, and bones metastases. Quantification of circulating EPCs was made in a peripheral blood mononuclear cell (PBMC) fraction. A gating strategy for the identification of circulating EPCs by applying CD45-PerCP-Cy5.5, CD31-FITC (R2: CD45–/CD31+ gating to exclude lymphocytes), CD34-APC, and CD133-PE (R3: CD133+/CD34+ gating identifies endothelial progenitor cells) conjugated antibodies was used. The CD45 population was gated on the overall lymphocyte + monocyte cells in the PBMC. A detailed flow cytometry plots presentation was published in our previous study [ 9 ].
Figure Legend Snippet: Sample of selected flow cytometric plots for identification of circulating endothelial progenitor cells depending on pre-treatment counts in invasive breast cancer cases and in a healthy individual. Charts 1 and 1a demonstrate a profile of a subject free of breast cancer (control) with circulating endothelial progenitor cells (EPCs) count of 0.30 cells/µL. Charts 2 and 2a show an invasive breast cancer (IBrC) patient with T1a, N0, M0, grade = 2, tumour diameter = 0.5 cm, ER/PR+ and HER2-Ki-67 = 15%, a baseline number of circulating EPCs was 37.99 cells/µL. A patient after 40 months of follow-up is still alive. Charts 3 and 3a demonstrate an IBrC subject with T2, N0, M0, grade = 3, tumour diameter = 2.5 cm, ER+, PR−, HER2−; Ki-67 = 45%, a pre-treatment number of circulating EPCs was 4.32 cells/µL. A patient passed away due to lung, liver, and bones metastases. Quantification of circulating EPCs was made in a peripheral blood mononuclear cell (PBMC) fraction. A gating strategy for the identification of circulating EPCs by applying CD45-PerCP-Cy5.5, CD31-FITC (R2: CD45–/CD31+ gating to exclude lymphocytes), CD34-APC, and CD133-PE (R3: CD133+/CD34+ gating identifies endothelial progenitor cells) conjugated antibodies was used. The CD45 population was gated on the overall lymphocyte + monocyte cells in the PBMC. A detailed flow cytometry plots presentation was published in our previous study [ 9 ].

Techniques Used: Flow Cytometry, Cytometry

Related Articles

Staining:

Article Title: Engraftment of chronic myelomonocytic leukemia cells in immunocompromised mice supports disease dependency on cytokines
Article Snippet: Mice were euthanatized at 10 to 16 weeks postengraftment. .. Cells from blood, BM, and spleen were stained with the following monoclonal antibodies: allophycocynin (APC)/Cy7 conjugated rat anti-mouse CD45 (Beckman Coulter), phycoerythrin (PE)/Cy7-conjugated mouse anti-human CD45, human specific PE-conjugated anti-CD19, PerCP/Cy5.5-conjugated anti-CD33, Pacific blue-conjugated anti-CD14, Pacific orange-conjugated anti-CD15, APC-conjugated anti-CD34 and fluorescein isothiocyanate–conjugated anti-CD3 (all from BD Pharmingen). .. Stained cells were analyzed on a FACScanto II cytometry (BD Biosciences).

Article Title: C/EBPβ promotes BCR–ABL-mediated myeloid expansion and leukemic stem cell exhaustion
Article Snippet: As lineage markers for human bone marrow mononuclear cells, phycoerythrin (PE)-Cy5-conjugated anti-CD235, biotin-conjugated anti-CD3, CD4, CD8, CD11b, CD14, CD19, CD20 (eBioscience, San Diego, CA, USA) and anti-CD56 antibodies (Bio Legend, San Diego, CA, USA) were used and followed by staining with streptavidin-PE-Cy5. .. Cells were further stained with APC (allophycocyanin)-conjugated anti-CD34 (8G12), PE-conjugated anti-CD38 (HIT2), fluorescein isothiocyanate-conjugated anti-CD45RA (HI100) (all from BD Pharmingen, San Diego, CA, USA) and PE-Cy7-conjugated anti-CD123 antibodies (eBioscience) for definition of HSCs and myeloid progenitors. .. For the staining of mouse cells, PerCP-Cy5.5-conjugated anti-CD3, CD4, CD11b, B220 and Ter119 antibodies, and PE-Cy5.5-conjugated anti-CD8 and Gr-1 antibodies (all from eBioscience) were used as lineage markers.

Article Title: Loss of Ezh2 synergizes with JAK2-V617F in initiating myeloproliferative neoplasms and promoting myelofibrosis
Article Snippet: In brief, c-Kit–positive cells were isolated using PE-conjugated anti–c-Kit antibody and anti-PE microbeads with LS column (Miltenyi Biotec). .. The c-Kit–positive cells were further stained with lineage-marker cocktail followed by PE-Cy7–conjugated anti-CD16/32 (BioLegend), APC-conjugated anti-CD34 (BD), streptavidin-APC-Cy7, and BV421-conjugated anti–Sca-1 antibodies. .. Cell sorting was performed by Influx (BD), and results were analyzed with FlowJo software (Tree Star).

Article Title: TNFSF15 facilitates human umbilical cord blood haematopoietic stem cell expansion by activating Notch signal pathway, et al. TNFSF15 facilitates human umbilical cord blood haematopoietic stem cell expansion by activating Notch signal pathway
Article Snippet: The number of effect wells with expanded cells and cell number in the presence or absence of TNFSF15 for 7 days was scored under microscope. .. On day 14, wells with more than 200 cells were stained with APC‐conjugated anti‐CD34 and PE‐conjugated anti‐CD49f antibodies for 30 minutes at room temperature in dark. ..

Labeling:

Article Title: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes
Article Snippet: In cohort 2, nucleated cells were analyzed after red cell lysis. .. They were labeled with combinations of; PERCP-conjugated anti-CD45 (clone 2D1); PE-conjugated anti-CD38 (HB7), APC-conjugated anti-CD34 (8G12), PERCP-conjugated anti-CD34 (8G12), PE-conjugated isotype control (CD56-MY31), APC-conjugated anti-CD33 (P67.6), PE-conjugated anti-CD117 (10452) (Becton Dickinson, San Jose, CA). .. In both cohorts cells were analyzed on a FACSCalibur flow cytometer (Becton Dickinson, San José, CA, USA).

Incubation:

Article Title: Satellite Cells and Markers of Muscle Regeneration during Unloading and Reloading: Effects of Treatment with Resveratrol and Curcumin
Article Snippet: Cells were resuspended in 1 × 106 cell/100 µL FACS-specific buffer. .. All the cells were incubated with antibodies used to specifically identify the satellite cells: Phycoerythrin (PE)-conjugated anti-alpha-7 integrin (Ablab, Vancouver, Canada), a heterodimeric integral membrane protein critical for the modulation of cell–matrix interactions, and allophycocyanin (APC)-conjugated anti-CD34 (BD Pharmigen, San Jose, CA, USA), as a cell surface sialomucin (a mucopolysaccharide molecule containing sialic acid) with reported anti-adhesive, motile, and pre-proliferative properties for 30 min. Additionally, the cells from all study groups of mice were incubated at the same time (30 min) with specific antibodies to exclude other cell type populations that might also have been present in the muscle extracts, namely endothelial cells, leukocytes and hematopoietic cells: PE-cyanine7 (Cy7)-conjugated anti-CD31 (Biolegend, San Diego, CA, USA), a marker of endothelial cells, both anti-CD11b (Biolegend) and anti-CD45 (Biolegend) as markers of leukocytes, and anti-Sca-1 (Biolegend), a marker of hematopoietic stem cells. ..

Mouse Assay:

Article Title: Satellite Cells and Markers of Muscle Regeneration during Unloading and Reloading: Effects of Treatment with Resveratrol and Curcumin
Article Snippet: Cells were resuspended in 1 × 106 cell/100 µL FACS-specific buffer. .. All the cells were incubated with antibodies used to specifically identify the satellite cells: Phycoerythrin (PE)-conjugated anti-alpha-7 integrin (Ablab, Vancouver, Canada), a heterodimeric integral membrane protein critical for the modulation of cell–matrix interactions, and allophycocyanin (APC)-conjugated anti-CD34 (BD Pharmigen, San Jose, CA, USA), as a cell surface sialomucin (a mucopolysaccharide molecule containing sialic acid) with reported anti-adhesive, motile, and pre-proliferative properties for 30 min. Additionally, the cells from all study groups of mice were incubated at the same time (30 min) with specific antibodies to exclude other cell type populations that might also have been present in the muscle extracts, namely endothelial cells, leukocytes and hematopoietic cells: PE-cyanine7 (Cy7)-conjugated anti-CD31 (Biolegend, San Diego, CA, USA), a marker of endothelial cells, both anti-CD11b (Biolegend) and anti-CD45 (Biolegend) as markers of leukocytes, and anti-Sca-1 (Biolegend), a marker of hematopoietic stem cells. ..

Marker:

Article Title: Satellite Cells and Markers of Muscle Regeneration during Unloading and Reloading: Effects of Treatment with Resveratrol and Curcumin
Article Snippet: Cells were resuspended in 1 × 106 cell/100 µL FACS-specific buffer. .. All the cells were incubated with antibodies used to specifically identify the satellite cells: Phycoerythrin (PE)-conjugated anti-alpha-7 integrin (Ablab, Vancouver, Canada), a heterodimeric integral membrane protein critical for the modulation of cell–matrix interactions, and allophycocyanin (APC)-conjugated anti-CD34 (BD Pharmigen, San Jose, CA, USA), as a cell surface sialomucin (a mucopolysaccharide molecule containing sialic acid) with reported anti-adhesive, motile, and pre-proliferative properties for 30 min. Additionally, the cells from all study groups of mice were incubated at the same time (30 min) with specific antibodies to exclude other cell type populations that might also have been present in the muscle extracts, namely endothelial cells, leukocytes and hematopoietic cells: PE-cyanine7 (Cy7)-conjugated anti-CD31 (Biolegend, San Diego, CA, USA), a marker of endothelial cells, both anti-CD11b (Biolegend) and anti-CD45 (Biolegend) as markers of leukocytes, and anti-Sca-1 (Biolegend), a marker of hematopoietic stem cells. ..

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    Becton Dickinson apc conjugated anti human cd34 mab
    Representative FACS profiles of CB-derived 18Lin − CD45 + <t>CD34</t> +/− CD133 +/− cells. ( a ) The FSC/SSC profile of immunomagnetically separated Lin − cells. The R1 gate was set on the blast–lymphocyte window. ( b ) The R2 gate was set on the 18Lin − living cells. ( c ) The 18Lin − living cells (R2) were subdivided into two fractions: 18Lin − CD45 + CD34 + (R3) and CD34 − (R4) cells, according to their expression levels of CD34. The definitions of CD34 +/− cells are as follows: the CD34 + fraction contains cells expressing maximum <t>APC</t> fluorescence intensity (FI) to 5% the level of FI. The CD34 − level of FI was determined based on the Fluorescence Minus One controls. ( d ) The 18Lin − CD34 + cells residing in the R3 gate were further subdivided into two fractions: 18Lin − CD45 + CD133 + (R5) and CD133 − (R6) cells, according to their expression levels of CD133. The definitions of CD133 +/− cells are as follows: the CD133 + fraction contains cells expressing maximum PE FI to 15% the level of FI and the CD133 − level of FI was determined based on the Fluorescence Minus One controls. ( e ) The R4-gated cells were further subdivided into two fractions: 18Lin − CD45 + CD34 − CD133 + (R7) and CD133 − (R8) cells. The definitions of CD133 +/− cells are the same as described above.
    Apc Conjugated Anti Human Cd34 Mab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apc conjugated anti human cd34 mab/product/Becton Dickinson
    Average 99 stars, based on 1 article reviews
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    95
    Becton Dickinson alexa fluor 647 conjugated anti mouse cd34
    DJ001 promotes human HSC regeneration following irradiation. a At left, representative flow cytometric analysis <t>of</t> CD34 + CD38 - cells at 36 h after 300 cGy irradiation of BM CD34 + cells and culture with media ± 1 µg/mL DJ001. At right, mean percentages and numbers of CD34 + CD38 − cells in each group (Day 0, n = 6; media, DJ001, n = 10). One-way ANOVA with Tukey’s multiple comparison test. b Numbers of CFU-GEMMs within cultures of CD34 + cells at 72 h after 300 cGy and culture with media ± DJ001 ( n = 6). c At left, representative flow cytometric analysis of Annexin V/7AAD staining of CD34 + cells at 24 h after 300 cGy and culture with media ± DJ001 ± EHT1864. At right, percentages of live and apoptotic CD34 + CD38 − cells in each condition (media and DJ001, n = 10; DJ001 + EHT1864, n = 5). One-way ANOVA with Tukey’s multiple comparison test. d Fold changes (2 −ΔΔCt ) in BCL2L1 and MCL-1 gene expression in CD34 + CD38 − cells at 12 h after 300 cGy in media ± DJ001 ( n = 3). Gene transcript changes were normalized to GAPDH and media treatment. Two-way ANOVA with Sidak’s multiple comparison test. e Schematic representation of NSG mice transplantation assay using the progeny of human BM CD34 + cells irradiated with 300 cGy and treated with or without DJ001 × 36 h. f Representative flow cytometric analysis of human CD45 + cells, human CD34 + cells, human CD19 + B cells, human CD33 + myeloid cells, and human CD3 + T cells engraftment in the BM of NSG mice at 12 weeks post transplantation. g Percent engraftment of human hematopoietic cell subsets in the BM of NSG mice at 12 weeks post transplantation ( n = 11–12/group). Source data are provided as Source Data File. * P
    Alexa Fluor 647 Conjugated Anti Mouse Cd34, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 conjugated anti mouse cd34/product/Becton Dickinson
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Becton Dickinson apc mouse anti human cd34
    Mesenchymal stem cell (MSC) characterization in normoxic and hypoxic conditions. a Positive MSC markers (CD105, CD73, and CD90) and b negative MSC markers (CD,14, <t>CD34,</t> CD45)
    Apc Mouse Anti Human Cd34, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apc mouse anti human cd34/product/Becton Dickinson
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apc mouse anti human cd34 - by Bioz Stars, 2021-05
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    Representative FACS profiles of CB-derived 18Lin − CD45 + CD34 +/− CD133 +/− cells. ( a ) The FSC/SSC profile of immunomagnetically separated Lin − cells. The R1 gate was set on the blast–lymphocyte window. ( b ) The R2 gate was set on the 18Lin − living cells. ( c ) The 18Lin − living cells (R2) were subdivided into two fractions: 18Lin − CD45 + CD34 + (R3) and CD34 − (R4) cells, according to their expression levels of CD34. The definitions of CD34 +/− cells are as follows: the CD34 + fraction contains cells expressing maximum APC fluorescence intensity (FI) to 5% the level of FI. The CD34 − level of FI was determined based on the Fluorescence Minus One controls. ( d ) The 18Lin − CD34 + cells residing in the R3 gate were further subdivided into two fractions: 18Lin − CD45 + CD133 + (R5) and CD133 − (R6) cells, according to their expression levels of CD133. The definitions of CD133 +/− cells are as follows: the CD133 + fraction contains cells expressing maximum PE FI to 15% the level of FI and the CD133 − level of FI was determined based on the Fluorescence Minus One controls. ( e ) The R4-gated cells were further subdivided into two fractions: 18Lin − CD45 + CD34 − CD133 + (R7) and CD133 − (R8) cells. The definitions of CD133 +/− cells are the same as described above.

    Journal: Leukemia

    Article Title: CD133 is a positive marker for a distinct class of primitive human cord blood-derived CD34-negative hematopoietic stem cells

    doi: 10.1038/leu.2013.326

    Figure Lengend Snippet: Representative FACS profiles of CB-derived 18Lin − CD45 + CD34 +/− CD133 +/− cells. ( a ) The FSC/SSC profile of immunomagnetically separated Lin − cells. The R1 gate was set on the blast–lymphocyte window. ( b ) The R2 gate was set on the 18Lin − living cells. ( c ) The 18Lin − living cells (R2) were subdivided into two fractions: 18Lin − CD45 + CD34 + (R3) and CD34 − (R4) cells, according to their expression levels of CD34. The definitions of CD34 +/− cells are as follows: the CD34 + fraction contains cells expressing maximum APC fluorescence intensity (FI) to 5% the level of FI. The CD34 − level of FI was determined based on the Fluorescence Minus One controls. ( d ) The 18Lin − CD34 + cells residing in the R3 gate were further subdivided into two fractions: 18Lin − CD45 + CD133 + (R5) and CD133 − (R6) cells, according to their expression levels of CD133. The definitions of CD133 +/− cells are as follows: the CD133 + fraction contains cells expressing maximum PE FI to 15% the level of FI and the CD133 − level of FI was determined based on the Fluorescence Minus One controls. ( e ) The R4-gated cells were further subdivided into two fractions: 18Lin − CD45 + CD34 − CD133 + (R7) and CD133 − (R8) cells. The definitions of CD133 +/− cells are the same as described above.

    Article Snippet: The cells were also stained with a PE-Cy7-conjugated anti-mouse CD45.1 mAb (Beckman Coulter); fluorescein isothiocyanate-conjugated anti-human CD19 (eBioscience), CD11b (Beckman Coulter) and CD235a (DAKO) mAbs; PE-conjugated anti-human CD33, CD14 and CD41 (Beckman Coulter); and an APC-conjugated anti-human CD34 mAb (BD Biosciences) for the detection of human stem/progenitor, B-lymphoid and myeloid hematopoietic cells.

    Techniques: FACS, Derivative Assay, Expressing, Fluorescence

    DJ001 promotes human HSC regeneration following irradiation. a At left, representative flow cytometric analysis of CD34 + CD38 - cells at 36 h after 300 cGy irradiation of BM CD34 + cells and culture with media ± 1 µg/mL DJ001. At right, mean percentages and numbers of CD34 + CD38 − cells in each group (Day 0, n = 6; media, DJ001, n = 10). One-way ANOVA with Tukey’s multiple comparison test. b Numbers of CFU-GEMMs within cultures of CD34 + cells at 72 h after 300 cGy and culture with media ± DJ001 ( n = 6). c At left, representative flow cytometric analysis of Annexin V/7AAD staining of CD34 + cells at 24 h after 300 cGy and culture with media ± DJ001 ± EHT1864. At right, percentages of live and apoptotic CD34 + CD38 − cells in each condition (media and DJ001, n = 10; DJ001 + EHT1864, n = 5). One-way ANOVA with Tukey’s multiple comparison test. d Fold changes (2 −ΔΔCt ) in BCL2L1 and MCL-1 gene expression in CD34 + CD38 − cells at 12 h after 300 cGy in media ± DJ001 ( n = 3). Gene transcript changes were normalized to GAPDH and media treatment. Two-way ANOVA with Sidak’s multiple comparison test. e Schematic representation of NSG mice transplantation assay using the progeny of human BM CD34 + cells irradiated with 300 cGy and treated with or without DJ001 × 36 h. f Representative flow cytometric analysis of human CD45 + cells, human CD34 + cells, human CD19 + B cells, human CD33 + myeloid cells, and human CD3 + T cells engraftment in the BM of NSG mice at 12 weeks post transplantation. g Percent engraftment of human hematopoietic cell subsets in the BM of NSG mice at 12 weeks post transplantation ( n = 11–12/group). Source data are provided as Source Data File. * P

    Journal: Nature Communications

    Article Title: PTPσ inhibitors promote hematopoietic stem cell regeneration

    doi: 10.1038/s41467-019-11490-5

    Figure Lengend Snippet: DJ001 promotes human HSC regeneration following irradiation. a At left, representative flow cytometric analysis of CD34 + CD38 - cells at 36 h after 300 cGy irradiation of BM CD34 + cells and culture with media ± 1 µg/mL DJ001. At right, mean percentages and numbers of CD34 + CD38 − cells in each group (Day 0, n = 6; media, DJ001, n = 10). One-way ANOVA with Tukey’s multiple comparison test. b Numbers of CFU-GEMMs within cultures of CD34 + cells at 72 h after 300 cGy and culture with media ± DJ001 ( n = 6). c At left, representative flow cytometric analysis of Annexin V/7AAD staining of CD34 + cells at 24 h after 300 cGy and culture with media ± DJ001 ± EHT1864. At right, percentages of live and apoptotic CD34 + CD38 − cells in each condition (media and DJ001, n = 10; DJ001 + EHT1864, n = 5). One-way ANOVA with Tukey’s multiple comparison test. d Fold changes (2 −ΔΔCt ) in BCL2L1 and MCL-1 gene expression in CD34 + CD38 − cells at 12 h after 300 cGy in media ± DJ001 ( n = 3). Gene transcript changes were normalized to GAPDH and media treatment. Two-way ANOVA with Sidak’s multiple comparison test. e Schematic representation of NSG mice transplantation assay using the progeny of human BM CD34 + cells irradiated with 300 cGy and treated with or without DJ001 × 36 h. f Representative flow cytometric analysis of human CD45 + cells, human CD34 + cells, human CD19 + B cells, human CD33 + myeloid cells, and human CD3 + T cells engraftment in the BM of NSG mice at 12 weeks post transplantation. g Percent engraftment of human hematopoietic cell subsets in the BM of NSG mice at 12 weeks post transplantation ( n = 11–12/group). Source data are provided as Source Data File. * P

    Article Snippet: For MEP, CMP, GMP, and CLP cell analysis, BM cells were stained with APC-Cy7-conjugated anti-mouse Sca1 (BD Biosciences, #560654, 1:100), PE-conjugated anti-mouse ckit (BD Biosciences, #553355, 1:100), V450 lineage cocktail (BD Biosciences, #561301; 1:10), Alexa Fluor 488-conjugated anti-mouse CD127 (BD Biosciences, #561533, 1:100), Alexa Fluor 647-conjugated anti-mouse CD34 (BD Biosciences, #560230; 1:100), and BV605-conjugated anti-mouse CD16/32 antibodies (BD Biosciences, #563006; 1:100).

    Techniques: Irradiation, Staining, Expressing, Mouse Assay, Transplantation Assay

    Mesenchymal stem cell (MSC) characterization in normoxic and hypoxic conditions. a Positive MSC markers (CD105, CD73, and CD90) and b negative MSC markers (CD,14, CD34, CD45)

    Journal: Stem Cell Research & Therapy

    Article Title: Hypoxic preconditioning induces epigenetic changes and modifies swine mesenchymal stem cell angiogenesis and senescence in experimental atherosclerotic renal artery stenosis

    doi: 10.1186/s13287-021-02310-z

    Figure Lengend Snippet: Mesenchymal stem cell (MSC) characterization in normoxic and hypoxic conditions. a Positive MSC markers (CD105, CD73, and CD90) and b negative MSC markers (CD,14, CD34, CD45)

    Article Snippet: Conversely, MSC were expected to not express CD45 (Abcam, Cat. # ab51482), CD34 (BD Biosciences, San Jose, CA, Cat. # 340441), or CD14 (Abcam, Cat. # ab82012).

    Techniques:

    Phenotypic diversity of acute myeloid leukemia (AML) leukemic stem cells (LSCs). (A) CD34 and CD38 surface marker profiles of all 52 patients. (B) Gating strategy for AML-LSCs. (C) Three distinct populations were analyzed as LSC phenotypes: left, multipotent progenitor (MPP)-like LSC; middle, lymphoid primed multipotent progenitor (LMPP)-like LSC; right, granulocyte-macrophage progenitors (GMP)-like LSC.

    Journal: The Korean Journal of Internal Medicine

    Article Title: Leukemic stem cell phenotype is associated with mutational profile in acute myeloid leukemia

    doi: 10.3904/kjim.2020.014

    Figure Lengend Snippet: Phenotypic diversity of acute myeloid leukemia (AML) leukemic stem cells (LSCs). (A) CD34 and CD38 surface marker profiles of all 52 patients. (B) Gating strategy for AML-LSCs. (C) Three distinct populations were analyzed as LSC phenotypes: left, multipotent progenitor (MPP)-like LSC; middle, lymphoid primed multipotent progenitor (LMPP)-like LSC; right, granulocyte-macrophage progenitors (GMP)-like LSC.

    Article Snippet: Cells were stained with following anti-human monoclonal antibodies: CD45-APC/cy7 (557833), CD34-APC (555824), CD38-BV421 (562445), CD90-PE (555596), CD123-PE/Cy7 (560826), and CD45RA-PerCP/Cy5.5 (563429) (BD Bioscience).

    Techniques: Marker