apc anti human cd24  (BioLegend)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    APC anti human CD24
    Description:
    APC anti human CD24 ML5 Isotype Mouse IgG2a κ Reactivity Human Cross Reactivity Chimpanzee Baboon Apps FC Size 25 tests
    Catalog Number:
    311117
    Price:
    125
    Applications:
    FC
    Conjugate:
    APC
    Size:
    25 tests
    Category:
    Human Immunology
    Preparation:
    The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions The solution is free of unconjugated APC and unconjugated antibody
    Source:
    Mouse
    Quantity:
    1
    Buy from Supplier


    Structured Review

    BioLegend apc anti human cd24
    APC anti human CD24
    APC anti human CD24 ML5 Isotype Mouse IgG2a κ Reactivity Human Cross Reactivity Chimpanzee Baboon Apps FC Size 25 tests
    https://www.bioz.com/result/apc anti human cd24/product/BioLegend
    Average 95 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    apc anti human cd24 - by Bioz Stars, 2021-01
    95/100 stars

    Images

    Related Articles

    Flow Cytometry:

    Article Title: Gene Expression Profiling of the Response to Interferon Beta in Epstein-Barr-Transformed and Primary B Cells of Patients with Multiple Sclerosis
    Article Snippet: .. Flow cytometry LCLs were stained by multicolor flow cytometry with FITC anti-human CD19; APC-CY7 anti-human CD27; PerCP anti-human CD38; and APC anti-human CD24 (Biolegend). .. BD CompBeads (Becton and Dickinson) were used for compensation according to the manufacturer's instructions.

    Article Title: CCL20 triggered by chemotherapy hinders the therapeutic efficacy of breast cancer
    Article Snippet: .. In addition, single cells from tumor or cell line were also stained with CD24 antibody (Cat 311118, BioLegend, USA) and CD44 antibody (Cat 560532, BD Pharmingen, USA) for 30 minutes on ice and then resuspended with DAPI solution for flow cytometry analysis. .. A population of 10,000 living cells was captured and analyzed, and all fluorescence-activated cell sorting (FACS) experiments was performed using MOFLO ASTRIOS flow cytometer system (BECKMAN COULTER, USA) and software Summit 6.1.

    Fluorescence:

    Article Title: CRB3 downregulation confers breast cancer stem cell traits through TAZ/β-catenin
    Article Snippet: .. Fluorescence-activated cell sorting analysis One million cells were stained with anti-CD44-APC conjugate (103008; Biolegend, San Diego, CA, USA) and anti-CD24-PE conjugate (311118; Biolegend). .. The ALDEFLUOR kit (01700; Stem Cell Technologies, Vancouver, BC, Canada) was used to detect intracellular ALDH enzyme activity.

    Cytometry:

    Article Title: Gene Expression Profiling of the Response to Interferon Beta in Epstein-Barr-Transformed and Primary B Cells of Patients with Multiple Sclerosis
    Article Snippet: .. Flow cytometry LCLs were stained by multicolor flow cytometry with FITC anti-human CD19; APC-CY7 anti-human CD27; PerCP anti-human CD38; and APC anti-human CD24 (Biolegend). .. BD CompBeads (Becton and Dickinson) were used for compensation according to the manufacturer's instructions.

    Article Title: CCL20 triggered by chemotherapy hinders the therapeutic efficacy of breast cancer
    Article Snippet: .. In addition, single cells from tumor or cell line were also stained with CD24 antibody (Cat 311118, BioLegend, USA) and CD44 antibody (Cat 560532, BD Pharmingen, USA) for 30 minutes on ice and then resuspended with DAPI solution for flow cytometry analysis. .. A population of 10,000 living cells was captured and analyzed, and all fluorescence-activated cell sorting (FACS) experiments was performed using MOFLO ASTRIOS flow cytometer system (BECKMAN COULTER, USA) and software Summit 6.1.

    Incubation:

    Article Title: A CD44v+ subpopulation of breast cancer stem-like cells with enhanced lung metastasis capacity
    Article Snippet: .. For CD24-CD44-CD44v6 triple antibody analysis, one million cells were incubated with 5 μ l APC-anti-human CD24 (Biolegend 311118), 20 μ l PE-anti-human CD44 (BD Pharmingen 555479) and 5 μ l FITC-anti-human CD44v6 (R & D FAB3660F, Minneapolis, MN, USA) for 30 min at 4 °C. .. To detect CD44v7 expression, 500 thousands cells were incubated with 2 μ l rabbit anti-human CD44v7 (Millipore AB2083) for 30 min at 4 °C, followed by incubating with 1 μ l PE-goat anti-rabbit (Abcam ab97070) for 30 min at 4 °C.

    Article Title: LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients
    Article Snippet: .. To explore the proportion of reported stem cell markers in both LGR5+ and LGR5− glioma cells, the cells were incubated with APC anti-human CD133 antibody (#130–090-826; Miltenyi), APC anti-human CD44 antibody (#17–0441-82; eBioscience), APC anti-human CD24 antibody (#311118; BioLegend), APC anti-human CD90 antibody (#328114; BioLegend) or APC anti-human EpCAM (#328208; BioLegend) antibody for 30 min at room temperature. .. APC anti-human IgG (#409306; BioLegend) was used as an isotype control for these markers.

    Staining:

    Article Title: Gene Expression Profiling of the Response to Interferon Beta in Epstein-Barr-Transformed and Primary B Cells of Patients with Multiple Sclerosis
    Article Snippet: .. Flow cytometry LCLs were stained by multicolor flow cytometry with FITC anti-human CD19; APC-CY7 anti-human CD27; PerCP anti-human CD38; and APC anti-human CD24 (Biolegend). .. BD CompBeads (Becton and Dickinson) were used for compensation according to the manufacturer's instructions.

    Article Title: CCL20 triggered by chemotherapy hinders the therapeutic efficacy of breast cancer
    Article Snippet: .. In addition, single cells from tumor or cell line were also stained with CD24 antibody (Cat 311118, BioLegend, USA) and CD44 antibody (Cat 560532, BD Pharmingen, USA) for 30 minutes on ice and then resuspended with DAPI solution for flow cytometry analysis. .. A population of 10,000 living cells was captured and analyzed, and all fluorescence-activated cell sorting (FACS) experiments was performed using MOFLO ASTRIOS flow cytometer system (BECKMAN COULTER, USA) and software Summit 6.1.

    Article Title: CRB3 downregulation confers breast cancer stem cell traits through TAZ/β-catenin
    Article Snippet: .. Fluorescence-activated cell sorting analysis One million cells were stained with anti-CD44-APC conjugate (103008; Biolegend, San Diego, CA, USA) and anti-CD24-PE conjugate (311118; Biolegend). .. The ALDEFLUOR kit (01700; Stem Cell Technologies, Vancouver, BC, Canada) was used to detect intracellular ALDH enzyme activity.

    FACS:

    Article Title: CRB3 downregulation confers breast cancer stem cell traits through TAZ/β-catenin
    Article Snippet: .. Fluorescence-activated cell sorting analysis One million cells were stained with anti-CD44-APC conjugate (103008; Biolegend, San Diego, CA, USA) and anti-CD24-PE conjugate (311118; Biolegend). .. The ALDEFLUOR kit (01700; Stem Cell Technologies, Vancouver, BC, Canada) was used to detect intracellular ALDH enzyme activity.

    Article Title: PIM protein kinases regulate the level of the long noncoding RNA H19 to control stem cell gene transcription and modulate tumor growth
    Article Snippet: .. Antibodies used for our FACS analyses include APC anti‐human CD24 antibody (Cat # 311117; Biolegend, San Diego, CA, USA), PE anti‐human CD29 antibody (Cat # 303003; Biolegend), APC anti‐human CD133 (Cat # 17‐1338‐41; eBioscience, San Diego, CA, USA), and APC anti‐human CD49b (integrin alpha‐2; Cat # 17‐0500‐41; eBioscience). .. 2.13 Affymetrix gene chip expression analysis The microarray was performed as described previously (Padi et al. , ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    BioLegend mouse apc conjugated antihuman cd24
    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, <t>CD24</t> and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 1. A : Scatter plot shows the co-expression of EpCAM with CD44 B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM + cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. <t>CD24-APC</t> represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.
    Mouse Apc Conjugated Antihuman Cd24, supplied by BioLegend, used in various techniques. Bioz Stars score: 95/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse apc conjugated antihuman cd24/product/BioLegend
    Average 95 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    mouse apc conjugated antihuman cd24 - by Bioz Stars, 2021-01
    95/100 stars
      Buy from Supplier

    90
    BioLegend allophycocyanin apc conjugated mouse anti human cd24
    Two color cytofluorometric analysis on RCC52 cells and the clonal sublines Immunophenotypic profiles of the parental, epithelioid (E1~E4) and fibroblastoid (M1~M4) clonal sublines of the RCC52 cell line as determined by two color analysis with anti-CD44 and <t>anti-CD24</t> mAbs conjugated with FITC and <t>APC</t> respectively. A . The expression of CD44/CD24 profile of different passage of RCC52 cells. B . The expression of CD44/CD24 profile in epithelioid and fibroblastoid sublines.
    Allophycocyanin Apc Conjugated Mouse Anti Human Cd24, supplied by BioLegend, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allophycocyanin apc conjugated mouse anti human cd24/product/BioLegend
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    allophycocyanin apc conjugated mouse anti human cd24 - by Bioz Stars, 2021-01
    90/100 stars
      Buy from Supplier

    Image Search Results


    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 1. A : Scatter plot shows the co-expression of EpCAM with CD44 B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM + cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 1. A : Scatter plot shows the co-expression of EpCAM with CD44 B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM + cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Expressing, Binding Assay, Labeling

    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 6. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 6. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Expressing, Binding Assay, Labeling

    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 7. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 7. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Expressing, Binding Assay, Labeling

    Percentage positivity of putative cancer stem cell markers in primary retinoblastoma (RB) tumors. A : Representative scatter plot shows 61.24% of epithelial cell adhesion molecule (EpCAM) positive cells in the RB tumor. B : Representative scatter plot shows 63.67% of cluster determinant (CD)44 positive cells in the RB tumors. C : Representative scatter plot shows 63.67% of CD24 positive cells in the RB tumors. D : Representative scatter plot shows 1.5% of ATP binding cassette protein G2 (ABCG2) positive cells in the RB tumors. EpCAM-FITC represents EpCAM + cells identified using fluorescein isothiocyanate (FITC) labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-FITC represents ABCG2 positive cells identified using FITC labeled antibody.

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Percentage positivity of putative cancer stem cell markers in primary retinoblastoma (RB) tumors. A : Representative scatter plot shows 61.24% of epithelial cell adhesion molecule (EpCAM) positive cells in the RB tumor. B : Representative scatter plot shows 63.67% of cluster determinant (CD)44 positive cells in the RB tumors. C : Representative scatter plot shows 63.67% of CD24 positive cells in the RB tumors. D : Representative scatter plot shows 1.5% of ATP binding cassette protein G2 (ABCG2) positive cells in the RB tumors. EpCAM-FITC represents EpCAM + cells identified using fluorescein isothiocyanate (FITC) labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-FITC represents ABCG2 positive cells identified using FITC labeled antibody.

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Binding Assay, Labeling

    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 3. A : Scatter plot shows the co-expression of EpCAM with Cluster determinant-44 (CD44). B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 3. A : Scatter plot shows the co-expression of EpCAM with Cluster determinant-44 (CD44). B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Expressing, Binding Assay, Labeling

    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 2. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM + cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 2. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM + cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Expressing, Binding Assay, Labeling

    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 4. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody..

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 4. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody..

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Expressing, Binding Assay, Labeling

    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 8. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM + cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 8. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM + cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Expressing, Binding Assay, Labeling

    Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 5. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Journal: Molecular Vision

    Article Title: EpCAM is a putative stem marker in retinoblastoma and an effective target for T-cell-mediated immunotherapy

    doi:

    Figure Lengend Snippet: Investigation of the co-expression of epithelial cell adhesion molecule (EpCAM) with cluster determinant (CD)44, CD24 and ATP binding cassette protein G2 (ABCG2) markers in retinoblastoma (RB) tumor 5. A : Scatter plot shows the co-expression of EpCAM with CD44. B : Scatter plot shows the co-expression of EpCAM with CD24. C : Scatter plot shows the co-expression of EpCAM with ABCG2. EpCAM-fluorescein isothiocyanate (FITC) represents EpCAM positive cells identified using FITC labeled antibody. CD44-PE represents CD44 positive cells identified using phycoerythrin labeled antibody. CD24-APC represents CD24 positive cells identified using allophycocyanin labeled antibody. ABCG2-APC represents ABCG2 positive cells identified using allophycocyanin labeled antibody.

    Article Snippet: The antibodies used were mouse FITC-conjugated antihuman EpCAM (B29.1; 0.1 mg/ml; Abcam, Cambridge, UK), mouse PE-conjugated antihuman CD44 (F10, 0.1 mg/ml; Abcam), mouse APC-conjugated antihuman CD24 (ML5, 25 µg/ml; Biolegend, San Diego, CA), and mouse FITC-conjugated antihuman ABCG2 (CD3, 25 µg/ml; Biolegend).

    Techniques: Expressing, Binding Assay, Labeling

    Two color cytofluorometric analysis on RCC52 cells and the clonal sublines Immunophenotypic profiles of the parental, epithelioid (E1~E4) and fibroblastoid (M1~M4) clonal sublines of the RCC52 cell line as determined by two color analysis with anti-CD44 and anti-CD24 mAbs conjugated with FITC and APC respectively. A . The expression of CD44/CD24 profile of different passage of RCC52 cells. B . The expression of CD44/CD24 profile in epithelioid and fibroblastoid sublines.

    Journal: Oncotarget

    Article Title: Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells

    doi: 10.18632/oncotarget.14777

    Figure Lengend Snippet: Two color cytofluorometric analysis on RCC52 cells and the clonal sublines Immunophenotypic profiles of the parental, epithelioid (E1~E4) and fibroblastoid (M1~M4) clonal sublines of the RCC52 cell line as determined by two color analysis with anti-CD44 and anti-CD24 mAbs conjugated with FITC and APC respectively. A . The expression of CD44/CD24 profile of different passage of RCC52 cells. B . The expression of CD44/CD24 profile in epithelioid and fibroblastoid sublines.

    Article Snippet: For multiple-color phenotyping, fluorescein isothioyanate (FITC)-conjugated mouse anti-human CD44 (clone G44-26, BD Pharmingen, San Diego, CA) and allophycocyanin (APC)-conjugated mouse anti-human CD24 (clone ML5, Biolegend, San Diego, CA) mAbs were also used according to the manufacturer's instructions.

    Techniques: Expressing

    Two color cytofluorometric analysis on six different histologic RCC cell lines Six different histologic RCC cell lines, sarcomatoid RCC52, chromophobe RCC98, papillary HH050, tubular HH244, clear cell HH332 and HOKN-9, were tested with anti-CD44 and anti-CD24 mAbs conjugated with FITC and APC respectively, as test reagents.

    Journal: Oncotarget

    Article Title: Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells

    doi: 10.18632/oncotarget.14777

    Figure Lengend Snippet: Two color cytofluorometric analysis on six different histologic RCC cell lines Six different histologic RCC cell lines, sarcomatoid RCC52, chromophobe RCC98, papillary HH050, tubular HH244, clear cell HH332 and HOKN-9, were tested with anti-CD44 and anti-CD24 mAbs conjugated with FITC and APC respectively, as test reagents.

    Article Snippet: For multiple-color phenotyping, fluorescein isothioyanate (FITC)-conjugated mouse anti-human CD44 (clone G44-26, BD Pharmingen, San Diego, CA) and allophycocyanin (APC)-conjugated mouse anti-human CD24 (clone ML5, Biolegend, San Diego, CA) mAbs were also used according to the manufacturer's instructions.

    Techniques: