apali restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs apali restriction enzyme
    Confirmation of chromatin looping by 3C assay with <t>BglII</t> and/or <t>ApaLI</t> restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII
    Apali Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apali restriction enzyme/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    apali restriction enzyme - by Bioz Stars, 2020-05
    94/100 stars

    Images

    1) Product Images from "Sp1 Regulates Chromatin Looping between an Intronic Enhancer and Distal Promoter of the Human Heme Oxygenase-1 Gene in Renal Cells *"

    Article Title: Sp1 Regulates Chromatin Looping between an Intronic Enhancer and Distal Promoter of the Human Heme Oxygenase-1 Gene in Renal Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M109.058586

    Confirmation of chromatin looping by 3C assay with BglII and/or ApaLI restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII
    Figure Legend Snippet: Confirmation of chromatin looping by 3C assay with BglII and/or ApaLI restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII

    Techniques Used:

    Related Articles

    In Vivo:

    Article Title: Identification of Bacteriophage N4 Virion RNA Polymerase-Nucleic Acid Interactions in Transcription Complexes *
    Article Snippet: .. Reporter Plasmids to Determine in Vivo Activity of Mini-vRNAP —A fragment flanked by ApaLI- and BamHI restriction sites that includes vRNAP promoter P2 (–19 to +2) followed by the lacZ ′ gene from M13mp18 was inserted into ApaLI and BamHI-digested pACYC177 (New England Biolabs), disrupting the β-lactamase gene. .. The resulting plasmid, pIK1, was used to determine in vivo activity of N4 mini-vRNAP variants.

    Agarose Gel Electrophoresis:

    Article Title: Stimulation of homology-directed gene targeting at an endogenous human locus by a nicking endonuclease
    Article Snippet: .. After overnight digestion with ApaLI (New England Biolabs), 10 µg DNA samples were resolved in a 0.8% agarose gel in 1× Tris–acetate–EDTA buffer. .. Next, the DNA was transferred by capillary action to an Amersham Hybond-XL membrane (GE Healthcare) using a standard Southern blot technique.

    Mutagenesis:

    Article Title: Different allelic frequency of progressive rod-cone degeneration in two populations of Labrador Retrievers in Japan
    Article Snippet: .. The PCR product was digested with 5 units of ApaLI (New England Biolab, MA, U.S.A.) at 37°C for 90 min to yield 151 and 81-bp fragments in the wild genotype (GG), but not in the mutant genotype (AA). .. After digestion, the PCR products were electrophoresed on a 2% agarose gel and visualized by UltraPowerTM DNA Dye solution (Gelex International, Tokyo, Japan).

    Isolation:

    Article Title: RIP140 in thyroid hormone-repression and chromatin remodeling of Crabp1 gene during adipocyte differentiation
    Article Snippet: .. Isolated nuclei from differentiating 3T3-L1 cells were digested with 100 U of PstI, XhoI, SmaI, SpeI and ApaLI (New England Biolabs) for 30 min. .. The purified genomic DNA was re-digested with 100 U of ApaI completely (for first digestion with XhoI, PstI and SmaI) or 100 U of PstI (for first digestion with SpeI and ApaLI).

    Activity Assay:

    Article Title: Identification of Bacteriophage N4 Virion RNA Polymerase-Nucleic Acid Interactions in Transcription Complexes *
    Article Snippet: .. Reporter Plasmids to Determine in Vivo Activity of Mini-vRNAP —A fragment flanked by ApaLI- and BamHI restriction sites that includes vRNAP promoter P2 (–19 to +2) followed by the lacZ ′ gene from M13mp18 was inserted into ApaLI and BamHI-digested pACYC177 (New England Biolabs), disrupting the β-lactamase gene. .. The resulting plasmid, pIK1, was used to determine in vivo activity of N4 mini-vRNAP variants.

    Polymerase Chain Reaction:

    Article Title: Different allelic frequency of progressive rod-cone degeneration in two populations of Labrador Retrievers in Japan
    Article Snippet: .. The PCR product was digested with 5 units of ApaLI (New England Biolab, MA, U.S.A.) at 37°C for 90 min to yield 151 and 81-bp fragments in the wild genotype (GG), but not in the mutant genotype (AA). .. After digestion, the PCR products were electrophoresed on a 2% agarose gel and visualized by UltraPowerTM DNA Dye solution (Gelex International, Tokyo, Japan).

    Article Title: Shikonin, dually functions as a proteasome inhibitor and a necroptosis inducer in multiple myeloma cells
    Article Snippet: .. ER stress was assessed by detecting activated XBP-1 , which was analyzed by digestion of PCR products with ApaLI (New England Biolabs Inc., MA, USA), as previously described ( ). .. Because the ApaLI site in XBP-1 mRNA is spliced out upon activation, activated XBP-1 shows one large band after ApaLI digestion, while inactivated XBP-1 shows two ApaLI-digested bands.

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    New England Biolabs apali restriction enzyme
    Confirmation of chromatin looping by 3C assay with <t>BglII</t> and/or <t>ApaLI</t> restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII
    Apali Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apali restriction enzyme/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    apali restriction enzyme - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    92
    New England Biolabs apal1 restriction enzyme
    Confirmation of chromatin looping by 3C assay with <t>BglII</t> and/or <t>ApaLI</t> restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII
    Apal1 Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apal1 restriction enzyme/product/New England Biolabs
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    apal1 restriction enzyme - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Confirmation of chromatin looping by 3C assay with BglII and/or ApaLI restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII

    Journal: The Journal of Biological Chemistry

    Article Title: Sp1 Regulates Chromatin Looping between an Intronic Enhancer and Distal Promoter of the Human Heme Oxygenase-1 Gene in Renal Cells *

    doi: 10.1074/jbc.M109.058586

    Figure Lengend Snippet: Confirmation of chromatin looping by 3C assay with BglII and/or ApaLI restriction enzyme. A , schematic showing potential interaction between the fragments containing HS-2 region and the internal enhancer ( square ) and transcription factor(s) ( TF ). B , BglII

    Article Snippet: SDS was then sequestered from the samples by the addition of 1.8% Triton X-100 (Fisher), and the samples were incubated at 37 °C for 1 h. The samples were digested with BglII and/or ApaLI restriction enzyme (New England Biolabs) at 37 °C for 16 h. Restriction enzymes were inactivated by the addition of 1.6% SDS and further incubation at 65 °C for 20 min.

    Techniques: