3 8 diamino 5ethyl 6 phenylphenanthridinium bromide etbr 2 7 dichlorodihydroflurescein  (Millipore)

 
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    Millipore 3 8 diamino 5ethyl 6 phenylphenanthridinium bromide etbr 2 7 dichlorodihydroflurescein
    3 8 Diamino 5ethyl 6 Phenylphenanthridinium Bromide Etbr 2 7 Dichlorodihydroflurescein, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    3 8 diamino 5ethyl 6 phenylphenanthridinium bromide etbr 2 7 dichlorodihydroflurescein - by Bioz Stars, 2023-11
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    etbr  (Millipore)

     
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    Millipore etbr
    Etbr, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    intracellular etbr concentration  (Millipore)

     
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    Millipore intracellular etbr concentration
    Examples of model fitting to experimental data . The figures represent the fitting of the model to the experimental data for the time course <t>of</t> <t>intracellular</t> <t>EtBr</t> concentration in E. coli strains AG100, AG100A and AG100 TET , incubated in the presence of different EtBr concentrations. In each case, the solid line represents the model fit.
    Intracellular Etbr Concentration, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    intracellular etbr concentration - by Bioz Stars, 2023-11
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    1) Product Images from "Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli"

    Article Title: Fluorometric determination of ethidium bromide efflux kinetics in Escherichia coli

    Journal: Journal of Biological Engineering

    doi: 10.1186/1754-1611-3-18

    Examples of model fitting to experimental data . The figures represent the fitting of the model to the experimental data for the time course of intracellular EtBr concentration in E. coli strains AG100, AG100A and AG100 TET , incubated in the presence of different EtBr concentrations. In each case, the solid line represents the model fit.
    Figure Legend Snippet: Examples of model fitting to experimental data . The figures represent the fitting of the model to the experimental data for the time course of intracellular EtBr concentration in E. coli strains AG100, AG100A and AG100 TET , incubated in the presence of different EtBr concentrations. In each case, the solid line represents the model fit.

    Techniques Used: Concentration Assay, Incubation

    etbr  (Millipore)

     
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    Millipore etbr
    Etbr, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/etbr/product/Millipore
    Average 86 stars, based on 1 article reviews
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    etbr - by Bioz Stars, 2023-11
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    ethidium bromide etbr  (Millipore)

     
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    Millipore ethidium bromide etbr
    Ethidium Bromide Etbr, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethidium bromide etbr/product/Millipore
    Average 86 stars, based on 1 article reviews
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    ethidium bromide etbr - by Bioz Stars, 2023-11
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    etbr  (Millipore)

     
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    Millipore etbr
    Etbr, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/etbr/product/Millipore
    Average 86 stars, based on 1 article reviews
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    etbr - by Bioz Stars, 2023-11
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    etbr  (Millipore)

     
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    Millipore etbr
    (A) 1, 3, and 7-day <t>EtBr</t> treatment reduced <t>SH-SY5Y</t> <t>mtDNA</t> levels by approximately 50%, 75%, and 95%. ρ0 cells displayed >99.9% relative mtDNA depletion. (B) Representative western blots labeled with antibodies recognizing the mtDNA-encoded mtCO2, nuclear-encoded COX4I1, and nuclear-encoded NDUFB8 proteins. Actin is included as a loading control. (C) COX4I1 and NDUFB8 mRNA levels were unaffected by the acute EtBr treatment, although the NDUFB8 mRNA level was slightly reduced in ρ0 cells. (D) By MTT assay the acutely mtDNA-depleted cells appeared viable throughout. (E) The acutely mtDNA-depleted cells grossly showed at most subtle morphologic changes. (F) The ρ0 cell cycle rate was slightly reduced as compared to the parent SH-SY5Y line. **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.
    Etbr, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/etbr/product/Millipore
    Average 86 stars, based on 1 article reviews
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    etbr - by Bioz Stars, 2023-11
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    1) Product Images from "Mitochondrial DNA Manipulations Affect Tau Oligomerization"

    Article Title: Mitochondrial DNA Manipulations Affect Tau Oligomerization

    Journal: Journal of Alzheimer's disease : JAD

    doi: 10.3233/JAD-200286

    (A) 1, 3, and 7-day EtBr treatment reduced SH-SY5Y mtDNA levels by approximately 50%, 75%, and 95%. ρ0 cells displayed >99.9% relative mtDNA depletion. (B) Representative western blots labeled with antibodies recognizing the mtDNA-encoded mtCO2, nuclear-encoded COX4I1, and nuclear-encoded NDUFB8 proteins. Actin is included as a loading control. (C) COX4I1 and NDUFB8 mRNA levels were unaffected by the acute EtBr treatment, although the NDUFB8 mRNA level was slightly reduced in ρ0 cells. (D) By MTT assay the acutely mtDNA-depleted cells appeared viable throughout. (E) The acutely mtDNA-depleted cells grossly showed at most subtle morphologic changes. (F) The ρ0 cell cycle rate was slightly reduced as compared to the parent SH-SY5Y line. **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.
    Figure Legend Snippet: (A) 1, 3, and 7-day EtBr treatment reduced SH-SY5Y mtDNA levels by approximately 50%, 75%, and 95%. ρ0 cells displayed >99.9% relative mtDNA depletion. (B) Representative western blots labeled with antibodies recognizing the mtDNA-encoded mtCO2, nuclear-encoded COX4I1, and nuclear-encoded NDUFB8 proteins. Actin is included as a loading control. (C) COX4I1 and NDUFB8 mRNA levels were unaffected by the acute EtBr treatment, although the NDUFB8 mRNA level was slightly reduced in ρ0 cells. (D) By MTT assay the acutely mtDNA-depleted cells appeared viable throughout. (E) The acutely mtDNA-depleted cells grossly showed at most subtle morphologic changes. (F) The ρ0 cell cycle rate was slightly reduced as compared to the parent SH-SY5Y line. **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.

    Techniques Used: Western Blot, Labeling, MTT Assay

    (A) A progressive decline in complex I and COX activities accompanied the progressive EtBr-induced mtDNA depletion, although after 3 and 7 days of EtBr COX activity fell to a greater extent than complex I activity. CS activity did not decline during this period. (B) SH-SY5Y oxygen consumption rates also tracked mtDNA depletion, and as was the case with the ρ0 cells the mitochondrial OCR was essentially undetectable by EtBr exposure day 7. The EtBr-treated cell ECAR fell initially but recovered, and the EtBr exposure day 7 ECAR exceeded the ρ0 cell ECAR. n=16 per group. (C) Metabolic flux rates for the different groups; the EtBr day 7 cells and ρ0 cells both showed no or essentially no mitochondrial oxygen consumption, but the ρ0 cells uniquely lowered their glycolysis flux. *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.
    Figure Legend Snippet: (A) A progressive decline in complex I and COX activities accompanied the progressive EtBr-induced mtDNA depletion, although after 3 and 7 days of EtBr COX activity fell to a greater extent than complex I activity. CS activity did not decline during this period. (B) SH-SY5Y oxygen consumption rates also tracked mtDNA depletion, and as was the case with the ρ0 cells the mitochondrial OCR was essentially undetectable by EtBr exposure day 7. The EtBr-treated cell ECAR fell initially but recovered, and the EtBr exposure day 7 ECAR exceeded the ρ0 cell ECAR. n=16 per group. (C) Metabolic flux rates for the different groups; the EtBr day 7 cells and ρ0 cells both showed no or essentially no mitochondrial oxygen consumption, but the ρ0 cells uniquely lowered their glycolysis flux. *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.

    Techniques Used: Activity Assay

    (A) Tyrosine hydroxylase protein, a marker of differentiation, increased in staurosporine-treated SH-SY5Y cells. (B) The amount of mtDNA progressively declined in EtBr-treated differentiated cells. (C) Dot blots of differentiated SH-SY5Y cell lysates labeled with the TOC1 antibody, the Tau12 antibody, or Amido Black. Each day’s EtBR-treated samples were compared to control samples simultaneously prepared on that specific day. (D) Densitometry analysis reveals after 1 and 3 days of EtBr, TOC1 staining increases when normalized to total protein. (E) Densitometry analysis reveals after 1 and 3 days of EtBr, TOC1 staining increases when normalized to total tau protein, indicative of a monomer to oligomer shift. n=6 per group. *p<0.05, **p<0.01. Error bars represent SEM.
    Figure Legend Snippet: (A) Tyrosine hydroxylase protein, a marker of differentiation, increased in staurosporine-treated SH-SY5Y cells. (B) The amount of mtDNA progressively declined in EtBr-treated differentiated cells. (C) Dot blots of differentiated SH-SY5Y cell lysates labeled with the TOC1 antibody, the Tau12 antibody, or Amido Black. Each day’s EtBR-treated samples were compared to control samples simultaneously prepared on that specific day. (D) Densitometry analysis reveals after 1 and 3 days of EtBr, TOC1 staining increases when normalized to total protein. (E) Densitometry analysis reveals after 1 and 3 days of EtBr, TOC1 staining increases when normalized to total tau protein, indicative of a monomer to oligomer shift. n=6 per group. *p<0.05, **p<0.01. Error bars represent SEM.

    Techniques Used: Marker, Labeling, Staining

    etbr  (Millipore)

     
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    Millipore etbr
    (A) 1, 3, and 7-day <t>EtBr</t> treatment reduced <t>SH-SY5Y</t> <t>mtDNA</t> levels by approximately 50%, 75%, and 95%. ρ0 cells displayed >99.9% relative mtDNA depletion. (B) Representative western blots labeled with antibodies recognizing the mtDNA-encoded mtCO2, nuclear-encoded COX4I1, and nuclear-encoded NDUFB8 proteins. Actin is included as a loading control. (C) COX4I1 and NDUFB8 mRNA levels were unaffected by the acute EtBr treatment, although the NDUFB8 mRNA level was slightly reduced in ρ0 cells. (D) By MTT assay the acutely mtDNA-depleted cells appeared viable throughout. (E) The acutely mtDNA-depleted cells grossly showed at most subtle morphologic changes. (F) The ρ0 cell cycle rate was slightly reduced as compared to the parent SH-SY5Y line. **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.
    Etbr, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/etbr/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    etbr - by Bioz Stars, 2023-11
    86/100 stars

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    1) Product Images from "Mitochondrial DNA Manipulations Affect Tau Oligomerization"

    Article Title: Mitochondrial DNA Manipulations Affect Tau Oligomerization

    Journal: Journal of Alzheimer's disease : JAD

    doi: 10.3233/JAD-200286

    (A) 1, 3, and 7-day EtBr treatment reduced SH-SY5Y mtDNA levels by approximately 50%, 75%, and 95%. ρ0 cells displayed >99.9% relative mtDNA depletion. (B) Representative western blots labeled with antibodies recognizing the mtDNA-encoded mtCO2, nuclear-encoded COX4I1, and nuclear-encoded NDUFB8 proteins. Actin is included as a loading control. (C) COX4I1 and NDUFB8 mRNA levels were unaffected by the acute EtBr treatment, although the NDUFB8 mRNA level was slightly reduced in ρ0 cells. (D) By MTT assay the acutely mtDNA-depleted cells appeared viable throughout. (E) The acutely mtDNA-depleted cells grossly showed at most subtle morphologic changes. (F) The ρ0 cell cycle rate was slightly reduced as compared to the parent SH-SY5Y line. **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.
    Figure Legend Snippet: (A) 1, 3, and 7-day EtBr treatment reduced SH-SY5Y mtDNA levels by approximately 50%, 75%, and 95%. ρ0 cells displayed >99.9% relative mtDNA depletion. (B) Representative western blots labeled with antibodies recognizing the mtDNA-encoded mtCO2, nuclear-encoded COX4I1, and nuclear-encoded NDUFB8 proteins. Actin is included as a loading control. (C) COX4I1 and NDUFB8 mRNA levels were unaffected by the acute EtBr treatment, although the NDUFB8 mRNA level was slightly reduced in ρ0 cells. (D) By MTT assay the acutely mtDNA-depleted cells appeared viable throughout. (E) The acutely mtDNA-depleted cells grossly showed at most subtle morphologic changes. (F) The ρ0 cell cycle rate was slightly reduced as compared to the parent SH-SY5Y line. **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.

    Techniques Used: Western Blot, Labeling, MTT Assay

    (A) A progressive decline in complex I and COX activities accompanied the progressive EtBr-induced mtDNA depletion, although after 3 and 7 days of EtBr COX activity fell to a greater extent than complex I activity. CS activity did not decline during this period. (B) SH-SY5Y oxygen consumption rates also tracked mtDNA depletion, and as was the case with the ρ0 cells the mitochondrial OCR was essentially undetectable by EtBr exposure day 7. The EtBr-treated cell ECAR fell initially but recovered, and the EtBr exposure day 7 ECAR exceeded the ρ0 cell ECAR. n=16 per group. (C) Metabolic flux rates for the different groups; the EtBr day 7 cells and ρ0 cells both showed no or essentially no mitochondrial oxygen consumption, but the ρ0 cells uniquely lowered their glycolysis flux. *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.
    Figure Legend Snippet: (A) A progressive decline in complex I and COX activities accompanied the progressive EtBr-induced mtDNA depletion, although after 3 and 7 days of EtBr COX activity fell to a greater extent than complex I activity. CS activity did not decline during this period. (B) SH-SY5Y oxygen consumption rates also tracked mtDNA depletion, and as was the case with the ρ0 cells the mitochondrial OCR was essentially undetectable by EtBr exposure day 7. The EtBr-treated cell ECAR fell initially but recovered, and the EtBr exposure day 7 ECAR exceeded the ρ0 cell ECAR. n=16 per group. (C) Metabolic flux rates for the different groups; the EtBr day 7 cells and ρ0 cells both showed no or essentially no mitochondrial oxygen consumption, but the ρ0 cells uniquely lowered their glycolysis flux. *p<0.05, **p<0.01, ***p<0.001, and ****p<0.0001. Error bars represent SEM.

    Techniques Used: Activity Assay

    (A) Tyrosine hydroxylase protein, a marker of differentiation, increased in staurosporine-treated SH-SY5Y cells. (B) The amount of mtDNA progressively declined in EtBr-treated differentiated cells. (C) Dot blots of differentiated SH-SY5Y cell lysates labeled with the TOC1 antibody, the Tau12 antibody, or Amido Black. Each day’s EtBR-treated samples were compared to control samples simultaneously prepared on that specific day. (D) Densitometry analysis reveals after 1 and 3 days of EtBr, TOC1 staining increases when normalized to total protein. (E) Densitometry analysis reveals after 1 and 3 days of EtBr, TOC1 staining increases when normalized to total tau protein, indicative of a monomer to oligomer shift. n=6 per group. *p<0.05, **p<0.01. Error bars represent SEM.
    Figure Legend Snippet: (A) Tyrosine hydroxylase protein, a marker of differentiation, increased in staurosporine-treated SH-SY5Y cells. (B) The amount of mtDNA progressively declined in EtBr-treated differentiated cells. (C) Dot blots of differentiated SH-SY5Y cell lysates labeled with the TOC1 antibody, the Tau12 antibody, or Amido Black. Each day’s EtBR-treated samples were compared to control samples simultaneously prepared on that specific day. (D) Densitometry analysis reveals after 1 and 3 days of EtBr, TOC1 staining increases when normalized to total protein. (E) Densitometry analysis reveals after 1 and 3 days of EtBr, TOC1 staining increases when normalized to total tau protein, indicative of a monomer to oligomer shift. n=6 per group. *p<0.05, **p<0.01. Error bars represent SEM.

    Techniques Used: Marker, Labeling, Staining

    ethidium bromide etbr co ip  (Millipore)

     
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    Millipore ethidium bromide etbr co ip
    (A-B) , MM134 cells were hormone-deprived prior to siRNA transfection, then treated with vehicle (0.01% EtOH), 100pM E2, 100nM 4OHT, or 100nM Cl-4AS-1 (synthetic androgen). After 6d, proliferation was measured by dsDNA quantification. (A) , Growth vs mock siRNA control for E2 and 4OHT treatments. ‘Group’ represents context for gene inclusion in siRNA panel: RIME= identified by RIME; WNT4= transcription factor motif at ER binding site in WNT4 gene; OE= over-expressed in ER+ ILC vs ER+ IDC. (B) , Data from screen in (A). *, p<0.05; n.s. = not significant; ANOVA w/ Dunnett’s multiple correction. (C) , Nuclei (input) from cells in full serum were extracted prior to <t>co-IP;</t> ER co-IP enriched MDC1 vs IgG in ILC cell lines but not MCF7 (IDC). (D) , Nuclear extracts were treated with benzonase (+Bz) or <t>ethidium</t> bromide <t>(+EtBr)</t> prior to co-IP to disrupt DNA structure and prevent DNA-dependent co-IP. MDC1 co-IP enriched ER vs IgG, reciprocal co-IP to (C). (E) , Peptide counts from Martin et al 2017, points represent biological triplicate RIME samples. (F) , Breast cancer cells were transfected with siNT or siMDC1 48hrs prior to lysate harvest for immunoblot. -, mock transfection (reagent only). Single v double bands at ∼250kD are related to polyacrylamide gel strength. Ponceau stain for total protein shown as loading control. (G) , Proximity ligation assay (PLA) in MM134 for ER:MDC1 (left) or ER:FOXA1 (right, as control). (H) , PLA for ER:MDC1 as in (G). Values in G-H represent mean foci/cell +/- SD; complete quantification for (G-H) and statistical comparisons are shown in .
    Ethidium Bromide Etbr Co Ip, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethidium bromide etbr co ip/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethidium bromide etbr co ip - by Bioz Stars, 2023-11
    86/100 stars

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    1) Product Images from "Mediator of DNA damage checkpoint 1 (MDC1) is a novel estrogen receptor co-regulator in invasive lobular carcinoma of the breast"

    Article Title: Mediator of DNA damage checkpoint 1 (MDC1) is a novel estrogen receptor co-regulator in invasive lobular carcinoma of the breast

    Journal: bioRxiv

    doi: 10.1101/2020.12.16.423142

    (A-B) , MM134 cells were hormone-deprived prior to siRNA transfection, then treated with vehicle (0.01% EtOH), 100pM E2, 100nM 4OHT, or 100nM Cl-4AS-1 (synthetic androgen). After 6d, proliferation was measured by dsDNA quantification. (A) , Growth vs mock siRNA control for E2 and 4OHT treatments. ‘Group’ represents context for gene inclusion in siRNA panel: RIME= identified by RIME; WNT4= transcription factor motif at ER binding site in WNT4 gene; OE= over-expressed in ER+ ILC vs ER+ IDC. (B) , Data from screen in (A). *, p<0.05; n.s. = not significant; ANOVA w/ Dunnett’s multiple correction. (C) , Nuclei (input) from cells in full serum were extracted prior to co-IP; ER co-IP enriched MDC1 vs IgG in ILC cell lines but not MCF7 (IDC). (D) , Nuclear extracts were treated with benzonase (+Bz) or ethidium bromide (+EtBr) prior to co-IP to disrupt DNA structure and prevent DNA-dependent co-IP. MDC1 co-IP enriched ER vs IgG, reciprocal co-IP to (C). (E) , Peptide counts from Martin et al 2017, points represent biological triplicate RIME samples. (F) , Breast cancer cells were transfected with siNT or siMDC1 48hrs prior to lysate harvest for immunoblot. -, mock transfection (reagent only). Single v double bands at ∼250kD are related to polyacrylamide gel strength. Ponceau stain for total protein shown as loading control. (G) , Proximity ligation assay (PLA) in MM134 for ER:MDC1 (left) or ER:FOXA1 (right, as control). (H) , PLA for ER:MDC1 as in (G). Values in G-H represent mean foci/cell +/- SD; complete quantification for (G-H) and statistical comparisons are shown in .
    Figure Legend Snippet: (A-B) , MM134 cells were hormone-deprived prior to siRNA transfection, then treated with vehicle (0.01% EtOH), 100pM E2, 100nM 4OHT, or 100nM Cl-4AS-1 (synthetic androgen). After 6d, proliferation was measured by dsDNA quantification. (A) , Growth vs mock siRNA control for E2 and 4OHT treatments. ‘Group’ represents context for gene inclusion in siRNA panel: RIME= identified by RIME; WNT4= transcription factor motif at ER binding site in WNT4 gene; OE= over-expressed in ER+ ILC vs ER+ IDC. (B) , Data from screen in (A). *, p<0.05; n.s. = not significant; ANOVA w/ Dunnett’s multiple correction. (C) , Nuclei (input) from cells in full serum were extracted prior to co-IP; ER co-IP enriched MDC1 vs IgG in ILC cell lines but not MCF7 (IDC). (D) , Nuclear extracts were treated with benzonase (+Bz) or ethidium bromide (+EtBr) prior to co-IP to disrupt DNA structure and prevent DNA-dependent co-IP. MDC1 co-IP enriched ER vs IgG, reciprocal co-IP to (C). (E) , Peptide counts from Martin et al 2017, points represent biological triplicate RIME samples. (F) , Breast cancer cells were transfected with siNT or siMDC1 48hrs prior to lysate harvest for immunoblot. -, mock transfection (reagent only). Single v double bands at ∼250kD are related to polyacrylamide gel strength. Ponceau stain for total protein shown as loading control. (G) , Proximity ligation assay (PLA) in MM134 for ER:MDC1 (left) or ER:FOXA1 (right, as control). (H) , PLA for ER:MDC1 as in (G). Values in G-H represent mean foci/cell +/- SD; complete quantification for (G-H) and statistical comparisons are shown in .

    Techniques Used: Transfection, Binding Assay, Co-Immunoprecipitation Assay, Western Blot, Staining, Proximity Ligation Assay

    etbr  (Millipore)

     
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    Millipore etbr
    Etbr, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/etbr/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    etbr - by Bioz Stars, 2023-11
    86/100 stars

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