Journal: Frontiers in Neurology

Article Title: Ferroptosis-associated candidate genes in aneurysmal subarachnoid hemorrhage: evidence from human peripheral blood and mouse brain

doi: 10.3389/fneur.2026.1823039

Figure Lengend Snippet: Identification and functional enrichment of ferroptosis-related differentially expressed genes in the GSE36791 peripheral-blood dataset. (A) Heatmap of all differentially expressed genes in control and aSAH samples. (B) Heatmap of the 35 ferroptosis-related differentially expressed genes. (C) Venn diagrams showing the overlap between all genes detected in GSE36791 and human ferroptosis-related genes obtained from FerrDb, and the overlap between GSE36791 differentially expressed genes and FerrDb genes, identifying 35 ferroptosis-related differentially expressed genes. (D) Spearman correlation heatmap of the 35 ferroptosis-related differentially expressed genes. (E) Gene Ontology enrichment analysis of these genes. (F) Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of these genes. DEGs, differentially expressed genes; Ferr-DEGs, ferroptosis-related differentially expressed genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: Sections were then incubated overnight at 4 °C with the following primary antibodies: anti-PGD (1:100, Cat. A0563, Abclonal, Wuhan, China), anti-STAT3 (1:100, Cat. A11216, Abclonal), anti-MAPK3/ERK1 (1:100, Cat. A19561, Abclonal), and anti-G6PD (1:100, Cat. A27296, Abclonal).

Techniques: Functional Assay, Control, Analysis

Journal: Frontiers in Neurology

Article Title: Ferroptosis-associated candidate genes in aneurysmal subarachnoid hemorrhage: evidence from human peripheral blood and mouse brain

doi: 10.3389/fneur.2026.1823039

Figure Lengend Snippet: Network-based identification of ferroptosis-associated candidate hub genes in aSAH. (A) PPI network constructed from ferroptosis-related DEGs. (B) Top-ranked PPI hub genes identified by cytoHubba. (C–E) MCODE clusters identified from the PPI network, with scores of 4.286, 4.000, and 3.000, respectively. (F) Intersection between WGCNA module genes and PPI-derived hub genes. (G) Overlap between the four selected hub genes and DCI-associated genes. PPI, protein–protein interaction; DCI, delayed cerebral ischemia.

Article Snippet: Sections were then incubated overnight at 4 °C with the following primary antibodies: anti-PGD (1:100, Cat. A0563, Abclonal, Wuhan, China), anti-STAT3 (1:100, Cat. A11216, Abclonal), anti-MAPK3/ERK1 (1:100, Cat. A19561, Abclonal), and anti-G6PD (1:100, Cat. A27296, Abclonal).

Techniques: Construct, Derivative Assay

Journal: Chemistry & Biodiversity

Article Title: Network Pharmacology and In Vivo Evaluation of Modified Huanglian Wendan Decoction in Polycystic Ovary Syndrome With Insulin Resistance: Association With AGE–RAGE‐Related Signaling

doi: 10.1002/cbdv.71737

Figure Lengend Snippet: Ovarian AGE–RAGE/STAT3/AKT1‐related protein expression and phosphorylation. (A) Representative Western blot bands for AGE, RAGE, STAT3, AKT1, phospho‐STAT3 (Ser727), phospho‐AKT (Ser473), and GAPDH in the Control, Model, HLWDD‐L, HLWDD‐M, HLWDD‐H, and Metformin groups. (B) Relative densitometric analysis of AGE/GAPDH, RAGE/GAPDH, STAT3/GAPDH, AKT1/GAPDH, p‐STAT3/STAT3, and p‐AKT/AKT1. ** p < 0.01 vs. Control; ## p < 0.01 vs. Model. Densitometric comparisons were based on independent biological replicates and analyzed as described in Methods.

Article Snippet: Primary antibodies included AGE (A16422, ABclonal; 1:2000), RAGE (66833‐1‐Ig, Proteintech; 1:2000), STAT3 (60199‐1‐Ig, Proteintech; 1:1000), AKT1 (10176‐2‐AP, Proteintech; 1:2000), phospho‐STAT3 (Ser727; AP0715, ABclonal; 1:2000), phospho‐AKT (Ser473; 80455‐1‐RR, Proteintech; 1:1000), and GAPDH (60004‐1‐Ig, Proteintech; 1:20000).

Techniques: Expressing, Phospho-proteomics, Western Blot, Control, Analysis