antirat igg  (Abcam)

 
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  • 98
    Name:
    Rabbit Anti Rat IgG H L HRP
    Description:

    Catalog Number:
    ab102172
    Price:
    None
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    Structured Review

    Abcam antirat igg

    https://www.bioz.com/result/antirat igg/product/Abcam
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antirat igg - by Bioz Stars, 2020-09
    98/100 stars

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    Related Articles

    Western Blot:

    Article Title: Pathogenic ARH3 mutations result in ADP-ribose chromatin scars during DNA strand break repair
    Article Snippet: .. Secondary antibodies employed for western blotting were HRP-conjugated goat anti-rabbit (Bio-Rad; 170-6515), goat anti-mouse (Bio-Rad; 170-6516), and rabbit anti-rat (Abcam; ab6734) and for indirect immunofluorescence were goat anti-mouse or goat anti-rabbit Alexa 488 (Invitrogen; A-11001 and A-11008) and donkey anti-mouse or goat anti-rabbit Alexa 568 (Invitrogen; A-10037 and A-11011). .. PARP inhibitor (PARPi; Axon; KU 0058948), Olaparib (Cayman Chemical; AZD 2281), Veliparib (Selleckchem; ABT-888), and PARG inhibitor (PARGi; Sigma; PDD00017273) were dissolved in dimethyl sulfoxide (DMSO) (Sigma–Aldrich) to a working concentration of 10 mM.

    Incubation:

    Article Title: Effects of imipenem combined with low-dose cyclophosphamide on the intestinal barrier in septic rats
    Article Snippet: .. Membranes were subsequently incubated with primary antibodies occludin (1:5,000; cat. no. ab167161), claudin-2 (1:500; cat. no. ab53032; both Abcam, Cambridge, UK), ZO-1 (1:500; cat. no. sc-33725; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and β-actin (1:100; cat. no. ab8226) at 4°C overnight, followed by horseradish peroxidase-linked anti-rabbit IgG (1:10,000; cat. no. ab6734; both Abcam) secondary antibody for 2 h at room temperature. .. The films were then stored in a dark room and a chemiluminescent peroxidase substrate (cat. no. 34094; Thermo Fisher Scientific, Inc.) was applied according to the manufacturer's instructions.

    Article Title: Overexpression of a panel of cancer stem cell markers enhances the predictive capability of the progression and recurrence in the early stage cholangiocarcinoma
    Article Snippet: .. Plates were coated with 100 µl of serum and incubated at 4 °C for 18 h. Then, the plates were blocked with 200 µl of 5% skim milk and incubated at 37 °C for 1 h. After washing, 100 µl of primary antibodies (CD44; #ab2212; dilution 1:200) (Abcam, UK), (CD44v6; AB2080; dilution 1:800) (Merck, Germany), (CD44v8-10; #LKG-M001; dilution 1:10,000), and (EpCAM; #MA1-46104; dilution 1:200) (Thermo Fisher Scientific, USA) were added and incubated at 37 °C for 2 h. Then, the excess antibody was washed off and 100 µl of HRP-conjugated secondary antibodies were added (anti-mouse antibody for CD44; #A16166; dilution 1:400; (Thermo Fisher Scientific, USA), anti-rabbit antibody for CD44v6; #G21234; dilution 1:1000; (Invitrogen, USA), anti-rat antibody for CD44v8-10, #ab6734; dilution 1:25,000 (Abcam, UK), anti-mouse antibody for EpCAM, #A16166, dilution 1:400; (Thermo Fisher Scientific, USA)). .. After incubation, the excess antibody was washed off and the signal was developed using 100 µl of 1 mg/ml OPD substrate for 30 min.

    Article Title: MicroRNA-93 inhibits chondrocyte apoptosis and inflammation in osteoarthritis by targeting the TLR4/NF-κB signaling pathway
    Article Snippet: .. Detection was performed by incubation with peroxidase-conjugated secondary antibodies (cat. no. ab6734; 1:2,000; Abcam, Cambridge, UK) and chemiluminescence (EMD Millipore) for 1 h at room temperature. .. Densitometric analysis was performed using Quantity One software (v4.62; Bio-Rad Laboratories, Inc., Hercules, CA, USA). β-actin protein was used as the inner control of the cytoplasmic proteins; Histone H3 protein was used as the inner control of the nuclear proteins.

    other:

    Article Title: Smooth Muscle Cells of Penis in the Rat: Noninvasive Quantification with Shear Wave Elastography
    Article Snippet: Reagents The anti-alpha actin antibody [SA-20] (ab 82247) and rabbit anti-rat IgG H & L (HRP) (ab 6734) were obtained from Abcam (Shanghai, China).

    Immunofluorescence:

    Article Title: Pathogenic ARH3 mutations result in ADP-ribose chromatin scars during DNA strand break repair
    Article Snippet: .. Secondary antibodies employed for western blotting were HRP-conjugated goat anti-rabbit (Bio-Rad; 170-6515), goat anti-mouse (Bio-Rad; 170-6516), and rabbit anti-rat (Abcam; ab6734) and for indirect immunofluorescence were goat anti-mouse or goat anti-rabbit Alexa 488 (Invitrogen; A-11001 and A-11008) and donkey anti-mouse or goat anti-rabbit Alexa 568 (Invitrogen; A-10037 and A-11011). .. PARP inhibitor (PARPi; Axon; KU 0058948), Olaparib (Cayman Chemical; AZD 2281), Veliparib (Selleckchem; ABT-888), and PARG inhibitor (PARGi; Sigma; PDD00017273) were dissolved in dimethyl sulfoxide (DMSO) (Sigma–Aldrich) to a working concentration of 10 mM.

    Immunostaining:

    Article Title: Pituitary adenylate cyclase-activating polypeptide prevents contrast-induced nephropathy in a novel mouse model
    Article Snippet: .. We detected renal neutrophil infiltration by immunostaining kidney sections with a monoclonal rat anti-mouse neutrophil primary IgG antibody (mAb 7/4; Abcam, Cambridge, MA), a horseradish peroxidase-conjugated rabbit anti-rat IgG (ab6734; Abcam), and a 3,3′-diaminobenzidine substrate kit (Abcam) as described previously (Li et al. ). ..

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