antirabbit igg  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Name:
    Rabbit IgG Control Antibody
    Description:
    Rabbit IgG Control Antibody This IgG preparation is intended for use as a control for primary antibodies made in rabbit Supplied as a lyophilized powder this antibody has been purified from pooled serum of healthy adult animals and contains a spectrum of the IgG subclasses present in serum The control antibody should be applied to the tissue section at the same concentration as the primary antibody to indicate whether staining is specific for the antigen or is nonspecific adsorption of primary antibody to tissue sites
    Catalog Number:
    I-1000
    Price:
    None
    Host:
    Rabbit
    Size:
    5 mg
    Category:
    Antibodies
    Buy from Supplier


    Structured Review

    Vector Laboratories antirabbit igg
    Rabbit IgG Control Antibody
    Rabbit IgG Control Antibody This IgG preparation is intended for use as a control for primary antibodies made in rabbit Supplied as a lyophilized powder this antibody has been purified from pooled serum of healthy adult animals and contains a spectrum of the IgG subclasses present in serum The control antibody should be applied to the tissue section at the same concentration as the primary antibody to indicate whether staining is specific for the antigen or is nonspecific adsorption of primary antibody to tissue sites
    https://www.bioz.com/result/antirabbit igg/product/Vector Laboratories
    Average 99 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    antirabbit igg - by Bioz Stars, 2020-08
    99/100 stars

    Images

    Related Articles

    Negative Control:

    Article Title: Temporal Changes in Glutaredoxin 1 and Protein S-Glutathionylation in Allergic Airway Inflammation
    Article Snippet: .. As a negative control, normal rabbit IgG (Vector Laboratories) was used instead of Glrx1 rabbit polyclonal antibody. .. After washing with PBS, tissue sections were incubated with secondary antibody (Vector Laboratories) for 1 h. 3,3ʹ-Diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate.

    ChIP-chip:

    Article Title: A novel mechanism for the transcriptional regulation of Wnt signaling in development
    Article Snippet: .. ChIP and ChIP–chip assays were performed as described previously ( ; ), using pooled E13.5 C57Bl6 and Vax2−/− eyes as the starting material and α-Vax2 antibody ( ) or rabbit IgG (Vector Laboratories). .. The ChIP–chip hybridization was performed using NimbleGen (2.1M Mouse Deluxe Promoter array, Roche), and data were analyzed with CisGenome software ( ).

    Blocking Assay:

    Article Title: The Myc Target Gene JPO1/CDCA7 is Frequently Over-expressed in Human Tumors and has Limited Transforming Activity In Vivo
    Article Snippet: .. Following two more PBS washes, slides were quenched in 0.6% hydrogen peroxide/methanol, washed again in PBS and then blocked overnight in a humidifying chamber at 4° C using normal blocking serum. (Vector Laboratories, VectaStain Elite ABC kit) After washing in PBS three times, slides were incubated with either primary antibodies to JPO1 ( ) or rabbit IgG (Vector Labs) for at least one hour. .. Following another three PBS washes, slides were incubated with 2° antibody for 30 min – 1 hr and then detected with the VectaStain Elite ABC reagents and counterstained with haemotoxylin (Vector Laboratories).

    Concentration Assay:

    Article Title: Differential binding of chemokines to macrophages and neutrophils in the human inflamed synovium
    Article Snippet: .. Sections were rinsed in PBS, incubated with CXCR1 or CXCR2 antibodies (at 1/50 and 1/500 dilution respectively) or rabbit IgG (at the same concentration as primary antibodies) for 30 min. After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min. ..

    Article Title: Localisation of GPR30, a novel G protein-coupled oestrogen receptor, suggests multiple functions in rodent brain and peripheral tissues
    Article Snippet: .. The specificity of GPR30 antiserum has previously been confirmed , and normal rabbit IgG serum (Vector Laboratories, Peterborough, UK) was used as a control at the same concentration as the antibody. .. After washing (3×10 min with 0·1 M PBS), sections were incubated at room temperature in secondary biotinylated affinity purified goat anti-rabbit (1:500, Vector Laboratories), in 1% NGS/0·3% T-X in 0·1 M PBS for 1 h, washed (3×10 min with 0·1 M PBS) and incubated in horseradish peroxidase streptavadin (1:500, Vector Laboratories) in 1% NGS/0·3% T-X in 0·1 M PBS for a further hour.

    Incubation:

    Article Title: Transient Disruption of Intercellular Junctions Enables Baculovirus Entry into Nondividing Hepatocytes
    Article Snippet: .. Cultures were incubated in primary anti-Ac M NPV antibody or control rabbit immunoglobulin G (IgG) antibody (Vector Laboratories, Inc., Burlingame, Calif.) for 30 min, followed by three washes in PBS. ..

    Article Title: Differential binding of chemokines to macrophages and neutrophils in the human inflamed synovium
    Article Snippet: .. Sections were rinsed in PBS, incubated with CXCR1 or CXCR2 antibodies (at 1/50 and 1/500 dilution respectively) or rabbit IgG (at the same concentration as primary antibodies) for 30 min. After washing with PBS, the antibodies were detected using reagents in the Vectastain ABC Elite Kit (Vector Labs, Burlingame, USA); the sections were treated with biotinylated goat anti-rabbit IgG (at 1/200 dilution) for 30 min, rinsed with PBS, incubated with streptavidin-conjugated peroxidase for 30 min, washed with PBS, then treated with the chromogen 3,3'-diaminobenzidine for 5 min. ..

    Article Title: The Myc Target Gene JPO1/CDCA7 is Frequently Over-expressed in Human Tumors and has Limited Transforming Activity In Vivo
    Article Snippet: .. Following two more PBS washes, slides were quenched in 0.6% hydrogen peroxide/methanol, washed again in PBS and then blocked overnight in a humidifying chamber at 4° C using normal blocking serum. (Vector Laboratories, VectaStain Elite ABC kit) After washing in PBS three times, slides were incubated with either primary antibodies to JPO1 ( ) or rabbit IgG (Vector Labs) for at least one hour. .. Following another three PBS washes, slides were incubated with 2° antibody for 30 min – 1 hr and then detected with the VectaStain Elite ABC reagents and counterstained with haemotoxylin (Vector Laboratories).

    Article Title: Proteomic Analysis of Leptospira interrogans Shed in Urine of Chronically Infected Hosts ▿
    Article Snippet: .. Sections were first blocked with normal goat serum for 20 min and then incubated with anti-OMV (1:400) diluted in PBS for 30 min. Anti-OMV was detected with anti-rabbit immunoglobulin G (IgG) (1:200; Vector Laboratories). .. Finally, sections were washed three times with PBS for 5 min, incubated with Vectastain Elite ABC reagent for 30 min, and again washed three times with PBS for 5 min. Staining was visualized with a Vector NovaRED substrate for 5 min and the reaction stopped by rinsing sections with H2 O.

    Chromatin Immunoprecipitation:

    Article Title: A novel mechanism for the transcriptional regulation of Wnt signaling in development
    Article Snippet: .. ChIP and ChIP–chip assays were performed as described previously ( ; ), using pooled E13.5 C57Bl6 and Vax2−/− eyes as the starting material and α-Vax2 antibody ( ) or rabbit IgG (Vector Laboratories). .. The ChIP–chip hybridization was performed using NimbleGen (2.1M Mouse Deluxe Promoter array, Roche), and data were analyzed with CisGenome software ( ).

    Article Title: Genome-wide co-localization of active EGFR and downstream ERK pathway kinases mirrors mitogen-inducible RNA polymerase 2 genomic occupancy
    Article Snippet: .. Antibodies used in ChIP studies were as follows: non-specific rabbit IgG (I-1000, Vector Labs), Pol2 (4H8) (Santa cruz; sc-47701), pEGFR (Tyr845) (Cell signaling; 6963), pMek1/2 (Ser217/221) (Cell signaling; 9121), pErk1/2 (Thr202/Tyr204) (Cell signaling; 4370). .. ChIP-seq ChIPed DNA was subjected to a second round of shearing ( ) on Covaris S220 AFA ultrasonicator (Covaris Inc.) using the following parameters: Peak Incident Power (W): 175, Duty Factor: 10%, Cycles per Burst: 200, Treatment Time (s): 120.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Vector Laboratories texas red conjugated goat antirabbit igg
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or <t>antirabbit</t> <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Texas Red Conjugated Goat Antirabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/texas red conjugated goat antirabbit igg/product/Vector Laboratories
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    texas red conjugated goat antirabbit igg - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    99
    Vector Laboratories biotinylated antirabbit antibody
    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or <t>antirabbit</t> <t>IgG</t> conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.
    Biotinylated Antirabbit Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated antirabbit antibody/product/Vector Laboratories
    Average 99 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    biotinylated antirabbit antibody - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Murine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix In Vitro 1

    doi:

    Figure Lengend Snippet: TECs exhibit different cellular distributions of endothelial molecules than normal MHECs. TECs or MHECs were plated at subculture 2 onto glass coverslips precoated with either OnFN or hFN, respectively. At 2 days postconfluence, the cells were washed with DPBS + and fixed in ice-cold methanol for 5 min at -20°C. Cell surface adhesion molecules (CD31, β-catenin, ICAM-2, and VCAM-1) were stained with specific antibodies as described in Materials and Methods section and detected with antirat or antirabbit IgG conjugated to Texas Red or FITC. Fluorescence images were captured on an Axiovert inverted microscope equipped for fluorescence using a cooled CCD camera and IP Laboratories software. All images displayed were captured with a x 40 objective. These images are representative of three separate cultures of both MHECs and TECs generated from different source tissues at different times. Images of MHECs are shown in the left hand panels (a,c,e,g) and of TECs in the right hand panels (b,d,f,g) with staining for CD31 (a,b), β-catenin (c,d), ICAM-2 (e,f), and VCAM-1 (g,h). Junctional staining of CD31 and β-catenin was discontinuous in TECs, indicating the presence of fewer cell-cell junctions. In addition, VCAM-1 did not localize to cell-cell junctions in TECs. In contrast, normal MHECs showed continuous junctional staining for CD31, β-catenin, and VCAM-1. There was little difference in distribution of ICAM-2 between MHECs and TECs.

    Article Snippet: Texas Red-conjugated goat-antirat IgG, FITC-conjugated goat-antirabbit IgG (H + L), and Texas Red-conjugated goat-antirabbit IgG (H + L) were purchased from Vector Laboratories (Burlingame, CA).

    Techniques: Staining, Fluorescence, Inverted Microscopy, Software, Generated