antirabbit igg  (Jackson Immuno)

 
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  • 97
    Name:
    Goat Anti Rabbit IgG
    Description:
    Polyclonal antisera from immunized hosts are lipid extracted to improve clarity salt fractionated dialyzed against phosphate buffered saline containing sodium azide and freeze dried Antisera against whole serums are obtained by immunizing host animals with whole serum Antisera against whole IgG molecules i e Anti IgG H L are recommended for bridging PAP to primary antibodies Based on immunoelectrophoresis the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    Catalog Number:
    111-001-003
    Price:
    36
    Purity:
    The antibody was delipidated and ammonium sulfate fractionated from whole antiserum.
    Conjugate:
    Unconjugated
    Size:
    ml
    Category:
    Secondary Antibody
    Source:
    Goat
    Quantity:
    2 0
    Buy from Supplier


    Structured Review

    Jackson Immuno antirabbit igg
    Goat Anti Rabbit IgG
    Polyclonal antisera from immunized hosts are lipid extracted to improve clarity salt fractionated dialyzed against phosphate buffered saline containing sodium azide and freeze dried Antisera against whole serums are obtained by immunizing host animals with whole serum Antisera against whole IgG molecules i e Anti IgG H L are recommended for bridging PAP to primary antibodies Based on immunoelectrophoresis the antibody reacts with whole molecule rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against non immunoglobulin serum proteins The antibody may cross react with immunoglobulins from other species
    https://www.bioz.com/result/antirabbit igg/product/Jackson Immuno
    Average 97 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    antirabbit igg - by Bioz Stars, 2020-08
    97/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Early life voiding dysfunction leads to lower urinary tract dysfunction through alteration of muscarinic and purinergic signaling in the bladder
    Article Snippet: .. The filters were incubated with primary antibodies against P2X1 (1:500, cat. no. APR-022; Alomone Laboratories, Jerusalem, Israel), cholinergic receptor muscarinic 2 ( Chrm2 ) (1:500, NBP2–26152, Novus Biologicals, Littleton, CO), or glyceraldehyde-3-phosphate dehydrogenase ( Gapdh ) (1:1,000, cat. no. sc-25778, Santa Cruz Biotechnology, Dallas, TX) diluted in Tris-buffered saline-Tween 20, followed by treatment with horseradish peroxidase-conjugated anti-rabbit IgG (Novus Biologicals) or anti-goat IgG (Jackson ImmunoResearch, West Grove, PA). .. Signals were detected by development using Pierce ECL Western Blotting Substrate (Thermo Fisher Scientific).

    Article Title: The programmed DNA elimination and formation of micronuclei in germ line cells of the natural hybridogenetic water frog Pelophylax esculentus
    Article Snippet: .. Tissues were washed in PBS, and incubated in corresponding secondary antibodies (Alexa-488-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch Laboratories), Alexa-488-conjugated goat anti-mouse IgG (Molecular Probes) for 8 h at RT. .. Tissues were washed in PBS, and incubated in PBS containing 1 mg/ml DAPI overnight at RT.

    other:

    Article Title: Glomerular deposition of C1q and anti-C1q antibodies in mice following injection of antimouse C1q antibodies
    Article Snippet: Sera were diluted 1 : 1000 in PBS-T-BSA and rabbit IgG was detected using goat antirabbit IgG conjugated to HRP (Jackson).

    Binding Assay:

    Article Title: Glomerular deposition of C1q and anti-C1q antibodies in mice following injection of antimouse C1q antibodies
    Article Snippet: .. The binding of rabbit IgG was demonstrated using goat antirabbit IgG conjugated to HRP (Jackson Immuno Research Laboratory Inc.,West Grove, PA, USA). ..

    Article Title: Glomerular deposition of C1q and anti-C1q antibodies in mice following injection of antimouse C1q antibodies
    Article Snippet: .. After washing, rabbit IgG binding was detected using goat antirabbit IgG conjugated to HRP (Jackson) in PBS-T-BSA containing 1 m NaCl. .. Female BALB/C mice (Harlan), 6 weeks of age, had free access to water and standard chow.

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  • 85
    Jackson Immuno cy2 coupled goat antirabbit igg
    Immunolabeling of opsins in Moorfields/Institute of Ophthalmology-Müller 1 (MIO-M1) cells. A : Labeling of blue opsin and melanopsin, respectively, in MIO-M1 cells. The cells were counterstained against glutamine synthetase (GS) and GFAP, respectively. Double labeling yielded a yellow-orange merge signal. B : Labeling of rhodopsin and red-green opsin, respectively, in MIO-M1 cells. The cells were counterstained against GFAP. C : Control culture of MIO-M1 cells that was stained with secondary antibodies (goat <t>antirabbit</t> <t>IgG</t> and goat antimouse IgG) and without primary antibodies. No nonspecific labeling was observed either, following incubation with goat antirat IgG (not shown). D: Immunostaining of HEK-293 cells against melanopsin, blue opsin, and red green opsin, respectively. The cells were counterstained against glutamine synthetase (GS). Cell nuclei were labeled with the DNA dye Hoechst 33258 (blue). Bars are 20 µm.
    Cy2 Coupled Goat Antirabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy2 coupled goat antirabbit igg/product/Jackson Immuno
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy2 coupled goat antirabbit igg - by Bioz Stars, 2020-08
    85/100 stars
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    93
    Jackson Immuno alexa fluor 588 conjugated donkey antirabbit igg
    Nicotine inhibits cystic fibrosis transmembrane conductance regulator (CFTR) protein in A549 cells characterized by an immunofluorescent assay. A549 cells were transfected with pacAd5.huCFTR-ECL4HA plasmid for 12 hours and cultured in medium with or without 200 nm/L of nicotine for additional 24 hours. The cells were then used for assessing CFTR protein and hemagglutinin (HA) with mouse anti-CFTR antibody (1:100) and rabbit polyclonal antibody to HA (1:100), respectively. <t>Alexa</t> Fluor 488-labeled donkey-anti-mouse immunoglobulin G <t>(IgG)</t> secondary antibody (green) and Alexa Fluor 588-conjugated donkey-anti-rabbit IgG antibody (red) were used as secondary antibodies for visualizing respective proteins of interest. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. A-F, Representative images showed that nicotine was able to suppress the abundance of both membrane and cytoplasmic CFTR in transduced A549 cells (cells with both red and green staining). Of note, the overall intensity of green fluorescence was also reduced in cells exposed to nicotine compared to no nicotine-treated cells, implying a suppression of endogenous CFTR in nicotine-treated cells. Insets were the enlarged boxed regions of respective images. Bars: 50 μm.
    Alexa Fluor 588 Conjugated Donkey Antirabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 588 conjugated donkey antirabbit igg/product/Jackson Immuno
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 588 conjugated donkey antirabbit igg - by Bioz Stars, 2020-08
    93/100 stars
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    97
    Jackson Immuno fluorescein fitc conjugated goat antirabbit igg secondary
    DcpS localizes to the nucleus and cytoplasm. ( A ) Endogenous DcpS was visualized in HeLa cells by indirect immunofluorescence microscopy using affinity-purified DcpS-specific rabbit antibody detected by <t>FITC-conjugated</t> goat <t>antirabbit</t> secondary antibody.
    Fluorescein Fitc Conjugated Goat Antirabbit Igg Secondary, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein fitc conjugated goat antirabbit igg secondary/product/Jackson Immuno
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescein fitc conjugated goat antirabbit igg secondary - by Bioz Stars, 2020-08
    97/100 stars
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    97
    Jackson Immuno goat antirabbit igg conjugated to hrp
    Analysis of serum levels of mouse C1q and rabbit <t>IgG</t> at 24 h after injection. Assessment of serum levels of C1q (a) and rabbit IgG (c) of control mice and in sera of mice sacrificed 24 h after injection with anti-C1q or IgG. The mean and standard deviation are shown ( n = 5 mice per group). (b) Western blot analysis of mouse sera, obtained 24 h after injection, of mice injected with anti-C1q or control rabbit IgG with NMS as a positive and C1q -/- as a negative control. Total serum was separated on a 10% SDS-PAGE gel under reducing conditions, blotted onto nitrocellulose and stained with Dig-conjugated rabbit antimouse C1q followed by goat <t>anti-Dig-HRP.</t> □, Anti-C1q; , control IgG.
    Goat Antirabbit Igg Conjugated To Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 97/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat antirabbit igg conjugated to hrp/product/Jackson Immuno
    Average 97 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    goat antirabbit igg conjugated to hrp - by Bioz Stars, 2020-08
    97/100 stars
      Buy from Supplier

    Image Search Results


    Immunolabeling of opsins in Moorfields/Institute of Ophthalmology-Müller 1 (MIO-M1) cells. A : Labeling of blue opsin and melanopsin, respectively, in MIO-M1 cells. The cells were counterstained against glutamine synthetase (GS) and GFAP, respectively. Double labeling yielded a yellow-orange merge signal. B : Labeling of rhodopsin and red-green opsin, respectively, in MIO-M1 cells. The cells were counterstained against GFAP. C : Control culture of MIO-M1 cells that was stained with secondary antibodies (goat antirabbit IgG and goat antimouse IgG) and without primary antibodies. No nonspecific labeling was observed either, following incubation with goat antirat IgG (not shown). D: Immunostaining of HEK-293 cells against melanopsin, blue opsin, and red green opsin, respectively. The cells were counterstained against glutamine synthetase (GS). Cell nuclei were labeled with the DNA dye Hoechst 33258 (blue). Bars are 20 µm.

    Journal: Molecular Vision

    Article Title: The human M?ller cell line MIO-M1 expresses opsins

    doi:

    Figure Lengend Snippet: Immunolabeling of opsins in Moorfields/Institute of Ophthalmology-Müller 1 (MIO-M1) cells. A : Labeling of blue opsin and melanopsin, respectively, in MIO-M1 cells. The cells were counterstained against glutamine synthetase (GS) and GFAP, respectively. Double labeling yielded a yellow-orange merge signal. B : Labeling of rhodopsin and red-green opsin, respectively, in MIO-M1 cells. The cells were counterstained against GFAP. C : Control culture of MIO-M1 cells that was stained with secondary antibodies (goat antirabbit IgG and goat antimouse IgG) and without primary antibodies. No nonspecific labeling was observed either, following incubation with goat antirat IgG (not shown). D: Immunostaining of HEK-293 cells against melanopsin, blue opsin, and red green opsin, respectively. The cells were counterstained against glutamine synthetase (GS). Cell nuclei were labeled with the DNA dye Hoechst 33258 (blue). Bars are 20 µm.

    Article Snippet: The following antibodies were used: rabbit anti-blue opsin (1:200; Millipore, Schwalbach, Germany), rabbit anti-red-green opsin (1:200; Millipore), monoclonal rat anti-rhodopsin (clone 5G6; 1:200; kindly provided by Stefanie M. Hauck, Dept. of Protein Sciences, Helmholtz Center, Munich, Germany), rabbit anti-melanopsin (1:200; Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-glutamine synthetase (1:500; clone GS-6; Millipore), mouse anti-glial fibrillary acidic protein (GFAP; 1:200; clone Ga5; Sigma-Aldrich, Taufkirchen, Germany), Cy2-coupled goat antirabbit IgG (1:400; Jackson ImmunoResearch, Newmarket, UK), Cy2-coupled goat antirat IgG (1:400; Jackson ImmunoResearch Laboratories), and Cy3-coupled goat antimouse IgG (1:400; Jackson ImmunoResearch).

    Techniques: Immunolabeling, Labeling, Staining, Incubation, Immunostaining

    Nicotine inhibits cystic fibrosis transmembrane conductance regulator (CFTR) protein in A549 cells characterized by an immunofluorescent assay. A549 cells were transfected with pacAd5.huCFTR-ECL4HA plasmid for 12 hours and cultured in medium with or without 200 nm/L of nicotine for additional 24 hours. The cells were then used for assessing CFTR protein and hemagglutinin (HA) with mouse anti-CFTR antibody (1:100) and rabbit polyclonal antibody to HA (1:100), respectively. Alexa Fluor 488-labeled donkey-anti-mouse immunoglobulin G (IgG) secondary antibody (green) and Alexa Fluor 588-conjugated donkey-anti-rabbit IgG antibody (red) were used as secondary antibodies for visualizing respective proteins of interest. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. A-F, Representative images showed that nicotine was able to suppress the abundance of both membrane and cytoplasmic CFTR in transduced A549 cells (cells with both red and green staining). Of note, the overall intensity of green fluorescence was also reduced in cells exposed to nicotine compared to no nicotine-treated cells, implying a suppression of endogenous CFTR in nicotine-treated cells. Insets were the enlarged boxed regions of respective images. Bars: 50 μm.

    Journal: Technology in Cancer Research & Treatment

    Article Title: Nicotine Induces Progressive Properties of Lung Adenocarcinoma A549 Cells by Inhibiting Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) Expression and Plasma Membrane Localization

    doi: 10.1177/1533033818809984

    Figure Lengend Snippet: Nicotine inhibits cystic fibrosis transmembrane conductance regulator (CFTR) protein in A549 cells characterized by an immunofluorescent assay. A549 cells were transfected with pacAd5.huCFTR-ECL4HA plasmid for 12 hours and cultured in medium with or without 200 nm/L of nicotine for additional 24 hours. The cells were then used for assessing CFTR protein and hemagglutinin (HA) with mouse anti-CFTR antibody (1:100) and rabbit polyclonal antibody to HA (1:100), respectively. Alexa Fluor 488-labeled donkey-anti-mouse immunoglobulin G (IgG) secondary antibody (green) and Alexa Fluor 588-conjugated donkey-anti-rabbit IgG antibody (red) were used as secondary antibodies for visualizing respective proteins of interest. 4′,6-diamidino-2-phenylindole (DAPI) was used for nuclear staining. A-F, Representative images showed that nicotine was able to suppress the abundance of both membrane and cytoplasmic CFTR in transduced A549 cells (cells with both red and green staining). Of note, the overall intensity of green fluorescence was also reduced in cells exposed to nicotine compared to no nicotine-treated cells, implying a suppression of endogenous CFTR in nicotine-treated cells. Insets were the enlarged boxed regions of respective images. Bars: 50 μm.

    Article Snippet: The primary antibody binding was detected using the Alexa Fluor 488-labeled donkey-anti-mouse immunoglobulin G (IgG) secondary antibody (1:500) and Alexa Fluor 588-conjugated donkey antirabbit IgG (1:500; Jackson ImmunoResearch Lab, West Grove, Pennsylvania).

    Techniques: Transfection, Plasmid Preparation, Cell Culture, Labeling, Staining, Fluorescence

    DcpS localizes to the nucleus and cytoplasm. ( A ) Endogenous DcpS was visualized in HeLa cells by indirect immunofluorescence microscopy using affinity-purified DcpS-specific rabbit antibody detected by FITC-conjugated goat antirabbit secondary antibody.

    Journal: RNA

    Article Title: Functional analysis of mRNA scavenger decapping enzymes

    doi: 10.1261/rna.7660804

    Figure Lengend Snippet: DcpS localizes to the nucleus and cytoplasm. ( A ) Endogenous DcpS was visualized in HeLa cells by indirect immunofluorescence microscopy using affinity-purified DcpS-specific rabbit antibody detected by FITC-conjugated goat antirabbit secondary antibody.

    Article Snippet: The secondary antibody consisted of fluorescein (FITC)-conjugated goat antirabbit IgG secondary (Jackson ImmunoResearch) antibody at a 1:80 dilution.

    Techniques: Immunofluorescence, Microscopy, Affinity Purification

    Analysis of serum levels of mouse C1q and rabbit IgG at 24 h after injection. Assessment of serum levels of C1q (a) and rabbit IgG (c) of control mice and in sera of mice sacrificed 24 h after injection with anti-C1q or IgG. The mean and standard deviation are shown ( n = 5 mice per group). (b) Western blot analysis of mouse sera, obtained 24 h after injection, of mice injected with anti-C1q or control rabbit IgG with NMS as a positive and C1q -/- as a negative control. Total serum was separated on a 10% SDS-PAGE gel under reducing conditions, blotted onto nitrocellulose and stained with Dig-conjugated rabbit antimouse C1q followed by goat anti-Dig-HRP. □, Anti-C1q; , control IgG.

    Journal: Clinical and Experimental Immunology

    Article Title: Glomerular deposition of C1q and anti-C1q antibodies in mice following injection of antimouse C1q antibodies

    doi: 10.1046/j.1365-2249.2003.02108.x

    Figure Lengend Snippet: Analysis of serum levels of mouse C1q and rabbit IgG at 24 h after injection. Assessment of serum levels of C1q (a) and rabbit IgG (c) of control mice and in sera of mice sacrificed 24 h after injection with anti-C1q or IgG. The mean and standard deviation are shown ( n = 5 mice per group). (b) Western blot analysis of mouse sera, obtained 24 h after injection, of mice injected with anti-C1q or control rabbit IgG with NMS as a positive and C1q -/- as a negative control. Total serum was separated on a 10% SDS-PAGE gel under reducing conditions, blotted onto nitrocellulose and stained with Dig-conjugated rabbit antimouse C1q followed by goat anti-Dig-HRP. □, Anti-C1q; , control IgG.

    Article Snippet: After washing, rabbit IgG binding was detected using goat antirabbit IgG conjugated to HRP (Jackson) in PBS-T-BSA containing 1 m NaCl.

    Techniques: Injection, Mouse Assay, Standard Deviation, Western Blot, Negative Control, SDS Page, Staining