antirabbit igg  (Cell Signaling Technology Inc)

 
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  • 96
    Name:
    Anti rabbit IgG H L F ab 2 Fragment PE Conjugate
    Description:
    Anti rabbit IgG H L F ab 2 Fragment was conjugated to phycoerythrin PE under optimal conditions and formulated at 1 mg ml This F ab 2 fragment results in less non specific binding to cells through Fc receptors
    Catalog Number:
    8885
    Price:
    None
    Applications:
    Flow Cytometry
    Category:
    Secondary Antibodies
    Buy from Supplier


    Structured Review

    Cell Signaling Technology Inc antirabbit igg
    Anti rabbit IgG H L F ab 2 Fragment was conjugated to phycoerythrin PE under optimal conditions and formulated at 1 mg ml This F ab 2 fragment results in less non specific binding to cells through Fc receptors
    https://www.bioz.com/result/antirabbit igg/product/Cell Signaling Technology Inc
    Average 96 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    antirabbit igg - by Bioz Stars, 2020-08
    96/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Mitochondrial Crisis in Cerebrovascular Endothelial Cells Opens the Blood-Brain Barrier
    Article Snippet: .. We stained cells with anti-rabbit-NADH-dehydrogenase-ubiquinone-1-alpha-subcomplex-assembly-factor-1 (NDUFAF1, sc-292085, 1:100, Santa Crutz), anti-mouse-NADH-dehydrogenase-ubiquinone-1-subunit-C2 (NDUFC2, sc-393771, 1:100, Santa Crutz), anti-mouse-NADH-dehydrogenase-ubiquinone-ironsulfur-protein-2 (NDUFS2, sc-390596, 1:100, Santa Crutz), anti-rabbit-Succinate-dehydrogenase (SDH, cat.11998, 1:100, Cell signaling, Beverly, Massachusetts), anti-rabbit-Cytochrome c (Cyc, cat.4280, 1:100, Cell signaling), anti-mouse-Cytochrome c oxidase (COX IV, cat. 4850, 1:100, Cell signaling) antibodies for 30 min and labeled the cells with a proper second antibody, PE-anti-rabbit (Cat.8885, 1:100, Cell signaling) or PE-anti-mouse (Cat.8887, 1:500, Cell signaling). .. We acquired data on BD Calibur flow cytometry and analyzed mean fluorescence intensity by Flowjo software.

    Incubation:

    Article Title: Bacterial Endotoxin Induces Oxidative Stress and Reduces Milk Protein Expression and Hypoxia in the Mouse Mammary Gland
    Article Snippet: .. Blots were washed 3 times with TBS-T for 10 min intervals followed by incubation with secondary antibodies [goat horseradish peroxidase- (HRP-) linked antirabbit IgG: #CST-7074P2, Cell Signaling; goat HRP-linked antimouse IgG: #sc-2005, Santa Cruz Biotechnology] at 1: 10,000 dilution and room temperature for 1 h. Blots were then washed 4 times in TBS in 10 min intervals before incubation with ECL-substrate solutions (#34577, Invitrogen). .. Blots were then imaged with ChemiDoc ECL Imager (Bio-Rad), stripped for 15 min in Restore Western Blot Stripping Buffer (#21063, Invitrogen), and reprobed with antibody for GAPDH (#2118, Cell Signaling; 1 : 5000).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Mitochondrial Crisis in Cerebrovascular Endothelial Cells Opens the Blood-Brain Barrier
    Article Snippet: .. We stained cells with anti-rabbit-NADH-dehydrogenase-ubiquinone-1-alpha-subcomplex-assembly-factor-1 (NDUFAF1, sc-292085, 1:100, Santa Crutz), anti-mouse-NADH-dehydrogenase-ubiquinone-1-subunit-C2 (NDUFC2, sc-393771, 1:100, Santa Crutz), anti-mouse-NADH-dehydrogenase-ubiquinone-ironsulfur-protein-2 (NDUFS2, sc-390596, 1:100, Santa Crutz), anti-rabbit-Succinate-dehydrogenase (SDH, cat.11998, 1:100, Cell signaling, Beverly, Massachusetts), anti-rabbit-Cytochrome c (Cyc, cat.4280, 1:100, Cell signaling), anti-mouse-Cytochrome c oxidase (COX IV, cat. 4850, 1:100, Cell signaling) antibodies for 30 min and labeled the cells with a proper second antibody, PE-anti-rabbit (Cat.8885, 1:100, Cell signaling) or PE-anti-mouse (Cat.8887, 1:500, Cell signaling). .. We acquired data on BD Calibur flow cytometry and analyzed mean fluorescence intensity by Flowjo software.

    Labeling:

    Article Title: Memory enhancement and protective effects of crocin against D-galactose aging model in the hippocampus of Wistar rats
    Article Snippet: .. Mouse monoclonal beta actin and phospho-p44/42 MAPK (pErk1/2), rabbit monoclonal NF-κB p65, Akt, phospho Akt, p44/42 MAPK (Erk1/2) and antirabbit IgG labeled with horseradish peroxidase were purchased from Cell Signaling. .. Polyvinylidene fluoride (PVDF) membrane was provided from Bio-Rad.

    Article Title: Mitochondrial Crisis in Cerebrovascular Endothelial Cells Opens the Blood-Brain Barrier
    Article Snippet: .. We stained cells with anti-rabbit-NADH-dehydrogenase-ubiquinone-1-alpha-subcomplex-assembly-factor-1 (NDUFAF1, sc-292085, 1:100, Santa Crutz), anti-mouse-NADH-dehydrogenase-ubiquinone-1-subunit-C2 (NDUFC2, sc-393771, 1:100, Santa Crutz), anti-mouse-NADH-dehydrogenase-ubiquinone-ironsulfur-protein-2 (NDUFS2, sc-390596, 1:100, Santa Crutz), anti-rabbit-Succinate-dehydrogenase (SDH, cat.11998, 1:100, Cell signaling, Beverly, Massachusetts), anti-rabbit-Cytochrome c (Cyc, cat.4280, 1:100, Cell signaling), anti-mouse-Cytochrome c oxidase (COX IV, cat. 4850, 1:100, Cell signaling) antibodies for 30 min and labeled the cells with a proper second antibody, PE-anti-rabbit (Cat.8885, 1:100, Cell signaling) or PE-anti-mouse (Cat.8887, 1:500, Cell signaling). .. We acquired data on BD Calibur flow cytometry and analyzed mean fluorescence intensity by Flowjo software.

    Article Title: Involvement of brain-derived neurotrophic factor (BDNF) on malathion induced depressive-like behavior in subacute exposure and protective effects of crocin
    Article Snippet: .. Antirabbit IgG labeled with horseradish peroxidase were purchased from Cell Signaling. .. BradFord Protein assay kit (#500-0002) and polyvinylidene fluoride (PVDF) membrane were purchased from BioRad (#162-0177).

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  • 96
    Cell Signaling Technology Inc antirabbit igg alexa 647
    (A) Confocal microscopy; HCT116 cells were stained with DAPI after treatment with 20 μM TriplatinNC for 24 h and visualized by confocal microscopy. (B) (Top) Mitotic index assay; image of (1) early pro-metaphase, (2) late pro-metaphase, (3) anaphase, (4) late anaphase/telophase, and (5) interphase as determined by DAPI DNA stain (blue); β-tubulin (red); and nucleophosmin/B23 (yellow) immunostaining. (Bottom) The mitotic index was derived as the number of cells in all mitotic phases combined (P + M + A + T) and divided by the total number of cells. n > 500 cells per time treatment ( > 1000 cells counted total for two repeat experiments). (C) Mitotic checkpoint assay; HCT116 were treated with or without 20 μM TriplatinNC, fixed, and incubated with phospho-histone H3 (Ser10), followed by <t>antirabbit</t> <t>Alexa</t> 647 secondary antibody and PI staining. Ten thousand events were analyzed by flow cytometry. Shown is a representative of two independent experiments.
    Antirabbit Igg Alexa 647, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirabbit igg alexa 647/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antirabbit igg alexa 647 - by Bioz Stars, 2020-08
    96/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc fluorochrome conjugated antirabbit secondary antibodies
    Colocalization of NNTs with lysosome (a–d) and autophagosome (e–h) in HEK 293 cells. Representative confocal laser scanning microscopy images after 48 h of cells incubated with media only (a, e), Lumafluor Red (b, f), or Rhodamine-NNT (c, g) (see Figures S13–S16 and S18–S21 for other NNTs). Scale bars = 10 μm. Percent colocalization with the lysosomes (d) and autophagosomes (h) was quantified from confocal images. Data is presented as mean ± SD ( n = 3). Nuclei of HEK 293 cells were stained with DAPI (blue). In parts a–c, lysosomes were stained with LysoTracker Green DND-26 (green). In parts e–g, autophagosomes were immunostained with LC3 antibody with <t>Antirabbit</t> IgG (H+L), F(ab′)2 Fragment conjugated with Alexa Fluor(R) 488. Colocalization is depicted in orange (red and green merge).
    Fluorochrome Conjugated Antirabbit Secondary Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorochrome conjugated antirabbit secondary antibodies/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorochrome conjugated antirabbit secondary antibodies - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    Cell Signaling Technology Inc goat antirabbit igg h l
    Colocalization of NNTs with lysosome (a–d) and autophagosome (e–h) in HEK 293 cells. Representative confocal laser scanning microscopy images after 48 h of cells incubated with media only (a, e), Lumafluor Red (b, f), or Rhodamine-NNT (c, g) (see Figures S13–S16 and S18–S21 for other NNTs). Scale bars = 10 μm. Percent colocalization with the lysosomes (d) and autophagosomes (h) was quantified from confocal images. Data is presented as mean ± SD ( n = 3). Nuclei of HEK 293 cells were stained with DAPI (blue). In parts a–c, lysosomes were stained with LysoTracker Green DND-26 (green). In parts e–g, autophagosomes were immunostained with LC3 antibody with <t>Antirabbit</t> IgG (H+L), F(ab′)2 Fragment conjugated with Alexa Fluor(R) 488. Colocalization is depicted in orange (red and green merge).
    Goat Antirabbit Igg H L, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat antirabbit igg h l/product/Cell Signaling Technology Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    goat antirabbit igg h l - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    (A) Confocal microscopy; HCT116 cells were stained with DAPI after treatment with 20 μM TriplatinNC for 24 h and visualized by confocal microscopy. (B) (Top) Mitotic index assay; image of (1) early pro-metaphase, (2) late pro-metaphase, (3) anaphase, (4) late anaphase/telophase, and (5) interphase as determined by DAPI DNA stain (blue); β-tubulin (red); and nucleophosmin/B23 (yellow) immunostaining. (Bottom) The mitotic index was derived as the number of cells in all mitotic phases combined (P + M + A + T) and divided by the total number of cells. n > 500 cells per time treatment ( > 1000 cells counted total for two repeat experiments). (C) Mitotic checkpoint assay; HCT116 were treated with or without 20 μM TriplatinNC, fixed, and incubated with phospho-histone H3 (Ser10), followed by antirabbit Alexa 647 secondary antibody and PI staining. Ten thousand events were analyzed by flow cytometry. Shown is a representative of two independent experiments.

    Journal: Molecular Pharmaceutics

    Article Title: Nucleolar Targeting by Platinum: p53-Independent Apoptosis Follows rRNA Inhibition, Cell-Cycle Arrest, and DNA Compaction

    doi: 10.1021/mp5006867

    Figure Lengend Snippet: (A) Confocal microscopy; HCT116 cells were stained with DAPI after treatment with 20 μM TriplatinNC for 24 h and visualized by confocal microscopy. (B) (Top) Mitotic index assay; image of (1) early pro-metaphase, (2) late pro-metaphase, (3) anaphase, (4) late anaphase/telophase, and (5) interphase as determined by DAPI DNA stain (blue); β-tubulin (red); and nucleophosmin/B23 (yellow) immunostaining. (Bottom) The mitotic index was derived as the number of cells in all mitotic phases combined (P + M + A + T) and divided by the total number of cells. n > 500 cells per time treatment ( > 1000 cells counted total for two repeat experiments). (C) Mitotic checkpoint assay; HCT116 were treated with or without 20 μM TriplatinNC, fixed, and incubated with phospho-histone H3 (Ser10), followed by antirabbit Alexa 647 secondary antibody and PI staining. Ten thousand events were analyzed by flow cytometry. Shown is a representative of two independent experiments.

    Article Snippet: A 1:500 dilution of antirabbit IgG Alexa 647 (Cell Signaling, #4414) or antimouse Alexa 555 (Cell Signaling, #4409) secondary antibody conjugates was added for 3 h at room temperature.

    Techniques: Confocal Microscopy, Staining, Immunostaining, Derivative Assay, Incubation, Flow Cytometry, Cytometry

    Colocalization of NNTs with lysosome (a–d) and autophagosome (e–h) in HEK 293 cells. Representative confocal laser scanning microscopy images after 48 h of cells incubated with media only (a, e), Lumafluor Red (b, f), or Rhodamine-NNT (c, g) (see Figures S13–S16 and S18–S21 for other NNTs). Scale bars = 10 μm. Percent colocalization with the lysosomes (d) and autophagosomes (h) was quantified from confocal images. Data is presented as mean ± SD ( n = 3). Nuclei of HEK 293 cells were stained with DAPI (blue). In parts a–c, lysosomes were stained with LysoTracker Green DND-26 (green). In parts e–g, autophagosomes were immunostained with LC3 antibody with Antirabbit IgG (H+L), F(ab′)2 Fragment conjugated with Alexa Fluor(R) 488. Colocalization is depicted in orange (red and green merge).

    Journal: ACS Central Science

    Article Title: Multicolor Polymeric Nanoparticle Neuronal Tracers

    doi: 10.1021/acscentsci.0c00027

    Figure Lengend Snippet: Colocalization of NNTs with lysosome (a–d) and autophagosome (e–h) in HEK 293 cells. Representative confocal laser scanning microscopy images after 48 h of cells incubated with media only (a, e), Lumafluor Red (b, f), or Rhodamine-NNT (c, g) (see Figures S13–S16 and S18–S21 for other NNTs). Scale bars = 10 μm. Percent colocalization with the lysosomes (d) and autophagosomes (h) was quantified from confocal images. Data is presented as mean ± SD ( n = 3). Nuclei of HEK 293 cells were stained with DAPI (blue). In parts a–c, lysosomes were stained with LysoTracker Green DND-26 (green). In parts e–g, autophagosomes were immunostained with LC3 antibody with Antirabbit IgG (H+L), F(ab′)2 Fragment conjugated with Alexa Fluor(R) 488. Colocalization is depicted in orange (red and green merge).

    Article Snippet: After washing, cells were incubated in fluorochrome-conjugated antirabbit secondary antibodies (Anti-Rabbit IgG (H+L), F(ab′)2 Fragment (Alexa Fluor 488 Conjugate) or Anti-Rabbit IgG (H+L), F(ab′)2 Fragment (Alexa Fluor 555 Conjugate)) diluted in antibody dilution buffer (1:500; Cell Signaling Technology) for 2 h at room temperature in the dark.

    Techniques: Confocal Laser Scanning Microscopy, Incubation, Staining