antirabbit igg antibody  (Abcam)

 
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  • 96
    Name:
    Anti Rabbit IgG Fc antibody RMG02
    Description:

    Catalog Number:
    ab190492
    Price:
    None
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    Structured Review

    Abcam antirabbit igg antibody

    https://www.bioz.com/result/antirabbit igg antibody/product/Abcam
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antirabbit igg antibody - by Bioz Stars, 2020-08
    96/100 stars

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    Related Articles

    Negative Control:

    Article Title: The Transcriptional Coactivator Cbp Regulates Self-Renewal and Differentiation in Adult Hematopoietic Stem Cells ▿
    Article Snippet: .. Immunoprecipitation was performed using rabbit polyclonal Cbp antibody (ab2832; Abcam) and rabbit IgG antibody (Abcam) as a negative control as described previously ( ). .. Each sample was amplified and sequenced using an Illumina Genome Analyzer IIx.

    Infection:

    Article Title: Human Autoantibodies Specific for Neurotrophin Receptors TrkA, TrkB, and TrkC Protect against Lethal Trypanosoma cruzi Infection in Mice
    Article Snippet: .. For inhibition of infection, cells were pre-incubated with ATA, control antibodies, or a rabbit IgG antibody specific for the extracellular domain of TrkA (Abcam, Cambridge, MA) in 1% bovine serum albumin/ Dulbecco’s modified Eagle’s medium for 60 minutes followed by parasites (Silvio or Tulahuen strain) in the same medium for 2 hours, and parasites that did not attach or invade cells were removed by washing the monolayers. .. Monolayers, which contain infected and non-infected cells, were maintained at 37°C in complete medium (2.5% fetal calf serum in Dulbecco’s modified Eagle’s medium) 2 or 3 days before fixation and staining.

    Immunoprecipitation:

    Article Title: The Transcriptional Coactivator Cbp Regulates Self-Renewal and Differentiation in Adult Hematopoietic Stem Cells ▿
    Article Snippet: .. Immunoprecipitation was performed using rabbit polyclonal Cbp antibody (ab2832; Abcam) and rabbit IgG antibody (Abcam) as a negative control as described previously ( ). .. Each sample was amplified and sequenced using an Illumina Genome Analyzer IIx.

    Incubation:

    Article Title: The LPS Responsiveness in BN and LEW Rats and Its Severity Are Modulated by the Liver
    Article Snippet: .. The membrane was incubated for 1 h at room temperature with a goat polyclonal to rabbit IgG antibody (1 : 10000, Abcam) and developed with the SuperSignal West Femto Trial kit (Thermo, USA). .. The membrane was exposed to high-sensitivity films (GE Healthcare) for autoradiography.

    Article Title: PRPF8 is important for BRCA1-mediated homologous recombination
    Article Snippet: .. Soluble material was pre-cleared with protein-G Dynabeads (Novex 10003D) prior to incubation with 2 μg of PRPF8 antibody (Bethyl A303-921A-T), BRCA1 (Millipore 07-434), Flag antibody (Sigma catalog F3165), or IgG rabbit antibody (Abcam 27478), followed by addition of protein-G Dynabeads, which were washed with IP buffer, eluted with 100 mM Glycine pH 2.5, and neutralized with 1 M Tris-HCl pH 10.85. .. Blots of these extracts or IPs were probed with antibodies described above for the microscopy analysis, as well as H4K16Ac (Epigentek A4030), H3K9Ac (Upstate 06-942), H3K9me3 (Upstate 07-442), POL2-S2P (Abcam ab5095), Actin (Sigma A2066), and HRP-conjugated secondary antibodies (Santa Cruz Biotech, sc-2004, sc-2005, sc-2020).

    Article Title: Replication confers β cell immaturity
    Article Snippet: .. 1/20 of sheared chromatin was kept aside as input and the remaining chromatin was incubated overnight with 1 µg rabbit IgG antibody (provided in kit) or 3 µg anti c-Myc antibody (ab56, Abcam). .. Antibody-chromatin complexes were pulled down using Protein A-coated magnetic beads and de-crosslinked for 4 h. The DNA was purified using MicroChIP DiaPure columns (Diagenode) and quantitative PCR performed using the FastStart Universal SYBR Green Master (Roche).

    Inhibition:

    Article Title: Human Autoantibodies Specific for Neurotrophin Receptors TrkA, TrkB, and TrkC Protect against Lethal Trypanosoma cruzi Infection in Mice
    Article Snippet: .. For inhibition of infection, cells were pre-incubated with ATA, control antibodies, or a rabbit IgG antibody specific for the extracellular domain of TrkA (Abcam, Cambridge, MA) in 1% bovine serum albumin/ Dulbecco’s modified Eagle’s medium for 60 minutes followed by parasites (Silvio or Tulahuen strain) in the same medium for 2 hours, and parasites that did not attach or invade cells were removed by washing the monolayers. .. Monolayers, which contain infected and non-infected cells, were maintained at 37°C in complete medium (2.5% fetal calf serum in Dulbecco’s modified Eagle’s medium) 2 or 3 days before fixation and staining.

    Modification:

    Article Title: Human Autoantibodies Specific for Neurotrophin Receptors TrkA, TrkB, and TrkC Protect against Lethal Trypanosoma cruzi Infection in Mice
    Article Snippet: .. For inhibition of infection, cells were pre-incubated with ATA, control antibodies, or a rabbit IgG antibody specific for the extracellular domain of TrkA (Abcam, Cambridge, MA) in 1% bovine serum albumin/ Dulbecco’s modified Eagle’s medium for 60 minutes followed by parasites (Silvio or Tulahuen strain) in the same medium for 2 hours, and parasites that did not attach or invade cells were removed by washing the monolayers. .. Monolayers, which contain infected and non-infected cells, were maintained at 37°C in complete medium (2.5% fetal calf serum in Dulbecco’s modified Eagle’s medium) 2 or 3 days before fixation and staining.

    other:

    Article Title: In vitro comparison of liposomal drug delivery systems targeting the oxytocin receptor: a potential novel treatment for obstetric complications
    Article Snippet: Rabbit IgG antibody and anti-OTR monoclonal antibody (ab181077) were obtained from Abcam (Cambridge, UK).

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  • 94
    Abcam polyclonal hrp conjugated goat antirabbit igg
    Antigenicity of immobilized native and His-tagged EcAII in ELISA. Titration of rabbit <t>polyclonal</t> anti-EcAII antibodies <t>(IgG)</t> in ELISA with native EcAII (black), N21-EcAII (green), N26-EcAII (blue), and tetrameric (red) or monomeric (red, dashed line) C8-EcAII. The data were fitted to a one-site binding model. The error bars show standard deviation for triplicate reads. (A) Proteins were randomly adsorbed onto Nunc-Maxisorp plates. Detection was performed with Fc-specific goat horse radish peroxidase <t>(HRP)-conjugated</t> <t>antirabbit</t> IgG (1:1000 dilution) and 0.16% H 2 O 2 . (B) ELISA with His-tagged variants immobilized in an oriented manner on Ni-NTA-coated microplates.
    Polyclonal Hrp Conjugated Goat Antirabbit Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal hrp conjugated goat antirabbit igg/product/Abcam
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal hrp conjugated goat antirabbit igg - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    Abcam antirabbit fitc conjugated goat igg
    Cell surface expression of insulin degrading enzyme (IDE) modulates ubiquitin-induced effects on cell signaling and function. (A) Quantification of IDE cell surface expression by flow cytometry. Thick lines: Cells labeled with rabbit <t>anti-IDE/antirabbit</t> <t>FITC</t> goat <t>IgG.</t> Thin lines: Control; cells labeled with rabbit IgG/antirabbit FITC goat IgG. Gray: Unstained cells. Red: BV-2 cells. Blue: BV-2 shRNA IDE cells. (B) FITC-ubiquitin binding (1 min, 4 °C) to BV-2 (●) and BV-2 shRNA IDE (○) cells. ●: nonspecific binding (NSB) - BV-2 cells. ○: nonspecific binding (NSB) - BV-2 shRNA cells. RFU: relative fluorescence units. (C) Forskolin stimulated BV-2 (gray bars) and BV-2 shRNA cells (open bars) were incubated with ubiquitin and cAMP levels were measured ( n = 6). *: p
    Antirabbit Fitc Conjugated Goat Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirabbit fitc conjugated goat igg/product/Abcam
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    antirabbit fitc conjugated goat igg - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Abcam fluorescein isothiocyanate labeled goat antirabbit immunoglobulin g antibody
    Cell surface expression of insulin degrading enzyme (IDE) modulates ubiquitin-induced effects on cell signaling and function. (A) Quantification of IDE cell surface expression by flow cytometry. Thick lines: Cells labeled with rabbit <t>anti-IDE/antirabbit</t> <t>FITC</t> goat <t>IgG.</t> Thin lines: Control; cells labeled with rabbit IgG/antirabbit FITC goat IgG. Gray: Unstained cells. Red: BV-2 cells. Blue: BV-2 shRNA IDE cells. (B) FITC-ubiquitin binding (1 min, 4 °C) to BV-2 (●) and BV-2 shRNA IDE (○) cells. ●: nonspecific binding (NSB) - BV-2 cells. ○: nonspecific binding (NSB) - BV-2 shRNA cells. RFU: relative fluorescence units. (C) Forskolin stimulated BV-2 (gray bars) and BV-2 shRNA cells (open bars) were incubated with ubiquitin and cAMP levels were measured ( n = 6). *: p
    Fluorescein Isothiocyanate Labeled Goat Antirabbit Immunoglobulin G Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescein isothiocyanate labeled goat antirabbit immunoglobulin g antibody/product/Abcam
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    fluorescein isothiocyanate labeled goat antirabbit immunoglobulin g antibody - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    Antigenicity of immobilized native and His-tagged EcAII in ELISA. Titration of rabbit polyclonal anti-EcAII antibodies (IgG) in ELISA with native EcAII (black), N21-EcAII (green), N26-EcAII (blue), and tetrameric (red) or monomeric (red, dashed line) C8-EcAII. The data were fitted to a one-site binding model. The error bars show standard deviation for triplicate reads. (A) Proteins were randomly adsorbed onto Nunc-Maxisorp plates. Detection was performed with Fc-specific goat horse radish peroxidase (HRP)-conjugated antirabbit IgG (1:1000 dilution) and 0.16% H 2 O 2 . (B) ELISA with His-tagged variants immobilized in an oriented manner on Ni-NTA-coated microplates.

    Journal: ACS Omega

    Article Title: Development of Escherichia coli Asparaginase II for Immunosensing: A Trade-Off between Receptor Density and Sensing Efficiency

    doi: 10.1021/acsomega.7b00110

    Figure Lengend Snippet: Antigenicity of immobilized native and His-tagged EcAII in ELISA. Titration of rabbit polyclonal anti-EcAII antibodies (IgG) in ELISA with native EcAII (black), N21-EcAII (green), N26-EcAII (blue), and tetrameric (red) or monomeric (red, dashed line) C8-EcAII. The data were fitted to a one-site binding model. The error bars show standard deviation for triplicate reads. (A) Proteins were randomly adsorbed onto Nunc-Maxisorp plates. Detection was performed with Fc-specific goat horse radish peroxidase (HRP)-conjugated antirabbit IgG (1:1000 dilution) and 0.16% H 2 O 2 . (B) ELISA with His-tagged variants immobilized in an oriented manner on Ni-NTA-coated microplates.

    Article Snippet: Polyclonal HRP-conjugated goat antirabbit IgG (Abcam, cat no. ab97200) at a dilution of (1:1000) in PBS was used for secondary detection.

    Techniques: Enzyme-linked Immunosorbent Assay, Titration, Binding Assay, Standard Deviation

    Cell surface expression of insulin degrading enzyme (IDE) modulates ubiquitin-induced effects on cell signaling and function. (A) Quantification of IDE cell surface expression by flow cytometry. Thick lines: Cells labeled with rabbit anti-IDE/antirabbit FITC goat IgG. Thin lines: Control; cells labeled with rabbit IgG/antirabbit FITC goat IgG. Gray: Unstained cells. Red: BV-2 cells. Blue: BV-2 shRNA IDE cells. (B) FITC-ubiquitin binding (1 min, 4 °C) to BV-2 (●) and BV-2 shRNA IDE (○) cells. ●: nonspecific binding (NSB) - BV-2 cells. ○: nonspecific binding (NSB) - BV-2 shRNA cells. RFU: relative fluorescence units. (C) Forskolin stimulated BV-2 (gray bars) and BV-2 shRNA cells (open bars) were incubated with ubiquitin and cAMP levels were measured ( n = 6). *: p

    Journal: Biochemistry

    Article Title: Modulation of the CXC Chemokine Receptor 4 Agonist Activity of Ubiquitin through C-Terminal Protein Modification

    doi: 10.1021/bi400254f

    Figure Lengend Snippet: Cell surface expression of insulin degrading enzyme (IDE) modulates ubiquitin-induced effects on cell signaling and function. (A) Quantification of IDE cell surface expression by flow cytometry. Thick lines: Cells labeled with rabbit anti-IDE/antirabbit FITC goat IgG. Thin lines: Control; cells labeled with rabbit IgG/antirabbit FITC goat IgG. Gray: Unstained cells. Red: BV-2 cells. Blue: BV-2 shRNA IDE cells. (B) FITC-ubiquitin binding (1 min, 4 °C) to BV-2 (●) and BV-2 shRNA IDE (○) cells. ●: nonspecific binding (NSB) - BV-2 cells. ○: nonspecific binding (NSB) - BV-2 shRNA cells. RFU: relative fluorescence units. (C) Forskolin stimulated BV-2 (gray bars) and BV-2 shRNA cells (open bars) were incubated with ubiquitin and cAMP levels were measured ( n = 6). *: p

    Article Snippet: Cells were labeled with polyclonal rabbit anti-IDE (ab32216, Abcam) in combination with antirabbit FITC conjugated goat IgG (Abcam).

    Techniques: Expressing, Flow Cytometry, Cytometry, Labeling, shRNA, Binding Assay, Fluorescence, Incubation