antirabbit alexa fluor 488 conjugate  (Jackson Immuno)

 
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    Name:
    Alexa Fluor 488 AffiniPure F ab ₂ Fragment Goat Anti Rabbit IgG F ab ₂ fragment specific
    Description:
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with the F ab 2 Fab portion of rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against the Fc portion of rabbit IgG or against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human serum proteins but it may cross react with immunoglobulins from other species
    Catalog Number:
    111-546-047
    Price:
    230.0
    Category:
    F ab ₂ Fragment Affinity Purified Antibodies
    Conjugate:
    Alexa Fluor 488
    Size:
    0 75 mg
    Format:
    F(ab')₂ Fragment
    Host:
    Goat
    Buy from Supplier


    Structured Review

    Jackson Immuno antirabbit alexa fluor 488 conjugate
    FOX1 localizes to the plasma membrane in iron-deprived Chlamydomonas cells and is oriented with the MCO domain outside the cell. The cell wall mutant strain, cc-425, was grown in TAP medium containing either 10 μM iron for FOX1 repression (A) or no iron plus 100 μM ferrozine for FOX1 induction (B). Cells were plated and left unpermeabilized prior to treatment with anti-FOX1 and <t>Alexa</t> <t>fluor</t> 488 conjugate antibodies. As a control for membrane integrity, permeabilized (C) and unpermeabilized cells (D) were probed for ATP synthase which is localized to the chloroplast. Dim fluorescence is visualized in the unpermeabilized cells (D), which is likely due to background autofluorescence. The bottom panels represent phase-contrast images of the same cells under each condition.
    F ab 2 fragment antibodies are generated by pepsin digestion of whole IgG antibodies to remove most of the Fc region while leaving some of the hinge region F ab 2 fragments have two antigen binding Fab portions linked together by disulfide bonds and therefore they are divalent The average molecular weight is about 110 kDa They are used for specific applications such as to avoid binding of secondary antibodies to live cells with Fc receptors or to Protein A or Protein G Based on immunoelectrophoresis and or ELISA the antibody reacts with the F ab 2 Fab portion of rabbit IgG It also reacts with the light chains of other rabbit immunoglobulins No antibody was detected against the Fc portion of rabbit IgG or against non immunoglobulin serum proteins The antibody has been tested by ELISA and or solid phase adsorbed to ensure minimal cross reaction with human serum proteins but it may cross react with immunoglobulins from other species
    https://www.bioz.com/result/antirabbit alexa fluor 488 conjugate/product/Jackson Immuno
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antirabbit alexa fluor 488 conjugate - by Bioz Stars, 2021-09
    98/100 stars

    Images

    1) Product Images from "Analysis of the High-Affinity Iron Uptake System at the Chlamydomonas reinhardtii Plasma Membrane ▿"

    Article Title: Analysis of the High-Affinity Iron Uptake System at the Chlamydomonas reinhardtii Plasma Membrane ▿

    Journal: Eukaryotic Cell

    doi: 10.1128/EC.00310-09

    FOX1 localizes to the plasma membrane in iron-deprived Chlamydomonas cells and is oriented with the MCO domain outside the cell. The cell wall mutant strain, cc-425, was grown in TAP medium containing either 10 μM iron for FOX1 repression (A) or no iron plus 100 μM ferrozine for FOX1 induction (B). Cells were plated and left unpermeabilized prior to treatment with anti-FOX1 and Alexa fluor 488 conjugate antibodies. As a control for membrane integrity, permeabilized (C) and unpermeabilized cells (D) were probed for ATP synthase which is localized to the chloroplast. Dim fluorescence is visualized in the unpermeabilized cells (D), which is likely due to background autofluorescence. The bottom panels represent phase-contrast images of the same cells under each condition.
    Figure Legend Snippet: FOX1 localizes to the plasma membrane in iron-deprived Chlamydomonas cells and is oriented with the MCO domain outside the cell. The cell wall mutant strain, cc-425, was grown in TAP medium containing either 10 μM iron for FOX1 repression (A) or no iron plus 100 μM ferrozine for FOX1 induction (B). Cells were plated and left unpermeabilized prior to treatment with anti-FOX1 and Alexa fluor 488 conjugate antibodies. As a control for membrane integrity, permeabilized (C) and unpermeabilized cells (D) were probed for ATP synthase which is localized to the chloroplast. Dim fluorescence is visualized in the unpermeabilized cells (D), which is likely due to background autofluorescence. The bottom panels represent phase-contrast images of the same cells under each condition.

    Techniques Used: Mutagenesis, Fluorescence

    Related Articles

    other:

    Article Title: Elevated glucosylsphingosine in Gaucher disease induced pluripotent stem cell neurons deregulates lysosomal compartment through mammalian target of rapamycin complex 1, et al. Elevated glucosylsphingosine in Gaucher disease induced pluripotent stem cell neurons deregulates lysosomal compartment through mammalian target of rapamycin complex 1
    Article Snippet: Secondary antibodies; Goat anti‐rabbit Alexa Fluor 488, Donkey anti‐chicken Alexa Fluor 647, and Donkey anti‐mouse Cy3 (Jackson ImmunoResearch Laboratories).

    Article Title: Elevated glucosylsphingosine in Gaucher disease induced pluripotent stem cell neurons deregulates lysosomal compartment through mammalian target of rapamycin complex 1.
    Article Snippet: Secondary antibodies; Goat anti-rabbit Alexa Fluor 488, Donkey anti-chicken Alexa Fluor 647, and Donkey anti-mouse Cy3 (Jackson ImmunoResearch Laboratories).

    Incubation:

    Article Title: Refining the Identity and Role of Kv4 Channels in Mouse Substantia Nigra Dopaminergic Neurons
    Article Snippet: .. After three washes (15 min/each) in PBS containing 0.3% Triton X-100, the floating sections were incubated with the following secondary antibodies: Alexa Fluor 488-goat anti-mouse (1:200, Jackson ImmunoResearch), Alexa Fluor 488-goat anti-rabbit (1:200, Jackson ImmunoResearch) and Alexa Fluor 594-goat anti-chicken (1:200, Jackson ImmunoResearch) in a PBS solution containing 0.3% Triton X-100 and 1% NGS for 2 h at RT. ..

    Article Title: Adjuvants and Vaccines Used in Allergen-Specific Immunotherapy Induce Neutrophil Extracellular Traps
    Article Snippet: .. After 3 washing steps for 5 min, coverslips were incubated for 1 h with 1:500 anti-rabbit Alexa Fluor 488 (Jackson Immuno Research Inc., West Grove, PA, USA) and anti-mouse Alexa Fluor 568 (Thermo Fisher Scientific). ..

    Article Title: Oral administration of repurposed drug targeting Cyp46A1 increases survival times of prion infected mice
    Article Snippet: .. After primary antibody incubation, the sections were washed twice for 5 min each and incubated for 1 hr with Alexa Fluor 488 goat anti-rabbit or Alexa FluorTM 555 goat anti-rabbit secondary antibodies (Jackson Immunoresearch) (1:100). ..

    Next-Generation Sequencing:

    Article Title: Refining the Identity and Role of Kv4 Channels in Mouse Substantia Nigra Dopaminergic Neurons
    Article Snippet: .. After three washes (15 min/each) in PBS containing 0.3% Triton X-100, the floating sections were incubated with the following secondary antibodies: Alexa Fluor 488-goat anti-mouse (1:200, Jackson ImmunoResearch), Alexa Fluor 488-goat anti-rabbit (1:200, Jackson ImmunoResearch) and Alexa Fluor 594-goat anti-chicken (1:200, Jackson ImmunoResearch) in a PBS solution containing 0.3% Triton X-100 and 1% NGS for 2 h at RT. ..

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  • 94
    Jackson Immuno alexa 488 conjugated antirabbit igg
    Association between TfR2 and caveolin-1 in lipid rafts as shown by immunofluorescence experiments. TB10 and U251 cells were plated on glass coverslips and left to adhere overnight. TfR-2 was visualized by incubating cells with anti-TfR_2 mAb and then with Texas Red-conjugated antimouse <t>IgG</t> (red fluorescence). Cells were then fixed, permeabilized, and then labeled with caveolin-1 antibody followed by Alexa 488-conjugated <t>antirabbit</t> antibody (green fluorescence). An image of a representative cells is shown reporting the red (TfR-2), green (Cav-1), merged fluorescence (at two different magnifications), and phase contrast.
    Alexa 488 Conjugated Antirabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa 488 conjugated antirabbit igg/product/Jackson Immuno
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa 488 conjugated antirabbit igg - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    98
    Jackson Immuno antirabbit alexa fluor 488 conjugate
    FOX1 localizes to the plasma membrane in iron-deprived Chlamydomonas cells and is oriented with the MCO domain outside the cell. The cell wall mutant strain, cc-425, was grown in TAP medium containing either 10 μM iron for FOX1 repression (A) or no iron plus 100 μM ferrozine for FOX1 induction (B). Cells were plated and left unpermeabilized prior to treatment with anti-FOX1 and <t>Alexa</t> <t>fluor</t> 488 conjugate antibodies. As a control for membrane integrity, permeabilized (C) and unpermeabilized cells (D) were probed for ATP synthase which is localized to the chloroplast. Dim fluorescence is visualized in the unpermeabilized cells (D), which is likely due to background autofluorescence. The bottom panels represent phase-contrast images of the same cells under each condition.
    Antirabbit Alexa Fluor 488 Conjugate, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirabbit alexa fluor 488 conjugate/product/Jackson Immuno
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antirabbit alexa fluor 488 conjugate - by Bioz Stars, 2021-09
    98/100 stars
      Buy from Supplier

    Image Search Results


    Association between TfR2 and caveolin-1 in lipid rafts as shown by immunofluorescence experiments. TB10 and U251 cells were plated on glass coverslips and left to adhere overnight. TfR-2 was visualized by incubating cells with anti-TfR_2 mAb and then with Texas Red-conjugated antimouse IgG (red fluorescence). Cells were then fixed, permeabilized, and then labeled with caveolin-1 antibody followed by Alexa 488-conjugated antirabbit antibody (green fluorescence). An image of a representative cells is shown reporting the red (TfR-2), green (Cav-1), merged fluorescence (at two different magnifications), and phase contrast.

    Journal: Translational Oncology

    Article Title: Transferrin Receptor 2 Is Frequently and Highly Expressed in Glioblastomas

    doi:

    Figure Lengend Snippet: Association between TfR2 and caveolin-1 in lipid rafts as shown by immunofluorescence experiments. TB10 and U251 cells were plated on glass coverslips and left to adhere overnight. TfR-2 was visualized by incubating cells with anti-TfR_2 mAb and then with Texas Red-conjugated antimouse IgG (red fluorescence). Cells were then fixed, permeabilized, and then labeled with caveolin-1 antibody followed by Alexa 488-conjugated antirabbit antibody (green fluorescence). An image of a representative cells is shown reporting the red (TfR-2), green (Cav-1), merged fluorescence (at two different magnifications), and phase contrast.

    Article Snippet: Cells were then fixed and permeabilized (4% paraformaldehyde/2% sucrose, 10 minutes at room temperature, plus 0.1% saponin, 30 minutes at room temperature), saturated (1% normal goat serum), and stained with a rabbit polyclonal anti-caveolin-1 (BD Bioscience, Bedford, MA), followed by an Alexa 488-conjugated antirabbit IgG (Jackson Immunoresearch).

    Techniques: Immunofluorescence, Fluorescence, Labeling

    FOX1 localizes to the plasma membrane in iron-deprived Chlamydomonas cells and is oriented with the MCO domain outside the cell. The cell wall mutant strain, cc-425, was grown in TAP medium containing either 10 μM iron for FOX1 repression (A) or no iron plus 100 μM ferrozine for FOX1 induction (B). Cells were plated and left unpermeabilized prior to treatment with anti-FOX1 and Alexa fluor 488 conjugate antibodies. As a control for membrane integrity, permeabilized (C) and unpermeabilized cells (D) were probed for ATP synthase which is localized to the chloroplast. Dim fluorescence is visualized in the unpermeabilized cells (D), which is likely due to background autofluorescence. The bottom panels represent phase-contrast images of the same cells under each condition.

    Journal: Eukaryotic Cell

    Article Title: Analysis of the High-Affinity Iron Uptake System at the Chlamydomonas reinhardtii Plasma Membrane ▿

    doi: 10.1128/EC.00310-09

    Figure Lengend Snippet: FOX1 localizes to the plasma membrane in iron-deprived Chlamydomonas cells and is oriented with the MCO domain outside the cell. The cell wall mutant strain, cc-425, was grown in TAP medium containing either 10 μM iron for FOX1 repression (A) or no iron plus 100 μM ferrozine for FOX1 induction (B). Cells were plated and left unpermeabilized prior to treatment with anti-FOX1 and Alexa fluor 488 conjugate antibodies. As a control for membrane integrity, permeabilized (C) and unpermeabilized cells (D) were probed for ATP synthase which is localized to the chloroplast. Dim fluorescence is visualized in the unpermeabilized cells (D), which is likely due to background autofluorescence. The bottom panels represent phase-contrast images of the same cells under each condition.

    Article Snippet: The secondary antibodies used were antirabbit Alexa Fluor 488 conjugate and antimouse Cy5 conjugate (Jackson Immuno Research), both diluted 1:500 in 3% BSA.

    Techniques: Mutagenesis, Fluorescence