antigen retrieval  (Abcam)

 
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    Name:
    Antigen Retrieval Buffer 100X EDTA Buffer pH 8 0
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    AB93680
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    Structured Review

    Abcam antigen retrieval

    https://www.bioz.com/result/antigen retrieval/product/Abcam
    Average 99 stars, based on 1 article reviews
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    antigen retrieval - by Bioz Stars, 2021-04
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    Related Articles

    Immunohistochemistry:

    Article Title: Nuclear Klf4 accumulation is associated with cetuximab drug-resistance and predicts poor prognosis of nasopharyngeal carcinoma
    Article Snippet: .. Immunohistochemistry (IHC) method After deparaffinization and rehydration, the paraffin-embedded sections were subjected to antigen retrieval for 2 min. Then, the slides were incubated overnight at 4 °C with the following primary antibodies: (dilution 1:200, abcam, MA). .. Serial tumor sections and IHC staining evaluated the expression of proteins.

    Article Title: An activin receptor IIA ligand trap corrects ineffective erythropoiesis in β-thalassemia
    Article Snippet: Immunohistology.Paraffin-embedded bone marrow or spleen sections (5 μm) were stained with H & E for morphological analysis. .. For immunohistochemistry and confocal analysis, sections were processed for antigen retrieval as indicated by each manufacturer and incubated overnight with primary antibodies against GDF11 proform (ab71347, Abcam; 1/100 dilution), mature GDF11 (sc28910, Santa Cruz; 1/20 dilution), activin A (AF338, R & D Systems; 1/100 dilution), activin B (MAB659, R & D Systems; 1/100 dilution), activin receptor II (sc-25451, Santa Cruz; 1/100 dilution), phospho-Smad1/5 (#9511, Cell Signaling; 1/100 dilution), phospho-Smad2 (#3101, Cell Signaling; 1/100 dilution), Ter-119 (#553673, BD Biosciences; 1/100 dilution), CD71 (136800, Life Technologies; 1/200 dilution) or F4/80 (sc-377009, Santa Cruz Biotechnology; 1/50 dilution). .. Following incubation with biotinylated species-specific secondary antibodies (1/250 dilution) for 1 h and streptavidin-HRP for 30 min (Vectastain, ABC kit; Vector Laboratories, Burlingame, USA), the immunoperoxidase reaction was visualized by addition of 3,3′-diaminobenzidine (DAB) in chromagen solution (Dako, Carpinteria, USA).

    Article Title: Ang-2 promotes lung cancer metastasis by increasing epithelial-mesenchymal transition
    Article Snippet: For analysis of cell migration, we use the modified Boyden Chambers without the Transwell-precoated Matrigel membrane filter with the method performed as above. .. Immunohistochemistry (IHC) TMA slides used for IHC analysis were deparaffinized, and eroxidase was quenched with methanol and 3% H2 O2 for 15 min. For antigen retrieval, the sections were boiled under pressure in citrate buffer (pH 6.0) for 3 min and then incubated for 120min with primary mouse anti-human Ang-2 (ab155106, Abcam, USA), washing with phosphate buffered saline (PBS), incubated with horse reddish peroxidase (HRP)-conjugated goat anti-mouse IgG (A21020; Abbkine, USA) for 15 min at 1:5000 dilution, and washed again with PBS. .. In finally, the slides were incubated with diaminobenzidine and counterstained with hematoxylin solution, dehydrated in ethanol, cleared in xylene, and cover-slipped.

    Incubation:

    Article Title: Nuclear Klf4 accumulation is associated with cetuximab drug-resistance and predicts poor prognosis of nasopharyngeal carcinoma
    Article Snippet: .. Immunohistochemistry (IHC) method After deparaffinization and rehydration, the paraffin-embedded sections were subjected to antigen retrieval for 2 min. Then, the slides were incubated overnight at 4 °C with the following primary antibodies: (dilution 1:200, abcam, MA). .. Serial tumor sections and IHC staining evaluated the expression of proteins.

    Article Title: An activin receptor IIA ligand trap corrects ineffective erythropoiesis in β-thalassemia
    Article Snippet: Immunohistology.Paraffin-embedded bone marrow or spleen sections (5 μm) were stained with H & E for morphological analysis. .. For immunohistochemistry and confocal analysis, sections were processed for antigen retrieval as indicated by each manufacturer and incubated overnight with primary antibodies against GDF11 proform (ab71347, Abcam; 1/100 dilution), mature GDF11 (sc28910, Santa Cruz; 1/20 dilution), activin A (AF338, R & D Systems; 1/100 dilution), activin B (MAB659, R & D Systems; 1/100 dilution), activin receptor II (sc-25451, Santa Cruz; 1/100 dilution), phospho-Smad1/5 (#9511, Cell Signaling; 1/100 dilution), phospho-Smad2 (#3101, Cell Signaling; 1/100 dilution), Ter-119 (#553673, BD Biosciences; 1/100 dilution), CD71 (136800, Life Technologies; 1/200 dilution) or F4/80 (sc-377009, Santa Cruz Biotechnology; 1/50 dilution). .. Following incubation with biotinylated species-specific secondary antibodies (1/250 dilution) for 1 h and streptavidin-HRP for 30 min (Vectastain, ABC kit; Vector Laboratories, Burlingame, USA), the immunoperoxidase reaction was visualized by addition of 3,3′-diaminobenzidine (DAB) in chromagen solution (Dako, Carpinteria, USA).

    Article Title: Ang-2 promotes lung cancer metastasis by increasing epithelial-mesenchymal transition
    Article Snippet: For analysis of cell migration, we use the modified Boyden Chambers without the Transwell-precoated Matrigel membrane filter with the method performed as above. .. Immunohistochemistry (IHC) TMA slides used for IHC analysis were deparaffinized, and eroxidase was quenched with methanol and 3% H2 O2 for 15 min. For antigen retrieval, the sections were boiled under pressure in citrate buffer (pH 6.0) for 3 min and then incubated for 120min with primary mouse anti-human Ang-2 (ab155106, Abcam, USA), washing with phosphate buffered saline (PBS), incubated with horse reddish peroxidase (HRP)-conjugated goat anti-mouse IgG (A21020; Abbkine, USA) for 15 min at 1:5000 dilution, and washed again with PBS. .. In finally, the slides were incubated with diaminobenzidine and counterstained with hematoxylin solution, dehydrated in ethanol, cleared in xylene, and cover-slipped.

    Article Title: Expression and prognostic significance of MYL9 in esophageal squamous cell carcinoma
    Article Snippet: Immunohistochemistry (IHC)Formalin-fixed, paraffin-embedded samples were cut into 5-μm sections and processed for IHC. .. Tissue sections prepared for antigen retrieval by microwave treatment in EDTA buffer (pH 9.0) were incubated with primary antibodies against MYL9 (1:1000; ab191393, Abcam) and α-smooth muscle actin (SMA) (1:500; Zhongshan Bio-Tech, Zhongshan, China). .. Immunostaining was performed using the EnVision System with diaminobenzidine (Dako Cytomation, Glostrup, Denmark).

    Article Title: Hereditary pancreatitis model by blastocyst complementation in mouse
    Article Snippet: The paraffin-embedded sections were de-paraffinized in Hemo-De (Farma, Japan) and were rehydrated in a graded series of ethanol. .. The slides were heated in an antigen retrieval buffer for 40 minutes, blocked with goat or horse serum for 20 minutes at room temperature, and incubated with monoclonal mouse anti-Gfp antigen antibody (1:1000, Abcam, England) or monoclonal mouse anti-PTF1A antigen antibody (1:50, BD Biosciences, US) overnight at 4°C. .. The Vectastain ABC System (Vectastain, Funakoshi, Japan) was used to visualize the antigens.

    Article Title: Leptin System in Obese Dog Skin: A Pilot Study
    Article Snippet: Immunohistochemistry Immunohistochemistry was carried out as follows [ ]: sections were rehydrated and dipped for 10 min in 3% H2 O2 to reduce endogenous peroxidase activity. .. To perform antigen retrieval, sections were microwaved for 15 min in 10 mM citric acid (pH 6.0) or incubated with Historeveal (Abcam, Cambridge, UK) for 5 min as anti-LEPR antibody regards. .. The sections were then blocked with 1:10 normal serum ( ) for 30 min and incubated at room temperature with the primary antibody ( ) for 1h (for anti-CD20, Anti-CD3 and anti-Iba-1 antibodies) or overnight (for anti-LEP and anti-LEPR antibodies).

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    Abcam phospho s1pr1
    Newly Generated DGCs Highly Express <t>S1PR1</t> (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p
    Phospho S1pr1, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Abcam caspase 3
    Molecular correlates of reoviral infection in cell preparations. Co-expression analysis of reovirus (red signal) and <t>caspase-3</t> (brown signal) ( a ) in the cell line CAL27 shows that many of the cells with signal show apoptotic changes. The insert shows
    Caspase 3, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caspase 3/product/Abcam
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    99
    Abcam anti cd11b primary antibody
    Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 <t>(CD11b)</t> (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)
    Anti Cd11b Primary Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Newly Generated DGCs Highly Express S1PR1 (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p

    Journal: Cell reports

    Article Title: Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain

    doi: 10.1016/j.celrep.2019.12.084

    Figure Lengend Snippet: Newly Generated DGCs Highly Express S1PR1 (A) (Top) Procedure for RNA-seq analysis of new neurons 1 day after differentiating from adult hippocampal neural stem cells. (Bottom) Expression profiling of sphingolipid signaling-related genes. Mann-Whitney U test, SPHK1 p = 0.064, S1PR1 p = 0.064, ACSL1 p = 0.064, ASAH1 p = 0.064, SMPD1 p = 0.064, DGAT1 p = 0.064, ACSS2 p = 0.355, SGPL1 p = 0.064, ALDH3A2 p = 0.04, SPNS2 p = 1.000, SGMS1 p = 0.064, S1PR2 p = 0.165, CERS2 p = 0.064, CERS5 p = 0.355, CERK p = 0.355, SPHK2 p = 0.064, SPTLC1 p = 1.000, FAR1 p = 0.064, ASAH2 p = 0.643, DEGS1 p = 0.060, RAC p = 0.165, CERS4 p = 0.064, FHL2 p =1.000, UGCG p = 0.06, ACER2 p = 0.643. (B) (Left) Region of dentate gyrus dissected. (Right) S1PR isoform mRNA detection (S1PR5 was not detected). Scale bar, 100 μm. ). (D) (Top) Retroviral GFP was delivered to the DG to label newly born DGCs. (Bottom) Representative image showing expression of S1PR1 in a DGC at 7 dpi. Scale bar, 10 μm. (E) Schematic of hippocampal/cortical dissection and lipidomics analysis. (F) Concentrations of various sphingolipid metabolites in several brain regions. DG versus Ctx: p

    Article Snippet: Immunohistochemistry was performed by blocking sections in 1% donkey serum in phosphate-buffered saline with 0.025% Triton for 1 h at room temperature and then incubating them overnight with shaking at 4°C with the following primary antibodies: GFP (rabbit polyclonal antibody, 1:1,000; Sigma-Aldrich, St. Louis, MO), Prox1 (mouse monoclonal, 1:500; Millipore, Burlington, MA), DCX (goat polyclonal antibody, 1:1,000; Santa Cruz Biotechnology, Dallas, TX), S1PR1 (rabbit polyclonal antibody, 1:500; Abcam, Cambridge, UK), phospho-S1PR1 (rabbit polyclonal antibody, 1:500, Abcam, Cambridge, UK- requires antigen retrieval protocol as previously described ( )).

    Techniques: Generated, RNA Sequencing Assay, Expressing, MANN-WHITNEY, Dissection

    S1PR1 Knockdown Reduces Neurite Growth and Horizontal-to-Radial Repositioning (A) Description of shRNA expression vector and experimental timeline of shRNA viral injection and doxycycline induction. (B) (Left) Representative images of S1PR1 expression in shS1PR1 + and shLuc + DGCs at 5 dpi. (Right) Summary plot of the S1PR1 fluorescent signal intensity. *p

    Journal: Cell reports

    Article Title: Circuit Integration Initiation of New Hippocampal Neurons in the Adult Brain

    doi: 10.1016/j.celrep.2019.12.084

    Figure Lengend Snippet: S1PR1 Knockdown Reduces Neurite Growth and Horizontal-to-Radial Repositioning (A) Description of shRNA expression vector and experimental timeline of shRNA viral injection and doxycycline induction. (B) (Left) Representative images of S1PR1 expression in shS1PR1 + and shLuc + DGCs at 5 dpi. (Right) Summary plot of the S1PR1 fluorescent signal intensity. *p

    Article Snippet: Immunohistochemistry was performed by blocking sections in 1% donkey serum in phosphate-buffered saline with 0.025% Triton for 1 h at room temperature and then incubating them overnight with shaking at 4°C with the following primary antibodies: GFP (rabbit polyclonal antibody, 1:1,000; Sigma-Aldrich, St. Louis, MO), Prox1 (mouse monoclonal, 1:500; Millipore, Burlington, MA), DCX (goat polyclonal antibody, 1:1,000; Santa Cruz Biotechnology, Dallas, TX), S1PR1 (rabbit polyclonal antibody, 1:500; Abcam, Cambridge, UK), phospho-S1PR1 (rabbit polyclonal antibody, 1:500, Abcam, Cambridge, UK- requires antigen retrieval protocol as previously described ( )).

    Techniques: shRNA, Expressing, Plasmid Preparation, Injection

    Molecular correlates of reoviral infection in cell preparations. Co-expression analysis of reovirus (red signal) and caspase-3 (brown signal) ( a ) in the cell line CAL27 shows that many of the cells with signal show apoptotic changes. The insert shows

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Reovirus-associated reduction of microRNA-let-7d is related to the increased apoptotic death of cancer cells in clinical samples

    doi: 10.1038/modpathol.2012.95

    Figure Lengend Snippet: Molecular correlates of reoviral infection in cell preparations. Co-expression analysis of reovirus (red signal) and caspase-3 (brown signal) ( a ) in the cell line CAL27 shows that many of the cells with signal show apoptotic changes. The insert shows

    Article Snippet: The following antibodies were used in this study (with optimal conditions and source in parentheses): tubulin (1:100, antigen retrieval, Abcam); caspase-3 (1:33, antigen retrieval, Abcam); PKR–phosphorylated form (1:50, antigen retrieval, Abcam); pERK (1:100, antigen retrieval, Abcam), Ras (1:10, antigen retrieval, Abcam), and p38 (1:250, antigen retrieval, Abcam).

    Techniques: Infection, Expressing

    Sequence homology between microRNA-let-7d and reovirus and luciferase assay for caspase-3 regulation by the microRNA. ( a ) Shows a representative analysis between the reovirus strain used in this study (serotype-3, Dearing strain) and microRNA-let-7d as

    Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc

    Article Title: Reovirus-associated reduction of microRNA-let-7d is related to the increased apoptotic death of cancer cells in clinical samples

    doi: 10.1038/modpathol.2012.95

    Figure Lengend Snippet: Sequence homology between microRNA-let-7d and reovirus and luciferase assay for caspase-3 regulation by the microRNA. ( a ) Shows a representative analysis between the reovirus strain used in this study (serotype-3, Dearing strain) and microRNA-let-7d as

    Article Snippet: The following antibodies were used in this study (with optimal conditions and source in parentheses): tubulin (1:100, antigen retrieval, Abcam); caspase-3 (1:33, antigen retrieval, Abcam); PKR–phosphorylated form (1:50, antigen retrieval, Abcam); pERK (1:100, antigen retrieval, Abcam), Ras (1:10, antigen retrieval, Abcam), and p38 (1:250, antigen retrieval, Abcam).

    Techniques: Sequencing, Luciferase

    Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Journal: Nature Communications

    Article Title: miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling

    doi: 10.1038/s41467-018-04831-3

    Figure Lengend Snippet: Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Article Snippet: In brief, tissue sections were incubated in Tris EDTA buffer at 95 °C to retrieve antigenicity, followed by incubation with the anti-CD11b primary antibody (ab133357, Abcam).

    Techniques: Mouse Assay

    Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Journal: Nature Communications

    Article Title: miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling

    doi: 10.1038/s41467-018-04831-3

    Figure Lengend Snippet: Long-term miR-143-145 deficiency results in myeloid expansion. a White blood cell (WBC), hemoglobin (HGB), and platelet (PLT) counts in peripheral blood of 80-week-old wild-type (WT; n = 28) and miR-143/145 −/− mice ( n = 23). b Micrographs of spleen sections from WT and miR-143/145 −/− mice immunostained for Mac1 (CD11b) (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). c Microscopic images of liver sections from WT and miR-143/145 −/− mice immunostained for CD11b (scale bar: 50 μm). Insets show higher magnification of selected area (scale bar: 100 μm). d – g Linear regression and Pearson's correlation analysis was performed on data from aged miR-143/145 −/− mice (82–101 weeks old) to compare the relationship between spleen/liver weights and WBC count ( d , e , n = 17), spleen and liver weight ( f , n = 17), and WBC count and the number of progenitors (Lin − Sca1 − c-Kit + ) ( g , n = 8)

    Article Snippet: In brief, tissue sections were incubated in Tris EDTA buffer at 95 °C to retrieve antigenicity, followed by incubation with the anti-CD11b primary antibody (ab133357, Abcam).

    Techniques: Mouse Assay