antibody occludin  (Abcam)

 
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    Name:
    Anti Occludin antibody
    Description:

    Catalog Number:
    ab31721
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    Structured Review

    Abcam antibody occludin
    In vitro mechanical injury induces the release of eMVs containing the endothelial marker PECAM-1 and tight junction protein <t>occludin.</t> (A) Injury increases the release of eMVs containing PECAM-1. eMVs were isolated from media using Exoquick and analyzed by western blot. Elevated levels of PECAM-1 were detected at 2 h and remained elevated for most time points. (Two-way ANOVA with Bonferroni post-hoc analysis ** p

    https://www.bioz.com/result/antibody occludin/product/Abcam
    Average 99 stars, based on 1 article reviews
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    Images

    1) Product Images from "Mechanical Injury Induces Brain Endothelial-Derived Microvesicle Release: Implications for Cerebral Vascular Injury during Traumatic Brain Injury"

    Article Title: Mechanical Injury Induces Brain Endothelial-Derived Microvesicle Release: Implications for Cerebral Vascular Injury during Traumatic Brain Injury

    Journal: Frontiers in Cellular Neuroscience

    doi: 10.3389/fncel.2016.00043

    In vitro mechanical injury induces the release of eMVs containing the endothelial marker PECAM-1 and tight junction protein occludin. (A) Injury increases the release of eMVs containing PECAM-1. eMVs were isolated from media using Exoquick and analyzed by western blot. Elevated levels of PECAM-1 were detected at 2 h and remained elevated for most time points. (Two-way ANOVA with Bonferroni post-hoc analysis ** p
    Figure Legend Snippet: In vitro mechanical injury induces the release of eMVs containing the endothelial marker PECAM-1 and tight junction protein occludin. (A) Injury increases the release of eMVs containing PECAM-1. eMVs were isolated from media using Exoquick and analyzed by western blot. Elevated levels of PECAM-1 were detected at 2 h and remained elevated for most time points. (Two-way ANOVA with Bonferroni post-hoc analysis ** p

    Techniques Used: In Vitro, Marker, Isolation, Western Blot

    Experimental TBI induces the release of eMVs containing the tight junction protein occludin . Mice were subjected to sham surgery or a Controlled Cortical Impact, CCI-TBI and blood was collected 24 h post-injury. (A) Flow Cytometry detection of occludin (FITC) positive eMVs from blood plasma. Regions of interest were drawn around all FITC positive vesicles as well as a clear high FSC population. (B,C) TBI induces an increase in total FITC positive and high FSC-FITC positive vesicles at 24 h post-injury (Students two-tailed t -test average ± SEM n = 5 for sham and n = 4 for TBI, * p
    Figure Legend Snippet: Experimental TBI induces the release of eMVs containing the tight junction protein occludin . Mice were subjected to sham surgery or a Controlled Cortical Impact, CCI-TBI and blood was collected 24 h post-injury. (A) Flow Cytometry detection of occludin (FITC) positive eMVs from blood plasma. Regions of interest were drawn around all FITC positive vesicles as well as a clear high FSC population. (B,C) TBI induces an increase in total FITC positive and high FSC-FITC positive vesicles at 24 h post-injury (Students two-tailed t -test average ± SEM n = 5 for sham and n = 4 for TBI, * p

    Techniques Used: Mouse Assay, Flow Cytometry, Cytometry, Two Tailed Test

    Related Articles

    Western Blot:

    Article Title: MST1 Suppression Reduces Early Brain Injury by Inhibiting the NF-κB/MMP-9 Pathway after Subarachnoid Hemorrhage in Mice
    Article Snippet: .. Western Blotting The ipsilateral/right cortex was isolated and homogenized for Western blotting as previously described [ ] with the following primary antibodies: anti-MST1 (1 : 1000, Cell Signaling Technology, Danvers, MA, USA), anti-phosphorylated MST1 (1 : 1000, Cell Signaling Technology, Danvers, MA, USA), anti-ZO-1 (1 : 500 Invitrogen, Grand Island, NY, USA), anti-occludin (1 : 1000 Abcam, Shanghai, China), anti-claudin-5 (1 : 1000, Abcam, Shanghai, China), anti-NF-κ B (1 : 1000, Cell Signaling Technology, Danvers, MA, USA), and anti-matrix metallopeptidase- (MMP-) 9 (1 : 1000, Cell Signaling Technology, Danvers, MA, USA). .. GAPDH (1 : 10,000, Proteintech, Rosemont, IL, USA) was used as an internal loading control.

    Incubation:

    Article Title: 1, 25-D3 Protects From Cerebral Ischemia by Maintaining BBB Permeability via PPAR-γ Activation
    Article Snippet: .. The slices were first incubated with the blocking solution (0.1% Triton X-100 in 0.1 M PBS, 10% bovine serum albumin, pH 7.6) for 2 h and then overnight with anti-occludin (1:100, Abcam, USA) and anti-claudin-5 (1:100, Thermo Fisher Scientific, Rockford, IL, USA) antibodies at 4°C. .. After washing with PBS, the tissues were incubated with the secondary antibodies and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime, China).

    Article Title: Comparison of Melatonin, Hypertonic Saline, and Hydroxyethyl Starch for Resuscitation of Secondary Intra-Abdominal Hypertension in an Animal Model
    Article Snippet: .. The membranes were incubated with anti-phospho-Akt (1:2000; Cell Signaling Technology, Danvers, MA, USA), anti-Akt (1:1000; Cell Signaling Technology, Danvers, MA, USA), anti- zonula occludens-1(ZO-1) (1:50; Abcam, Cambridge, MA, USA), and anti-occludin (1:100; Abcam, Cambridge, MA, USA) antibodies overnight at 4°C. .. The membrane-bound antibodies were visualized using IRDye 800 infrared-labeled donkey anti-rabbit secondary antibodies (1:15 000;Li-Cor Bioscience, Lincoln, NE,USA)and the Odyssey Infrared Imaging System (Li-Cor Bioscience, Bad Homburg, Germany).

    Article Title: Attaching-and-Effacing Pathogens Exploit Junction Regulatory Activities of N-WASP and SNX9 to Disrupt the Intestinal Barrier
    Article Snippet: .. After incubation with anti–E-cadherin (Cell Signaling Technology), anti-occludin (Abcam), or anti–zonula occludens-1 (ZO-1) (Abcam) primary antibodies, all at 1:400, slides were washed and incubated with Alexa 594–conjugated goat anti-rabbit or Alexa 488–conjugated goat anti-rabbit secondary antibodies and DAPI (Life Technologies, Grand Island, NY). .. Standard epifluorescence microscopy was performed using an AX70 upright microscope (Olympus, Tokyo, Japan).

    Article Title: A Role of Exopolysaccharide Produced by Streptococcus thermophilus in the Intestinal Inflammation and Mucosal Barrier in Caco-2 Monolayer and Dextran Sulphate Sodium-Induced Experimental Murine Colitis
    Article Snippet: .. Then the proteins were electro-transferred to PVDF member at 200 mA for 2 h. The membranes were incubated with primary antibodies: anti-β-actin (1:2500, Bioss, Beijing, China), Anti-Occludin (1:50,000, Abcam, Cambridge, MA, USA), anti-E-cadherin (1:1000, Abcam, USA), anti-Claudin1 (1:2000, Abcam, USA) for 12 h at 4 °C. .. After 5 times washed with 0.1 g/100 mL Tween-20 in PBS, then incubated with secondary antibodies (anti-rabbit and anti-mouse, 1:1000, Beyotime, Shanghai, China) for 1 h at room temperature.

    Article Title: Analysis of differential expression of tight junction proteins in cultured oral epithelial cells altered by Porphyromonas gingivalis, Porphyromonas gingivalis lipopolysaccharide, and extracellular adenosine triphosphate
    Article Snippet: .. The nitrocellulose membranes were then incubated with rabbit polyclonal antibodies to human occludin (ab31721), JAM-A (ab106114), claudin-1 (ab15098), claudin-4 (ab53156), ZO-1 (ab59720), claudin-15 (ab215354; all 1 μg·mL−1 , Abcam, Cambridge, UK), and β-actin (0.1 μg·mL−1 , GenTex, Zeeland, MI, USA) in 0.05% Tween20/TBS for 4 h. β-actin was used as a loading control. .. After the incubation, the membranes were washed three times and subsequently incubated with alkaline phosphatase (AP)-conjugated secondary antibody (goat-anti rabbit IgG, DAKO) diluted 1:1 500 in Tween20/TBS for 2 h. Bound antibodies were displayed with AP substrate (Bio-Rad) after the development of reactivity for proteins.

    Blocking Assay:

    Article Title: 1, 25-D3 Protects From Cerebral Ischemia by Maintaining BBB Permeability via PPAR-γ Activation
    Article Snippet: .. The slices were first incubated with the blocking solution (0.1% Triton X-100 in 0.1 M PBS, 10% bovine serum albumin, pH 7.6) for 2 h and then overnight with anti-occludin (1:100, Abcam, USA) and anti-claudin-5 (1:100, Thermo Fisher Scientific, Rockford, IL, USA) antibodies at 4°C. .. After washing with PBS, the tissues were incubated with the secondary antibodies and counterstained with 4′,6-diamidino-2-phenylindole (DAPI, Beyotime, China).

    Isolation:

    Article Title: MST1 Suppression Reduces Early Brain Injury by Inhibiting the NF-κB/MMP-9 Pathway after Subarachnoid Hemorrhage in Mice
    Article Snippet: .. Western Blotting The ipsilateral/right cortex was isolated and homogenized for Western blotting as previously described [ ] with the following primary antibodies: anti-MST1 (1 : 1000, Cell Signaling Technology, Danvers, MA, USA), anti-phosphorylated MST1 (1 : 1000, Cell Signaling Technology, Danvers, MA, USA), anti-ZO-1 (1 : 500 Invitrogen, Grand Island, NY, USA), anti-occludin (1 : 1000 Abcam, Shanghai, China), anti-claudin-5 (1 : 1000, Abcam, Shanghai, China), anti-NF-κ B (1 : 1000, Cell Signaling Technology, Danvers, MA, USA), and anti-matrix metallopeptidase- (MMP-) 9 (1 : 1000, Cell Signaling Technology, Danvers, MA, USA). .. GAPDH (1 : 10,000, Proteintech, Rosemont, IL, USA) was used as an internal loading control.

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  • 94
    Abcam occludin
    Tight Junction Protein, <t>Occludin,</t> Expression in the Cerebral Vasculature of C57BL/6 Mice is not Altered through Exposure to Mixed Vehicle Emissions Double immunofluorescence of occludin (red) and vWF (green) in cerebral microvessels from 4 mo old C57BL/6 mice on a low-fat (LF; A–C) or high-fat diet (HF; G–I) exposed filtered air (FA) or on a LF (D–F) or HF diet (J–L) and to exposed MVE at 100 µg/m 3 PM of mixed gasoline and diesel engine emissions for 6hr/d, 7 d/wk, for 30 d (n=3–4 per exposure group; 4 sections each, 3–5 vessels per section). Right panels (yellow; C, F, I, L) are merged figures of left (red; A, D, G, J) and center (green, E, H, K) panels for occludin and vWF. Blue fluorescence is Hoechst stained nuclei. Scale bar = 100 µm. Results shown are mean ± SEM.
    Occludin, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/occludin/product/Abcam
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    92
    Abcam rabbit anti occludin
    Adipose tissue extracts enable the EMT of CCA cells. Human cholangiocarcinoma (CCA) cell line RBE was incubated with complete growth medium or medium containing human adipose tissue extracts for 24 h. The cell morphology was observed under the invert microscopy (40×) ( a ). Protein expression of MMP2, MMP9, TGF-β1, claudin 1, <t>occludin,</t> E-cadherin, β-catenin, SNAIL, and Smad3 were analyzed by immunoblot analysis in response to adipose extracts incubation, and the band densitometry analysis was carried out relative to the loading control β-actin or GAPDH ( b , c ). To determine fatty acid metabolism, the culture medium with different adipose components prior to or after incubation with RBE cells for 24 h were collected for extracellular glycerol and nonestesterified fatty acid (NEFA) detection using colorimetric assays, with the data presented as the absorbance at 490 nm( d , e ). The intracellular lipids in adipose cultured RBE cells were visualized by the fluorescence dye Bodipy (20 μg/mL) using confocal microscopy analysis, using Hochest33342 (0.5 μg/mL) for nuclear staining ( f ). All the samples were prepared in triplicate, and all experiments were repeated at least three times. * P
    Rabbit Anti Occludin, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti occludin/product/Abcam
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    91
    Abcam mouse anti occludin
    TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker <t>occludin.</t> Representative images
    Mouse Anti Occludin, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 91 stars, based on 1 article reviews
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    Image Search Results


    Tight Junction Protein, Occludin, Expression in the Cerebral Vasculature of C57BL/6 Mice is not Altered through Exposure to Mixed Vehicle Emissions Double immunofluorescence of occludin (red) and vWF (green) in cerebral microvessels from 4 mo old C57BL/6 mice on a low-fat (LF; A–C) or high-fat diet (HF; G–I) exposed filtered air (FA) or on a LF (D–F) or HF diet (J–L) and to exposed MVE at 100 µg/m 3 PM of mixed gasoline and diesel engine emissions for 6hr/d, 7 d/wk, for 30 d (n=3–4 per exposure group; 4 sections each, 3–5 vessels per section). Right panels (yellow; C, F, I, L) are merged figures of left (red; A, D, G, J) and center (green, E, H, K) panels for occludin and vWF. Blue fluorescence is Hoechst stained nuclei. Scale bar = 100 µm. Results shown are mean ± SEM.

    Journal: Environmental research

    Article Title: Exposure to Traffic-Generated Air Pollutants Mediates Alterations in Brain Microvascular Integrity in Wildtype Mice on a High-Fat Diet

    doi: 10.1016/j.envres.2017.10.029

    Figure Lengend Snippet: Tight Junction Protein, Occludin, Expression in the Cerebral Vasculature of C57BL/6 Mice is not Altered through Exposure to Mixed Vehicle Emissions Double immunofluorescence of occludin (red) and vWF (green) in cerebral microvessels from 4 mo old C57BL/6 mice on a low-fat (LF; A–C) or high-fat diet (HF; G–I) exposed filtered air (FA) or on a LF (D–F) or HF diet (J–L) and to exposed MVE at 100 µg/m 3 PM of mixed gasoline and diesel engine emissions for 6hr/d, 7 d/wk, for 30 d (n=3–4 per exposure group; 4 sections each, 3–5 vessels per section). Right panels (yellow; C, F, I, L) are merged figures of left (red; A, D, G, J) and center (green, E, H, K) panels for occludin and vWF. Blue fluorescence is Hoechst stained nuclei. Scale bar = 100 µm. Results shown are mean ± SEM.

    Article Snippet: Frozen brain sections (10 µm) of the cerebrum (between Bregma 0 through −2.5mm) were prepared for either LOX-1, CD-36, 4-HNE, occludin, claudin-5, MMP-9, and/or double immunofluorescence, using techniques previously described by our laboratory ( ; ) with the following primary antibodies: MMP-9 (1:1000; Abcam, Cambridge, MA; Cat. #38898), occludin (1:500; Abcam #168986), claudin-5 (1:500; Abcam #15106), LOX-1 (1:1000; Abcam #60178), CD-36 (1:1000; Abcam #64014), 4-HNE (1:1000; Abcam #46545), AT-1 (1:250; Abcam #18801) and von Willebrand factor (vWF) (1:1000; Abcam #11713) and anti-rabbit Alexa Fluor 555 (1:500) and anti-sheep Alexa Fluor 455 (1:500) secondary antibodies.

    Techniques: Expressing, Mouse Assay, Immunofluorescence, Fluorescence, Staining

    Adipose tissue extracts enable the EMT of CCA cells. Human cholangiocarcinoma (CCA) cell line RBE was incubated with complete growth medium or medium containing human adipose tissue extracts for 24 h. The cell morphology was observed under the invert microscopy (40×) ( a ). Protein expression of MMP2, MMP9, TGF-β1, claudin 1, occludin, E-cadherin, β-catenin, SNAIL, and Smad3 were analyzed by immunoblot analysis in response to adipose extracts incubation, and the band densitometry analysis was carried out relative to the loading control β-actin or GAPDH ( b , c ). To determine fatty acid metabolism, the culture medium with different adipose components prior to or after incubation with RBE cells for 24 h were collected for extracellular glycerol and nonestesterified fatty acid (NEFA) detection using colorimetric assays, with the data presented as the absorbance at 490 nm( d , e ). The intracellular lipids in adipose cultured RBE cells were visualized by the fluorescence dye Bodipy (20 μg/mL) using confocal microscopy analysis, using Hochest33342 (0.5 μg/mL) for nuclear staining ( f ). All the samples were prepared in triplicate, and all experiments were repeated at least three times. * P

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Adipocytes promote cholangiocarcinoma metastasis through fatty acid binding protein 4

    doi: 10.1186/s13046-017-0641-y

    Figure Lengend Snippet: Adipose tissue extracts enable the EMT of CCA cells. Human cholangiocarcinoma (CCA) cell line RBE was incubated with complete growth medium or medium containing human adipose tissue extracts for 24 h. The cell morphology was observed under the invert microscopy (40×) ( a ). Protein expression of MMP2, MMP9, TGF-β1, claudin 1, occludin, E-cadherin, β-catenin, SNAIL, and Smad3 were analyzed by immunoblot analysis in response to adipose extracts incubation, and the band densitometry analysis was carried out relative to the loading control β-actin or GAPDH ( b , c ). To determine fatty acid metabolism, the culture medium with different adipose components prior to or after incubation with RBE cells for 24 h were collected for extracellular glycerol and nonestesterified fatty acid (NEFA) detection using colorimetric assays, with the data presented as the absorbance at 490 nm( d , e ). The intracellular lipids in adipose cultured RBE cells were visualized by the fluorescence dye Bodipy (20 μg/mL) using confocal microscopy analysis, using Hochest33342 (0.5 μg/mL) for nuclear staining ( f ). All the samples were prepared in triplicate, and all experiments were repeated at least three times. * P

    Article Snippet: The primary antibodies used were rabbit anti-FABP4 (EPR3579, Abcam, Cambridge, MA, USA, 1:1000), rabbit anti-claudin 1 (Abcam, Cambridge, MA, USA, 1:1000), rabbit anti-occludin (EPR8208, Abcam, Cambridge, MA, USA, 1:50,000), rabbit anti-E-cadherin (EP700Y, Abcam, Cambridge, MA, USA, 1:10,000), rabbit anti-SNAIL (Abcam, Cambridge, MA, USA, 1:1000), rabbit anti-Smad3 (EP568Y, Abcam, Cambridge, MA, USA, 1:1000), rabbit anti-β-catenin (Cell Signaling Technology, Danvers, MA, USA, 1:1000), rabbit anti-MMP2 (Cell Signaling Technology, Danvers, MA, USA,1:1000), rabbit anti-MMP9 (Cell Signaling Technology, Danvers, MA, USA,1:1000), and anti-β-actin (Beyotime Biotechnology, Haimen, China).

    Techniques: Incubation, Microscopy, Expressing, Cell Culture, Fluorescence, Confocal Microscopy, Staining

    Spastin OE does not impair tight junction localization or function. ( A ) ZO-1 localization in control and K10-rtTA; TRE-spastin epidermis. Scale-25µm. ( B ) Region where spastin-positive cells are next to spastin-negative cells in K10-rtTA; TRE-spastin tissue. Note that ZO-1 is still cortically localized in spastin-positive cells. Scale-10µm. ( C ) Localization of occludin at the cell cortex is maintained in K10-rtTA; TRE-spastin epidermis. Scale-10µm. ( D ) Biotin diffusion is blocked by occludin in K10-rtTA; TRE-spastin epidermis. Scale-10µm.

    Journal: eLife

    Article Title: A transgenic toolkit for visualizing and perturbing microtubules reveals unexpected functions in the epidermis

    doi: 10.7554/eLife.29834

    Figure Lengend Snippet: Spastin OE does not impair tight junction localization or function. ( A ) ZO-1 localization in control and K10-rtTA; TRE-spastin epidermis. Scale-25µm. ( B ) Region where spastin-positive cells are next to spastin-negative cells in K10-rtTA; TRE-spastin tissue. Note that ZO-1 is still cortically localized in spastin-positive cells. Scale-10µm. ( C ) Localization of occludin at the cell cortex is maintained in K10-rtTA; TRE-spastin epidermis. Scale-10µm. ( D ) Biotin diffusion is blocked by occludin in K10-rtTA; TRE-spastin epidermis. Scale-10µm.

    Article Snippet: The following primary antibodies were used in this study: rat anti-HA (11867423001, Sigma-Aldrich), rat anti-α-tubulin (sc-53029, Santa Cruz), rabbit anti-keratin 6 (PRB-169P, Covance), chicken anti-keratin 5/14 (generated in the Lechler lab), rabbit anti-keratin 10 (905401, Covance), rabbit anti-filaggrin (905801, Biolegend), rabbit anti-loricrin (kind gift from Colin Jamera), rat anti-BrdU (ab6326, Abcam), rabbit anti-active-caspase-3 (AF835, R and D systems), rat anti-β4 integrin (553745, BD Biosciences), rat anti-ECCD2 (kind gift from Colin Jamora), rabbit anti-keratin 1 (kind gift from Colin Jamora), mouse anti-desmoplakin (CBL173, Chemicon/Millipore), mouse anti-desmocollin-2/3 (clone 7G6, Santa Cruz), mouse anti-desmoglein-1 (610273, BD Biosciences), rabbit anti-phospho-myosin light chain 2 (Thr18/Ser19) (3674, Cell Signaling), rabbit anti-occludin (ab3172, Abcam), rabbit anti-ZO-1 (61–7300, Zymed/Invitrogen), rabbit anti-centrin1 (ab101332, Abcam), mouse anti-acetylated tubulin (T7451, Sigma-Aldrich), Rhodamine Ulex Europaeus Agglutinin 1 (RL-1062, Vector Laboratories).

    Techniques: Diffusion-based Assay

    TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker occludin. Representative images

    Journal: The Journal of Biological Chemistry

    Article Title: Endothelial Surface N-Glycans Mediate Monocyte Adhesion and Are Targets for Anti-inflammatory Effects of Peroxisome Proliferator-activated Receptor ? Ligands *

    doi: 10.1074/jbc.M111.247981

    Figure Lengend Snippet: TNFα enriches N -glycans at cell junctions. Panel A , HUVECs were treated with TNFα (10 ng/ml, 4 h) and N -glycans were stained using the indicated lectins. Cells were then stained for the tight junction marker occludin. Representative images

    Article Snippet: After blocking, coverslips were incubated with mouse anti-occludin (1:200, Abcam) in PBS containing 5% goat serum overnight at 4 °C.

    Techniques: Staining, Marker