Journal: Neural Regeneration Research

Article Title: Recombinant chitinase-3-like protein 1 alleviates learning and memory impairments via M2 microglia polarization in postoperative cognitive dysfunction mice

doi: 10.4103/NRR.NRR-D-23-01233

Figure Lengend Snippet: Representative western blots and densitometric analysis showing the protein expression of iNOS, IL-1β, CD206, Arg-1 and pERK in the hippocampus on days 3 and 7 after surgery and CHI3L1 treatment. * P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc test). The experiments were repeated three times. Arg-1: Arginase-1; C: control; CHI3L1: chitinase-3–like protein 1; IL-1β: interleukin-1β; iNOS: inducible nitric oxide synthase; pERK: phosphorylated extracellular signal-regulated kinase; rCLP: recombinant CHI3L1; S: surgery; Veh: vehicle.

Article Snippet: The blots were blocked with 5% bovine serum albumin for 2 hours and incubated with primary antibodies against the following proteins: mouse anti-tubulin (1:7500, AT8191, Beyotime); rabbit anti-CHI3L1 (1:1000, 12036-1-AP), rabbit anti-CD206 (1:1000, 12036-1-AP), rabbit anti-interleukin-13 receptor subunit α2 (IL13Rα2; 1:1000, 11059-1-AP) and rabbit anti-Arg1 (1:1000, 16001-1-AP) (all from Proteintech, Wuhan, China); rabbit anti-phosphoERK1/2 (Thr202/Tyr204; #4370, 1:1000) and rabbit anti-inducible nitric oxide synthase (iNOS; 1:1000, 13120S) (all from Cell Signaling Technology, Danvers, MA, USA); rabbit anti-interleukin-1 beta (IL-1β; 1:1000, ab9722), rabbit anti-brain-derived neurotrophic factor (BDNF; 1:3000, ab108319), and rabbit anti-phosphorylated N-methyl-D-aspartic acid receptor 2B subunit (p-NR2B; 1:1000, ab3856) (all from Abcam, Cambridge, MA, USA) at 4°C overnight.

Techniques: Western Blot, Expressing, Control, Recombinant

Journal: Neural Regeneration Research

Article Title: Recombinant chitinase-3-like protein 1 alleviates learning and memory impairments via M2 microglia polarization in postoperative cognitive dysfunction mice

doi: 10.4103/NRR.NRR-D-23-01233

Figure Lengend Snippet: Changes in hippocampal iNOS, IL-1β, and CD206 protein expression and behavioral tests after ERK inhibitor treatment. (A) Representative western blots and densitometric analysis showing the expression of iNOS, IL-1β, and CD206 in the hippocampus on day 3 after surgery and treatment. (B) Spontaneous alternation (%) and (C) freezing time (%) of each group on day 3 after surgery. (D) Representative western blots and densitometric analysis showing the expression of BDNF and pNR2B in the hippocampus on day 3 after surgery. The experiments were repeated three times. * P < 0.05 (one-way analysis of variance followed by Tukey’s post hoc test). CHI3L1: Chitinase-3–like protein 1; ERK: extracellular signal–regulated kinase; IL-1β: interleukin-1β; iNOS: inducible nitric oxide synthase; PD: ERK inhibitor PD98059; rCLP: recombinant CHI3L1; S: surgery; Veh: vehicle.

Article Snippet: The blots were blocked with 5% bovine serum albumin for 2 hours and incubated with primary antibodies against the following proteins: mouse anti-tubulin (1:7500, AT8191, Beyotime); rabbit anti-CHI3L1 (1:1000, 12036-1-AP), rabbit anti-CD206 (1:1000, 12036-1-AP), rabbit anti-interleukin-13 receptor subunit α2 (IL13Rα2; 1:1000, 11059-1-AP) and rabbit anti-Arg1 (1:1000, 16001-1-AP) (all from Proteintech, Wuhan, China); rabbit anti-phosphoERK1/2 (Thr202/Tyr204; #4370, 1:1000) and rabbit anti-inducible nitric oxide synthase (iNOS; 1:1000, 13120S) (all from Cell Signaling Technology, Danvers, MA, USA); rabbit anti-interleukin-1 beta (IL-1β; 1:1000, ab9722), rabbit anti-brain-derived neurotrophic factor (BDNF; 1:3000, ab108319), and rabbit anti-phosphorylated N-methyl-D-aspartic acid receptor 2B subunit (p-NR2B; 1:1000, ab3856) (all from Abcam, Cambridge, MA, USA) at 4°C overnight.

Techniques: Expressing, Western Blot, Recombinant

Journal: Poultry Science

Article Title: Preclinical evaluation of a live avian metapneumovirus subtype B vaccine strain with cross-protective efficacy in chickens

doi: 10.1016/j.psj.2025.105283

Figure Lengend Snippet: Correlation between vaccine antibody titers before challenge and viral shedding after challenge. Serological and virological data obtained from chickens ( n = 160) used in this study were used. Scatter plot showing the relationship between the viral load (Ct values) and ELISA antibody titers. P/N threshold, positive/negative threshold; H/L threshold, high/low viral load threshold.

Article Snippet: Serum samples were tested for the presence of aMPV antibodies using a quantitative commercial ELISA kit (FlockChek Avian Pneumovirus Antibody, IDEXX Laboratories, Westbrook, ME, USA) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay