Structured Review

Cell Signaling Technology Inc antibody expression
Antibody Expression, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/antibody expression/product/Cell Signaling Technology Inc
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
antibody expression - by Bioz Stars, 2020-09
90/100 stars

Images

Related Articles

Immunohistochemistry:

Article Title: Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential
Article Snippet: .. We thank Dr. Robert Schoen, the PI on the MUC1 vaccine trial, P. Beatty for advice on IHC protocols and Dr. J. McKolanis for advice on ELISA (University of Pittsburgh), C. Manning and M. Nelson for assistance with IF and internalization assays and image capture, K. Crosby and C Grange for preliminary IHC assays and intellectual discussion, R. Ramenani for NGS of the IgG repertoire, A. Moritz for MS processing of affinity-purified antibodies, T. Gu for intellectual discussion, D. Moore-Lai and T. Palazzola for antibody expression (Cell Signaling Technology). ..

Expressing:

Article Title: Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential
Article Snippet: .. We thank Dr. Robert Schoen, the PI on the MUC1 vaccine trial, P. Beatty for advice on IHC protocols and Dr. J. McKolanis for advice on ELISA (University of Pittsburgh), C. Manning and M. Nelson for assistance with IF and internalization assays and image capture, K. Crosby and C Grange for preliminary IHC assays and intellectual discussion, R. Ramenani for NGS of the IgG repertoire, A. Moritz for MS processing of affinity-purified antibodies, T. Gu for intellectual discussion, D. Moore-Lai and T. Palazzola for antibody expression (Cell Signaling Technology). ..

Next-Generation Sequencing:

Article Title: Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential
Article Snippet: .. We thank Dr. Robert Schoen, the PI on the MUC1 vaccine trial, P. Beatty for advice on IHC protocols and Dr. J. McKolanis for advice on ELISA (University of Pittsburgh), C. Manning and M. Nelson for assistance with IF and internalization assays and image capture, K. Crosby and C Grange for preliminary IHC assays and intellectual discussion, R. Ramenani for NGS of the IgG repertoire, A. Moritz for MS processing of affinity-purified antibodies, T. Gu for intellectual discussion, D. Moore-Lai and T. Palazzola for antibody expression (Cell Signaling Technology). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential
Article Snippet: .. We thank Dr. Robert Schoen, the PI on the MUC1 vaccine trial, P. Beatty for advice on IHC protocols and Dr. J. McKolanis for advice on ELISA (University of Pittsburgh), C. Manning and M. Nelson for assistance with IF and internalization assays and image capture, K. Crosby and C Grange for preliminary IHC assays and intellectual discussion, R. Ramenani for NGS of the IgG repertoire, A. Moritz for MS processing of affinity-purified antibodies, T. Gu for intellectual discussion, D. Moore-Lai and T. Palazzola for antibody expression (Cell Signaling Technology). ..

Mass Spectrometry:

Article Title: Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential
Article Snippet: .. We thank Dr. Robert Schoen, the PI on the MUC1 vaccine trial, P. Beatty for advice on IHC protocols and Dr. J. McKolanis for advice on ELISA (University of Pittsburgh), C. Manning and M. Nelson for assistance with IF and internalization assays and image capture, K. Crosby and C Grange for preliminary IHC assays and intellectual discussion, R. Ramenani for NGS of the IgG repertoire, A. Moritz for MS processing of affinity-purified antibodies, T. Gu for intellectual discussion, D. Moore-Lai and T. Palazzola for antibody expression (Cell Signaling Technology). ..

Affinity Purification:

Article Title: Antibodies elicited by the first non-viral prophylactic cancer vaccine show tumor-specificity and immunotherapeutic potential
Article Snippet: .. We thank Dr. Robert Schoen, the PI on the MUC1 vaccine trial, P. Beatty for advice on IHC protocols and Dr. J. McKolanis for advice on ELISA (University of Pittsburgh), C. Manning and M. Nelson for assistance with IF and internalization assays and image capture, K. Crosby and C Grange for preliminary IHC assays and intellectual discussion, R. Ramenani for NGS of the IgG repertoire, A. Moritz for MS processing of affinity-purified antibodies, T. Gu for intellectual discussion, D. Moore-Lai and T. Palazzola for antibody expression (Cell Signaling Technology). ..

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Cell Signaling Technology Inc lef 1 expression
    Two promoters in the LEF1 locus. The LEF1 locus contains two RNA polymerase II promoters. One promoter (P1) produces an mRNA that encodes full-length <t>LEF-1</t> protein. A second promoter in intron 2 (P2) produces an mRNA that encodes the shorter, dominant negative LEF-1 isoform. Both promoters contain bona fide WREs (blue WREs) that mediate activation by TCF–β-catenin complexes. P1 and P2 expression patterns are aberrant in colon cancer cells. P1 is aberrantly activated by TCF–β-catenin, whereas P2 is silenced by a distal repressor located between –1446 and –1281 nt upstream relative to the P2 transcription start site (red circle). The repressor targets a unique feature of basal P2 as P1 is not silenced by the repressor.
    Lef 1 Expression, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lef 1 expression/product/Cell Signaling Technology Inc
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lef 1 expression - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    85
    Cell Signaling Technology Inc ac3 expression
    Ketamine induces activated caspase-3 <t>(AC3):</t> Western blot analysis P7 rat pups were injected with vehicle (Veh; n = 5), MK801 (MK; 1 mg/kg; n = 5) or various doses of ketamine (K2, 2mg/kg; K5, 5 mg/kg; K10, 10 mg/kg or K20, 20 mg/kg; n = 6 for all doses). See Experimental Procedures for details. At 8 hr, proteins were extracted from the anterior somatosensory cortex and processed for expression of the 17 kDa band for the cleaved (activated) form of caspase-3 (AC3) A. Representative Western blot for Veh, MK801, or ketamine (K20)-treated rats. B. Mean band density (± SE) for AC3 (relative to β-actin); ***p
    Ac3 Expression, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ac3 expression/product/Cell Signaling Technology Inc
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ac3 expression - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    92
    Cell Signaling Technology Inc cofilin expression
    <t>Cofilin</t> regulates transient stretch-induced fluidization. A : normalized net contractile moment after a single transient isotropic stretch in control ( n = 27) and cofilin-knockdown ( n = 25) single isolated HASM cells. Inset : median of unnormalized contractile moment. Traction reduction is significantly less in cofilin-knockdown than control cells at 10, 70, and 130 s. Values are means ± SE.* P
    Cofilin Expression, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cofilin expression/product/Cell Signaling Technology Inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cofilin expression - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    85
    Cell Signaling Technology Inc stβrii expression
    Effects of HER2 silencing, lapatinib, and src inhibition on <t>sTβRII-mediated</t> reversal of growth suppression. A. HER2 silencing and inhibition. T3M4 cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), shHER2-LV (shHER2), or both shEGFR and shHER2, in the absence or presence of WPT-sTβRII (sTβRII) or 1 µM lapatinip. Colony formation was monitored in 3-D culture. Data are the means ± SEM of triplicate determinations from three independent experiments. *p
    Stβrii Expression, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stβrii expression/product/Cell Signaling Technology Inc
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    stβrii expression - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    Two promoters in the LEF1 locus. The LEF1 locus contains two RNA polymerase II promoters. One promoter (P1) produces an mRNA that encodes full-length LEF-1 protein. A second promoter in intron 2 (P2) produces an mRNA that encodes the shorter, dominant negative LEF-1 isoform. Both promoters contain bona fide WREs (blue WREs) that mediate activation by TCF–β-catenin complexes. P1 and P2 expression patterns are aberrant in colon cancer cells. P1 is aberrantly activated by TCF–β-catenin, whereas P2 is silenced by a distal repressor located between –1446 and –1281 nt upstream relative to the P2 transcription start site (red circle). The repressor targets a unique feature of basal P2 as P1 is not silenced by the repressor.

    Journal: Nucleic Acids Research

    Article Title: A role for YY1 in repression of dominant negative LEF-1 expression in colon cancer

    doi: 10.1093/nar/gkq492

    Figure Lengend Snippet: Two promoters in the LEF1 locus. The LEF1 locus contains two RNA polymerase II promoters. One promoter (P1) produces an mRNA that encodes full-length LEF-1 protein. A second promoter in intron 2 (P2) produces an mRNA that encodes the shorter, dominant negative LEF-1 isoform. Both promoters contain bona fide WREs (blue WREs) that mediate activation by TCF–β-catenin complexes. P1 and P2 expression patterns are aberrant in colon cancer cells. P1 is aberrantly activated by TCF–β-catenin, whereas P2 is silenced by a distal repressor located between –1446 and –1281 nt upstream relative to the P2 transcription start site (red circle). The repressor targets a unique feature of basal P2 as P1 is not silenced by the repressor.

    Article Snippet: Anti-flag (F-3165 Sigma) and anti-LEF1 (#2286 Cell Signaling) antibodies were used to detect LEF-1 expression and anti-Lamin A/C (#2032 Cell Signaling) was used as loading control.

    Techniques: Dominant Negative Mutation, Activation Assay, Expressing

    Overexpression of dnLEF-1 slows down DLD-1 colon cancer cell growth. The effect of doxycycline-induced expression of dnLEF-1 on DLD-1 colon cancer cell growth was monitored over a 10-day period in two different clonal stable cells lines [dnLEF1( 1 ) and dnLEF1( 2 )]. Quantitation of cell number with or without doxycycline (0.01 µg/ml) was performed using a sulforhodamine B cell proliferation assay (colorimetric-based growth curve). Differences in the rate of cell growth appeared after 4 days after which the dnLEF-1 expressing cells grew at a significantly reduced rate. A representative graph is shown from two independent trials. Error bars depict SDs of the results obtained with eight replicates from one trial. The western blots above the cell growth assay show the induced levels of expression of flag-tagged dnLEF-1 protein after 24 h of doxycycline treatment. Endogenous LEF-1 is also detected with LEF1 antibody (FL-LEF-1). Lamin antisera is used for a loading control.

    Journal: Nucleic Acids Research

    Article Title: A role for YY1 in repression of dominant negative LEF-1 expression in colon cancer

    doi: 10.1093/nar/gkq492

    Figure Lengend Snippet: Overexpression of dnLEF-1 slows down DLD-1 colon cancer cell growth. The effect of doxycycline-induced expression of dnLEF-1 on DLD-1 colon cancer cell growth was monitored over a 10-day period in two different clonal stable cells lines [dnLEF1( 1 ) and dnLEF1( 2 )]. Quantitation of cell number with or without doxycycline (0.01 µg/ml) was performed using a sulforhodamine B cell proliferation assay (colorimetric-based growth curve). Differences in the rate of cell growth appeared after 4 days after which the dnLEF-1 expressing cells grew at a significantly reduced rate. A representative graph is shown from two independent trials. Error bars depict SDs of the results obtained with eight replicates from one trial. The western blots above the cell growth assay show the induced levels of expression of flag-tagged dnLEF-1 protein after 24 h of doxycycline treatment. Endogenous LEF-1 is also detected with LEF1 antibody (FL-LEF-1). Lamin antisera is used for a loading control.

    Article Snippet: Anti-flag (F-3165 Sigma) and anti-LEF1 (#2286 Cell Signaling) antibodies were used to detect LEF-1 expression and anti-Lamin A/C (#2032 Cell Signaling) was used as loading control.

    Techniques: Over Expression, Expressing, Quantitation Assay, Proliferation Assay, Western Blot, Growth Assay

    Ketamine induces activated caspase-3 (AC3): Western blot analysis P7 rat pups were injected with vehicle (Veh; n = 5), MK801 (MK; 1 mg/kg; n = 5) or various doses of ketamine (K2, 2mg/kg; K5, 5 mg/kg; K10, 10 mg/kg or K20, 20 mg/kg; n = 6 for all doses). See Experimental Procedures for details. At 8 hr, proteins were extracted from the anterior somatosensory cortex and processed for expression of the 17 kDa band for the cleaved (activated) form of caspase-3 (AC3) A. Representative Western blot for Veh, MK801, or ketamine (K20)-treated rats. B. Mean band density (± SE) for AC3 (relative to β-actin); ***p

    Journal: Neuroscience

    Article Title: Is age-dependent, ketamine-induced apoptosis in the rat somatosensory cortex influenced by temperature?

    doi: 10.1016/j.neuroscience.2010.03.016

    Figure Lengend Snippet: Ketamine induces activated caspase-3 (AC3): Western blot analysis P7 rat pups were injected with vehicle (Veh; n = 5), MK801 (MK; 1 mg/kg; n = 5) or various doses of ketamine (K2, 2mg/kg; K5, 5 mg/kg; K10, 10 mg/kg or K20, 20 mg/kg; n = 6 for all doses). See Experimental Procedures for details. At 8 hr, proteins were extracted from the anterior somatosensory cortex and processed for expression of the 17 kDa band for the cleaved (activated) form of caspase-3 (AC3) A. Representative Western blot for Veh, MK801, or ketamine (K20)-treated rats. B. Mean band density (± SE) for AC3 (relative to β-actin); ***p

    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (Trans Blot Transfer Medium, BioRad, Hercules, CA), which were then probed for AC3 expression (rabbit anti-AC3, 1:1000, Cell Signaling; donkey HRP-conjugated anti-rabbit secondary, 1:5000, GE Healthcare, UK).

    Techniques: Western Blot, Injection, Expressing

    Ketamine induces changes in core body temperature (CBT) and activated caspase-3 (AC3) expression in P7 neonates A. CBT (mean ± SE) was measured at various times (0-8 hours) following injection of vehicle (V; n = 7), MK801 (1 mg/kg; n = 4) or ketamine (2-20 mg/kg; K2-K20; n = 4-5). B. Quantitative analysis of AC3 positive cells (mean ± SE) in layers IV and V of the anterior somatosensory cortex across all treatments (see Experimental Procedures). In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; ***p

    Journal: Neuroscience

    Article Title: Is age-dependent, ketamine-induced apoptosis in the rat somatosensory cortex influenced by temperature?

    doi: 10.1016/j.neuroscience.2010.03.016

    Figure Lengend Snippet: Ketamine induces changes in core body temperature (CBT) and activated caspase-3 (AC3) expression in P7 neonates A. CBT (mean ± SE) was measured at various times (0-8 hours) following injection of vehicle (V; n = 7), MK801 (1 mg/kg; n = 4) or ketamine (2-20 mg/kg; K2-K20; n = 4-5). B. Quantitative analysis of AC3 positive cells (mean ± SE) in layers IV and V of the anterior somatosensory cortex across all treatments (see Experimental Procedures). In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; ***p

    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (Trans Blot Transfer Medium, BioRad, Hercules, CA), which were then probed for AC3 expression (rabbit anti-AC3, 1:1000, Cell Signaling; donkey HRP-conjugated anti-rabbit secondary, 1:5000, GE Healthcare, UK).

    Techniques: Expressing, Injection

    Elevation of CBT does not alter ketamine-induced apoptosis in layers IV and V of the somatosensory cortex in P7 rats Animals were injected with vehicle or 40 mg/kg ketamine (K40) as described (see Experimental Procedures). For some K40 animals, CBT was not artificially maintained (K40-HT) whereas for other K40 animals CBT was artificially maintained (K40-NT; see Experimental Procedures) A. Changes in CBT (mean ± SE) after injection of vehicle (V; n = 7) or 40 mg/kg ketamine in the absence of presence of an artificial heat source (K40-HT, n = 4 or K40-NT, n = 4). B. Quantitative analysis of AC3 positive cells within layers IV/V of the anterior somatosensory cortex (see Experimental Procedures). In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; *p

    Journal: Neuroscience

    Article Title: Is age-dependent, ketamine-induced apoptosis in the rat somatosensory cortex influenced by temperature?

    doi: 10.1016/j.neuroscience.2010.03.016

    Figure Lengend Snippet: Elevation of CBT does not alter ketamine-induced apoptosis in layers IV and V of the somatosensory cortex in P7 rats Animals were injected with vehicle or 40 mg/kg ketamine (K40) as described (see Experimental Procedures). For some K40 animals, CBT was not artificially maintained (K40-HT) whereas for other K40 animals CBT was artificially maintained (K40-NT; see Experimental Procedures) A. Changes in CBT (mean ± SE) after injection of vehicle (V; n = 7) or 40 mg/kg ketamine in the absence of presence of an artificial heat source (K40-HT, n = 4 or K40-NT, n = 4). B. Quantitative analysis of AC3 positive cells within layers IV/V of the anterior somatosensory cortex (see Experimental Procedures). In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; *p

    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (Trans Blot Transfer Medium, BioRad, Hercules, CA), which were then probed for AC3 expression (rabbit anti-AC3, 1:1000, Cell Signaling; donkey HRP-conjugated anti-rabbit secondary, 1:5000, GE Healthcare, UK).

    Techniques: Injection

    Ketamine-induced changes in CBT are age-dependent A. CBT changes (mean ± SE) were measured in P14 and P21 animals after injection of vehicle (V; n = 2 both ages) or ketamine (K40; n = 3 both ages). B Quantitative analysis of AC3 positive cells within layers IV/V of the anterior somatosensory cortex (see Experimental Procedures) in P14 and P21 animals. In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; *p

    Journal: Neuroscience

    Article Title: Is age-dependent, ketamine-induced apoptosis in the rat somatosensory cortex influenced by temperature?

    doi: 10.1016/j.neuroscience.2010.03.016

    Figure Lengend Snippet: Ketamine-induced changes in CBT are age-dependent A. CBT changes (mean ± SE) were measured in P14 and P21 animals after injection of vehicle (V; n = 2 both ages) or ketamine (K40; n = 3 both ages). B Quantitative analysis of AC3 positive cells within layers IV/V of the anterior somatosensory cortex (see Experimental Procedures) in P14 and P21 animals. In A and B, differences in the means determined by ANOVA using a Bonferroni post-test comparison; *p

    Article Snippet: Separated proteins were transferred to nitrocellulose membranes (Trans Blot Transfer Medium, BioRad, Hercules, CA), which were then probed for AC3 expression (rabbit anti-AC3, 1:1000, Cell Signaling; donkey HRP-conjugated anti-rabbit secondary, 1:5000, GE Healthcare, UK).

    Techniques: Injection

    Cofilin regulates transient stretch-induced fluidization. A : normalized net contractile moment after a single transient isotropic stretch in control ( n = 27) and cofilin-knockdown ( n = 25) single isolated HASM cells. Inset : median of unnormalized contractile moment. Traction reduction is significantly less in cofilin-knockdown than control cells at 10, 70, and 130 s. Values are means ± SE.* P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Biomarkers in Lung Diseases: From Pathogenesis to Prediction to New Therapies: Transient stretch induces cytoskeletal fluidization through the severing action of cofilin

    doi: 10.1152/ajplung.00326.2017

    Figure Lengend Snippet: Cofilin regulates transient stretch-induced fluidization. A : normalized net contractile moment after a single transient isotropic stretch in control ( n = 27) and cofilin-knockdown ( n = 25) single isolated HASM cells. Inset : median of unnormalized contractile moment. Traction reduction is significantly less in cofilin-knockdown than control cells at 10, 70, and 130 s. Values are means ± SE.* P

    Article Snippet: Because the antibody we used to probe cofilin expression is cofilin-1-specific (catalog no. 5175, Cell Signaling Technology), the targeting antigen of this antibody shares little homology with similar proteins, such as ADF and cofilin-2.

    Techniques: Isolation

    Cofilin knockdown reduces cytoskeletal remodeling in HASM cells. Mean square displacement (MSD) of microbeads adherent to cytoskeleton from control and cofilin-knockdown cells; cofilin knockdown leads to a slower rate of remodeling, as evidenced by a downward shift of the MSD curve. Dashed lines indicate diffusion exponents: diffusive ( slope 1 ) and ballistic ( slope 2 ).

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Biomarkers in Lung Diseases: From Pathogenesis to Prediction to New Therapies: Transient stretch induces cytoskeletal fluidization through the severing action of cofilin

    doi: 10.1152/ajplung.00326.2017

    Figure Lengend Snippet: Cofilin knockdown reduces cytoskeletal remodeling in HASM cells. Mean square displacement (MSD) of microbeads adherent to cytoskeleton from control and cofilin-knockdown cells; cofilin knockdown leads to a slower rate of remodeling, as evidenced by a downward shift of the MSD curve. Dashed lines indicate diffusion exponents: diffusive ( slope 1 ) and ballistic ( slope 2 ).

    Article Snippet: Because the antibody we used to probe cofilin expression is cofilin-1-specific (catalog no. 5175, Cell Signaling Technology), the targeting antigen of this antibody shares little homology with similar proteins, such as ADF and cofilin-2.

    Techniques: Diffusion-based Assay

    Cofilin regulates poststretch actin filament disassembly. A : normalized changes in actin fluorescence intensity after a single transient isotropic stretch in control and cofilin-knockdown HASM cells. Actin intensity is significantly higher in cofilin-knockdown than control cells at 10 and 200 s after a transient stretch. Values are means ± SD; n = 9. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Biomarkers in Lung Diseases: From Pathogenesis to Prediction to New Therapies: Transient stretch induces cytoskeletal fluidization through the severing action of cofilin

    doi: 10.1152/ajplung.00326.2017

    Figure Lengend Snippet: Cofilin regulates poststretch actin filament disassembly. A : normalized changes in actin fluorescence intensity after a single transient isotropic stretch in control and cofilin-knockdown HASM cells. Actin intensity is significantly higher in cofilin-knockdown than control cells at 10 and 200 s after a transient stretch. Values are means ± SD; n = 9. * P

    Article Snippet: Because the antibody we used to probe cofilin expression is cofilin-1-specific (catalog no. 5175, Cell Signaling Technology), the targeting antigen of this antibody shares little homology with similar proteins, such as ADF and cofilin-2.

    Techniques: Fluorescence

    Cofilin knockdown enhances traction force and filamentous actin (F-actin) in HASM cells. A : corresponding traction force is significantly greater in cofilin-knockdown cells. Values are means ± SD; n = 33 per group. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Biomarkers in Lung Diseases: From Pathogenesis to Prediction to New Therapies: Transient stretch induces cytoskeletal fluidization through the severing action of cofilin

    doi: 10.1152/ajplung.00326.2017

    Figure Lengend Snippet: Cofilin knockdown enhances traction force and filamentous actin (F-actin) in HASM cells. A : corresponding traction force is significantly greater in cofilin-knockdown cells. Values are means ± SD; n = 33 per group. * P

    Article Snippet: Because the antibody we used to probe cofilin expression is cofilin-1-specific (catalog no. 5175, Cell Signaling Technology), the targeting antigen of this antibody shares little homology with similar proteins, such as ADF and cofilin-2.

    Techniques:

    Cofilin dephosphorylates immediately after a transient stretch. Top : level of phosphorylated cofilin (p-Cof) decreases significantly only after a transient stretch. Values are means ± SD; n = 4. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Biomarkers in Lung Diseases: From Pathogenesis to Prediction to New Therapies: Transient stretch induces cytoskeletal fluidization through the severing action of cofilin

    doi: 10.1152/ajplung.00326.2017

    Figure Lengend Snippet: Cofilin dephosphorylates immediately after a transient stretch. Top : level of phosphorylated cofilin (p-Cof) decreases significantly only after a transient stretch. Values are means ± SD; n = 4. * P

    Article Snippet: Because the antibody we used to probe cofilin expression is cofilin-1-specific (catalog no. 5175, Cell Signaling Technology), the targeting antigen of this antibody shares little homology with similar proteins, such as ADF and cofilin-2.

    Techniques:

    Cofilin knockdown in human airway smooth muscle (HASM) cells. Quantification ( top ) of a representative Western blot ( bottom ) showing substantially reduced protein expression levels of cofilin (Cof) at 72 h after siRNA transfection. Values are means ± SD; n = 5. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Biomarkers in Lung Diseases: From Pathogenesis to Prediction to New Therapies: Transient stretch induces cytoskeletal fluidization through the severing action of cofilin

    doi: 10.1152/ajplung.00326.2017

    Figure Lengend Snippet: Cofilin knockdown in human airway smooth muscle (HASM) cells. Quantification ( top ) of a representative Western blot ( bottom ) showing substantially reduced protein expression levels of cofilin (Cof) at 72 h after siRNA transfection. Values are means ± SD; n = 5. * P

    Article Snippet: Because the antibody we used to probe cofilin expression is cofilin-1-specific (catalog no. 5175, Cell Signaling Technology), the targeting antigen of this antibody shares little homology with similar proteins, such as ADF and cofilin-2.

    Techniques: Western Blot, Expressing, Transfection

    Except in the case of cofilin knockdown, the fluidization response is roughly a constant fraction of baseline traction. A : at baseline, traction exerted by pharmacologically treated [ML-7, histamine, FBS, N -acylethanolamide phosphate (NAEPA), and cofilin knockdown (KD)] HASM cells is substantially different from that exerted by control cells. Values are means ± SE; n = 7 per group. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Biomarkers in Lung Diseases: From Pathogenesis to Prediction to New Therapies: Transient stretch induces cytoskeletal fluidization through the severing action of cofilin

    doi: 10.1152/ajplung.00326.2017

    Figure Lengend Snippet: Except in the case of cofilin knockdown, the fluidization response is roughly a constant fraction of baseline traction. A : at baseline, traction exerted by pharmacologically treated [ML-7, histamine, FBS, N -acylethanolamide phosphate (NAEPA), and cofilin knockdown (KD)] HASM cells is substantially different from that exerted by control cells. Values are means ± SE; n = 7 per group. * P

    Article Snippet: Because the antibody we used to probe cofilin expression is cofilin-1-specific (catalog no. 5175, Cell Signaling Technology), the targeting antigen of this antibody shares little homology with similar proteins, such as ADF and cofilin-2.

    Techniques:

    Effects of HER2 silencing, lapatinib, and src inhibition on sTβRII-mediated reversal of growth suppression. A. HER2 silencing and inhibition. T3M4 cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), shHER2-LV (shHER2), or both shEGFR and shHER2, in the absence or presence of WPT-sTβRII (sTβRII) or 1 µM lapatinip. Colony formation was monitored in 3-D culture. Data are the means ± SEM of triplicate determinations from three independent experiments. *p

    Journal: PLoS ONE

    Article Title: Concomitant Targeting of EGF Receptor, TGF-beta and Src Points to a Novel Therapeutic Approach in Pancreatic Cancer

    doi: 10.1371/journal.pone.0039684

    Figure Lengend Snippet: Effects of HER2 silencing, lapatinib, and src inhibition on sTβRII-mediated reversal of growth suppression. A. HER2 silencing and inhibition. T3M4 cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), shHER2-LV (shHER2), or both shEGFR and shHER2, in the absence or presence of WPT-sTβRII (sTβRII) or 1 µM lapatinip. Colony formation was monitored in 3-D culture. Data are the means ± SEM of triplicate determinations from three independent experiments. *p

    Article Snippet: Authenticity was confirmed by sequencing, and sTβRII expression was assessed by immunoblotting for HA (Cell Signaling, Danvers, MA).

    Techniques: Inhibition, Infection

    Effects of targeting EGFR and TGF-β pathways on phosphorylation status of src family members. T3M4 cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), and/or WPT-sTβRII (sTβRII) as indicated. Cell lysates were then analyzed with a phospho-kinase antibody array to assess the phosphorylation status of the indicated src family members. Results were quantified as described in Methods . Data are the means ± SEM of triplicate determinations from three independent experiments. *p

    Journal: PLoS ONE

    Article Title: Concomitant Targeting of EGF Receptor, TGF-beta and Src Points to a Novel Therapeutic Approach in Pancreatic Cancer

    doi: 10.1371/journal.pone.0039684

    Figure Lengend Snippet: Effects of targeting EGFR and TGF-β pathways on phosphorylation status of src family members. T3M4 cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), and/or WPT-sTβRII (sTβRII) as indicated. Cell lysates were then analyzed with a phospho-kinase antibody array to assess the phosphorylation status of the indicated src family members. Results were quantified as described in Methods . Data are the means ± SEM of triplicate determinations from three independent experiments. *p

    Article Snippet: Authenticity was confirmed by sequencing, and sTβRII expression was assessed by immunoblotting for HA (Cell Signaling, Danvers, MA).

    Techniques: Infection, Ab Array

    Effects of EGFR knockdown and sTβRII expression on receptor phosphorylation and downstream signaling. (A) Effects on receptor phosphorylation. ASPC-1 and T3M4 cells were infected as indicated with shLuc-LV (shLuc), shEGFR-LV (shEGFR), WPT-sTβRII (sTβRII), or both shEGFR and sTβRII. Cell lysates were subjected to immunoblotting with antibodies directed against the indicated receptors and phospho-receptors. (B) Cells were infected as indicated in A, and cell lysates were subjected to immunoblotting with antibodies directed against the indicated proteins and phospho-proteins. Each panel shows data from a representative of at least two independent experiments. In both panels A and B, immnoblotting with an anti-ERK antibody confirmed equivalent lane loading, but not all ERK blots are shown.

    Journal: PLoS ONE

    Article Title: Concomitant Targeting of EGF Receptor, TGF-beta and Src Points to a Novel Therapeutic Approach in Pancreatic Cancer

    doi: 10.1371/journal.pone.0039684

    Figure Lengend Snippet: Effects of EGFR knockdown and sTβRII expression on receptor phosphorylation and downstream signaling. (A) Effects on receptor phosphorylation. ASPC-1 and T3M4 cells were infected as indicated with shLuc-LV (shLuc), shEGFR-LV (shEGFR), WPT-sTβRII (sTβRII), or both shEGFR and sTβRII. Cell lysates were subjected to immunoblotting with antibodies directed against the indicated receptors and phospho-receptors. (B) Cells were infected as indicated in A, and cell lysates were subjected to immunoblotting with antibodies directed against the indicated proteins and phospho-proteins. Each panel shows data from a representative of at least two independent experiments. In both panels A and B, immnoblotting with an anti-ERK antibody confirmed equivalent lane loading, but not all ERK blots are shown.

    Article Snippet: Authenticity was confirmed by sequencing, and sTβRII expression was assessed by immunoblotting for HA (Cell Signaling, Danvers, MA).

    Techniques: Expressing, Infection

    Targeting EGFR and TGF-β pathways exerts different effects on the formation and growth of tumors formed by ASPC-1 and T3M4 cells. ASPC-1 (A) and T3M4 (B) cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), sTβRII, or both EGFR-LV and sTβRII, and injected subcutaneously (one injection per mouse) into the flank region of nude mice. Tumor volumes were monitored for the indicated number of days. Values are the means ± SEM of 8 mice per group, indicated in the denominator to the right of each curve. The number of tumors that formed in each group is indicated in the numerator. *p

    Journal: PLoS ONE

    Article Title: Concomitant Targeting of EGF Receptor, TGF-beta and Src Points to a Novel Therapeutic Approach in Pancreatic Cancer

    doi: 10.1371/journal.pone.0039684

    Figure Lengend Snippet: Targeting EGFR and TGF-β pathways exerts different effects on the formation and growth of tumors formed by ASPC-1 and T3M4 cells. ASPC-1 (A) and T3M4 (B) cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), sTβRII, or both EGFR-LV and sTβRII, and injected subcutaneously (one injection per mouse) into the flank region of nude mice. Tumor volumes were monitored for the indicated number of days. Values are the means ± SEM of 8 mice per group, indicated in the denominator to the right of each curve. The number of tumors that formed in each group is indicated in the numerator. *p

    Article Snippet: Authenticity was confirmed by sequencing, and sTβRII expression was assessed by immunoblotting for HA (Cell Signaling, Danvers, MA).

    Techniques: Infection, Injection, Mouse Assay

    EGFR knockdown and sTβRII expression modulate colony formation in pancreatic cancer cells. (A) ASPC-1 and T3M4 human pancreatic cancer cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), WPT-sTβRII (sTβRII), or both shEGFR and sTβRII. Cell lysates and conditioned media were then subjected to immunoblotting with anti-EGFR and anti-HA-tag antibodies, respectively, the latter serving to confirm sTβRII release by the cancer cells. An anti-ERK antibody served to assess lane loading. (B) The consequences of EGFR silencing with shEGFR and TGF-β sequestration with sTβRII were assessed by monitoring colony formation in 3-D culture (B). Data are the means ± SE of triplicate determinations from three independent experiments. *p

    Journal: PLoS ONE

    Article Title: Concomitant Targeting of EGF Receptor, TGF-beta and Src Points to a Novel Therapeutic Approach in Pancreatic Cancer

    doi: 10.1371/journal.pone.0039684

    Figure Lengend Snippet: EGFR knockdown and sTβRII expression modulate colony formation in pancreatic cancer cells. (A) ASPC-1 and T3M4 human pancreatic cancer cells were infected with shLuc-LV (shLuc), shEGFR-LV (shEGFR), WPT-sTβRII (sTβRII), or both shEGFR and sTβRII. Cell lysates and conditioned media were then subjected to immunoblotting with anti-EGFR and anti-HA-tag antibodies, respectively, the latter serving to confirm sTβRII release by the cancer cells. An anti-ERK antibody served to assess lane loading. (B) The consequences of EGFR silencing with shEGFR and TGF-β sequestration with sTβRII were assessed by monitoring colony formation in 3-D culture (B). Data are the means ± SE of triplicate determinations from three independent experiments. *p

    Article Snippet: Authenticity was confirmed by sequencing, and sTβRII expression was assessed by immunoblotting for HA (Cell Signaling, Danvers, MA).

    Techniques: Expressing, Infection