Journal: Biomolecules & Therapeutics

Article Title: Picropodophyllotoxin Inhibits Cell Growth and Induces Apoptosis in Gefitinib-Resistant Non-Small Lung Cancer Cells by Dual-Targeting EGFR and MET

doi: 10.4062/biomolther.2022.113

Figure Lengend Snippet: Effects of PPT on EGFR or MET mediated signaling pathways. Cells were treated with PPT at the indicated concentration for 48 h, then protein expression levels were determined. HCC827GR cell lysates were subjected to western blotting to detect of p-EGFR (Tyr1068), EGFR, p-MET (Tyr1234/1235), MET, p-AKT (Ser473), AKT, p-ERK (Thr202/Tyr204) and ERK antibodies.

Article Snippet: Antibodies against p-EGFR (Tyr1068), EGFR, p-MET (Tyr1234/1235), MET, p-AKT (Ser473), AKT, p-ERK (Thr202/Tyr204), and ERK were purchased from Cell Signaling Biotechnology (Beverly, MA, USA).

Techniques: Concentration Assay, Expressing, Western Blot

Journal: EMBO Molecular Medicine

Article Title: PM2 .5 promotes lung cancer progression through activation of the AhR‐TMPRSS2‐IL18 pathway

doi: 10.15252/emmm.202217014

Figure Lengend Snippet: A, B H1975 and A549 cells were treated with PM2.5 at 50 μg/ml for 90 days, and the proliferation of the treated cells was assessed by Trypan blue assays. C, D H1975 and A549 cells were exposed to PM2.5 at 50 μg/ml for different lengths of time, and the cell lysates of treated cells were assessed for phosphorylated EGFR (pEGFR) and EGFR by Western blotting. β‐actin served as the loading control. E, F H1975 and A549 cells were treated with PM2.5 at 50 μg/ml for 90 days. The anchorage‐independent growth was assessed by a soft agar colony formation assay. The number of colonies was scored, and the data are presented as the relative colony formation ability. Data information: The data shown are the means ± SDs from three independent experiments. * P < 0.05 and ** P < 0.01, compared with untreated control cells. The results shown in (C and D) are from one of three similar experiments. (A and B) P ‐values were determined by two‐sample t ‐test. (E and F) P ‐values were determined by one‐sample t ‐test. Source data are available online for this figure.

Article Snippet: Antibodies against phosphor (P)‐EGFR (1:1,000), EGFR (1:1,000), pSTAT3 (1:1,000), STAT3 (1:1,000), pAKT (1:1,000), pERK (1:1,000), IL‐18 (1:200), vimentin (1:1,000), and Ki‐67 (1:1,000) were purchased from Cell Signaling (Temecula, CA, USA).

Techniques: Western Blot, Soft Agar Assay