antibody against bk ca β1 apc 036  (Alomone Labs)


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    Alomone Labs antibody against bk ca β1 apc 036
    <t>BK</t> <t>Ca</t> <t>-β1</t> expression is reduced in the carotid arteries of rat balloon-injured model. Representative images of H&E (A) and immunohistochemical staining of BK Ca −α and BK ca −β1 (B) in injured and sham-operated carotid arteries of rat balloon-injured model at 14 days post injury. Scale bar = 100 μm. (C) Quantified mRNA levels of BK ca −α and BK ca −β1 analyzed by qRT-PCR. Western blot images (D) and quantification (E) for indicated proteins. Eukaryotic initiation factor 5 (eIF5) was used as an internal control. Data are presented as means ± SEM ( n = 4∼6 for each group, ns, not significant, * P < 0.05, ** P < 0.01 vs. sham).
    Antibody Against Bk Ca β1 Apc 036, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against bk ca β1 apc 036/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against bk ca β1 apc 036 - by Bioz Stars, 2023-03
    93/100 stars

    Images

    1) Product Images from "Large-conductance Ca 2 + -activated K + channel β1-subunit maintains the contractile phenotype of vascular smooth muscle cells"

    Article Title: Large-conductance Ca 2 + -activated K + channel β1-subunit maintains the contractile phenotype of vascular smooth muscle cells

    Journal: Frontiers in Cardiovascular Medicine

    doi: 10.3389/fcvm.2022.1062695

    BK Ca -β1 expression is reduced in the carotid arteries of rat balloon-injured model. Representative images of H&E (A) and immunohistochemical staining of BK Ca −α and BK ca −β1 (B) in injured and sham-operated carotid arteries of rat balloon-injured model at 14 days post injury. Scale bar = 100 μm. (C) Quantified mRNA levels of BK ca −α and BK ca −β1 analyzed by qRT-PCR. Western blot images (D) and quantification (E) for indicated proteins. Eukaryotic initiation factor 5 (eIF5) was used as an internal control. Data are presented as means ± SEM ( n = 4∼6 for each group, ns, not significant, * P < 0.05, ** P < 0.01 vs. sham).
    Figure Legend Snippet: BK Ca -β1 expression is reduced in the carotid arteries of rat balloon-injured model. Representative images of H&E (A) and immunohistochemical staining of BK Ca −α and BK ca −β1 (B) in injured and sham-operated carotid arteries of rat balloon-injured model at 14 days post injury. Scale bar = 100 μm. (C) Quantified mRNA levels of BK ca −α and BK ca −β1 analyzed by qRT-PCR. Western blot images (D) and quantification (E) for indicated proteins. Eukaryotic initiation factor 5 (eIF5) was used as an internal control. Data are presented as means ± SEM ( n = 4∼6 for each group, ns, not significant, * P < 0.05, ** P < 0.01 vs. sham).

    Techniques Used: Expressing, Immunohistochemical staining, Staining, Quantitative RT-PCR, Western Blot

    BK Ca -β1 expression is positively associated with VSMC differentiation markers in vitro . Western blot images (A) and quantification (B) of BK ca −α, BK ca −β1, and VSMC differentiation markers expression in VSMCs of passage 1 and passage 6. Western blot images (C) and quantification (D) of BK ca −α, BK ca −β1, and VSMC differentiation markers expression in VSMCs after PDGF-BB treatment (25 μg/L). Western blot images (E) and quantification (F) of BK ca −α, BK ca −β1, and VSMC differentiation markers expression in VSMCs treated with TGF-β (2.5 μg/L). VSMCs were subjected to serum-starvation for 24 h and then treated with the indicated stimulation for 48 h. Passages 3-5 of VSMCs were used in (C) through (F) . Eukaryotic initiation factor 5 (eIF5) was used as an internal control. Data are presented as means ± SEM ( n = 5 for each group, ns, not significant, * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. control).
    Figure Legend Snippet: BK Ca -β1 expression is positively associated with VSMC differentiation markers in vitro . Western blot images (A) and quantification (B) of BK ca −α, BK ca −β1, and VSMC differentiation markers expression in VSMCs of passage 1 and passage 6. Western blot images (C) and quantification (D) of BK ca −α, BK ca −β1, and VSMC differentiation markers expression in VSMCs after PDGF-BB treatment (25 μg/L). Western blot images (E) and quantification (F) of BK ca −α, BK ca −β1, and VSMC differentiation markers expression in VSMCs treated with TGF-β (2.5 μg/L). VSMCs were subjected to serum-starvation for 24 h and then treated with the indicated stimulation for 48 h. Passages 3-5 of VSMCs were used in (C) through (F) . Eukaryotic initiation factor 5 (eIF5) was used as an internal control. Data are presented as means ± SEM ( n = 5 for each group, ns, not significant, * P < 0.05, ** P < 0.01, and *** P < 0.001 vs. control).

    Techniques Used: Expressing, In Vitro, Western Blot

    Decreased BK ca −β1 expression is mediated by elevated PDGF-BB in vivo . (A) Quantified mRNA levels of PDGF-B and TGF-β analyzed by qRT-PCR in injured and sham-operated carotid arteries. Western blot images (B) and quantification (C) of BK ca −α and BK ca −β1 in the carotid arteries of mice injected with saline or PDGF-BB via tail vein. Data are presented as means ± SEM ( n = 6 for each group, ns, not significant, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. control).
    Figure Legend Snippet: Decreased BK ca −β1 expression is mediated by elevated PDGF-BB in vivo . (A) Quantified mRNA levels of PDGF-B and TGF-β analyzed by qRT-PCR in injured and sham-operated carotid arteries. Western blot images (B) and quantification (C) of BK ca −α and BK ca −β1 in the carotid arteries of mice injected with saline or PDGF-BB via tail vein. Data are presented as means ± SEM ( n = 6 for each group, ns, not significant, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. control).

    Techniques Used: Expressing, In Vivo, Quantitative RT-PCR, Western Blot, Injection

    BK Ca -β1 is essential for VSMCs to maintain a differentiated phenotype. (A) Representative micrographs of VSMCs treated with BK ca −β1 siRNA or scramble siRNA transfection. (B) Representative immunofluorescent images of F-actin of VSMCs stained with phalloidin (red). Nuclei were stained with DAPI (blue). Scale bar = 25 μm. (C) The percentage of spindle-like or polygonal-like cells in each group. Western blot images (D) and quantification (E) of BK ca −β1, α-SMA, and SM22α. Representative images (F) and quantification (G) of the areas of collagen gel in each group. The size of the collagen gel in each well was calculated compared to the total area of the well. Data are presented as means ± SEM ( n = 5∼6 for each group, *** P < 0.001 vs. scramble).
    Figure Legend Snippet: BK Ca -β1 is essential for VSMCs to maintain a differentiated phenotype. (A) Representative micrographs of VSMCs treated with BK ca −β1 siRNA or scramble siRNA transfection. (B) Representative immunofluorescent images of F-actin of VSMCs stained with phalloidin (red). Nuclei were stained with DAPI (blue). Scale bar = 25 μm. (C) The percentage of spindle-like or polygonal-like cells in each group. Western blot images (D) and quantification (E) of BK ca −β1, α-SMA, and SM22α. Representative images (F) and quantification (G) of the areas of collagen gel in each group. The size of the collagen gel in each well was calculated compared to the total area of the well. Data are presented as means ± SEM ( n = 5∼6 for each group, *** P < 0.001 vs. scramble).

    Techniques Used: Transfection, Staining, Western Blot

    BK Ca -β1 knockdown induces proliferative, migratory, and synthetic phenotype of VSMCs. (A) Proliferation curves of VSMCs transfected with BK ca −β1 or scrambled siRNA. Representative images (B) and quantification (C) of migration assay. (D) Quantified mRNA levels of inflammatory factors analyzed by qRT-PCR. Representative images (E) and quantification (F) of activities of MMP-9 and MMP-2 tested using gelatin zymography. Data are presented as means ± SEM ( n = 5∼6 for each group, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. scramble).
    Figure Legend Snippet: BK Ca -β1 knockdown induces proliferative, migratory, and synthetic phenotype of VSMCs. (A) Proliferation curves of VSMCs transfected with BK ca −β1 or scrambled siRNA. Representative images (B) and quantification (C) of migration assay. (D) Quantified mRNA levels of inflammatory factors analyzed by qRT-PCR. Representative images (E) and quantification (F) of activities of MMP-9 and MMP-2 tested using gelatin zymography. Data are presented as means ± SEM ( n = 5∼6 for each group, * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001 vs. scramble).

    Techniques Used: Transfection, Migration, Quantitative RT-PCR, Zymography

    Decreased BK ca −β1 expression is correlated with VSMC dedifferentiation and atherosclerosis in human. Western blot images (A) and quantification (B) of BK ca −α and BK ca −β1 expression in human primary aortic VSMCs after PDGF-BB treatment (25 μg/L). Western blot images (C) and quantification (D) of BK ca −α and BK ca −β1 expression in human primary aortic VSMCs treated with TGF-β (2.5 μg/L). VSMCs were subjected to serum-starvation for 24 h and then treated with the indicated stimulation for 48 h. Passages 3–5 of VSMCs were used. (E) Representative western blot images of the protein levels of BK ca −α and BK ca −β1 in lysates of human carotid endarterectomy artery (CEA) and control internal mammary arteries (IMA). (F) Quantification was performed by calculating the ratio between the levels of BK ca −β1 and BK ca −α. Eukaryotic initiation factor 5 (eIF5) was used as an internal control. Data are presented as means ± SEM (n = 3∼5 for each group, ns, not significant, * P < 0.05, ** P < 0.01 vs. control).
    Figure Legend Snippet: Decreased BK ca −β1 expression is correlated with VSMC dedifferentiation and atherosclerosis in human. Western blot images (A) and quantification (B) of BK ca −α and BK ca −β1 expression in human primary aortic VSMCs after PDGF-BB treatment (25 μg/L). Western blot images (C) and quantification (D) of BK ca −α and BK ca −β1 expression in human primary aortic VSMCs treated with TGF-β (2.5 μg/L). VSMCs were subjected to serum-starvation for 24 h and then treated with the indicated stimulation for 48 h. Passages 3–5 of VSMCs were used. (E) Representative western blot images of the protein levels of BK ca −α and BK ca −β1 in lysates of human carotid endarterectomy artery (CEA) and control internal mammary arteries (IMA). (F) Quantification was performed by calculating the ratio between the levels of BK ca −β1 and BK ca −α. Eukaryotic initiation factor 5 (eIF5) was used as an internal control. Data are presented as means ± SEM (n = 3∼5 for each group, ns, not significant, * P < 0.05, ** P < 0.01 vs. control).

    Techniques Used: Expressing, Western Blot

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