antibodies against vegf  (Thermo Fisher)


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    Name:
    VEGF Polyclonal Antibody
    Description:
    VEGF Polyclonal Antibody for Western Blot
    Catalog Number:
    opa110110
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher antibodies against vegf
    Heterogeneous <t>VEGF,</t> <t>VEGFR-1</t> and VEGFR-2 expression in colorectal cancer (CRC) endothelial cells (ECs): VEGF expression in ECs was heterogeneous with an labelling index (LI) range from 10.9 to 90% ( A , D ); Anti-VEGFR-1 immunostaining shows rare positivity with an LI range from nearly 0 ( B ) to 20% ( E ) in endothelial cells. VEGFR-2 shows a much wider immunostaining, with an LI range from 10 ( C ) to 72% ( F ). (Magnification: 400×). The arrows are pointed at vessels.
    VEGF Polyclonal Antibody for Western Blot
    https://www.bioz.com/result/antibodies against vegf/product/Thermo Fisher
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against vegf - by Bioz Stars, 2020-09
    96/100 stars

    Images

    1) Product Images from "The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer"

    Article Title: The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19113536

    Heterogeneous VEGF, VEGFR-1 and VEGFR-2 expression in colorectal cancer (CRC) endothelial cells (ECs): VEGF expression in ECs was heterogeneous with an labelling index (LI) range from 10.9 to 90% ( A , D ); Anti-VEGFR-1 immunostaining shows rare positivity with an LI range from nearly 0 ( B ) to 20% ( E ) in endothelial cells. VEGFR-2 shows a much wider immunostaining, with an LI range from 10 ( C ) to 72% ( F ). (Magnification: 400×). The arrows are pointed at vessels.
    Figure Legend Snippet: Heterogeneous VEGF, VEGFR-1 and VEGFR-2 expression in colorectal cancer (CRC) endothelial cells (ECs): VEGF expression in ECs was heterogeneous with an labelling index (LI) range from 10.9 to 90% ( A , D ); Anti-VEGFR-1 immunostaining shows rare positivity with an LI range from nearly 0 ( B ) to 20% ( E ) in endothelial cells. VEGFR-2 shows a much wider immunostaining, with an LI range from 10 ( C ) to 72% ( F ). (Magnification: 400×). The arrows are pointed at vessels.

    Techniques Used: Expressing, Immunostaining

    Quantitative image analysis on Tissue MicroArray (TMA): ( A ) After digitization of the TMA slides with a NanoZoomer Digital Slide Scanner (Hamamatsu, Hamamatsu City, Japan), blood vessels, highlighted by anti-CD34 immunostaining, were manually marked on the digital vascular endothelial growth factor receptor (VEGFR) slides by a pathologist using the NDP.view software. The tissue core diameter is 600 µm. ( B ) The delineated regions were imported into the Visiopharm software to quantify the VEGF, VEGFR-1 or VEGFR-2 expression levels using the labelling index (LI), i.e., the percentage of the immunoreactive area within the vessel area.
    Figure Legend Snippet: Quantitative image analysis on Tissue MicroArray (TMA): ( A ) After digitization of the TMA slides with a NanoZoomer Digital Slide Scanner (Hamamatsu, Hamamatsu City, Japan), blood vessels, highlighted by anti-CD34 immunostaining, were manually marked on the digital vascular endothelial growth factor receptor (VEGFR) slides by a pathologist using the NDP.view software. The tissue core diameter is 600 µm. ( B ) The delineated regions were imported into the Visiopharm software to quantify the VEGF, VEGFR-1 or VEGFR-2 expression levels using the labelling index (LI), i.e., the percentage of the immunoreactive area within the vessel area.

    Techniques Used: Microarray, Immunostaining, Software, Expressing

    2) Product Images from "Effective Wound Healing Enabled by Discrete Alternative Electric Fields from Wearable Nanogenerators"

    Article Title: Effective Wound Healing Enabled by Discrete Alternative Electric Fields from Wearable Nanogenerators

    Journal: ACS Nano

    doi: 10.1021/acsnano.8b07038

    Influence of electric field on cells. (a) Schematic image of cells cultured in 96-well plate with stimulation from NG and cell viability of different columns in a 96-well plate ( n = 8). (b) Schematic image of cells cultured in a dish with Au electrodes connected and disconnected to NG generated pulse voltage. Middle inset is the simulated electric filed distribution in the culture dish when the electrodes are connected to a NG with ±2 V out voltage. (c) Cultured cell morphology at different time points without (control) and with (experimental) electrical stimulation. Obvious proliferation and differentiation of cells at later time points were observed. (d) Western blot analysis and comparison of TGF-β, EGF, and VEGF growth factors ( n = 3) with and without NG stimulation. (e) ROS results of blank control (BC), AC (alternating electric field generated by function generator), and NG ( n = 5, p
    Figure Legend Snippet: Influence of electric field on cells. (a) Schematic image of cells cultured in 96-well plate with stimulation from NG and cell viability of different columns in a 96-well plate ( n = 8). (b) Schematic image of cells cultured in a dish with Au electrodes connected and disconnected to NG generated pulse voltage. Middle inset is the simulated electric filed distribution in the culture dish when the electrodes are connected to a NG with ±2 V out voltage. (c) Cultured cell morphology at different time points without (control) and with (experimental) electrical stimulation. Obvious proliferation and differentiation of cells at later time points were observed. (d) Western blot analysis and comparison of TGF-β, EGF, and VEGF growth factors ( n = 3) with and without NG stimulation. (e) ROS results of blank control (BC), AC (alternating electric field generated by function generator), and NG ( n = 5, p

    Techniques Used: Cell Culture, Generated, Western Blot

    3) Product Images from "Everolimus as an mTOR Inhibitor Suppresses Endometriotic Implants: an Experimental Rat Study"

    Article Title: Everolimus as an mTOR Inhibitor Suppresses Endometriotic Implants: an Experimental Rat Study

    Journal: Geburtshilfe und Frauenheilkunde

    doi: 10.1055/s-0042-115566

    VEGF, CD117, and Bax staining scores of rats administered normal saline, anastrazole, and everolimus. Data were expressed as mean ± SEM. a, b p
    Figure Legend Snippet: VEGF, CD117, and Bax staining scores of rats administered normal saline, anastrazole, and everolimus. Data were expressed as mean ± SEM. a, b p

    Techniques Used: Staining

    Representative immunohistochemical staining (VEGF, CD117, Bax) images of endometriotic lesions in rats administered normal saline, anastrozole, and everolimus. Scale bars, 200 px in all images (1280 px in width).
    Figure Legend Snippet: Representative immunohistochemical staining (VEGF, CD117, Bax) images of endometriotic lesions in rats administered normal saline, anastrozole, and everolimus. Scale bars, 200 px in all images (1280 px in width).

    Techniques Used: Immunohistochemistry, Staining

    4) Product Images from "Effective Wound Healing Enabled by Discrete Alternative Electric Fields from Wearable Nanogenerators"

    Article Title: Effective Wound Healing Enabled by Discrete Alternative Electric Fields from Wearable Nanogenerators

    Journal: ACS Nano

    doi: 10.1021/acsnano.8b07038

    Influence of electric field on cells. (a) Schematic image of cells cultured in 96-well plate with stimulation from NG and cell viability of different columns in a 96-well plate ( n = 8). (b) Schematic image of cells cultured in a dish with Au electrodes connected and disconnected to NG generated pulse voltage. Middle inset is the simulated electric filed distribution in the culture dish when the electrodes are connected to a NG with ±2 V out voltage. (c) Cultured cell morphology at different time points without (control) and with (experimental) electrical stimulation. Obvious proliferation and differentiation of cells at later time points were observed. (d) Western blot analysis and comparison of TGF-β, EGF, and VEGF growth factors ( n = 3) with and without NG stimulation. (e) ROS results of blank control (BC), AC (alternating electric field generated by function generator), and NG ( n = 5, p
    Figure Legend Snippet: Influence of electric field on cells. (a) Schematic image of cells cultured in 96-well plate with stimulation from NG and cell viability of different columns in a 96-well plate ( n = 8). (b) Schematic image of cells cultured in a dish with Au electrodes connected and disconnected to NG generated pulse voltage. Middle inset is the simulated electric filed distribution in the culture dish when the electrodes are connected to a NG with ±2 V out voltage. (c) Cultured cell morphology at different time points without (control) and with (experimental) electrical stimulation. Obvious proliferation and differentiation of cells at later time points were observed. (d) Western blot analysis and comparison of TGF-β, EGF, and VEGF growth factors ( n = 3) with and without NG stimulation. (e) ROS results of blank control (BC), AC (alternating electric field generated by function generator), and NG ( n = 5, p

    Techniques Used: Cell Culture, Generated, Western Blot

    5) Product Images from "BLCA1 expression is associated with angiogenesis of bladder cancer and is correlated with common pro-angiogenic factors"

    Article Title: BLCA1 expression is associated with angiogenesis of bladder cancer and is correlated with common pro-angiogenic factors

    Journal: International Journal of Clinical and Experimental Medicine

    doi:

    Western blotting showingthe (A) specific band of BLCA1 in a dose-dependent manner; Immunopositive staining of (B) IL1α, (C) BLCA1, (D) VEGF, (E) IL8, (F) BLCA4, (G) MMP9, and (H) TNFα in bladder cancer.
    Figure Legend Snippet: Western blotting showingthe (A) specific band of BLCA1 in a dose-dependent manner; Immunopositive staining of (B) IL1α, (C) BLCA1, (D) VEGF, (E) IL8, (F) BLCA4, (G) MMP9, and (H) TNFα in bladder cancer.

    Techniques Used: Western Blot, Staining

    6) Product Images from "The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer"

    Article Title: The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms19113536

    Heterogeneous VEGF, VEGFR-1 and VEGFR-2 expression in colorectal cancer (CRC) endothelial cells (ECs): VEGF expression in ECs was heterogeneous with an labelling index (LI) range from 10.9 to 90% ( A , D ); Anti-VEGFR-1 immunostaining shows rare positivity with an LI range from nearly 0 ( B ) to 20% ( E ) in endothelial cells. VEGFR-2 shows a much wider immunostaining, with an LI range from 10 ( C ) to 72% ( F ). (Magnification: 400×). The arrows are pointed at vessels.
    Figure Legend Snippet: Heterogeneous VEGF, VEGFR-1 and VEGFR-2 expression in colorectal cancer (CRC) endothelial cells (ECs): VEGF expression in ECs was heterogeneous with an labelling index (LI) range from 10.9 to 90% ( A , D ); Anti-VEGFR-1 immunostaining shows rare positivity with an LI range from nearly 0 ( B ) to 20% ( E ) in endothelial cells. VEGFR-2 shows a much wider immunostaining, with an LI range from 10 ( C ) to 72% ( F ). (Magnification: 400×). The arrows are pointed at vessels.

    Techniques Used: Expressing, Immunostaining

    Quantitative image analysis on Tissue MicroArray (TMA): ( A ) After digitization of the TMA slides with a NanoZoomer Digital Slide Scanner (Hamamatsu, Hamamatsu City, Japan), blood vessels, highlighted by anti-CD34 immunostaining, were manually marked on the digital vascular endothelial growth factor receptor (VEGFR) slides by a pathologist using the NDP.view software. The tissue core diameter is 600 µm. ( B ) The delineated regions were imported into the Visiopharm software to quantify the VEGF, VEGFR-1 or VEGFR-2 expression levels using the labelling index (LI), i.e., the percentage of the immunoreactive area within the vessel area.
    Figure Legend Snippet: Quantitative image analysis on Tissue MicroArray (TMA): ( A ) After digitization of the TMA slides with a NanoZoomer Digital Slide Scanner (Hamamatsu, Hamamatsu City, Japan), blood vessels, highlighted by anti-CD34 immunostaining, were manually marked on the digital vascular endothelial growth factor receptor (VEGFR) slides by a pathologist using the NDP.view software. The tissue core diameter is 600 µm. ( B ) The delineated regions were imported into the Visiopharm software to quantify the VEGF, VEGFR-1 or VEGFR-2 expression levels using the labelling index (LI), i.e., the percentage of the immunoreactive area within the vessel area.

    Techniques Used: Microarray, Immunostaining, Software, Expressing

    Related Articles

    Immunohistochemistry:

    Article Title: The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer
    Article Snippet: .. Immunohistochemistry Standard immunohistochemistry (IHC) was applied as previously described [ , ] to serial 5-μm-thick sections to display VEGF, VEGFR-1 and VEGFR-2 expression using antibodies against VEGF (Thermo Scientific, Merelbeke, Belgium, polyclonal, dilution:1:50), VEGFR-1 (Abcam, Cambridge, UK, clone Y103, dilution 1:50) and VEGFR-2 (Cell Signaling, Danvers, MA, USA, clone 55B11, dilution 1:100). .. An antibody against CD34 (Abcam, Cambridge, UK, clone QBEND-10, dilution 1:200) was used to identify blood vessels.

    Article Title: The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer
    Article Snippet: .. Standard immunohistochemistry (IHC) was applied as previously described [ , ] to serial 5-μm-thick sections to display VEGF, VEGFR-1 and VEGFR-2 expression using antibodies against VEGF (Thermo Scientific, Merelbeke, Belgium, polyclonal, dilution:1:50), VEGFR-1 (Abcam, Cambridge, UK, clone Y103, dilution 1:50) and VEGFR-2 (Cell Signaling, Danvers, MA, USA, clone 55B11, dilution 1:100). .. An antibody against CD34 (Abcam, Cambridge, UK, clone QBEND-10, dilution 1:200) was used to identify blood vessels.

    Mouse Assay:

    Article Title: HIF-1α siRNA reduces retinal neovascularization in a mouse model of retinopathy of prematurity
    Article Snippet: .. The following materials were used in this study: C57BL/6J mice provided by Shanghai Experimental Animal Center of Chinese Academy of Sciences; cationic liposomes (LipofectamineTM 2000, LF2000) purchased from Invitrogen; goat anti-VEGF polyclonal antibodies purchased from R & D; goat anti-HIF-1α polyclonal antibody purchased Santa Cruz; and mouse anti-β-actin monoclonal antibody and FITC-Dextran (MW 2×106 ) purchased from Sigma. .. The small interfering RNA target was designed according to the sequence of HIF-1α gene ( ) in the human gene pool, with the target sequence of AAGAGGTGGATATGTCTGG (1214-1232).

    Concentration Assay:

    Article Title: Proteasome Assay in Cell Lysates
    Article Snippet: .. Cells HEPES (Sigma-Aldrich, catalog number: H3375) MgCl2 (Sigma-Aldrich, catalog number: M2670) EDTA (Sigma-Aldrich, catalog number: E5134) EGTA (Sigma-Aldrich, catalog number: E4378) Sucrose (MP Biomedicals, catalog number: 821713) DTT (Life Technologies, catalog number: 15508013) Proteasome substrates [dissolved in DMSO (Sigma-Aldrich, catalog number: D8418) to a final concentration of 10 mM and stored frozen at −20 °C] Suc-LLVY-AMC (chymotrypsin-like activity substrate) (Enzo Life Sciences, catalog number: P802) Z-ARR-AMC (trypsin-like activity substrate) (EMD Millipore, catalog number: 539149) Z-LLE-AMC (caspase-like activity substrate) (EMD Millipore, catalog number: 539141) PBS without Ca2+ and Mg2+ (Sigma-Aldrich, catalog number: P802) Coomassie Protein Assay Reagent (Thermo Fisher Scientific, catalog number: 1856209) BSA protein standard (Thermo Scientific, catalog number: 23209) ATP (Sigma-Aldrich, catalog number: A3377) Proteasome Lysis/Assay Buffer (see Recipes) .. 60 mm tissue culture dishes Rubber policeman or other type of cell scraper 1.7 ml microcentrifuge tube Black walled 96 well plates (Corning, Costar® , catalog number: 3603) Clear 96 well plates (Greiner Bio-One GmbH, catalog number: 655101) Sonicator (GlobalSpec, model: W-380) Microcentrifuge Fluorescent plate reader Visible plate reader

    Activity Assay:

    Article Title: Proteasome Assay in Cell Lysates
    Article Snippet: .. Cells HEPES (Sigma-Aldrich, catalog number: H3375) MgCl2 (Sigma-Aldrich, catalog number: M2670) EDTA (Sigma-Aldrich, catalog number: E5134) EGTA (Sigma-Aldrich, catalog number: E4378) Sucrose (MP Biomedicals, catalog number: 821713) DTT (Life Technologies, catalog number: 15508013) Proteasome substrates [dissolved in DMSO (Sigma-Aldrich, catalog number: D8418) to a final concentration of 10 mM and stored frozen at −20 °C] Suc-LLVY-AMC (chymotrypsin-like activity substrate) (Enzo Life Sciences, catalog number: P802) Z-ARR-AMC (trypsin-like activity substrate) (EMD Millipore, catalog number: 539149) Z-LLE-AMC (caspase-like activity substrate) (EMD Millipore, catalog number: 539141) PBS without Ca2+ and Mg2+ (Sigma-Aldrich, catalog number: P802) Coomassie Protein Assay Reagent (Thermo Fisher Scientific, catalog number: 1856209) BSA protein standard (Thermo Scientific, catalog number: 23209) ATP (Sigma-Aldrich, catalog number: A3377) Proteasome Lysis/Assay Buffer (see Recipes) .. 60 mm tissue culture dishes Rubber policeman or other type of cell scraper 1.7 ml microcentrifuge tube Black walled 96 well plates (Corning, Costar® , catalog number: 3603) Clear 96 well plates (Greiner Bio-One GmbH, catalog number: 655101) Sonicator (GlobalSpec, model: W-380) Microcentrifuge Fluorescent plate reader Visible plate reader

    Expressing:

    Article Title: The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer
    Article Snippet: .. Immunohistochemistry Standard immunohistochemistry (IHC) was applied as previously described [ , ] to serial 5-μm-thick sections to display VEGF, VEGFR-1 and VEGFR-2 expression using antibodies against VEGF (Thermo Scientific, Merelbeke, Belgium, polyclonal, dilution:1:50), VEGFR-1 (Abcam, Cambridge, UK, clone Y103, dilution 1:50) and VEGFR-2 (Cell Signaling, Danvers, MA, USA, clone 55B11, dilution 1:100). .. An antibody against CD34 (Abcam, Cambridge, UK, clone QBEND-10, dilution 1:200) was used to identify blood vessels.

    Article Title: The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer
    Article Snippet: .. Standard immunohistochemistry (IHC) was applied as previously described [ , ] to serial 5-μm-thick sections to display VEGF, VEGFR-1 and VEGFR-2 expression using antibodies against VEGF (Thermo Scientific, Merelbeke, Belgium, polyclonal, dilution:1:50), VEGFR-1 (Abcam, Cambridge, UK, clone Y103, dilution 1:50) and VEGFR-2 (Cell Signaling, Danvers, MA, USA, clone 55B11, dilution 1:100). .. An antibody against CD34 (Abcam, Cambridge, UK, clone QBEND-10, dilution 1:200) was used to identify blood vessels.

    Staining:

    Article Title: Vascular Endothelial Growth Factor Enhances Glomerular Capillary Repair and Accelerates Resolution of Experimentally Induced Glomerulonephritis
    Article Snippet: .. Four-μm frozen sections were stained with polyclonal rabbit anti-VEGF antibody and followed by fluorescein isothiocyanate-labeled goat anti-rabbit IgG antibody (Zymed, San Francisco, CA). .. Sections were then incubated with anti-Thy-1 antibody (OX7, Cedarlane Laboratories) and followed by Texas Red-conjugated goat anti-mouse IgG antibody (Leinco Technologies, St. Louis, MO), and the nuclei were counterstained with 4,6-diamidino-2-phenylindole (DAPI; Vector Laboratories).

    Lysis:

    Article Title: Proteasome Assay in Cell Lysates
    Article Snippet: .. Cells HEPES (Sigma-Aldrich, catalog number: H3375) MgCl2 (Sigma-Aldrich, catalog number: M2670) EDTA (Sigma-Aldrich, catalog number: E5134) EGTA (Sigma-Aldrich, catalog number: E4378) Sucrose (MP Biomedicals, catalog number: 821713) DTT (Life Technologies, catalog number: 15508013) Proteasome substrates [dissolved in DMSO (Sigma-Aldrich, catalog number: D8418) to a final concentration of 10 mM and stored frozen at −20 °C] Suc-LLVY-AMC (chymotrypsin-like activity substrate) (Enzo Life Sciences, catalog number: P802) Z-ARR-AMC (trypsin-like activity substrate) (EMD Millipore, catalog number: 539149) Z-LLE-AMC (caspase-like activity substrate) (EMD Millipore, catalog number: 539141) PBS without Ca2+ and Mg2+ (Sigma-Aldrich, catalog number: P802) Coomassie Protein Assay Reagent (Thermo Fisher Scientific, catalog number: 1856209) BSA protein standard (Thermo Scientific, catalog number: 23209) ATP (Sigma-Aldrich, catalog number: A3377) Proteasome Lysis/Assay Buffer (see Recipes) .. 60 mm tissue culture dishes Rubber policeman or other type of cell scraper 1.7 ml microcentrifuge tube Black walled 96 well plates (Corning, Costar® , catalog number: 3603) Clear 96 well plates (Greiner Bio-One GmbH, catalog number: 655101) Sonicator (GlobalSpec, model: W-380) Microcentrifuge Fluorescent plate reader Visible plate reader

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  • 99
    Thermo Fisher antibodies against vegf
    Heterogeneous <t>VEGF,</t> <t>VEGFR-1</t> and VEGFR-2 expression in colorectal cancer (CRC) endothelial cells (ECs): VEGF expression in ECs was heterogeneous with an labelling index (LI) range from 10.9 to 90% ( A , D ); Anti-VEGFR-1 immunostaining shows rare positivity with an LI range from nearly 0 ( B ) to 20% ( E ) in endothelial cells. VEGFR-2 shows a much wider immunostaining, with an LI range from 10 ( C ) to 72% ( F ). (Magnification: 400×). The arrows are pointed at vessels.
    Antibodies Against Vegf, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against vegf/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against vegf - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Heterogeneous VEGF, VEGFR-1 and VEGFR-2 expression in colorectal cancer (CRC) endothelial cells (ECs): VEGF expression in ECs was heterogeneous with an labelling index (LI) range from 10.9 to 90% ( A , D ); Anti-VEGFR-1 immunostaining shows rare positivity with an LI range from nearly 0 ( B ) to 20% ( E ) in endothelial cells. VEGFR-2 shows a much wider immunostaining, with an LI range from 10 ( C ) to 72% ( F ). (Magnification: 400×). The arrows are pointed at vessels.

    Journal: International Journal of Molecular Sciences

    Article Title: The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer

    doi: 10.3390/ijms19113536

    Figure Lengend Snippet: Heterogeneous VEGF, VEGFR-1 and VEGFR-2 expression in colorectal cancer (CRC) endothelial cells (ECs): VEGF expression in ECs was heterogeneous with an labelling index (LI) range from 10.9 to 90% ( A , D ); Anti-VEGFR-1 immunostaining shows rare positivity with an LI range from nearly 0 ( B ) to 20% ( E ) in endothelial cells. VEGFR-2 shows a much wider immunostaining, with an LI range from 10 ( C ) to 72% ( F ). (Magnification: 400×). The arrows are pointed at vessels.

    Article Snippet: Immunohistochemistry Standard immunohistochemistry (IHC) was applied as previously described [ , ] to serial 5-μm-thick sections to display VEGF, VEGFR-1 and VEGFR-2 expression using antibodies against VEGF (Thermo Scientific, Merelbeke, Belgium, polyclonal, dilution:1:50), VEGFR-1 (Abcam, Cambridge, UK, clone Y103, dilution 1:50) and VEGFR-2 (Cell Signaling, Danvers, MA, USA, clone 55B11, dilution 1:100).

    Techniques: Expressing, Immunostaining

    Quantitative image analysis on Tissue MicroArray (TMA): ( A ) After digitization of the TMA slides with a NanoZoomer Digital Slide Scanner (Hamamatsu, Hamamatsu City, Japan), blood vessels, highlighted by anti-CD34 immunostaining, were manually marked on the digital vascular endothelial growth factor receptor (VEGFR) slides by a pathologist using the NDP.view software. The tissue core diameter is 600 µm. ( B ) The delineated regions were imported into the Visiopharm software to quantify the VEGF, VEGFR-1 or VEGFR-2 expression levels using the labelling index (LI), i.e., the percentage of the immunoreactive area within the vessel area.

    Journal: International Journal of Molecular Sciences

    Article Title: The Prognostic Value of the Combination of Low VEGFR-1 and High VEGFR-2 Expression in Endothelial Cells of Colorectal Cancer

    doi: 10.3390/ijms19113536

    Figure Lengend Snippet: Quantitative image analysis on Tissue MicroArray (TMA): ( A ) After digitization of the TMA slides with a NanoZoomer Digital Slide Scanner (Hamamatsu, Hamamatsu City, Japan), blood vessels, highlighted by anti-CD34 immunostaining, were manually marked on the digital vascular endothelial growth factor receptor (VEGFR) slides by a pathologist using the NDP.view software. The tissue core diameter is 600 µm. ( B ) The delineated regions were imported into the Visiopharm software to quantify the VEGF, VEGFR-1 or VEGFR-2 expression levels using the labelling index (LI), i.e., the percentage of the immunoreactive area within the vessel area.

    Article Snippet: Immunohistochemistry Standard immunohistochemistry (IHC) was applied as previously described [ , ] to serial 5-μm-thick sections to display VEGF, VEGFR-1 and VEGFR-2 expression using antibodies against VEGF (Thermo Scientific, Merelbeke, Belgium, polyclonal, dilution:1:50), VEGFR-1 (Abcam, Cambridge, UK, clone Y103, dilution 1:50) and VEGFR-2 (Cell Signaling, Danvers, MA, USA, clone 55B11, dilution 1:100).

    Techniques: Microarray, Immunostaining, Software, Expressing