Journal: Journal of Reproduction & Infertility

Article Title: The Association of Mitochondrial Translocator Protein and Voltage-Dependent Anion Channel-1 in Granulosa Cells with Estradiol Levels and Presence of Immature Follicles in Polycystic Ovary Syndrome

doi: 10.18502/jri.v23i3.10005

Figure Lengend Snippet: The relative gene expression and protein levels of mitochondrial membrane proteins, VDAC1 and TSPO, in controls and PCOS patients

Article Snippet: Next, 10% goat serum was added and the cells were incubated overnight with primary (Santa Cruz Biotechnology, USA, sc-390996) and secondary (Abcam, USA, ab6785) antibodies against VDAC1 and primary (Boster Bio, USA, m01153) and secondary (Abcam, USA, ab191866) antibodies against TSPO, and stored overnight at 4 °C in a humidity chamber.

Techniques: Expressing

Journal: Journal of Reproduction & Infertility

Article Title: The Association of Mitochondrial Translocator Protein and Voltage-Dependent Anion Channel-1 in Granulosa Cells with Estradiol Levels and Presence of Immature Follicles in Polycystic Ovary Syndrome

doi: 10.18502/jri.v23i3.10005

Figure Lengend Snippet: VDAC1 protein levels were obtained by immunocytochemistry where green fluorescence intensities indicated cytoplasmic VDAC1 and blue DAPI fluorescence showed nuclear counterstain. Green and blue intensities were merged to obtain intensity ratio of green/blue fluorescence corresponding to the relative cytoplasmic VDAC1 levels and quantified with ImageJ software

Article Snippet: Next, 10% goat serum was added and the cells were incubated overnight with primary (Santa Cruz Biotechnology, USA, sc-390996) and secondary (Abcam, USA, ab6785) antibodies against VDAC1 and primary (Boster Bio, USA, m01153) and secondary (Abcam, USA, ab191866) antibodies against TSPO, and stored overnight at 4 °C in a humidity chamber.

Techniques: Immunocytochemistry, Fluorescence, Software

Journal: Journal of Reproduction & Infertility

Article Title: The Association of Mitochondrial Translocator Protein and Voltage-Dependent Anion Channel-1 in Granulosa Cells with Estradiol Levels and Presence of Immature Follicles in Polycystic Ovary Syndrome

doi: 10.18502/jri.v23i3.10005

Figure Lengend Snippet: Correlation between concentration of estradiol (E2) in follicular fluid with the expression of (A) voltage-dependent anion channel 1 (VDAC1) and (B) translocator protein (TSPO) in GCs of 43 PCOS patients and 43 controls, as determined by Spearman’s correlation analysis

Article Snippet: Next, 10% goat serum was added and the cells were incubated overnight with primary (Santa Cruz Biotechnology, USA, sc-390996) and secondary (Abcam, USA, ab6785) antibodies against VDAC1 and primary (Boster Bio, USA, m01153) and secondary (Abcam, USA, ab191866) antibodies against TSPO, and stored overnight at 4 °C in a humidity chamber.

Techniques: Concentration Assay, Expressing

Journal: Journal of Reproduction & Infertility

Article Title: The Association of Mitochondrial Translocator Protein and Voltage-Dependent Anion Channel-1 in Granulosa Cells with Estradiol Levels and Presence of Immature Follicles in Polycystic Ovary Syndrome

doi: 10.18502/jri.v23i3.10005

Figure Lengend Snippet: Associations of oxidative stress markers of follicular fluids with TSPO and VDAC1 gene and protein levels in all subjects

Article Snippet: Next, 10% goat serum was added and the cells were incubated overnight with primary (Santa Cruz Biotechnology, USA, sc-390996) and secondary (Abcam, USA, ab6785) antibodies against VDAC1 and primary (Boster Bio, USA, m01153) and secondary (Abcam, USA, ab191866) antibodies against TSPO, and stored overnight at 4 °C in a humidity chamber.

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: Oxymatrine blocks the NLRP3 inflammasome pathway, partly downregulating the inflammatory responses of M1 macrophages differentiated from THP-1 monocytes

doi: 10.1016/j.bbrep.2023.101482

Figure Lengend Snippet: Morphological images of cells in different states and identification of M0 and M1 macrophages. (A) Morphology of THP-1 cells, M0 macrophages, and M1 macrophages. Scale bar: 200 μm. THP-1 monocytes were round, translucent, and suspended; M0 macrophages were clustered and adherent; M1 macrophages were adherent and can extend pseudopodias. (B) Immunofluorescence staining on M0 cells. Scale bar: 50 μm. Colocalization of CD68 (red) and DAPI (blue). (C) Immunofluorescence staining of M1 cells. Scale bar: 50 μm. Colocalization of CD86 (red) and DAPI (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: For immunofluorescence staining, the cells were fixed with 4% paraformaldehyde (PFA) (Biosharp, Anhui, China) and incubated with diluted rabbit primary antibody against CD68 (1:300) (Boster, Wuhan, China) overnight at 4 °C.

Techniques: Immunofluorescence, Staining

Journal: Biochemistry and Biophysics Reports

Article Title: Oxymatrine blocks the NLRP3 inflammasome pathway, partly downregulating the inflammatory responses of M1 macrophages differentiated from THP-1 monocytes ☆

doi: 10.1016/j.bbrep.2023.101482

Figure Lengend Snippet: Morphological images of cells in different states and identification of M0 and M1 macrophages. (A) Morphology of THP-1 cells, M0 macrophages, and M1 macrophages. Scale bar: 200 μm. THP-1 monocytes were round, translucent, and suspended; M0 macrophages were clustered and adherent; M1 macrophages were adherent and can extend pseudopodias. (B) Immunofluorescence staining on M0 cells. Scale bar: 50 μm. Colocalization of CD68 (red) and DAPI (blue). (C) Immunofluorescence staining of M1 cells. Scale bar: 50 μm. Colocalization of CD86 (red) and DAPI (blue). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: A rabbit primary antibody against CD86 (1:300) (Boster, Wuhan, China) was used to identify M1 cells using the protocol described in section .

Techniques: Immunofluorescence, Staining

Journal: BioMed Research International

Article Title: Quercetin Downregulates Cyclooxygenase-2 Expression and HIF-1 α /VEGF Signaling-Related Angiogenesis in a Mouse Model of Abdominal Aortic Aneurysm

doi: 10.1155/2020/9485398

Figure Lengend Snippet: The effect of quercetin and celecoxib on histology and CD31 + cells of CaCl 2 -induced AAAs. (a) Transverse sections of formalin-fixed aortic tissue were stained with Victoria blue (VB) (elastin in blue) and Masson's trichrome (MT) stain (collagen in blue). Sections were immunostained for CD31 to examine the microvessel density. Arrows indicate positive CD31 immunostaining. Scale bars are 50 μ m. Each section shown is a representative of five samples with similar results. (b) Percentage of area positive for elastic fibers. The area of elastic fibers in VB-stained sections was calculated by quantitative morphometric analysis with ImageJ. Results were expressed as a percentage of the area of the aortic media. (c) CD31 + microvessel contents in the mouse aortic wall ( n = 5 in each group). ∗ P < 0.05 vs. sham, # P < 0.05 vs. control.

Article Snippet: As described previously [ ], the slides were incubated with a rabbit antibody against CD31 (1 : 200, A01513-1, Boster, Wuhan, China) and subsequently horseradish peroxidase-conjugated goat antibodies against rabbit (sc-3837, Santa Cruz Biotechnology, Santa Cruz, CA, US).

Techniques: Staining, Immunostaining

Journal: PLoS ONE

Article Title: Transcriptome Sequencing Reveals Differences between Primary and Secondary Hair Follicle-derived Dermal Papilla Cells of the Cashmere Goat ( Capra hircus )

doi: 10.1371/journal.pone.0076282

Figure Lengend Snippet: Immunocytochemistry of DPCs using anti-α-SMA antibody (green) was performed (A&B) when cells started to exhibit an aggregative growth behavior, while the other two antibodies were performed on monolayer cultured DPCs. Both primary hair follicle-dermal papilla cells (PHF-DPCs) and secondary hair follicle-dermal papilla cells (SHF-DPCs) were positive for α-SMA (green, A & B), Laminin (red, C & D), and Collagen IV (red, E & F). Nuclei in A–F were marked by DAPI staining (blue). Scale bars = 100 µm.

Article Snippet: Excess BSA was removed and the coverslips were covered with a solution containing a mouse monoclonal antibody [1A4] against alpha smooth muscle Actin (α-SMA) antibody (FITC) (Abcam, Cambridge, MA, USA), a rabbit polyclonal antibody against laminin (Wuhan Boster Biotech, Wuhan, China) or a rabbit polyclonal antibody against COL4A1 (Wuhan Boster Biotech) at 1∶100 dilution in PBS and incubated overnight at 4°C.

Techniques: Immunocytochemistry, Cell Culture, Staining

Journal: PLoS ONE

Article Title: Transcriptome Sequencing Reveals Differences between Primary and Secondary Hair Follicle-derived Dermal Papilla Cells of the Cashmere Goat ( Capra hircus )

doi: 10.1371/journal.pone.0076282

Figure Lengend Snippet: Expression level of differentially expressed genes between PHF-DPCs and SHF-DPCs involved in ECM-receptor interaction pathway.

Article Snippet: Excess BSA was removed and the coverslips were covered with a solution containing a mouse monoclonal antibody [1A4] against alpha smooth muscle Actin (α-SMA) antibody (FITC) (Abcam, Cambridge, MA, USA), a rabbit polyclonal antibody against laminin (Wuhan Boster Biotech, Wuhan, China) or a rabbit polyclonal antibody against COL4A1 (Wuhan Boster Biotech) at 1∶100 dilution in PBS and incubated overnight at 4°C.

Techniques: Expressing