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antibodies against pik3c3 z r016  (Echelon Biosciences)


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    Echelon Biosciences antibodies against pik3c3 z r016
    Generation of a <t>Pik3c3</t> hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.
    Antibodies Against Pik3c3 Z R016, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against pik3c3 z r016/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against pik3c3 z r016 - by Bioz Stars, 2024-12
    92/100 stars

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    1) Product Images from "The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy"

    Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy

    Journal: American Journal of Physiology - Renal Physiology

    doi: 10.1152/ajprenal.00381.2019

    Generation of a Pik3c3 hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.
    Figure Legend Snippet: Generation of a Pik3c3 hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.

    Techniques Used: Plasmid Preparation, Western Blot, Immunohistochemistry, Staining

    The reduction of Pik3c3 expression also occurs in other organs of the Pik3c3 hypomorphic mouse model. Immunoblot analysis indicated that homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice (Homo) express a markedly lower level of Pik3c3 than heterozygous Pik3c3 hypomorphic (Pik3c3Hypo/WT) littermates (Het), which already express a lower level of Pik3c3 than wild-type (Pik3c3WT/WT) littermates (WT). Of note, although an equal amount (10 or 20 μg) of total protein from different tissue homogenates was loaded, both β-actin and α-tubulin (two commonly used loading controls) were expressed at considerably different levels in different tissues, reminiscent to a previous report (28). However, expression levels of either β-actin or α-tubulin in the same type of tissues examined (including spleen, brain, lung, heart, liver, pancreas, testis, and adipose tissue) were comparable among WT, Het, and Homo Pik3c3 hypomorphic mice, validating the comparison of Pik3c3 expression levels as well as the equal loading of total protein. Shown are representative blots from n = 6 mice/genotype group with similar results.
    Figure Legend Snippet: The reduction of Pik3c3 expression also occurs in other organs of the Pik3c3 hypomorphic mouse model. Immunoblot analysis indicated that homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice (Homo) express a markedly lower level of Pik3c3 than heterozygous Pik3c3 hypomorphic (Pik3c3Hypo/WT) littermates (Het), which already express a lower level of Pik3c3 than wild-type (Pik3c3WT/WT) littermates (WT). Of note, although an equal amount (10 or 20 μg) of total protein from different tissue homogenates was loaded, both β-actin and α-tubulin (two commonly used loading controls) were expressed at considerably different levels in different tissues, reminiscent to a previous report (28). However, expression levels of either β-actin or α-tubulin in the same type of tissues examined (including spleen, brain, lung, heart, liver, pancreas, testis, and adipose tissue) were comparable among WT, Het, and Homo Pik3c3 hypomorphic mice, validating the comparison of Pik3c3 expression levels as well as the equal loading of total protein. Shown are representative blots from n = 6 mice/genotype group with similar results.

    Techniques Used: Expressing, Western Blot

    The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice did not cause any apparent phenotype. Pik3c3Hypo/Hypo mice had a mean body weight (A), left kidney weight (B), right kidney weight (C), and left and right kidney-to-body weight ratio (D) that were not statistically different from those of Pik3c3Hypo/WT and Pik3c3WT/WT littermates. Values are means ± SE; n = 7 mice for each group. Furthermore, hematoxylin and eosin staining revealed no difference in renal histology among Pik3c3Hypo/Hypo, Pik3c3Hypo/WT, and Pik3c3WT/WT mice (E). Shown are representative images from n = 6 for each group with similar results.
    Figure Legend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice did not cause any apparent phenotype. Pik3c3Hypo/Hypo mice had a mean body weight (A), left kidney weight (B), right kidney weight (C), and left and right kidney-to-body weight ratio (D) that were not statistically different from those of Pik3c3Hypo/WT and Pik3c3WT/WT littermates. Values are means ± SE; n = 7 mice for each group. Furthermore, hematoxylin and eosin staining revealed no difference in renal histology among Pik3c3Hypo/Hypo, Pik3c3Hypo/WT, and Pik3c3WT/WT mice (E). Shown are representative images from n = 6 for each group with similar results.

    Techniques Used: Expressing, Staining

    Body weight, left kidney weight, left kidney-to-body weight ratio, BUN, and serum creatinine levels in Pik3c3 WT/WT , Pik3c3 Hypo/WT , and Pik3c3 Hypo/Hypo mice 7 days after sham or uninephrectomy surgery
    Figure Legend Snippet: Body weight, left kidney weight, left kidney-to-body weight ratio, BUN, and serum creatinine levels in Pik3c3 WT/WT , Pik3c3 Hypo/WT , and Pik3c3 Hypo/Hypo mice 7 days after sham or uninephrectomy surgery

    Techniques Used:

    The enlargement of proximal tubules and glomeruli induced by unilateral nephrectomy (UNX) was inhibited in Pik3c3 hypomorphic (Hypo) mice. UNX-induced renal glomerular hypertrophy and proximal tubular hypertrophy were assessed by double immunofluorescence staining with synaptopodin to highlight glomeruli in red and Lotus tetragonolobus lectin (LTL) to label renal proximal tubules in green (A). The area of proximal tubules (B) and area of glomeruli (C) were measured as detailed in methods (n = 7 mice/group). Values are means ± SE. P values for the compared groups are indicated. N.S., not statistically significant; WT, wild type.
    Figure Legend Snippet: The enlargement of proximal tubules and glomeruli induced by unilateral nephrectomy (UNX) was inhibited in Pik3c3 hypomorphic (Hypo) mice. UNX-induced renal glomerular hypertrophy and proximal tubular hypertrophy were assessed by double immunofluorescence staining with synaptopodin to highlight glomeruli in red and Lotus tetragonolobus lectin (LTL) to label renal proximal tubules in green (A). The area of proximal tubules (B) and area of glomeruli (C) were measured as detailed in methods (n = 7 mice/group). Values are means ± SE. P values for the compared groups are indicated. N.S., not statistically significant; WT, wild type.

    Techniques Used: Double Immunofluorescence Staining

    The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice inhibits the mammalian target of rapamycin complex 1 signaling pathway. Immunoblot analysis of renal cortical homogenates revealed a graded reduction of unilateral nephrectomy (UNX)-induced ribosomal protein S6 (rpS6) phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (A). Each lane represents one sample from the left kidney of a right uninephrectomized or sham-operated individual mouse. Immunohistochemistry (IHC) staining confirmed the graded inhibition of UNX-induced rpS6 phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (B). Shown are representative blots and images from n = 7 mice/group with similar results.
    Figure Legend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice inhibits the mammalian target of rapamycin complex 1 signaling pathway. Immunoblot analysis of renal cortical homogenates revealed a graded reduction of unilateral nephrectomy (UNX)-induced ribosomal protein S6 (rpS6) phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (A). Each lane represents one sample from the left kidney of a right uninephrectomized or sham-operated individual mouse. Immunohistochemistry (IHC) staining confirmed the graded inhibition of UNX-induced rpS6 phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (B). Shown are representative blots and images from n = 7 mice/group with similar results.

    Techniques Used: Expressing, Western Blot, Immunohistochemistry, Inhibition

    A schematic summary illustrating the main concept of the present work demonstrating that Pik3c3 plays a pivotal role in mediating nephron deficit-induced compensatory nephron hypertrophy (CNH). Specifically, using a mouse model of uninephrectomy (UNX) to induce a 50% nephron reduction, the present study provides the first definitive evidence showing that Pikc3c plays an indispensable role in regulating the degree of CNH by generating Pik3c3 hypomorphic mouse model. Our hypertrophy data indicate that the degree of UNX-induced CNH is tightly regulated by the expression level of Pik3c3. Together with our previous demonstration that phosphorylated ribosomal protein S6 (rpS6) is a downstream effector of the mammalian target of rapamycin complex 1 (mTORC1)-S6 kinase 1 (S6K1) signaling pathway mediating CNH (53), our signaling data examining the level of rpS6 phosphorylation in the present study indicate that Pik3c3 functions upstream of the mTORC1-S6K1-rpS6 pathway mediating increased protein synthesis to drive the development of CNH.
    Figure Legend Snippet: A schematic summary illustrating the main concept of the present work demonstrating that Pik3c3 plays a pivotal role in mediating nephron deficit-induced compensatory nephron hypertrophy (CNH). Specifically, using a mouse model of uninephrectomy (UNX) to induce a 50% nephron reduction, the present study provides the first definitive evidence showing that Pikc3c plays an indispensable role in regulating the degree of CNH by generating Pik3c3 hypomorphic mouse model. Our hypertrophy data indicate that the degree of UNX-induced CNH is tightly regulated by the expression level of Pik3c3. Together with our previous demonstration that phosphorylated ribosomal protein S6 (rpS6) is a downstream effector of the mammalian target of rapamycin complex 1 (mTORC1)-S6 kinase 1 (S6K1) signaling pathway mediating CNH (53), our signaling data examining the level of rpS6 phosphorylation in the present study indicate that Pik3c3 functions upstream of the mTORC1-S6K1-rpS6 pathway mediating increased protein synthesis to drive the development of CNH.

    Techniques Used: Expressing



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    antibodies against pik3c3 z r016  (Echelon Biosciences)
    92
    Echelon Biosciences antibodies against pik3c3 z r016
    Generation of a <t>Pik3c3</t> hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.
    Antibodies Against Pik3c3 Z R016, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against pik3c3 z r016/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against pik3c3 z r016 - by Bioz Stars, 2024-12
    92/100 stars
    		  Buy from Supplier

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    Generation of a Pik3c3 hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy

    doi: 10.1152/ajprenal.00381.2019

    Figure Lengend Snippet: Generation of a Pik3c3 hypomorphic mouse model. Schematic illustration of the wild-type allele, targeting vector, and hypomorphic allele of the mouse Pik3c3 gene, which enabled us to generate homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice and heterozygous Pik3c3-hypomorphic (Pik3c3Hypo/WT) mice to compare with their wild-type (Pik3c3WT/WT) littermates (A). Both immunoblot (B) and immunohistochemistry staining (C) indicated that Pik3c3Hypo/Hypo mice express a markedly lower level of Pik3c3 than Pik3c3Hypo/WT littermates, which already express a lower level of Pik3c3 than Pik3c3WT/WT littermates. E, embroynic day. Shown are representative blots and images from n = 6–8 mice/genotype group with similar results.

    Article Snippet: Antibodies against Pik3c3 (Z-R016) were purchased from Echelon Biosciences (Salt Lake City, UT).

    Techniques: Plasmid Preparation, Western Blot, Immunohistochemistry, Staining

    The reduction of Pik3c3 expression also occurs in other organs of the Pik3c3 hypomorphic mouse model. Immunoblot analysis indicated that homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice (Homo) express a markedly lower level of Pik3c3 than heterozygous Pik3c3 hypomorphic (Pik3c3Hypo/WT) littermates (Het), which already express a lower level of Pik3c3 than wild-type (Pik3c3WT/WT) littermates (WT). Of note, although an equal amount (10 or 20 μg) of total protein from different tissue homogenates was loaded, both β-actin and α-tubulin (two commonly used loading controls) were expressed at considerably different levels in different tissues, reminiscent to a previous report (28). However, expression levels of either β-actin or α-tubulin in the same type of tissues examined (including spleen, brain, lung, heart, liver, pancreas, testis, and adipose tissue) were comparable among WT, Het, and Homo Pik3c3 hypomorphic mice, validating the comparison of Pik3c3 expression levels as well as the equal loading of total protein. Shown are representative blots from n = 6 mice/genotype group with similar results.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy

    doi: 10.1152/ajprenal.00381.2019

    Figure Lengend Snippet: The reduction of Pik3c3 expression also occurs in other organs of the Pik3c3 hypomorphic mouse model. Immunoblot analysis indicated that homozygous Pik3c3 hypomorphic (Pik3c3Hypo/Hypo) mice (Homo) express a markedly lower level of Pik3c3 than heterozygous Pik3c3 hypomorphic (Pik3c3Hypo/WT) littermates (Het), which already express a lower level of Pik3c3 than wild-type (Pik3c3WT/WT) littermates (WT). Of note, although an equal amount (10 or 20 μg) of total protein from different tissue homogenates was loaded, both β-actin and α-tubulin (two commonly used loading controls) were expressed at considerably different levels in different tissues, reminiscent to a previous report (28). However, expression levels of either β-actin or α-tubulin in the same type of tissues examined (including spleen, brain, lung, heart, liver, pancreas, testis, and adipose tissue) were comparable among WT, Het, and Homo Pik3c3 hypomorphic mice, validating the comparison of Pik3c3 expression levels as well as the equal loading of total protein. Shown are representative blots from n = 6 mice/genotype group with similar results.

    Article Snippet: Antibodies against Pik3c3 (Z-R016) were purchased from Echelon Biosciences (Salt Lake City, UT).

    Techniques: Expressing, Western Blot

    The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice did not cause any apparent phenotype. Pik3c3Hypo/Hypo mice had a mean body weight (A), left kidney weight (B), right kidney weight (C), and left and right kidney-to-body weight ratio (D) that were not statistically different from those of Pik3c3Hypo/WT and Pik3c3WT/WT littermates. Values are means ± SE; n = 7 mice for each group. Furthermore, hematoxylin and eosin staining revealed no difference in renal histology among Pik3c3Hypo/Hypo, Pik3c3Hypo/WT, and Pik3c3WT/WT mice (E). Shown are representative images from n = 6 for each group with similar results.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy

    doi: 10.1152/ajprenal.00381.2019

    Figure Lengend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice did not cause any apparent phenotype. Pik3c3Hypo/Hypo mice had a mean body weight (A), left kidney weight (B), right kidney weight (C), and left and right kidney-to-body weight ratio (D) that were not statistically different from those of Pik3c3Hypo/WT and Pik3c3WT/WT littermates. Values are means ± SE; n = 7 mice for each group. Furthermore, hematoxylin and eosin staining revealed no difference in renal histology among Pik3c3Hypo/Hypo, Pik3c3Hypo/WT, and Pik3c3WT/WT mice (E). Shown are representative images from n = 6 for each group with similar results.

    Article Snippet: Antibodies against Pik3c3 (Z-R016) were purchased from Echelon Biosciences (Salt Lake City, UT).

    Techniques: Expressing, Staining

    Body weight, left kidney weight, left kidney-to-body weight ratio, BUN, and serum creatinine levels in Pik3c3 WT/WT , Pik3c3 Hypo/WT , and Pik3c3 Hypo/Hypo mice 7 days after sham or uninephrectomy surgery

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy

    doi: 10.1152/ajprenal.00381.2019

    Figure Lengend Snippet: Body weight, left kidney weight, left kidney-to-body weight ratio, BUN, and serum creatinine levels in Pik3c3 WT/WT , Pik3c3 Hypo/WT , and Pik3c3 Hypo/Hypo mice 7 days after sham or uninephrectomy surgery

    Article Snippet: Antibodies against Pik3c3 (Z-R016) were purchased from Echelon Biosciences (Salt Lake City, UT).

    Techniques:

    The enlargement of proximal tubules and glomeruli induced by unilateral nephrectomy (UNX) was inhibited in Pik3c3 hypomorphic (Hypo) mice. UNX-induced renal glomerular hypertrophy and proximal tubular hypertrophy were assessed by double immunofluorescence staining with synaptopodin to highlight glomeruli in red and Lotus tetragonolobus lectin (LTL) to label renal proximal tubules in green (A). The area of proximal tubules (B) and area of glomeruli (C) were measured as detailed in methods (n = 7 mice/group). Values are means ± SE. P values for the compared groups are indicated. N.S., not statistically significant; WT, wild type.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy

    doi: 10.1152/ajprenal.00381.2019

    Figure Lengend Snippet: The enlargement of proximal tubules and glomeruli induced by unilateral nephrectomy (UNX) was inhibited in Pik3c3 hypomorphic (Hypo) mice. UNX-induced renal glomerular hypertrophy and proximal tubular hypertrophy were assessed by double immunofluorescence staining with synaptopodin to highlight glomeruli in red and Lotus tetragonolobus lectin (LTL) to label renal proximal tubules in green (A). The area of proximal tubules (B) and area of glomeruli (C) were measured as detailed in methods (n = 7 mice/group). Values are means ± SE. P values for the compared groups are indicated. N.S., not statistically significant; WT, wild type.

    Article Snippet: Antibodies against Pik3c3 (Z-R016) were purchased from Echelon Biosciences (Salt Lake City, UT).

    Techniques: Double Immunofluorescence Staining

    The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice inhibits the mammalian target of rapamycin complex 1 signaling pathway. Immunoblot analysis of renal cortical homogenates revealed a graded reduction of unilateral nephrectomy (UNX)-induced ribosomal protein S6 (rpS6) phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (A). Each lane represents one sample from the left kidney of a right uninephrectomized or sham-operated individual mouse. Immunohistochemistry (IHC) staining confirmed the graded inhibition of UNX-induced rpS6 phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (B). Shown are representative blots and images from n = 7 mice/group with similar results.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy

    doi: 10.1152/ajprenal.00381.2019

    Figure Lengend Snippet: The reduction of Pik3c3 expression in Pik3c3 hypomorphic mice inhibits the mammalian target of rapamycin complex 1 signaling pathway. Immunoblot analysis of renal cortical homogenates revealed a graded reduction of unilateral nephrectomy (UNX)-induced ribosomal protein S6 (rpS6) phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (A). Each lane represents one sample from the left kidney of a right uninephrectomized or sham-operated individual mouse. Immunohistochemistry (IHC) staining confirmed the graded inhibition of UNX-induced rpS6 phosphorylation in Pik3c3Hypo/WT and Pik3c3Hypo/Hypo mice compared with Pik3c3WT/WT mice (B). Shown are representative blots and images from n = 7 mice/group with similar results.

    Article Snippet: Antibodies against Pik3c3 (Z-R016) were purchased from Echelon Biosciences (Salt Lake City, UT).

    Techniques: Expressing, Western Blot, Immunohistochemistry, Inhibition

    A schematic summary illustrating the main concept of the present work demonstrating that Pik3c3 plays a pivotal role in mediating nephron deficit-induced compensatory nephron hypertrophy (CNH). Specifically, using a mouse model of uninephrectomy (UNX) to induce a 50% nephron reduction, the present study provides the first definitive evidence showing that Pikc3c plays an indispensable role in regulating the degree of CNH by generating Pik3c3 hypomorphic mouse model. Our hypertrophy data indicate that the degree of UNX-induced CNH is tightly regulated by the expression level of Pik3c3. Together with our previous demonstration that phosphorylated ribosomal protein S6 (rpS6) is a downstream effector of the mammalian target of rapamycin complex 1 (mTORC1)-S6 kinase 1 (S6K1) signaling pathway mediating CNH (53), our signaling data examining the level of rpS6 phosphorylation in the present study indicate that Pik3c3 functions upstream of the mTORC1-S6K1-rpS6 pathway mediating increased protein synthesis to drive the development of CNH.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: The expression level of class III phosphatidylinositol-3 kinase controls the degree of compensatory nephron hypertrophy

    doi: 10.1152/ajprenal.00381.2019

    Figure Lengend Snippet: A schematic summary illustrating the main concept of the present work demonstrating that Pik3c3 plays a pivotal role in mediating nephron deficit-induced compensatory nephron hypertrophy (CNH). Specifically, using a mouse model of uninephrectomy (UNX) to induce a 50% nephron reduction, the present study provides the first definitive evidence showing that Pikc3c plays an indispensable role in regulating the degree of CNH by generating Pik3c3 hypomorphic mouse model. Our hypertrophy data indicate that the degree of UNX-induced CNH is tightly regulated by the expression level of Pik3c3. Together with our previous demonstration that phosphorylated ribosomal protein S6 (rpS6) is a downstream effector of the mammalian target of rapamycin complex 1 (mTORC1)-S6 kinase 1 (S6K1) signaling pathway mediating CNH (53), our signaling data examining the level of rpS6 phosphorylation in the present study indicate that Pik3c3 functions upstream of the mTORC1-S6K1-rpS6 pathway mediating increased protein synthesis to drive the development of CNH.

    Article Snippet: Antibodies against Pik3c3 (Z-R016) were purchased from Echelon Biosciences (Salt Lake City, UT).

    Techniques: Expressing