Journal: Frontiers in Pharmacology
Article Title: Puerarin Relieves Paclitaxel-Induced Neuropathic Pain: The Role of Nav1.8 β1 Subunit of Sensory Neurons
Figure Lengend Snippet: Puerarin inhibits Na v 1.8 in a neuropathic pain state may by blocking β1 subunit in DRG neurons (Ctrl, control; PTX, paclitaxel; Pue, puerarin). (A) Co-IP showed that paclitaxel treatment significantly increased the binding between Na v 1.8 and β1. n = 6 rats in each group. ∗∗∗ P
Article Snippet: The membranes were placed in blocking buffer for 1 h at room temperature and incubated in a primary antibody against Nav β1 (1:400, Alomone Labs, Israel), Nav 1.8 (1:200, Alomone Labs, Israel), or β-actin (1:2000, CST, United States) overnight at 4°C.
Techniques: Blocking Assay, Co-Immunoprecipitation Assay, Binding Assay