Journal: Pharmaceutical Biology

Article Title: Cryptotanshinone enhances the efficacy of Bcr-Abl tyrosine kinase inhibitors via inhibiting STAT3 and eIF4E signalling pathways in chronic myeloid leukaemia

doi: 10.1080/13880209.2021.1944224

Figure Lengend Snippet: The effect of combined imatinib and CPT on the expression levels of Ki-67 and PCNA in CML xenograft tissues. (A) The expression levels of Ki-67 and PCNA were determined by immunohistochemical staining. (B) Semi-quantitative analysis of the immunohistochemical staining of Ki-67 after the treatment of imatinib and CPT. (C) Semi-quantitative analysis of the immunohistochemical staining of PCNA after the treatment of imatinib and CPT. Scale bar = 50 µm. Average data were represented as the mean ± SD ( n = 5). * p < 0.05, ** p < 0.01 and *** p < 0.001 denote significant differences compared with the control group.

Article Snippet: The rabbit polyclonal antibody against Ki-67 (PB9026) and mouse monoclonal antibody against PCNA (BM0104) was obtained from Boster Biological Technology Co., Ltd.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: OncoTargets and therapy

Article Title: Aloperine executes antitumor effects through the induction of apoptosis and cell cycle arrest in prostate cancer in vitro and in vivo

doi: 10.2147/OTT.S165262

Figure Lengend Snippet: Aloperine suppressed PC3 xenograft tumor growth in vivo. ( A and B ) PC3 cells were injected into the right flank of 6-week-old male, nude mice. Subcutaneous tumors were formed in nude mice after treatment with either PBS or aloperine (30 mg/kg) for 28 days. ( C ) Tumor volume of aloperine-treated PC3 cells at the indicated time ( *P <0.05 vs vehicle, n=5). ( D ) Histograms describing the mean tumor weights of each group ( *P <0.05 vs vehicle, n=5). ( E ) Mice weight at the indicated time. ( F ) Immunohistochemical staining of Ki-67 and TUNEL assay in the endpoint tumors. ( G and H ) Quantitative analysis of Ki-67-positive and TUNEL-positive cells in tumor tissues of different groups ( *P <0.05 vs vehicle, n=5). Abbreviation: TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling.

Article Snippet: The tumor tissues (both treatment and control group) were stained with antibody against Ki-67 (1:100; Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) to evaluate the proliferation rate of PCa cells in vivo.

Techniques: In Vivo, Injection, Immunohistochemical staining, Staining, TUNEL Assay, End Labeling

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis

doi: 10.4143/crt.2018.544

Figure Lengend Snippet: Poly(A)-binding protein-interacting protein 1 (Paip1) attenuated the ability of proliferation of cervical cancer (CC) cells in vitro . (A) The protein expression of Paip1 in CC cells was examined by western blot analysis. Actin was used as a loading control. (B) The protein expression of Paip1 in the constructed CC cells was confirmed by western blot analysis. Actin was used as a loading control. (C, D) Cell proliferation was examined by MTT (C) and colony formation (D) in the constructed cells. Data are represented as mean±standard error of mean of at least three independent experiments, **p < 0.01.

Article Snippet: Antibody against Ki-67 was purchased from BOSTER (Wuhan, China).

Techniques: Binding Assay, In Vitro, Expressing, Western Blot, Construct

Journal: Cancer Research and Treatment : Official Journal of Korean Cancer Association

Article Title: Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis

doi: 10.4143/crt.2018.544

Figure Lengend Snippet: Poly(A)-binding protein-interacting protein 1 (Paip1) promoted tumor growth in vivo . (A, B) Indicated cells were injected into the nude mice, and statistical comparison of the differences in tumor weight was shown. (C, D) Expression levels of Ki-67, Bcl-2, and Bax in the xenograft tumors tissues were detected by immunohistochemistry.

Article Snippet: Antibody against Ki-67 was purchased from BOSTER (Wuhan, China).

Techniques: Binding Assay, In Vivo, Injection, Expressing, Immunohistochemistry

Journal: Frontiers in Pharmacology

Article Title: Saikosaponin A Inhibits Breast Cancer by Regulating Th1/Th2 Balance

doi: 10.3389/fphar.2019.00624

Figure Lengend Snippet: The effects of SSa on tumor cell proliferation and infiltrated T lymphocytes in tumors. (A) Immunohistochemistry staining of Ki-67, CD8, and CD4 in rat tumors. (B) Percentage of Ki-67-positive cells. (C) Number of CD4+ or CD8+ cells. Data were expressed as mean ± SD ( n = 12). * P < 0.05, ** P < 0.01 vs. control group.

Article Snippet: Briefly, sections were incubated with primary antibodies against Ki-67 (1:200, Boster, Wuhan, China), CD8 (1:200, Santa Cruz, CA, USA), CD4 (1:200, Santa Cruz, CA, USA), IL-12 (1:400, Santa Cruz, CA, USA), IL-12 receptor (IL-12R, 1:400, Santa Cruz, CA, USA), or pSTAT4 (1:200, Santa Cruz, CA, USA), respectively.

Techniques: Immunohistochemistry, Staining