antibodies against ki 67  (Boster Bio)

Journal: Cell Death & Disease

Article Title: Tumor-suppressive function of long noncoding RNA MALAT1 in glioma cells by downregulation of MMP2 and inactivation of ERK/MAPK signaling

doi: 10.1038/cddis.2015.407

Figure Lengend Snippet: Effects of upregulation and downregulation of MALAT1 on U87 and U251 cell proliferation. ( a and b ) Cellular proliferation of untransfected or transfected U87 and U251 cells was measured using a CCK-8 assay daily for 3 days. ( c ) Cellular proliferation of untransfected or transfected U87 and U251 cells was measured by testing the expression of Ki-67. ( d and e ) The percentage of Ki-67-positive cells was calculated. Results are expressed as mean±S.D. from three independent experiments ( P

Article Snippet: Slides were incubated with primary antibodies against Ki-67 (Boster Bioengineering Co., Wuhan, China), and with antibodies against MMP2 and TIMP3 (Abcam).

Techniques: Transfection, CCK-8 Assay, Expressing

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Involvement of aberrantly activated HOTAIR/EZH2/miR-193a feedback loop in progression of prostate cancer

doi: 10.1186/s13046-017-0629-7

Figure Lengend Snippet: Overexpression of miR-193a suppresses the formation of prostate xenograft tumors in vivo. a Fluorescence microscope is used for detecting transfection efficiency for LV-miR-193a transfection. b and c Subcutaneous tumors formed in nude mice by PC3 cells with stably overexpression of miR-193a or control at 28 days. d Tumor volume of miR-193a-overexpressiong PC3 cells at indicated time. e Histograms describing the mean tumor weights of each group. f Immunohistochemical staining of Ki-67, CD31, and CD34 in the endpoint tumors. Scale bars represent 50 μm. Each bar represents the mean ± SD of three independent experiments. * P

Article Snippet: Thereafter tissues were stained with antibody against Ki-67, CD31 and CD34 (all 1:100, Boster, Wuhan, China) according to the manufacturer’s instructions.

Techniques: Over Expression, In Vivo, Fluorescence, Microscopy, Transfection, Mouse Assay, Stable Transfection, Immunohistochemistry, Staining

Journal: Oncotarget

Article Title: WX-132-18B, a novel microtubule inhibitor, exhibits promising anti-tumor effects

doi: 10.18632/oncotarget.17710

Figure Lengend Snippet: WX-132-18B inhibited tumor growth in vivo (A) Effects of tumor growth inhibition of WX-132-18B in S180 xenograft KM mice, H460 xenograft and BGC-823 xenograft Nu/Nu nude mice. Mice were treated with taxol or different doses (0.25, 0.5 and 1.0 mg/kg) of WX-132-18B for three weeks. Tumor volumes were measured before treatment and at the indicated days of treatment. At the end of the experiment, tumors were resected and weighed. Values are means±SD of tumor volume or weight for all the tested mice in each group. (B) Histological examination and immunohistochemical analysis of the expression and distribution of CD31, caspase 3, and Ki67 in BGC-823 xenograft tumor. The red arrow indicates blood vessels. Scale bar, 100 μm.

Article Snippet: Rabbit antibodies against Ki67, Caspase 3, and CD31, horseradish peroxidase-labeled goat anti-rabbit secondary antibodies, the diaminobenzidine (DAB) substrate kits, and the HE staining kit were purchased from Wuhan Boster Biological Technology, Ltd (Wuhan, China).

Techniques: In Vivo, Inhibition, Mouse Assay, Immunohistochemistry, Expressing

Journal: Frontiers in Pharmacology

Article Title: Antitumor Effect of Simvastatin in Combination With DNA Methyltransferase Inhibitor on Gastric Cancer via GSDME-Mediated Pyroptosis

doi: 10.3389/fphar.2022.860546

Figure Lengend Snippet: 5-Aza-CdR promotes SIM-induced GC pyroptosis via caspase 3/GSDME pathway in vivo . (A) TUNEL, H E staining and Ki67 IHC of xenograft tumour slices after indicated treatment. TUNEL scale bar: 20 µm. White arrows: TUNEL positive cells; H E scale bar: 100 µm. Black arrows: the areas of necrosis; IHC scale bar: 20 µm. Red arrows: Ki67-positive cells. (B) HE staining of heart, liver, spleen, lung, and kidney tissues in each group; scale bar: 100 µm. (C) Schematic diagram to illustrate the mechanisms underlying the induction of pyroptosis in GC by a combined treatment of SIM with 5-Aza-CdR via caspase-3/GSDME activation.

Article Snippet: IHC was performed using antibodies against Ki67, GSDME, and cleaved (Cl)-caspase-3 using an immunohistochemical test kit (Boster, SA1020).

Techniques: In Vivo, TUNEL Assay, Staining, Immunohistochemistry, Activation Assay

Journal: Frontiers in Pharmacology

Article Title: Antitumor Effect of Simvastatin in Combination With DNA Methyltransferase Inhibitor on Gastric Cancer via GSDME-Mediated Pyroptosis

doi: 10.3389/fphar.2022.860546

Figure Lengend Snippet: SIM combined with overexpression-GSDME promotes the progression of GC pyroptosis in vivo . (A) TUNEL and H E staining images for tumour slices after indicated treatment. White arrows indicate TUNEL positive cells. TUNEL scale bar: 20 μm; the areas of necrosis were indicated by black arrows in the figures. H E scale bar: 100 µm. (B) Protein cleavage of GSDME and caspase-3 in tumour tissues, detected by Western blotting (C) Immunohistochemistry (IHC) images of Ki67, Cl-casp3, GSDME and GSDME-N for tumour tissues after indicated treatment; black arrows indicate the IHC staining-positive cells; scale bar: 20 µm. (D) HE staining of heart, liver, spleen, lung, and kidney tissues in each group; scale bar = 100 µm.

Article Snippet: IHC was performed using antibodies against Ki67, GSDME, and cleaved (Cl)-caspase-3 using an immunohistochemical test kit (Boster, SA1020).

Techniques: Over Expression, In Vivo, TUNEL Assay, Staining, Western Blot, Immunohistochemistry