antibodies against histone h3 lysine 27 acetylation  (Cell Signaling Technology Inc)


Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Cell Signaling Technology Inc antibodies against histone h3 lysine 27 acetylation
    Visualization of histone modifications by chromatin immunoprecipitation and DNA sequencing (ChIP-seq) in the human transferrin genomic region. ChIP-seq data was obtained from explants from 5 control livers and 7 livers of patients with severe non-responder alcoholic hepatitis. The genomic region around the transferrin promoter was visualized through the Integrative Genome Viewer (IGV). Histone 3 lysine 4 (H3K4) mono- and tri-methylation as well as H3K27 acetylation and tri-methylation are shown. The decrease in <t>H3K27ac</t> signal indicated the lack of activity in transferrin enhancer and promoter regions, while the decrease of H3K4me3 and increase of H3K27me3 suggested the absence of activity and repressive chromatin configuration, respectively, in the region around the transcription start site (TSS). The light gray areas indicate the regions where these changes were seen (cohort ii)
    Antibodies Against Histone H3 Lysine 27 Acetylation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against histone h3 lysine 27 acetylation/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against histone h3 lysine 27 acetylation - by Bioz Stars, 2023-12
    96/100 stars

    Images

    1) Product Images from "Serum transferrin as a biomarker of hepatocyte nuclear factor 4 alpha activity and hepatocyte function in liver diseases"

    Article Title: Serum transferrin as a biomarker of hepatocyte nuclear factor 4 alpha activity and hepatocyte function in liver diseases

    Journal: BMC Medicine

    doi: 10.1186/s12916-021-01917-6

    Visualization of histone modifications by chromatin immunoprecipitation and DNA sequencing (ChIP-seq) in the human transferrin genomic region. ChIP-seq data was obtained from explants from 5 control livers and 7 livers of patients with severe non-responder alcoholic hepatitis. The genomic region around the transferrin promoter was visualized through the Integrative Genome Viewer (IGV). Histone 3 lysine 4 (H3K4) mono- and tri-methylation as well as H3K27 acetylation and tri-methylation are shown. The decrease in H3K27ac signal indicated the lack of activity in transferrin enhancer and promoter regions, while the decrease of H3K4me3 and increase of H3K27me3 suggested the absence of activity and repressive chromatin configuration, respectively, in the region around the transcription start site (TSS). The light gray areas indicate the regions where these changes were seen (cohort ii)
    Figure Legend Snippet: Visualization of histone modifications by chromatin immunoprecipitation and DNA sequencing (ChIP-seq) in the human transferrin genomic region. ChIP-seq data was obtained from explants from 5 control livers and 7 livers of patients with severe non-responder alcoholic hepatitis. The genomic region around the transferrin promoter was visualized through the Integrative Genome Viewer (IGV). Histone 3 lysine 4 (H3K4) mono- and tri-methylation as well as H3K27 acetylation and tri-methylation are shown. The decrease in H3K27ac signal indicated the lack of activity in transferrin enhancer and promoter regions, while the decrease of H3K4me3 and increase of H3K27me3 suggested the absence of activity and repressive chromatin configuration, respectively, in the region around the transcription start site (TSS). The light gray areas indicate the regions where these changes were seen (cohort ii)

    Techniques Used: Chromatin Immunoprecipitation, DNA Sequencing, ChIP-sequencing, Methylation, Activity Assay

    antibodies against histone h3 lysine 27 acetylation  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc antibodies against histone h3 lysine 27 acetylation
    ChIP-seq shows decreased <t>H3K27Ac</t> and H3K4me1 in HNF4 α -P1 and its targets and enhanced binding of H3K27Ac to HNF4 α P2 promoter. a Data were obtained from ChIP-seq of Human Liver samples from normal ( n = 5) and AH ( n = 6) livers. Antibodies agains Histone 3 <t>Lysine</t> <t>27</t> acetylation (H3K27Ac), Histone 3 Lysine 4 mono and trimethylation (H3K4me1 and H3K4me3) and Histone 3 Lysine 27 trimethylation (H3K27me3) were used in the immunoprecipitation. Integrated Genome Viewer was used to visualize BigWig peak data. b – d Genomic view of sequencing reads present in loci of b HNF4 α targets PCK1 , CYP3A4 and F7 , c Non-HNF4 α target ICAM-1 and d HNF4A . e – g Box plot of fold changes (IP to Input) of all peaks called around the TSS of e HNF4A isoforms P1 and P2, f HNF4A targets PCK1 , CYP3A4 and F7 and g ICAM1 . Significance was determined by two-tailed Student t test in e , f , and g : * P < 0.05, ** P < 0.01, *** P < 0.001. For box-and-whisker plots in e, f, g : perimeters, 25th–75th percentile; midline, median
    Antibodies Against Histone H3 Lysine 27 Acetylation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against histone h3 lysine 27 acetylation/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against histone h3 lysine 27 acetylation - by Bioz Stars, 2023-12
    96/100 stars

    Images

    1) Product Images from "Defective HNF4alpha-dependent gene expression as a driver of hepatocellular failure in alcoholic hepatitis"

    Article Title: Defective HNF4alpha-dependent gene expression as a driver of hepatocellular failure in alcoholic hepatitis

    Journal: Nature Communications

    doi: 10.1038/s41467-019-11004-3

    ChIP-seq shows decreased H3K27Ac and H3K4me1 in HNF4 α -P1 and its targets and enhanced binding of H3K27Ac to HNF4 α P2 promoter. a Data were obtained from ChIP-seq of Human Liver samples from normal ( n = 5) and AH ( n = 6) livers. Antibodies agains Histone 3 Lysine 27 acetylation (H3K27Ac), Histone 3 Lysine 4 mono and trimethylation (H3K4me1 and H3K4me3) and Histone 3 Lysine 27 trimethylation (H3K27me3) were used in the immunoprecipitation. Integrated Genome Viewer was used to visualize BigWig peak data. b – d Genomic view of sequencing reads present in loci of b HNF4 α targets PCK1 , CYP3A4 and F7 , c Non-HNF4 α target ICAM-1 and d HNF4A . e – g Box plot of fold changes (IP to Input) of all peaks called around the TSS of e HNF4A isoforms P1 and P2, f HNF4A targets PCK1 , CYP3A4 and F7 and g ICAM1 . Significance was determined by two-tailed Student t test in e , f , and g : * P < 0.05, ** P < 0.01, *** P < 0.001. For box-and-whisker plots in e, f, g : perimeters, 25th–75th percentile; midline, median
    Figure Legend Snippet: ChIP-seq shows decreased H3K27Ac and H3K4me1 in HNF4 α -P1 and its targets and enhanced binding of H3K27Ac to HNF4 α P2 promoter. a Data were obtained from ChIP-seq of Human Liver samples from normal ( n = 5) and AH ( n = 6) livers. Antibodies agains Histone 3 Lysine 27 acetylation (H3K27Ac), Histone 3 Lysine 4 mono and trimethylation (H3K4me1 and H3K4me3) and Histone 3 Lysine 27 trimethylation (H3K27me3) were used in the immunoprecipitation. Integrated Genome Viewer was used to visualize BigWig peak data. b – d Genomic view of sequencing reads present in loci of b HNF4 α targets PCK1 , CYP3A4 and F7 , c Non-HNF4 α target ICAM-1 and d HNF4A . e – g Box plot of fold changes (IP to Input) of all peaks called around the TSS of e HNF4A isoforms P1 and P2, f HNF4A targets PCK1 , CYP3A4 and F7 and g ICAM1 . Significance was determined by two-tailed Student t test in e , f , and g : * P < 0.05, ** P < 0.01, *** P < 0.001. For box-and-whisker plots in e, f, g : perimeters, 25th–75th percentile; midline, median

    Techniques Used: ChIP-sequencing, Binding Assay, Immunoprecipitation, Sequencing, Two Tailed Test, Whisker Assay

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    Cell Signaling Technology Inc antibodies against histone h3 lysine 27 acetylation
    Visualization of histone modifications by chromatin immunoprecipitation and DNA sequencing (ChIP-seq) in the human transferrin genomic region. ChIP-seq data was obtained from explants from 5 control livers and 7 livers of patients with severe non-responder alcoholic hepatitis. The genomic region around the transferrin promoter was visualized through the Integrative Genome Viewer (IGV). Histone 3 lysine 4 (H3K4) mono- and tri-methylation as well as H3K27 acetylation and tri-methylation are shown. The decrease in <t>H3K27ac</t> signal indicated the lack of activity in transferrin enhancer and promoter regions, while the decrease of H3K4me3 and increase of H3K27me3 suggested the absence of activity and repressive chromatin configuration, respectively, in the region around the transcription start site (TSS). The light gray areas indicate the regions where these changes were seen (cohort ii)
    Antibodies Against Histone H3 Lysine 27 Acetylation, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against histone h3 lysine 27 acetylation/product/Cell Signaling Technology Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against histone h3 lysine 27 acetylation - by Bioz Stars, 2023-12
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Visualization of histone modifications by chromatin immunoprecipitation and DNA sequencing (ChIP-seq) in the human transferrin genomic region. ChIP-seq data was obtained from explants from 5 control livers and 7 livers of patients with severe non-responder alcoholic hepatitis. The genomic region around the transferrin promoter was visualized through the Integrative Genome Viewer (IGV). Histone 3 lysine 4 (H3K4) mono- and tri-methylation as well as H3K27 acetylation and tri-methylation are shown. The decrease in H3K27ac signal indicated the lack of activity in transferrin enhancer and promoter regions, while the decrease of H3K4me3 and increase of H3K27me3 suggested the absence of activity and repressive chromatin configuration, respectively, in the region around the transcription start site (TSS). The light gray areas indicate the regions where these changes were seen (cohort ii)

    Journal: BMC Medicine

    Article Title: Serum transferrin as a biomarker of hepatocyte nuclear factor 4 alpha activity and hepatocyte function in liver diseases

    doi: 10.1186/s12916-021-01917-6

    Figure Lengend Snippet: Visualization of histone modifications by chromatin immunoprecipitation and DNA sequencing (ChIP-seq) in the human transferrin genomic region. ChIP-seq data was obtained from explants from 5 control livers and 7 livers of patients with severe non-responder alcoholic hepatitis. The genomic region around the transferrin promoter was visualized through the Integrative Genome Viewer (IGV). Histone 3 lysine 4 (H3K4) mono- and tri-methylation as well as H3K27 acetylation and tri-methylation are shown. The decrease in H3K27ac signal indicated the lack of activity in transferrin enhancer and promoter regions, while the decrease of H3K4me3 and increase of H3K27me3 suggested the absence of activity and repressive chromatin configuration, respectively, in the region around the transcription start site (TSS). The light gray areas indicate the regions where these changes were seen (cohort ii)

    Article Snippet: It was carried out for four histone modifications, using antibodies against histone H3 Lysine 27 acetylation (H3K27ac, Cell Signaling #8173), histone H3 Lysine 27 tri-methylation (H3K27me3, Cell Signaling #9733), histone H3 Lysine 4 mono-methylation (H3K4me1, EDL, Mayo Clinic, Lot#1), and histone H3 Lysine 4 tri-methylation (H3K4me3, EDL, Mayo Clinic, Lot#1).

    Techniques: Chromatin Immunoprecipitation, DNA Sequencing, ChIP-sequencing, Methylation, Activity Assay