Journal: BMC Cancer

Article Title: EMMPRIN expression is associated with metastatic progression in osteosarcoma

doi: 10.1186/s12885-021-08774-9

Figure Lengend Snippet: Decrease in invasion activities of SaOS-2 cells transfected by EMMPRIN siRNA. Matrigel invasion assays after transfection of the EMMRIN siRNA into SaOS-2 cells for 24 h. Following incubation, the membranes were removed from the inserts and mounted on slides. a. The number of invading cells was counted under a light microscope. The Matrigel assay was performed in triplicates. * p < 0.05 compared with cells transfected with control siRNA. b. Representative images of Matrigel invasion assay. Scale bar, 100 μm

Article Snippet: The membranes were probed with primary antibodies against EMMPRIN (Cell Signaling, #13287S, Lot1) after incubating in a blocking buffer.

Techniques: Transfection, Incubation, Light Microscopy, Matrigel Assay, Invasion Assay

Journal: BMC Cancer

Article Title: EMMPRIN expression is associated with metastatic progression in osteosarcoma

doi: 10.1186/s12885-021-08774-9

Figure Lengend Snippet: EMMPRIN silencing inhibits the metastatic nodes in the lungs. a Metastasis assay by tail vein injection of shRNA transfected 143B cells in BALB/c mice. Lungs were excised for examination at 8 weeks post-injection. The number of lung metastatic nodes was assessed by Bouin’s stain. Arrows indicate visible lung surface metastases. b Tumor volume and c Number of nodules at the study end point. d 143B cells were transfected with mock shRNA and EMMPRIN shRNA for 48 h, and cell lysates were analyzed by western blot using anti-EMMPRIN antibody. β-actin was used as a loading control. e Microscope images by H&E staining show that EMMPRIN silencing significantly inhibits the lung metastatic nodes after inoculation with 143B, 2 × 10 5 cells/ 200 ul. *, p < 0.05 compared with cells transfected with control siRNA. Scale bar, 100 μm. Original magnification, × 10 and × 40

Article Snippet: The membranes were probed with primary antibodies against EMMPRIN (Cell Signaling, #13287S, Lot1) after incubating in a blocking buffer.

Techniques: Injection, shRNA, Transfection, Staining, Western Blot, Microscopy

Journal: Experimental and Therapeutic Medicine

Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

doi: 10.3892/etm.2020.9058

Figure Lengend Snippet: mRNA and protein expression levels of CD147 in LNCaP cells depleted of CD147 as detected by reverse transcription-quantitative polymerase chain reaction and western blotting. (A) mRNA and (B) protein expression in LNCaP/Scramble and LNCaP/shCD147 cells. Values are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation, shRNA

Journal: Experimental and Therapeutic Medicine

Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

doi: 10.3892/etm.2020.9058

Figure Lengend Snippet: CD147 promotes LNCaP cell viability. LNCaP/shCD147 and LNCaP/Scramble cells were seeded in 96-well plates and incubated for 2, 4, 6 or 8 days. Cell viability was determined using a Cell Counting Kit-8 assay. Values are normalized to LNCaP/Scramble and presented as the mean ± standard deviation of three experiments. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

Techniques: Incubation, Cell Counting, Standard Deviation, shRNA

Journal: Experimental and Therapeutic Medicine

Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

doi: 10.3892/etm.2020.9058

Figure Lengend Snippet: CD147 promotes the migration and invasion of LNCaP cells. Effect of CD147 knockdown on (A) migration and (B) invasion of LNCaP cells. Scale bars, 20 µm. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

Techniques: Migration, Standard Deviation, shRNA

Journal: Experimental and Therapeutic Medicine

Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

doi: 10.3892/etm.2020.9058

Figure Lengend Snippet: CD147 promotes epithelial-mesenchymal transition of LNCaP cells. The expression of the epithelial marker E-cadherin and the mesenchymal markers N-cadherin and vimentin was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values shown are presented as the mean ± standard deviation of three experiments. ** P<0.01 vs. LNCaP/Scramble. LNCaP, lymph node carcinoma of the prostate; sh, short hairpin RNA.

Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

Techniques: Expressing, Marker, Western Blot, Standard Deviation, shRNA

Journal: Experimental and Therapeutic Medicine

Article Title: CD147 promotes epithelial-mesenchymal transition of prostate cancer cells via the Wnt/β-catenin pathway

doi: 10.3892/etm.2020.9058

Figure Lengend Snippet: CD147 regulates epithelial-mesenchymal transition via the Wnt/β-catenin pathway. (A and B) Expression of β-catenin, Snail and p-GSK-3β (Ser9) was determined in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. (C and D) Expression of β-catenin, Snail, p-β-catenin (Ser33/37/Thr41), vimentin and E-cadherin following treatment with or without LiCl in LNCaP/Scramble and LNCaP/shCD147 cells by western blotting. Values are presented as the mean ± standard deviation of three experiments. Lanes 1 and 3, LNCaP/Scramble group; Lanes 2 and 4, LNCaP/shCD147 group. * P<0.05, ** P<0.01 vs. LNCaP/Scramble; # P<0.05, ## P<0.01 vs. LNCaP/shCD147. LNCaP, lymph node carcinoma of the prostate; LiCl, lithium chloride; sh, short hairpin RNA; p-, phosphorylated; GSK-3β, glycogen synthase kinase-3β.

Article Snippet: The membranes were then blocked with 5% nonfat milk in PBS containing 0.05% Tween-20 at room temperature for 2 h. The membranes were incubated with rabbit monoclonal antibodies against CD147 (cat. no. 13287), human p-glycogen synthase kinase (GSK)-3β (Ser 9; cat. no. 9322), GSK-3β (cat. no. 9315), E-cadherin (cat. no. 3195), N-cadherin (cat. no. 13116), vimentin (cat. no. 5741), β-catenin (cat. no. 8480), p-β-catenin (Ser 33/37/Thr 41; cat. no. 9561) and Snail (cat. no. 3879) all at 1:1,000 dilution (Cell Signaling Technology, Inc.) overnight at 4˚C. β-actin (cat. no. 4970) and lamin B (cat. no. 13435) were used as loading controls (both 1:2,000; Cell Signaling Technology, Inc.).

Techniques: Expressing, Western Blot, Standard Deviation, shRNA