rabbit antibodies against cyclin d1  (Danaher Inc)


Bioz Verified Symbol Danaher Inc is a verified supplier
Bioz Manufacturer Symbol Danaher Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Danaher Inc rabbit antibodies against cyclin d1
    Rabbit Antibodies Against Cyclin D1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antibodies against cyclin d1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    rabbit antibodies against cyclin d1  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
    Bioz Manufacturer Symbol Danaher Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Danaher Inc rabbit antibodies against cyclin d1
    PPV-6 downregulates induction of the cell cycle/apoptosis markers and decreases the number of post-mitotic neurons incorporating BrdU upon Aβ25-35 exposure. ( A – D ) Primary cortical cultures were exposed to Aβ25-35 (10 μM) with or without PPV-6 (250 μg/mL) for 8 h before detection of <t>cyclin</t> <t>D1</t> ( A ) or 24 h before detection of PCNA ( B ), p-Histone H3 ( C ), as well as pro- and cleaved caspase-3 ( D ). ( E – G ) Cortical neurons treated with Aβ25-35 (10 μM), PPV-6 (250 μg/mL), or both for 24 h were subjected to immunostaining with antibodies against various cell cycle markers ( green ), including cyclin D1 ( E ), PCNA ( F ), and p-Histone H3 ( G ); a MAP-2 ( red ) antibody stained the mature neurons. ( H ) The cultures were stained with the antibodies against BrdU ( green ) and neuronal marker NeuN ( red ); Hoechst 33258 ( blue ) served as counterstaining. White arrows denote the mature neurons expressing cell cycle markers or incorporating BrdU. Scale bar = 10 μm. Mean ± SEM from N = 3 in ( A – D ), N = 6 in ( E – G ), and N = 3 in ( H ). Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. * and # denote p < 0.05.
    Rabbit Antibodies Against Cyclin D1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antibodies against cyclin d1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Polysaccharides from Basella alba Protect Post-Mitotic Neurons against Cell Cycle Re-Entry and Apoptosis Induced by the Amyloid-Beta Peptide by Blocking Sonic Hedgehog Expression"

    Article Title: Polysaccharides from Basella alba Protect Post-Mitotic Neurons against Cell Cycle Re-Entry and Apoptosis Induced by the Amyloid-Beta Peptide by Blocking Sonic Hedgehog Expression

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25137316

    PPV-6 downregulates induction of the cell cycle/apoptosis markers and decreases the number of post-mitotic neurons incorporating BrdU upon Aβ25-35 exposure. ( A – D ) Primary cortical cultures were exposed to Aβ25-35 (10 μM) with or without PPV-6 (250 μg/mL) for 8 h before detection of cyclin D1 ( A ) or 24 h before detection of PCNA ( B ), p-Histone H3 ( C ), as well as pro- and cleaved caspase-3 ( D ). ( E – G ) Cortical neurons treated with Aβ25-35 (10 μM), PPV-6 (250 μg/mL), or both for 24 h were subjected to immunostaining with antibodies against various cell cycle markers ( green ), including cyclin D1 ( E ), PCNA ( F ), and p-Histone H3 ( G ); a MAP-2 ( red ) antibody stained the mature neurons. ( H ) The cultures were stained with the antibodies against BrdU ( green ) and neuronal marker NeuN ( red ); Hoechst 33258 ( blue ) served as counterstaining. White arrows denote the mature neurons expressing cell cycle markers or incorporating BrdU. Scale bar = 10 μm. Mean ± SEM from N = 3 in ( A – D ), N = 6 in ( E – G ), and N = 3 in ( H ). Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. * and # denote p < 0.05.
    Figure Legend Snippet: PPV-6 downregulates induction of the cell cycle/apoptosis markers and decreases the number of post-mitotic neurons incorporating BrdU upon Aβ25-35 exposure. ( A – D ) Primary cortical cultures were exposed to Aβ25-35 (10 μM) with or without PPV-6 (250 μg/mL) for 8 h before detection of cyclin D1 ( A ) or 24 h before detection of PCNA ( B ), p-Histone H3 ( C ), as well as pro- and cleaved caspase-3 ( D ). ( E – G ) Cortical neurons treated with Aβ25-35 (10 μM), PPV-6 (250 μg/mL), or both for 24 h were subjected to immunostaining with antibodies against various cell cycle markers ( green ), including cyclin D1 ( E ), PCNA ( F ), and p-Histone H3 ( G ); a MAP-2 ( red ) antibody stained the mature neurons. ( H ) The cultures were stained with the antibodies against BrdU ( green ) and neuronal marker NeuN ( red ); Hoechst 33258 ( blue ) served as counterstaining. White arrows denote the mature neurons expressing cell cycle markers or incorporating BrdU. Scale bar = 10 μm. Mean ± SEM from N = 3 in ( A – D ), N = 6 in ( E – G ), and N = 3 in ( H ). Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. * and # denote p < 0.05.

    Techniques Used: Immunostaining, Staining, Marker, Expressing

    PPV-6 downregulates expression of the cell cycle/apoptosis markers as well as BrdU incorporation induced by Aβ1-42. ( A – D ) Primary cortical cultures were exposed to Aβ1-42 (5 μM), PPV-6 (250 μg/mL), or both for 48 h before detection of cyclin D1 ( A ), PCNA ( B ), p-Histone H3 ( C ), as well as pro- and cleaved caspase-3 ( D ). ( E ) The cultures were stained with antibodies against BrdU ( green ) and NeuN ( red ); Hoechst 33258 ( blue ) served as counterstaining. White arrows denote mature neurons with BrdU incorporation. Scale bar = 10 μm. Mean ± SEM from N = 3. Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. *, #, and + all denote p < 0.05.
    Figure Legend Snippet: PPV-6 downregulates expression of the cell cycle/apoptosis markers as well as BrdU incorporation induced by Aβ1-42. ( A – D ) Primary cortical cultures were exposed to Aβ1-42 (5 μM), PPV-6 (250 μg/mL), or both for 48 h before detection of cyclin D1 ( A ), PCNA ( B ), p-Histone H3 ( C ), as well as pro- and cleaved caspase-3 ( D ). ( E ) The cultures were stained with antibodies against BrdU ( green ) and NeuN ( red ); Hoechst 33258 ( blue ) served as counterstaining. White arrows denote mature neurons with BrdU incorporation. Scale bar = 10 μm. Mean ± SEM from N = 3. Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. *, #, and + all denote p < 0.05.

    Techniques Used: Expressing, BrdU Incorporation Assay, Staining

    PPV-6 post-treatment downregulates expression of the cell cycle/apoptosis markers induced by Aβ25-35. ( A – D ) Primary cortical cultures were exposed to Aβ25-35 (10 μM) for 2 h; this was followed by treatment with PPV-6 (250 μg/mL) for additional 22 h in the absence of Aβ25-35 before detection of cyclin D1 ( A ), PCNA ( B ), p-Histone H3 ( C ), as well as both pro- and cleaved caspase-3 ( D ) through western blotting. ( E – G ) Similarly treated cortical cultures were subjected to immunostaining with antibodies against various cell cycle markers ( green ), including cyclin D1 ( E ), PCNA ( F ), and p-Histone H3 ( G ). The MAP-2 antibody ( red ) labeled the mature neurons; Hoechst 33258 ( blue ) served as counterstaining. White arrows denote the mature neurons positively stained with cell cycle markers. Scale bar = 10 μm. Mean ± SEM from N = 4 in ( A – D ) and N = 6 in ( E – G ). Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. * and # denote p < 0.05.
    Figure Legend Snippet: PPV-6 post-treatment downregulates expression of the cell cycle/apoptosis markers induced by Aβ25-35. ( A – D ) Primary cortical cultures were exposed to Aβ25-35 (10 μM) for 2 h; this was followed by treatment with PPV-6 (250 μg/mL) for additional 22 h in the absence of Aβ25-35 before detection of cyclin D1 ( A ), PCNA ( B ), p-Histone H3 ( C ), as well as both pro- and cleaved caspase-3 ( D ) through western blotting. ( E – G ) Similarly treated cortical cultures were subjected to immunostaining with antibodies against various cell cycle markers ( green ), including cyclin D1 ( E ), PCNA ( F ), and p-Histone H3 ( G ). The MAP-2 antibody ( red ) labeled the mature neurons; Hoechst 33258 ( blue ) served as counterstaining. White arrows denote the mature neurons positively stained with cell cycle markers. Scale bar = 10 μm. Mean ± SEM from N = 4 in ( A – D ) and N = 6 in ( E – G ). Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. * and # denote p < 0.05.

    Techniques Used: Expressing, Western Blot, Immunostaining, Labeling, Staining

    PPV-6 suppresses Aβ-induced SHH expression and blocks SHH-N-mediated neuronal CCR. ( A ) Primary cortical cultures were exposed to Aβ25-35 (10 μM) with or without PPV-6 (250 μg/mL) for 24 h before detection of SHH by means of western blotting. ( B – D ) Primary cortical cultures were exposed to SHH-N (300 ng/mL) with or without PPV-6 (250 μg/mL) for 24 h before detection of various cell cycle markers, including cyclin D1 ( B ), PCNA ( C ), and p-Histone H3 ( D ). Mean ± SEM from N = 4 in ( A ), N = 3 in ( B ), N = 3 in ( C ), and N = 5 in ( D ). Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. *, #, and + all denote p < 0.05.
    Figure Legend Snippet: PPV-6 suppresses Aβ-induced SHH expression and blocks SHH-N-mediated neuronal CCR. ( A ) Primary cortical cultures were exposed to Aβ25-35 (10 μM) with or without PPV-6 (250 μg/mL) for 24 h before detection of SHH by means of western blotting. ( B – D ) Primary cortical cultures were exposed to SHH-N (300 ng/mL) with or without PPV-6 (250 μg/mL) for 24 h before detection of various cell cycle markers, including cyclin D1 ( B ), PCNA ( C ), and p-Histone H3 ( D ). Mean ± SEM from N = 4 in ( A ), N = 3 in ( B ), N = 3 in ( C ), and N = 5 in ( D ). Data were analyzed by means of a one-way ANOVA followed by a post-hoc Tukey test. *, #, and + all denote p < 0.05.

    Techniques Used: Expressing, Western Blot

    antibodies against cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc antibodies against cyclin d1
    A Cell cycle analysis showing the distribution of H661 and PC-9 cells in different cell cycle phases post-treatment with Syrosingopine, UK-5099, or their combination ( n = 3). B Western blot detection of the level of <t>cyclin</t> <t>D1</t> protein after treatment with Syrosingopine, UK-5099, or their combination ( n = 3). C The effect of Syrosingopine and UK-5099 on the invasion capabilities of H661 and PC-9 cells was determined by Transwell invasion assays ( n = 3). D The effect of Syrosingopine and UK-5099 on the migratory capabilities of H661 and PC-9 cells was determined by a scratch wound assay ( n = 3). E Flow cytometric analysis of apoptosis via Annexin V-FITC/PI staining in H661 and PC-9 cells following exposure to Syrosingopine, UK-5099, or their combination ( n = 3). ( P -values: not significant (ns) p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).
    Antibodies Against Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Syrosingopine and UK5099 synergistically suppress non-small cell lung cancer by activating the integrated stress response"

    Article Title: Syrosingopine and UK5099 synergistically suppress non-small cell lung cancer by activating the integrated stress response

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-024-06821-4

    A Cell cycle analysis showing the distribution of H661 and PC-9 cells in different cell cycle phases post-treatment with Syrosingopine, UK-5099, or their combination ( n = 3). B Western blot detection of the level of cyclin D1 protein after treatment with Syrosingopine, UK-5099, or their combination ( n = 3). C The effect of Syrosingopine and UK-5099 on the invasion capabilities of H661 and PC-9 cells was determined by Transwell invasion assays ( n = 3). D The effect of Syrosingopine and UK-5099 on the migratory capabilities of H661 and PC-9 cells was determined by a scratch wound assay ( n = 3). E Flow cytometric analysis of apoptosis via Annexin V-FITC/PI staining in H661 and PC-9 cells following exposure to Syrosingopine, UK-5099, or their combination ( n = 3). ( P -values: not significant (ns) p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).
    Figure Legend Snippet: A Cell cycle analysis showing the distribution of H661 and PC-9 cells in different cell cycle phases post-treatment with Syrosingopine, UK-5099, or their combination ( n = 3). B Western blot detection of the level of cyclin D1 protein after treatment with Syrosingopine, UK-5099, or their combination ( n = 3). C The effect of Syrosingopine and UK-5099 on the invasion capabilities of H661 and PC-9 cells was determined by Transwell invasion assays ( n = 3). D The effect of Syrosingopine and UK-5099 on the migratory capabilities of H661 and PC-9 cells was determined by a scratch wound assay ( n = 3). E Flow cytometric analysis of apoptosis via Annexin V-FITC/PI staining in H661 and PC-9 cells following exposure to Syrosingopine, UK-5099, or their combination ( n = 3). ( P -values: not significant (ns) p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).

    Techniques Used: Cell Cycle Assay, Western Blot, Scratch Wound Assay Assay, Staining


    Structured Review

    Proteintech antibodies against cyclin d1
    Antibodies Against Cyclin D1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images


    Structured Review

    Santa Cruz Biotechnology antibody against cyclin d1
    Antibody Against Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against cyclin d1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    antibodies against cyclin d1  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
    Bioz Manufacturer Symbol Danaher Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Danaher Inc antibodies against cyclin d1
    Effect of GSK-3 inhibition by CHIR99021 on lung proliferation. ( a ) Representative images of western blot using lung cell suspensions at 12 h and 24 h after LPS-induced ALI and CHIR99021 treatment at 30 min post-LPS injection and 30 h and 72 h after LPS-induced ALI and CHIR99021 treatment at 18 h post-LPS injection blotted with <t>anti-Cyclin</t> <t>D1</t> and anti-β-actin (loading control). ( b ) Quantification of Cyclin D1 protein levels normalised to β-actin at 12 h, 24 h, 30 h, and 72 h. ( c ) Representative immunofluorescence images of PCNA, PDPN, and proSP-C expression at 24 h and 72 h. 400× magnification. Scale bars: 20 μm. ( d ) Relative quantification of total PCNA positive cells per total DAPI positive cells, total double-positive PCNA/PDPN cells per total positive PDPN area, total double-positive PCNA/proSP-C cells per total proSP-C area, and total triple-positive PCNA/proSP-C/PDPN cells per total DAPI positive cells, at 24 h and 72 h. (n = 5–11 per group. N = 2 independent experiments. Data were presented from one of them, both with statistical differences). Data are present as mean ± SEM. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, parametric and nonparametric t -test. a.u.: Arbitrary units, PCNA: Proliferating cell nuclear antigen, PDPN: Podoplanin, proSP-C: Prosurfactant protein C.
    Antibodies Against Cyclin D1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Alveolar Epithelial Cell Proliferation and Lung Regeneration in the Lipopolysaccharide-Induced Acute Lung Injury Mouse Model"

    Article Title: Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Alveolar Epithelial Cell Proliferation and Lung Regeneration in the Lipopolysaccharide-Induced Acute Lung Injury Mouse Model

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25021279

    Effect of GSK-3 inhibition by CHIR99021 on lung proliferation. ( a ) Representative images of western blot using lung cell suspensions at 12 h and 24 h after LPS-induced ALI and CHIR99021 treatment at 30 min post-LPS injection and 30 h and 72 h after LPS-induced ALI and CHIR99021 treatment at 18 h post-LPS injection blotted with anti-Cyclin D1 and anti-β-actin (loading control). ( b ) Quantification of Cyclin D1 protein levels normalised to β-actin at 12 h, 24 h, 30 h, and 72 h. ( c ) Representative immunofluorescence images of PCNA, PDPN, and proSP-C expression at 24 h and 72 h. 400× magnification. Scale bars: 20 μm. ( d ) Relative quantification of total PCNA positive cells per total DAPI positive cells, total double-positive PCNA/PDPN cells per total positive PDPN area, total double-positive PCNA/proSP-C cells per total proSP-C area, and total triple-positive PCNA/proSP-C/PDPN cells per total DAPI positive cells, at 24 h and 72 h. (n = 5–11 per group. N = 2 independent experiments. Data were presented from one of them, both with statistical differences). Data are present as mean ± SEM. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, parametric and nonparametric t -test. a.u.: Arbitrary units, PCNA: Proliferating cell nuclear antigen, PDPN: Podoplanin, proSP-C: Prosurfactant protein C.
    Figure Legend Snippet: Effect of GSK-3 inhibition by CHIR99021 on lung proliferation. ( a ) Representative images of western blot using lung cell suspensions at 12 h and 24 h after LPS-induced ALI and CHIR99021 treatment at 30 min post-LPS injection and 30 h and 72 h after LPS-induced ALI and CHIR99021 treatment at 18 h post-LPS injection blotted with anti-Cyclin D1 and anti-β-actin (loading control). ( b ) Quantification of Cyclin D1 protein levels normalised to β-actin at 12 h, 24 h, 30 h, and 72 h. ( c ) Representative immunofluorescence images of PCNA, PDPN, and proSP-C expression at 24 h and 72 h. 400× magnification. Scale bars: 20 μm. ( d ) Relative quantification of total PCNA positive cells per total DAPI positive cells, total double-positive PCNA/PDPN cells per total positive PDPN area, total double-positive PCNA/proSP-C cells per total proSP-C area, and total triple-positive PCNA/proSP-C/PDPN cells per total DAPI positive cells, at 24 h and 72 h. (n = 5–11 per group. N = 2 independent experiments. Data were presented from one of them, both with statistical differences). Data are present as mean ± SEM. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, parametric and nonparametric t -test. a.u.: Arbitrary units, PCNA: Proliferating cell nuclear antigen, PDPN: Podoplanin, proSP-C: Prosurfactant protein C.

    Techniques Used: Inhibition, Western Blot, Injection, Immunofluorescence, Expressing

    primary antibodies against cyclin d1  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
    Bioz Manufacturer Symbol Danaher Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Danaher Inc primary antibodies against cyclin d1
    Morroniside stimulates the level of cell cycle proteins. (A–D) Representative image of Western blotting as well as quantitative analysis of <t>cyclin</t> <t>D1,</t> CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vitro ( n = 4). In addition, data are denoted to be mean ± SEM. # p < 0.05 in relative to the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 in relative to OGD group. (E–H) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vivo ( n = 4). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 VS AMI group.
    Primary Antibodies Against Cyclin D1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against cyclin d1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Morroniside induces cardiomyocyte cell cycle activity and promotes cardiac repair after myocardial infarction in adult rats"

    Article Title: Morroniside induces cardiomyocyte cell cycle activity and promotes cardiac repair after myocardial infarction in adult rats

    Journal: Frontiers in Pharmacology

    doi: 10.3389/fphar.2023.1260674

    Morroniside stimulates the level of cell cycle proteins. (A–D) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vitro ( n = 4). In addition, data are denoted to be mean ± SEM. # p < 0.05 in relative to the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 in relative to OGD group. (E–H) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vivo ( n = 4). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 VS AMI group.
    Figure Legend Snippet: Morroniside stimulates the level of cell cycle proteins. (A–D) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vitro ( n = 4). In addition, data are denoted to be mean ± SEM. # p < 0.05 in relative to the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 in relative to OGD group. (E–H) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vivo ( n = 4). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 VS AMI group.

    Techniques Used: Western Blot, In Vitro, In Vivo

    antibodies against cyclin d1  (Cell Signaling Technology Inc)


    Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Cell Signaling Technology Inc antibodies against cyclin d1
    Antibodies Against Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images


    Structured Review

    Santa Cruz Biotechnology antibodies against cyclin d1
    Antibodies Against Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    antibodies against cyclin d1  (Danaher Inc)


    Bioz Verified Symbol Danaher Inc is a verified supplier
    Bioz Manufacturer Symbol Danaher Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Danaher Inc antibodies against cyclin d1
    Regeneration of intra‐splenic transplanted thyroid tissue. A) PAS staining of a whole‐spleen section showing the regenerated thyroid tissue (ST) in the spleen 16 weeks post‐transplantation. The black dashed curves (‐ – ‐) outline the ST tissue. B) H&E staining analysis of the intra‐splenic regenerated thyroid tissue at weeks 2, 4, 8, 12, and 16. Upper panels show the thyroid tissue (ST) within the spleen, while lower panels provide detailed views of the regenerated thyroid follicles. The black dashed curves (‐ – ‐) outline the ST tissue. C) Weekly monitoring of the number of thyroid follicular epithelial cells in the intra‐splenic thyroid tissue for 16 consecutive weeks, with adult thyroid as control. The value for week 0 represents the calculated number of cells in the transplanted tissue blocks. n = 5 biological replicates. D) Weekly assessment of the number of thyroid follicles in the intrasplenic thyroid tissue for 16 weeks, with adult thyroid as control. The value for week 0 represents the calculated number of follicles in the transplanted tissue blocks. n = 3 biological replicates. E) Measurement of the volume of thyroid follicles, with adult thyroid as control. n = 40. F) Determination of the height of thyroid follicular epithelial cells, with adult thyroid as control. n = 50. G) Calculation of the thyroid follicular activation index, with adult thyroid as control. n = 50. H) Evaluation of proliferative activity through PCNA immunofluorescence staining (co‐staining with GFP to identify thyroid tissue in GFP‐transgenic mice). I) Comparison of IGF‐1 levels in spleen, muscle, and subcutis. n = 5 biological replicates. J) The proliferative activity of thyroid tissue blocks after co‐incubation with spleen homogenate (SH) was determined by EdU staining. K) Western blotting analysis of the <t>Cyclin</t> <t>D1</t> expression in thyroid tissues after co‐incubation with spleen homogenate (SH), IGF‐1, and the inhibitor of the IGF‐1 receptor (picropodophyllin, AXL1717). L) Immunofluorescence staining of Cyclin D1 expression in the intra‐splenic regenerated thyroid tissue at weeks 2 and 16. Data presented as means ± SEM. Statistical analyses were performed using one‐way ANOVA followed by Tukey's multiple comparisons tests for (I). PAS, periodic acid‐Schiff; ST, intra‐splenic thyroid; SH, spleen homogenate; EdU, 5‐ethynyl‐2′‐deoxyuridine; ANOVA, analysis of variance.
    Antibodies Against Cyclin D1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars

    Images

    1) Product Images from "Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice"

    Article Title: Regeneration of Thyroid Glands in the Spleen Restores Homeostasis in Thyroidectomy Mice

    Journal: Advanced Science

    doi: 10.1002/advs.202305913

    Regeneration of intra‐splenic transplanted thyroid tissue. A) PAS staining of a whole‐spleen section showing the regenerated thyroid tissue (ST) in the spleen 16 weeks post‐transplantation. The black dashed curves (‐ – ‐) outline the ST tissue. B) H&E staining analysis of the intra‐splenic regenerated thyroid tissue at weeks 2, 4, 8, 12, and 16. Upper panels show the thyroid tissue (ST) within the spleen, while lower panels provide detailed views of the regenerated thyroid follicles. The black dashed curves (‐ – ‐) outline the ST tissue. C) Weekly monitoring of the number of thyroid follicular epithelial cells in the intra‐splenic thyroid tissue for 16 consecutive weeks, with adult thyroid as control. The value for week 0 represents the calculated number of cells in the transplanted tissue blocks. n = 5 biological replicates. D) Weekly assessment of the number of thyroid follicles in the intrasplenic thyroid tissue for 16 weeks, with adult thyroid as control. The value for week 0 represents the calculated number of follicles in the transplanted tissue blocks. n = 3 biological replicates. E) Measurement of the volume of thyroid follicles, with adult thyroid as control. n = 40. F) Determination of the height of thyroid follicular epithelial cells, with adult thyroid as control. n = 50. G) Calculation of the thyroid follicular activation index, with adult thyroid as control. n = 50. H) Evaluation of proliferative activity through PCNA immunofluorescence staining (co‐staining with GFP to identify thyroid tissue in GFP‐transgenic mice). I) Comparison of IGF‐1 levels in spleen, muscle, and subcutis. n = 5 biological replicates. J) The proliferative activity of thyroid tissue blocks after co‐incubation with spleen homogenate (SH) was determined by EdU staining. K) Western blotting analysis of the Cyclin D1 expression in thyroid tissues after co‐incubation with spleen homogenate (SH), IGF‐1, and the inhibitor of the IGF‐1 receptor (picropodophyllin, AXL1717). L) Immunofluorescence staining of Cyclin D1 expression in the intra‐splenic regenerated thyroid tissue at weeks 2 and 16. Data presented as means ± SEM. Statistical analyses were performed using one‐way ANOVA followed by Tukey's multiple comparisons tests for (I). PAS, periodic acid‐Schiff; ST, intra‐splenic thyroid; SH, spleen homogenate; EdU, 5‐ethynyl‐2′‐deoxyuridine; ANOVA, analysis of variance.
    Figure Legend Snippet: Regeneration of intra‐splenic transplanted thyroid tissue. A) PAS staining of a whole‐spleen section showing the regenerated thyroid tissue (ST) in the spleen 16 weeks post‐transplantation. The black dashed curves (‐ – ‐) outline the ST tissue. B) H&E staining analysis of the intra‐splenic regenerated thyroid tissue at weeks 2, 4, 8, 12, and 16. Upper panels show the thyroid tissue (ST) within the spleen, while lower panels provide detailed views of the regenerated thyroid follicles. The black dashed curves (‐ – ‐) outline the ST tissue. C) Weekly monitoring of the number of thyroid follicular epithelial cells in the intra‐splenic thyroid tissue for 16 consecutive weeks, with adult thyroid as control. The value for week 0 represents the calculated number of cells in the transplanted tissue blocks. n = 5 biological replicates. D) Weekly assessment of the number of thyroid follicles in the intrasplenic thyroid tissue for 16 weeks, with adult thyroid as control. The value for week 0 represents the calculated number of follicles in the transplanted tissue blocks. n = 3 biological replicates. E) Measurement of the volume of thyroid follicles, with adult thyroid as control. n = 40. F) Determination of the height of thyroid follicular epithelial cells, with adult thyroid as control. n = 50. G) Calculation of the thyroid follicular activation index, with adult thyroid as control. n = 50. H) Evaluation of proliferative activity through PCNA immunofluorescence staining (co‐staining with GFP to identify thyroid tissue in GFP‐transgenic mice). I) Comparison of IGF‐1 levels in spleen, muscle, and subcutis. n = 5 biological replicates. J) The proliferative activity of thyroid tissue blocks after co‐incubation with spleen homogenate (SH) was determined by EdU staining. K) Western blotting analysis of the Cyclin D1 expression in thyroid tissues after co‐incubation with spleen homogenate (SH), IGF‐1, and the inhibitor of the IGF‐1 receptor (picropodophyllin, AXL1717). L) Immunofluorescence staining of Cyclin D1 expression in the intra‐splenic regenerated thyroid tissue at weeks 2 and 16. Data presented as means ± SEM. Statistical analyses were performed using one‐way ANOVA followed by Tukey's multiple comparisons tests for (I). PAS, periodic acid‐Schiff; ST, intra‐splenic thyroid; SH, spleen homogenate; EdU, 5‐ethynyl‐2′‐deoxyuridine; ANOVA, analysis of variance.

    Techniques Used: Staining, Transplantation Assay, Activation Assay, Activity Assay, Immunofluorescence, Transgenic Assay, Comparison, Incubation, Western Blot, Expressing

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Danaher Inc rabbit antibodies against cyclin d1
    Rabbit Antibodies Against Cyclin D1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit antibodies against cyclin d1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc antibodies against cyclin d1
    A Cell cycle analysis showing the distribution of H661 and PC-9 cells in different cell cycle phases post-treatment with Syrosingopine, UK-5099, or their combination ( n = 3). B Western blot detection of the level of <t>cyclin</t> <t>D1</t> protein after treatment with Syrosingopine, UK-5099, or their combination ( n = 3). C The effect of Syrosingopine and UK-5099 on the invasion capabilities of H661 and PC-9 cells was determined by Transwell invasion assays ( n = 3). D The effect of Syrosingopine and UK-5099 on the migratory capabilities of H661 and PC-9 cells was determined by a scratch wound assay ( n = 3). E Flow cytometric analysis of apoptosis via Annexin V-FITC/PI staining in H661 and PC-9 cells following exposure to Syrosingopine, UK-5099, or their combination ( n = 3). ( P -values: not significant (ns) p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).
    Antibodies Against Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Proteintech antibodies against cyclin d1
    A Cell cycle analysis showing the distribution of H661 and PC-9 cells in different cell cycle phases post-treatment with Syrosingopine, UK-5099, or their combination ( n = 3). B Western blot detection of the level of <t>cyclin</t> <t>D1</t> protein after treatment with Syrosingopine, UK-5099, or their combination ( n = 3). C The effect of Syrosingopine and UK-5099 on the invasion capabilities of H661 and PC-9 cells was determined by Transwell invasion assays ( n = 3). D The effect of Syrosingopine and UK-5099 on the migratory capabilities of H661 and PC-9 cells was determined by a scratch wound assay ( n = 3). E Flow cytometric analysis of apoptosis via Annexin V-FITC/PI staining in H661 and PC-9 cells following exposure to Syrosingopine, UK-5099, or their combination ( n = 3). ( P -values: not significant (ns) p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).
    Antibodies Against Cyclin D1, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology antibody against cyclin d1
    A Cell cycle analysis showing the distribution of H661 and PC-9 cells in different cell cycle phases post-treatment with Syrosingopine, UK-5099, or their combination ( n = 3). B Western blot detection of the level of <t>cyclin</t> <t>D1</t> protein after treatment with Syrosingopine, UK-5099, or their combination ( n = 3). C The effect of Syrosingopine and UK-5099 on the invasion capabilities of H661 and PC-9 cells was determined by Transwell invasion assays ( n = 3). D The effect of Syrosingopine and UK-5099 on the migratory capabilities of H661 and PC-9 cells was determined by a scratch wound assay ( n = 3). E Flow cytometric analysis of apoptosis via Annexin V-FITC/PI staining in H661 and PC-9 cells following exposure to Syrosingopine, UK-5099, or their combination ( n = 3). ( P -values: not significant (ns) p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).
    Antibody Against Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against cyclin d1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Danaher Inc antibodies against cyclin d1
    Effect of GSK-3 inhibition by CHIR99021 on lung proliferation. ( a ) Representative images of western blot using lung cell suspensions at 12 h and 24 h after LPS-induced ALI and CHIR99021 treatment at 30 min post-LPS injection and 30 h and 72 h after LPS-induced ALI and CHIR99021 treatment at 18 h post-LPS injection blotted with <t>anti-Cyclin</t> <t>D1</t> and anti-β-actin (loading control). ( b ) Quantification of Cyclin D1 protein levels normalised to β-actin at 12 h, 24 h, 30 h, and 72 h. ( c ) Representative immunofluorescence images of PCNA, PDPN, and proSP-C expression at 24 h and 72 h. 400× magnification. Scale bars: 20 μm. ( d ) Relative quantification of total PCNA positive cells per total DAPI positive cells, total double-positive PCNA/PDPN cells per total positive PDPN area, total double-positive PCNA/proSP-C cells per total proSP-C area, and total triple-positive PCNA/proSP-C/PDPN cells per total DAPI positive cells, at 24 h and 72 h. (n = 5–11 per group. N = 2 independent experiments. Data were presented from one of them, both with statistical differences). Data are present as mean ± SEM. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, parametric and nonparametric t -test. a.u.: Arbitrary units, PCNA: Proliferating cell nuclear antigen, PDPN: Podoplanin, proSP-C: Prosurfactant protein C.
    Antibodies Against Cyclin D1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Danaher Inc primary antibodies against cyclin d1
    Morroniside stimulates the level of cell cycle proteins. (A–D) Representative image of Western blotting as well as quantitative analysis of <t>cyclin</t> <t>D1,</t> CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vitro ( n = 4). In addition, data are denoted to be mean ± SEM. # p < 0.05 in relative to the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 in relative to OGD group. (E–H) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vivo ( n = 4). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 VS AMI group.
    Primary Antibodies Against Cyclin D1, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies against cyclin d1/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    primary antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    86
    Santa Cruz Biotechnology antibodies against cyclin d1
    Morroniside stimulates the level of cell cycle proteins. (A–D) Representative image of Western blotting as well as quantitative analysis of <t>cyclin</t> <t>D1,</t> CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vitro ( n = 4). In addition, data are denoted to be mean ± SEM. # p < 0.05 in relative to the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 in relative to OGD group. (E–H) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vivo ( n = 4). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 VS AMI group.
    Antibodies Against Cyclin D1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cyclin d1/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against cyclin d1 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    A Cell cycle analysis showing the distribution of H661 and PC-9 cells in different cell cycle phases post-treatment with Syrosingopine, UK-5099, or their combination ( n = 3). B Western blot detection of the level of cyclin D1 protein after treatment with Syrosingopine, UK-5099, or their combination ( n = 3). C The effect of Syrosingopine and UK-5099 on the invasion capabilities of H661 and PC-9 cells was determined by Transwell invasion assays ( n = 3). D The effect of Syrosingopine and UK-5099 on the migratory capabilities of H661 and PC-9 cells was determined by a scratch wound assay ( n = 3). E Flow cytometric analysis of apoptosis via Annexin V-FITC/PI staining in H661 and PC-9 cells following exposure to Syrosingopine, UK-5099, or their combination ( n = 3). ( P -values: not significant (ns) p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).

    Journal: Cell Death & Disease

    Article Title: Syrosingopine and UK5099 synergistically suppress non-small cell lung cancer by activating the integrated stress response

    doi: 10.1038/s41419-024-06821-4

    Figure Lengend Snippet: A Cell cycle analysis showing the distribution of H661 and PC-9 cells in different cell cycle phases post-treatment with Syrosingopine, UK-5099, or their combination ( n = 3). B Western blot detection of the level of cyclin D1 protein after treatment with Syrosingopine, UK-5099, or their combination ( n = 3). C The effect of Syrosingopine and UK-5099 on the invasion capabilities of H661 and PC-9 cells was determined by Transwell invasion assays ( n = 3). D The effect of Syrosingopine and UK-5099 on the migratory capabilities of H661 and PC-9 cells was determined by a scratch wound assay ( n = 3). E Flow cytometric analysis of apoptosis via Annexin V-FITC/PI staining in H661 and PC-9 cells following exposure to Syrosingopine, UK-5099, or their combination ( n = 3). ( P -values: not significant (ns) p > 0.05, * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001).

    Article Snippet: Antibodies against Cyclin D1 (E3P5S), p-eIF2α (Ser51) (D9G8), eIF2α (D7D3), β-Actin (8H10D10), and Vinculin (E1E9V) were purchased from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Cell Cycle Assay, Western Blot, Scratch Wound Assay Assay, Staining

    Effect of GSK-3 inhibition by CHIR99021 on lung proliferation. ( a ) Representative images of western blot using lung cell suspensions at 12 h and 24 h after LPS-induced ALI and CHIR99021 treatment at 30 min post-LPS injection and 30 h and 72 h after LPS-induced ALI and CHIR99021 treatment at 18 h post-LPS injection blotted with anti-Cyclin D1 and anti-β-actin (loading control). ( b ) Quantification of Cyclin D1 protein levels normalised to β-actin at 12 h, 24 h, 30 h, and 72 h. ( c ) Representative immunofluorescence images of PCNA, PDPN, and proSP-C expression at 24 h and 72 h. 400× magnification. Scale bars: 20 μm. ( d ) Relative quantification of total PCNA positive cells per total DAPI positive cells, total double-positive PCNA/PDPN cells per total positive PDPN area, total double-positive PCNA/proSP-C cells per total proSP-C area, and total triple-positive PCNA/proSP-C/PDPN cells per total DAPI positive cells, at 24 h and 72 h. (n = 5–11 per group. N = 2 independent experiments. Data were presented from one of them, both with statistical differences). Data are present as mean ± SEM. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, parametric and nonparametric t -test. a.u.: Arbitrary units, PCNA: Proliferating cell nuclear antigen, PDPN: Podoplanin, proSP-C: Prosurfactant protein C.

    Journal: International Journal of Molecular Sciences

    Article Title: Glycogen Synthase Kinase-3 Inhibition by CHIR99021 Promotes Alveolar Epithelial Cell Proliferation and Lung Regeneration in the Lipopolysaccharide-Induced Acute Lung Injury Mouse Model

    doi: 10.3390/ijms25021279

    Figure Lengend Snippet: Effect of GSK-3 inhibition by CHIR99021 on lung proliferation. ( a ) Representative images of western blot using lung cell suspensions at 12 h and 24 h after LPS-induced ALI and CHIR99021 treatment at 30 min post-LPS injection and 30 h and 72 h after LPS-induced ALI and CHIR99021 treatment at 18 h post-LPS injection blotted with anti-Cyclin D1 and anti-β-actin (loading control). ( b ) Quantification of Cyclin D1 protein levels normalised to β-actin at 12 h, 24 h, 30 h, and 72 h. ( c ) Representative immunofluorescence images of PCNA, PDPN, and proSP-C expression at 24 h and 72 h. 400× magnification. Scale bars: 20 μm. ( d ) Relative quantification of total PCNA positive cells per total DAPI positive cells, total double-positive PCNA/PDPN cells per total positive PDPN area, total double-positive PCNA/proSP-C cells per total proSP-C area, and total triple-positive PCNA/proSP-C/PDPN cells per total DAPI positive cells, at 24 h and 72 h. (n = 5–11 per group. N = 2 independent experiments. Data were presented from one of them, both with statistical differences). Data are present as mean ± SEM. * p -value < 0.05, ** p -value < 0.01, *** p -value < 0.001, parametric and nonparametric t -test. a.u.: Arbitrary units, PCNA: Proliferating cell nuclear antigen, PDPN: Podoplanin, proSP-C: Prosurfactant protein C.

    Article Snippet: Following this, membranes were incubated ON at 4 °C with primary antibodies against cyclin D1 (Abcam (Cambridge, UK), ab16663, 1/200), cleaved caspase-3 (Cell Signaling Technology, #9661, 1/250), HOPX (Sigma, Millipore, HPA030180, 1/1000), proSP-C (Chemicon (Tokyo, Japan), AB3786, 1/1000), PDPN (R&D Systems, AF3244, 1/750), AQP5 (Abcam, ab78486, 1/2000), and β-actin (Abcam, ab8224, 1/10000), diluted in 5% Milk or 5% BSA according to the manufacturer’s instructions.

    Techniques: Inhibition, Western Blot, Injection, Immunofluorescence, Expressing

    Morroniside stimulates the level of cell cycle proteins. (A–D) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vitro ( n = 4). In addition, data are denoted to be mean ± SEM. # p < 0.05 in relative to the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 in relative to OGD group. (E–H) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vivo ( n = 4). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 VS AMI group.

    Journal: Frontiers in Pharmacology

    Article Title: Morroniside induces cardiomyocyte cell cycle activity and promotes cardiac repair after myocardial infarction in adult rats

    doi: 10.3389/fphar.2023.1260674

    Figure Lengend Snippet: Morroniside stimulates the level of cell cycle proteins. (A–D) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vitro ( n = 4). In addition, data are denoted to be mean ± SEM. # p < 0.05 in relative to the control group; * p < 0.05, ** p < 0.01, *** p < 0.001 in relative to OGD group. (E–H) Representative image of Western blotting as well as quantitative analysis of cyclin D1, CDK4, cyclin A2 and cyclin B1 denoted to be a fraction of GAPDH in vivo ( n = 4). Data are presented as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 VS AMI group.

    Article Snippet: Primary antibodies against cyclin D1 (1:1,000, ab134175; Abcam), cyclin-dependent kinase 4 (CDK4) (1:1,000, 09173-4F11; Sigma-Aldrich), cyclin A2 (1:1,000, ab181591; Abcam), and cyclin B1 (1:1,000, 4135S; Cell Signaling Technology, Beverly, MA, United States) were used for immunodetection.

    Techniques: Western Blot, In Vitro, In Vivo