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antibodies against cx3cr1 ab8020  (Danaher Inc)


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    Danaher Inc antibodies against cx3cr1 ab8020
    ( A ) UMAP visualization of leukocytes in aortic root and ascending aortas from 20-week-old WT and Fbn1 C1041G/+ male mice. ( B ) Selected marker gene expression in distinct leukocyte subtypes. ( C ) Cell counts in each leukocyte subtype. WT, n = 6,508 cells versus Fbn1 C1041G/+ , n = 3,827 cells. χ 2 test to compare the cell quantitative changes. ( D ) GO analysis of genes enriched in <t>CX3CR1</t> + macrophages (cluster 0). ( E ) The expression of CX3CR1 in distinct leukocyte subtypes. ( F ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from WT and Fbn1 C1041G/+ mice at different ages. n = 5. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) En face immunofluorescence staining of VE-cadherin (green) and CX3CR1 (red) in the intima of ascending aortas from 6- or 20-week-old WT and Fbn1 C1041G/+ mice. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the percentages of CX3CR1 + cells (red cells) in total intima cells (the number of nuclei) averaged from 4 randomly selected areas of ascending aortas for each mouse. n = 4 mice. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( H ) Immunofluorescence staining of CX3CR1 (green) and CD31 (red) in cross-sections of normal aortic tissues from control individuals (controls) and aneurysmal tissues from MFS patients. The nuclei were stained blue with DAPI. Scale bars:100 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in CD31 intimal areas averaged from 4 randomly selected areas for each patient. The average of intensities of CX3CR1 staining in normal controls was set as 1, while the intensities in MFS samples were presented as the relative values. n = 4. * P < 0.05 by unpaired Student’s t test.
    Antibodies Against Cx3cr1 Ab8020, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cx3cr1 ab8020/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    antibodies against cx3cr1 ab8020 - by Bioz Stars, 2025-03
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    1) Product Images from "Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice"

    Article Title: Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice

    Journal: The Journal of Clinical Investigation

    doi: 10.1172/JCI178198

    ( A ) UMAP visualization of leukocytes in aortic root and ascending aortas from 20-week-old WT and Fbn1 C1041G/+ male mice. ( B ) Selected marker gene expression in distinct leukocyte subtypes. ( C ) Cell counts in each leukocyte subtype. WT, n = 6,508 cells versus Fbn1 C1041G/+ , n = 3,827 cells. χ 2 test to compare the cell quantitative changes. ( D ) GO analysis of genes enriched in CX3CR1 + macrophages (cluster 0). ( E ) The expression of CX3CR1 in distinct leukocyte subtypes. ( F ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from WT and Fbn1 C1041G/+ mice at different ages. n = 5. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) En face immunofluorescence staining of VE-cadherin (green) and CX3CR1 (red) in the intima of ascending aortas from 6- or 20-week-old WT and Fbn1 C1041G/+ mice. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the percentages of CX3CR1 + cells (red cells) in total intima cells (the number of nuclei) averaged from 4 randomly selected areas of ascending aortas for each mouse. n = 4 mice. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( H ) Immunofluorescence staining of CX3CR1 (green) and CD31 (red) in cross-sections of normal aortic tissues from control individuals (controls) and aneurysmal tissues from MFS patients. The nuclei were stained blue with DAPI. Scale bars:100 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in CD31 intimal areas averaged from 4 randomly selected areas for each patient. The average of intensities of CX3CR1 staining in normal controls was set as 1, while the intensities in MFS samples were presented as the relative values. n = 4. * P < 0.05 by unpaired Student’s t test.
    Figure Legend Snippet: ( A ) UMAP visualization of leukocytes in aortic root and ascending aortas from 20-week-old WT and Fbn1 C1041G/+ male mice. ( B ) Selected marker gene expression in distinct leukocyte subtypes. ( C ) Cell counts in each leukocyte subtype. WT, n = 6,508 cells versus Fbn1 C1041G/+ , n = 3,827 cells. χ 2 test to compare the cell quantitative changes. ( D ) GO analysis of genes enriched in CX3CR1 + macrophages (cluster 0). ( E ) The expression of CX3CR1 in distinct leukocyte subtypes. ( F ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from WT and Fbn1 C1041G/+ mice at different ages. n = 5. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) En face immunofluorescence staining of VE-cadherin (green) and CX3CR1 (red) in the intima of ascending aortas from 6- or 20-week-old WT and Fbn1 C1041G/+ mice. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the percentages of CX3CR1 + cells (red cells) in total intima cells (the number of nuclei) averaged from 4 randomly selected areas of ascending aortas for each mouse. n = 4 mice. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( H ) Immunofluorescence staining of CX3CR1 (green) and CD31 (red) in cross-sections of normal aortic tissues from control individuals (controls) and aneurysmal tissues from MFS patients. The nuclei were stained blue with DAPI. Scale bars:100 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in CD31 intimal areas averaged from 4 randomly selected areas for each patient. The average of intensities of CX3CR1 staining in normal controls was set as 1, while the intensities in MFS samples were presented as the relative values. n = 4. * P < 0.05 by unpaired Student’s t test.

    Techniques Used: Marker, Expressing, Flow Cytometry, Comparison, Immunofluorescence, Staining, Control, Fluorescence

    ( A ) Experimental workflow of eliminating intimal CX3CR1 + macrophages to observe TAA development in Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice. ( B ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. The average quantity of CX3CR1 + macrophages in the vehicle group was set as 100%, while that in the DT group was relative to that in the vehicle group. n = 9 for vehicle and n = 8 for DT. * P < 0.05 by unpaired Student’s t test. ( C ) En face immunofluorescence staining of intimal CX3CR1 + macrophages (red) and VE-cadherin (green) in ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in intima averaged from 4 randomly selected areas for each mouse. The average of intensities of CX3CR1 staining in vehicle group were set as 1, while the intensities in DT group were presented as the relative values. n = 4 mice. * P < 0.05 by unpaired Student’s t test. ( D ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas in Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice at different ages with or without DT-mediated depletion. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( E ) Quantification of aortic root and ascending aorta diameters measured by transthoracic echocardiography. n = 10 for vehicle and n = 8 for DT. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( F ) EVG staining of the aortic roots in 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. n = 8 for each group. * P < 0.05 by Mann-Whitney U test for elastin degradation grade and unpaired Student’s t test for aortic thickness.
    Figure Legend Snippet: ( A ) Experimental workflow of eliminating intimal CX3CR1 + macrophages to observe TAA development in Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice. ( B ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. The average quantity of CX3CR1 + macrophages in the vehicle group was set as 100%, while that in the DT group was relative to that in the vehicle group. n = 9 for vehicle and n = 8 for DT. * P < 0.05 by unpaired Student’s t test. ( C ) En face immunofluorescence staining of intimal CX3CR1 + macrophages (red) and VE-cadherin (green) in ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in intima averaged from 4 randomly selected areas for each mouse. The average of intensities of CX3CR1 staining in vehicle group were set as 1, while the intensities in DT group were presented as the relative values. n = 4 mice. * P < 0.05 by unpaired Student’s t test. ( D ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas in Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice at different ages with or without DT-mediated depletion. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( E ) Quantification of aortic root and ascending aorta diameters measured by transthoracic echocardiography. n = 10 for vehicle and n = 8 for DT. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( F ) EVG staining of the aortic roots in 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. n = 8 for each group. * P < 0.05 by Mann-Whitney U test for elastin degradation grade and unpaired Student’s t test for aortic thickness.

    Techniques Used: Flow Cytometry, Immunofluorescence, Staining, Fluorescence, MANN-WHITNEY

    ( A ) The pseudotime path of the VSMC transcriptome derived from the scRNA-Seq data on WT and Fbn1 C1041G/+ mice. ( B ) KEGG pathways enriched using the top 100 genes upregulated in Fbn1 C1041G/+ VSMCs compared with WT cells. ( C ) Bioinformatic analysis of ligand-receptor interactions between CX3CR1 + macrophages and VSMCs based on scRNA-Seq data. ( D ) The workflow of CX3CR1 + and CX3CR1 – macrophages isolated from MFS patients by FACS. ( E ) ELISA measurements of predicted ligand proteins secreted from CX3CR1 + or CX3CR1 – macrophages in conditioned media. n = 6. * P < 0.05 by unpaired Student’s t test.
    Figure Legend Snippet: ( A ) The pseudotime path of the VSMC transcriptome derived from the scRNA-Seq data on WT and Fbn1 C1041G/+ mice. ( B ) KEGG pathways enriched using the top 100 genes upregulated in Fbn1 C1041G/+ VSMCs compared with WT cells. ( C ) Bioinformatic analysis of ligand-receptor interactions between CX3CR1 + macrophages and VSMCs based on scRNA-Seq data. ( D ) The workflow of CX3CR1 + and CX3CR1 – macrophages isolated from MFS patients by FACS. ( E ) ELISA measurements of predicted ligand proteins secreted from CX3CR1 + or CX3CR1 – macrophages in conditioned media. n = 6. * P < 0.05 by unpaired Student’s t test.

    Techniques Used: Derivative Assay, Isolation, Enzyme-linked Immunosorbent Assay

    ( A ) The experimental workflow of the evaluation of gene expression in MFS patient-specific iPSC-derived VSMCs with 24-hour treatment of conditioned media produced from 48-hour cultures of MFS patient-derived CX3CR1 + and CX3CR1 – macrophages. ( B ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditioned media treatment. n = 4. * P < 0.05 by unpaired Student’s t test. ( C and D ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditional media treatment in the presence of adalimumab (10 μg/mL) or IgG. n = 4. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( E and F ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditioned media treatment in the presence of teprotumumab (200 μg/mL) or IgG. n = 4. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) Real-time PCR of gene expression in the aortic root and ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. n = 4. * P < 0.05 by unpaired Student’s t test.
    Figure Legend Snippet: ( A ) The experimental workflow of the evaluation of gene expression in MFS patient-specific iPSC-derived VSMCs with 24-hour treatment of conditioned media produced from 48-hour cultures of MFS patient-derived CX3CR1 + and CX3CR1 – macrophages. ( B ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditioned media treatment. n = 4. * P < 0.05 by unpaired Student’s t test. ( C and D ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditional media treatment in the presence of adalimumab (10 μg/mL) or IgG. n = 4. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( E and F ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditioned media treatment in the presence of teprotumumab (200 μg/mL) or IgG. n = 4. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) Real-time PCR of gene expression in the aortic root and ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. n = 4. * P < 0.05 by unpaired Student’s t test.

    Techniques Used: Expressing, Derivative Assay, Produced, Real-time Polymerase Chain Reaction, Comparison

    ( A ) The experimental workflow of parabiosis of age-matched Fbn1 C1041G/+ mice and Cx3cr1 GFP/+ Fbn1 C1041G/+ mice. ( B ) Flow cytometry analysis of GFP-expressing CX3CR1 + macrophages in aortic root and ascending aortas from Fbn1 C1041G/+ and Cx3cr1 GFP/+ Fbn1 C1041G/+ parabionts. n = 5. Unpaired Student’s t test. ( C ) En face immunofluorescence staining of GFP-expressed intimal CX3CR1 + macrophages in ascending aortas from Fbn1 C1041G/+ and Cx3cr1 GFP/+ Fbn1 C1041G/+ parabionts. Scale bars: 20 μm. ( D ) The experimental workflow of bone marrow transplantation (BMT) with Fbn1 C1041G/+ bone marrow cells infected with lentivirus encoding shRNA targeting TNF-α or IGF1 or control shRNA in 12-week-old Fbn1 C1041G/+ mice. ( E ) Real-time PCR of gene expression in aortic root and ascending aortas from Fbn1 C1041G/+ mice after BMT. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( F ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas in Fbn1 C1041G/+ mice at different ages with control, Igf1, or Tnfα shRNA treatment. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( G ) Quantification of aortic root and ascending aorta diameters measured by transthoracic echocardiography. n = 5. * ,# P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( H ) EVG staining of aortic roots in 24-week-old mice with control, Igf1, or Tnfα shRNA treatment. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison.
    Figure Legend Snippet: ( A ) The experimental workflow of parabiosis of age-matched Fbn1 C1041G/+ mice and Cx3cr1 GFP/+ Fbn1 C1041G/+ mice. ( B ) Flow cytometry analysis of GFP-expressing CX3CR1 + macrophages in aortic root and ascending aortas from Fbn1 C1041G/+ and Cx3cr1 GFP/+ Fbn1 C1041G/+ parabionts. n = 5. Unpaired Student’s t test. ( C ) En face immunofluorescence staining of GFP-expressed intimal CX3CR1 + macrophages in ascending aortas from Fbn1 C1041G/+ and Cx3cr1 GFP/+ Fbn1 C1041G/+ parabionts. Scale bars: 20 μm. ( D ) The experimental workflow of bone marrow transplantation (BMT) with Fbn1 C1041G/+ bone marrow cells infected with lentivirus encoding shRNA targeting TNF-α or IGF1 or control shRNA in 12-week-old Fbn1 C1041G/+ mice. ( E ) Real-time PCR of gene expression in aortic root and ascending aortas from Fbn1 C1041G/+ mice after BMT. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( F ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas in Fbn1 C1041G/+ mice at different ages with control, Igf1, or Tnfα shRNA treatment. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( G ) Quantification of aortic root and ascending aorta diameters measured by transthoracic echocardiography. n = 5. * ,# P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( H ) EVG staining of aortic roots in 24-week-old mice with control, Igf1, or Tnfα shRNA treatment. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison.

    Techniques Used: Flow Cytometry, Expressing, Immunofluorescence, Staining, Transplantation Assay, Infection, shRNA, Control, Real-time Polymerase Chain Reaction, Comparison

    ( A – D ) Six-week-old Fbn1 C1041G/+ mice were intraperitoneally injected with RS504393 (4 mg/kg/d) until age 26 weeks. Age-matched WT mice were used as a normal control. ( A ) Flow cytometry analysis of Ly6C + monocytes and CX3CR1 + macrophages in aortic root and ascending aortas. n = 4. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( B ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( C ) Quantification of transthoracic echocardiography. n = 4 for WT and n = 8 for Fbn1 C1041G/+ mice. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( D ) EVG staining of the aortic roots. n = 5. * P < 0.05 by Kruskal-Wallis test followed by Dunn’s test for elastin degradation grade and 1-way ANOVA followed by Tukey’s test for post hoc comparison for aortic thickness. ( E – H ) Twenty-four-week-old Fbn1 C1041G/+ mice were intraperitoneally injected with RS504393 (4 mg/kg/d) until age 36 weeks. Age-matched WT mice were used as a normal control. ( E ) Flow cytometry analysis of Ly6C + monocytes and CX3CR1 + macrophages in aortic root and ascending aortas. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( F ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( G ) Quantification of transthoracic echocardiography. n = 5. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( H ) EVG staining of aortic roots. n = 5. * P < 0.05 by Kruskal-Wallis test followed by Dunn’s test for elastin degradation grade and 1-way ANOVA followed by Tukey’s test for post hoc comparison for aortic thickness.
    Figure Legend Snippet: ( A – D ) Six-week-old Fbn1 C1041G/+ mice were intraperitoneally injected with RS504393 (4 mg/kg/d) until age 26 weeks. Age-matched WT mice were used as a normal control. ( A ) Flow cytometry analysis of Ly6C + monocytes and CX3CR1 + macrophages in aortic root and ascending aortas. n = 4. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( B ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( C ) Quantification of transthoracic echocardiography. n = 4 for WT and n = 8 for Fbn1 C1041G/+ mice. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( D ) EVG staining of the aortic roots. n = 5. * P < 0.05 by Kruskal-Wallis test followed by Dunn’s test for elastin degradation grade and 1-way ANOVA followed by Tukey’s test for post hoc comparison for aortic thickness. ( E – H ) Twenty-four-week-old Fbn1 C1041G/+ mice were intraperitoneally injected with RS504393 (4 mg/kg/d) until age 36 weeks. Age-matched WT mice were used as a normal control. ( E ) Flow cytometry analysis of Ly6C + monocytes and CX3CR1 + macrophages in aortic root and ascending aortas. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( F ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( G ) Quantification of transthoracic echocardiography. n = 5. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( H ) EVG staining of aortic roots. n = 5. * P < 0.05 by Kruskal-Wallis test followed by Dunn’s test for elastin degradation grade and 1-way ANOVA followed by Tukey’s test for post hoc comparison for aortic thickness.

    Techniques Used: Injection, Control, Flow Cytometry, Comparison, Staining

    CX3CR1 + macrophages mainly located in the aortic intima and originated from circulating Ly6C + monocytes. Intimal CX3CR1 + macrophages upregulated inflammatory genes, including IL6 , MCP1 , and CXCL2 , in VSMCs by producing and secreting TNF-α and IGF1, thereby aggravating TAA development in MFS. Either elimination of intimal CX3CR1 + macrophages or administration of a CCR2 inhibitor to suppress monocyte recruitment efficiently alleviated TAA progression in MFS.
    Figure Legend Snippet: CX3CR1 + macrophages mainly located in the aortic intima and originated from circulating Ly6C + monocytes. Intimal CX3CR1 + macrophages upregulated inflammatory genes, including IL6 , MCP1 , and CXCL2 , in VSMCs by producing and secreting TNF-α and IGF1, thereby aggravating TAA development in MFS. Either elimination of intimal CX3CR1 + macrophages or administration of a CCR2 inhibitor to suppress monocyte recruitment efficiently alleviated TAA progression in MFS.

    Techniques Used:



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    antibodies against cx3cr1 ab8020  (Danaher Inc)
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    Danaher Inc antibodies against cx3cr1 ab8020
    ( A ) UMAP visualization of leukocytes in aortic root and ascending aortas from 20-week-old WT and Fbn1 C1041G/+ male mice. ( B ) Selected marker gene expression in distinct leukocyte subtypes. ( C ) Cell counts in each leukocyte subtype. WT, n = 6,508 cells versus Fbn1 C1041G/+ , n = 3,827 cells. χ 2 test to compare the cell quantitative changes. ( D ) GO analysis of genes enriched in <t>CX3CR1</t> + macrophages (cluster 0). ( E ) The expression of CX3CR1 in distinct leukocyte subtypes. ( F ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from WT and Fbn1 C1041G/+ mice at different ages. n = 5. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) En face immunofluorescence staining of VE-cadherin (green) and CX3CR1 (red) in the intima of ascending aortas from 6- or 20-week-old WT and Fbn1 C1041G/+ mice. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the percentages of CX3CR1 + cells (red cells) in total intima cells (the number of nuclei) averaged from 4 randomly selected areas of ascending aortas for each mouse. n = 4 mice. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( H ) Immunofluorescence staining of CX3CR1 (green) and CD31 (red) in cross-sections of normal aortic tissues from control individuals (controls) and aneurysmal tissues from MFS patients. The nuclei were stained blue with DAPI. Scale bars:100 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in CD31 intimal areas averaged from 4 randomly selected areas for each patient. The average of intensities of CX3CR1 staining in normal controls was set as 1, while the intensities in MFS samples were presented as the relative values. n = 4. * P < 0.05 by unpaired Student’s t test.
    Antibodies Against Cx3cr1 Ab8020, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against cx3cr1 ab8020/product/Danaher Inc
    Average 86 stars, based on 1 article reviews
    antibodies against cx3cr1 ab8020 - by Bioz Stars, 2025-03
    86/100 stars
    		  Buy from Supplier

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    ( A ) UMAP visualization of leukocytes in aortic root and ascending aortas from 20-week-old WT and Fbn1 C1041G/+ male mice. ( B ) Selected marker gene expression in distinct leukocyte subtypes. ( C ) Cell counts in each leukocyte subtype. WT, n = 6,508 cells versus Fbn1 C1041G/+ , n = 3,827 cells. χ 2 test to compare the cell quantitative changes. ( D ) GO analysis of genes enriched in CX3CR1 + macrophages (cluster 0). ( E ) The expression of CX3CR1 in distinct leukocyte subtypes. ( F ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from WT and Fbn1 C1041G/+ mice at different ages. n = 5. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) En face immunofluorescence staining of VE-cadherin (green) and CX3CR1 (red) in the intima of ascending aortas from 6- or 20-week-old WT and Fbn1 C1041G/+ mice. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the percentages of CX3CR1 + cells (red cells) in total intima cells (the number of nuclei) averaged from 4 randomly selected areas of ascending aortas for each mouse. n = 4 mice. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( H ) Immunofluorescence staining of CX3CR1 (green) and CD31 (red) in cross-sections of normal aortic tissues from control individuals (controls) and aneurysmal tissues from MFS patients. The nuclei were stained blue with DAPI. Scale bars:100 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in CD31 intimal areas averaged from 4 randomly selected areas for each patient. The average of intensities of CX3CR1 staining in normal controls was set as 1, while the intensities in MFS samples were presented as the relative values. n = 4. * P < 0.05 by unpaired Student’s t test.

    Journal: The Journal of Clinical Investigation

    Article Title: Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice

    doi: 10.1172/JCI178198

    Figure Lengend Snippet: ( A ) UMAP visualization of leukocytes in aortic root and ascending aortas from 20-week-old WT and Fbn1 C1041G/+ male mice. ( B ) Selected marker gene expression in distinct leukocyte subtypes. ( C ) Cell counts in each leukocyte subtype. WT, n = 6,508 cells versus Fbn1 C1041G/+ , n = 3,827 cells. χ 2 test to compare the cell quantitative changes. ( D ) GO analysis of genes enriched in CX3CR1 + macrophages (cluster 0). ( E ) The expression of CX3CR1 in distinct leukocyte subtypes. ( F ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from WT and Fbn1 C1041G/+ mice at different ages. n = 5. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) En face immunofluorescence staining of VE-cadherin (green) and CX3CR1 (red) in the intima of ascending aortas from 6- or 20-week-old WT and Fbn1 C1041G/+ mice. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the percentages of CX3CR1 + cells (red cells) in total intima cells (the number of nuclei) averaged from 4 randomly selected areas of ascending aortas for each mouse. n = 4 mice. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( H ) Immunofluorescence staining of CX3CR1 (green) and CD31 (red) in cross-sections of normal aortic tissues from control individuals (controls) and aneurysmal tissues from MFS patients. The nuclei were stained blue with DAPI. Scale bars:100 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in CD31 intimal areas averaged from 4 randomly selected areas for each patient. The average of intensities of CX3CR1 staining in normal controls was set as 1, while the intensities in MFS samples were presented as the relative values. n = 4. * P < 0.05 by unpaired Student’s t test.

    Article Snippet: Antibodies against CX3CR1 (ab8020) and CD68 (ab955) were obtained from Abcam.

    Techniques: Marker, Expressing, Flow Cytometry, Comparison, Immunofluorescence, Staining, Control, Fluorescence

    ( A ) Experimental workflow of eliminating intimal CX3CR1 + macrophages to observe TAA development in Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice. ( B ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. The average quantity of CX3CR1 + macrophages in the vehicle group was set as 100%, while that in the DT group was relative to that in the vehicle group. n = 9 for vehicle and n = 8 for DT. * P < 0.05 by unpaired Student’s t test. ( C ) En face immunofluorescence staining of intimal CX3CR1 + macrophages (red) and VE-cadherin (green) in ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in intima averaged from 4 randomly selected areas for each mouse. The average of intensities of CX3CR1 staining in vehicle group were set as 1, while the intensities in DT group were presented as the relative values. n = 4 mice. * P < 0.05 by unpaired Student’s t test. ( D ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas in Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice at different ages with or without DT-mediated depletion. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( E ) Quantification of aortic root and ascending aorta diameters measured by transthoracic echocardiography. n = 10 for vehicle and n = 8 for DT. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( F ) EVG staining of the aortic roots in 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. n = 8 for each group. * P < 0.05 by Mann-Whitney U test for elastin degradation grade and unpaired Student’s t test for aortic thickness.

    Journal: The Journal of Clinical Investigation

    Article Title: Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice

    doi: 10.1172/JCI178198

    Figure Lengend Snippet: ( A ) Experimental workflow of eliminating intimal CX3CR1 + macrophages to observe TAA development in Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice. ( B ) Flow cytometry analysis of CX3CR1 + macrophages in aortic root and ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. The average quantity of CX3CR1 + macrophages in the vehicle group was set as 100%, while that in the DT group was relative to that in the vehicle group. n = 9 for vehicle and n = 8 for DT. * P < 0.05 by unpaired Student’s t test. ( C ) En face immunofluorescence staining of intimal CX3CR1 + macrophages (red) and VE-cadherin (green) in ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. The nuclei were stained blue with DAPI. Scale bars: 20 μm. Data were quantified as the relative fluorescence intensities of CX3CR1 staining in intima averaged from 4 randomly selected areas for each mouse. The average of intensities of CX3CR1 staining in vehicle group were set as 1, while the intensities in DT group were presented as the relative values. n = 4 mice. * P < 0.05 by unpaired Student’s t test. ( D ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas in Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice at different ages with or without DT-mediated depletion. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( E ) Quantification of aortic root and ascending aorta diameters measured by transthoracic echocardiography. n = 10 for vehicle and n = 8 for DT. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( F ) EVG staining of the aortic roots in 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. n = 8 for each group. * P < 0.05 by Mann-Whitney U test for elastin degradation grade and unpaired Student’s t test for aortic thickness.

    Article Snippet: Antibodies against CX3CR1 (ab8020) and CD68 (ab955) were obtained from Abcam.

    Techniques: Flow Cytometry, Immunofluorescence, Staining, Fluorescence, MANN-WHITNEY

    ( A ) The pseudotime path of the VSMC transcriptome derived from the scRNA-Seq data on WT and Fbn1 C1041G/+ mice. ( B ) KEGG pathways enriched using the top 100 genes upregulated in Fbn1 C1041G/+ VSMCs compared with WT cells. ( C ) Bioinformatic analysis of ligand-receptor interactions between CX3CR1 + macrophages and VSMCs based on scRNA-Seq data. ( D ) The workflow of CX3CR1 + and CX3CR1 – macrophages isolated from MFS patients by FACS. ( E ) ELISA measurements of predicted ligand proteins secreted from CX3CR1 + or CX3CR1 – macrophages in conditioned media. n = 6. * P < 0.05 by unpaired Student’s t test.

    Journal: The Journal of Clinical Investigation

    Article Title: Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice

    doi: 10.1172/JCI178198

    Figure Lengend Snippet: ( A ) The pseudotime path of the VSMC transcriptome derived from the scRNA-Seq data on WT and Fbn1 C1041G/+ mice. ( B ) KEGG pathways enriched using the top 100 genes upregulated in Fbn1 C1041G/+ VSMCs compared with WT cells. ( C ) Bioinformatic analysis of ligand-receptor interactions between CX3CR1 + macrophages and VSMCs based on scRNA-Seq data. ( D ) The workflow of CX3CR1 + and CX3CR1 – macrophages isolated from MFS patients by FACS. ( E ) ELISA measurements of predicted ligand proteins secreted from CX3CR1 + or CX3CR1 – macrophages in conditioned media. n = 6. * P < 0.05 by unpaired Student’s t test.

    Article Snippet: Antibodies against CX3CR1 (ab8020) and CD68 (ab955) were obtained from Abcam.

    Techniques: Derivative Assay, Isolation, Enzyme-linked Immunosorbent Assay

    ( A ) The experimental workflow of the evaluation of gene expression in MFS patient-specific iPSC-derived VSMCs with 24-hour treatment of conditioned media produced from 48-hour cultures of MFS patient-derived CX3CR1 + and CX3CR1 – macrophages. ( B ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditioned media treatment. n = 4. * P < 0.05 by unpaired Student’s t test. ( C and D ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditional media treatment in the presence of adalimumab (10 μg/mL) or IgG. n = 4. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( E and F ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditioned media treatment in the presence of teprotumumab (200 μg/mL) or IgG. n = 4. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) Real-time PCR of gene expression in the aortic root and ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. n = 4. * P < 0.05 by unpaired Student’s t test.

    Journal: The Journal of Clinical Investigation

    Article Title: Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice

    doi: 10.1172/JCI178198

    Figure Lengend Snippet: ( A ) The experimental workflow of the evaluation of gene expression in MFS patient-specific iPSC-derived VSMCs with 24-hour treatment of conditioned media produced from 48-hour cultures of MFS patient-derived CX3CR1 + and CX3CR1 – macrophages. ( B ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditioned media treatment. n = 4. * P < 0.05 by unpaired Student’s t test. ( C and D ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditional media treatment in the presence of adalimumab (10 μg/mL) or IgG. n = 4. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( E and F ) Real-time PCR of gene expression in MFS patient-specific iPSC-derived VSMCs with conditioned media treatment in the presence of teprotumumab (200 μg/mL) or IgG. n = 4. * P < 0.05 by 2-way ANOVA followed by Tukey’s test for post hoc comparison. ( G ) Real-time PCR of gene expression in the aortic root and ascending aortas from 21-week-old Cx3cr1 -CreER T2 iDTR F/+ Fbn1 C1041G/+ mice with or without DT-mediated depletion. n = 4. * P < 0.05 by unpaired Student’s t test.

    Article Snippet: Antibodies against CX3CR1 (ab8020) and CD68 (ab955) were obtained from Abcam.

    Techniques: Expressing, Derivative Assay, Produced, Real-time Polymerase Chain Reaction, Comparison

    ( A ) The experimental workflow of parabiosis of age-matched Fbn1 C1041G/+ mice and Cx3cr1 GFP/+ Fbn1 C1041G/+ mice. ( B ) Flow cytometry analysis of GFP-expressing CX3CR1 + macrophages in aortic root and ascending aortas from Fbn1 C1041G/+ and Cx3cr1 GFP/+ Fbn1 C1041G/+ parabionts. n = 5. Unpaired Student’s t test. ( C ) En face immunofluorescence staining of GFP-expressed intimal CX3CR1 + macrophages in ascending aortas from Fbn1 C1041G/+ and Cx3cr1 GFP/+ Fbn1 C1041G/+ parabionts. Scale bars: 20 μm. ( D ) The experimental workflow of bone marrow transplantation (BMT) with Fbn1 C1041G/+ bone marrow cells infected with lentivirus encoding shRNA targeting TNF-α or IGF1 or control shRNA in 12-week-old Fbn1 C1041G/+ mice. ( E ) Real-time PCR of gene expression in aortic root and ascending aortas from Fbn1 C1041G/+ mice after BMT. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( F ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas in Fbn1 C1041G/+ mice at different ages with control, Igf1, or Tnfα shRNA treatment. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( G ) Quantification of aortic root and ascending aorta diameters measured by transthoracic echocardiography. n = 5. * ,# P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( H ) EVG staining of aortic roots in 24-week-old mice with control, Igf1, or Tnfα shRNA treatment. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison.

    Journal: The Journal of Clinical Investigation

    Article Title: Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice

    doi: 10.1172/JCI178198

    Figure Lengend Snippet: ( A ) The experimental workflow of parabiosis of age-matched Fbn1 C1041G/+ mice and Cx3cr1 GFP/+ Fbn1 C1041G/+ mice. ( B ) Flow cytometry analysis of GFP-expressing CX3CR1 + macrophages in aortic root and ascending aortas from Fbn1 C1041G/+ and Cx3cr1 GFP/+ Fbn1 C1041G/+ parabionts. n = 5. Unpaired Student’s t test. ( C ) En face immunofluorescence staining of GFP-expressed intimal CX3CR1 + macrophages in ascending aortas from Fbn1 C1041G/+ and Cx3cr1 GFP/+ Fbn1 C1041G/+ parabionts. Scale bars: 20 μm. ( D ) The experimental workflow of bone marrow transplantation (BMT) with Fbn1 C1041G/+ bone marrow cells infected with lentivirus encoding shRNA targeting TNF-α or IGF1 or control shRNA in 12-week-old Fbn1 C1041G/+ mice. ( E ) Real-time PCR of gene expression in aortic root and ascending aortas from Fbn1 C1041G/+ mice after BMT. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( F ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas in Fbn1 C1041G/+ mice at different ages with control, Igf1, or Tnfα shRNA treatment. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( G ) Quantification of aortic root and ascending aorta diameters measured by transthoracic echocardiography. n = 5. * ,# P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( H ) EVG staining of aortic roots in 24-week-old mice with control, Igf1, or Tnfα shRNA treatment. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison.

    Article Snippet: Antibodies against CX3CR1 (ab8020) and CD68 (ab955) were obtained from Abcam.

    Techniques: Flow Cytometry, Expressing, Immunofluorescence, Staining, Transplantation Assay, Infection, shRNA, Control, Real-time Polymerase Chain Reaction, Comparison

    ( A – D ) Six-week-old Fbn1 C1041G/+ mice were intraperitoneally injected with RS504393 (4 mg/kg/d) until age 26 weeks. Age-matched WT mice were used as a normal control. ( A ) Flow cytometry analysis of Ly6C + monocytes and CX3CR1 + macrophages in aortic root and ascending aortas. n = 4. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( B ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( C ) Quantification of transthoracic echocardiography. n = 4 for WT and n = 8 for Fbn1 C1041G/+ mice. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( D ) EVG staining of the aortic roots. n = 5. * P < 0.05 by Kruskal-Wallis test followed by Dunn’s test for elastin degradation grade and 1-way ANOVA followed by Tukey’s test for post hoc comparison for aortic thickness. ( E – H ) Twenty-four-week-old Fbn1 C1041G/+ mice were intraperitoneally injected with RS504393 (4 mg/kg/d) until age 36 weeks. Age-matched WT mice were used as a normal control. ( E ) Flow cytometry analysis of Ly6C + monocytes and CX3CR1 + macrophages in aortic root and ascending aortas. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( F ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( G ) Quantification of transthoracic echocardiography. n = 5. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( H ) EVG staining of aortic roots. n = 5. * P < 0.05 by Kruskal-Wallis test followed by Dunn’s test for elastin degradation grade and 1-way ANOVA followed by Tukey’s test for post hoc comparison for aortic thickness.

    Journal: The Journal of Clinical Investigation

    Article Title: Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice

    doi: 10.1172/JCI178198

    Figure Lengend Snippet: ( A – D ) Six-week-old Fbn1 C1041G/+ mice were intraperitoneally injected with RS504393 (4 mg/kg/d) until age 26 weeks. Age-matched WT mice were used as a normal control. ( A ) Flow cytometry analysis of Ly6C + monocytes and CX3CR1 + macrophages in aortic root and ascending aortas. n = 4. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( B ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( C ) Quantification of transthoracic echocardiography. n = 4 for WT and n = 8 for Fbn1 C1041G/+ mice. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( D ) EVG staining of the aortic roots. n = 5. * P < 0.05 by Kruskal-Wallis test followed by Dunn’s test for elastin degradation grade and 1-way ANOVA followed by Tukey’s test for post hoc comparison for aortic thickness. ( E – H ) Twenty-four-week-old Fbn1 C1041G/+ mice were intraperitoneally injected with RS504393 (4 mg/kg/d) until age 36 weeks. Age-matched WT mice were used as a normal control. ( E ) Flow cytometry analysis of Ly6C + monocytes and CX3CR1 + macrophages in aortic root and ascending aortas. n = 5. * P < 0.05 by 1-way ANOVA followed by Tukey’s test for post hoc comparison. ( F ) Representative transthoracic echocardiographic images of the aortic root and ascending aortas. Scale bars: 2 mm. White arrows depict the sinus of Valsalva measurements. Yellow arrows depict ascending aorta measurement. ( G ) Quantification of transthoracic echocardiography. n = 5. * P < 0.05 by repeated-measures ANOVA with the Greenhouse-Geisser adjustment followed by Bonferroni’s post hoc comparisons. ( H ) EVG staining of aortic roots. n = 5. * P < 0.05 by Kruskal-Wallis test followed by Dunn’s test for elastin degradation grade and 1-way ANOVA followed by Tukey’s test for post hoc comparison for aortic thickness.

    Article Snippet: Antibodies against CX3CR1 (ab8020) and CD68 (ab955) were obtained from Abcam.

    Techniques: Injection, Control, Flow Cytometry, Comparison, Staining

    CX3CR1 + macrophages mainly located in the aortic intima and originated from circulating Ly6C + monocytes. Intimal CX3CR1 + macrophages upregulated inflammatory genes, including IL6 , MCP1 , and CXCL2 , in VSMCs by producing and secreting TNF-α and IGF1, thereby aggravating TAA development in MFS. Either elimination of intimal CX3CR1 + macrophages or administration of a CCR2 inhibitor to suppress monocyte recruitment efficiently alleviated TAA progression in MFS.

    Journal: The Journal of Clinical Investigation

    Article Title: Inhibition of aortic CX3CR1 + macrophages mitigates thoracic aortic aneurysm progression in Marfan syndrome in mice

    doi: 10.1172/JCI178198

    Figure Lengend Snippet: CX3CR1 + macrophages mainly located in the aortic intima and originated from circulating Ly6C + monocytes. Intimal CX3CR1 + macrophages upregulated inflammatory genes, including IL6 , MCP1 , and CXCL2 , in VSMCs by producing and secreting TNF-α and IGF1, thereby aggravating TAA development in MFS. Either elimination of intimal CX3CR1 + macrophages or administration of a CCR2 inhibitor to suppress monocyte recruitment efficiently alleviated TAA progression in MFS.

    Article Snippet: Antibodies against CX3CR1 (ab8020) and CD68 (ab955) were obtained from Abcam.

    Techniques: