antibodies against cdk12  (Danaher Inc)

Journal: Cell Death & Disease

Article Title: CRISPR screening identifies CDK12 as a conservative vulnerability of prostate cancer

doi: 10.1038/s41419-021-04027-6

Figure Lengend Snippet: A Schematic illustration of CRISPR/Cas9 screening to identify conserved kinases in C4–2 cells cultured with normal or 10–25 μM enzalutamide. B RRA scores for the kinome. The top 20 candidate genes are marked in red. C Heatmap showing the correlation of the kinome among the normal and AR antagonism groups on the 21st and 28th days. D Venn diagram showing the overlap of the top 20 candidate genes from each group. E Typical images of immunostaining of the CDK12 protein in PCa and normal prostate tissues. The scale bars represent 100 µm. F IHC score of the CDK12 protein levels in PCa and normal prostate tissues. G Level of CDK12 knockout in PCa cells, as measured by western blotting. H PCa cell proliferation assessed by the colony formation assay. I PCa cell viability assessed by the CCK-8 cell viability assay.

Article Snippet: Antibodies against CDK12 (ab37914) were purchased from Abcam.

Techniques: CRISPR, Cell Culture, Immunostaining, Knock-Out, Western Blot, Colony Assay, CCK-8 Assay, Viability Assay

Journal: Cell Death & Disease

Article Title: CRISPR screening identifies CDK12 as a conservative vulnerability of prostate cancer

doi: 10.1038/s41419-021-04027-6

Figure Lengend Snippet: A The numbers of differentially expressed genes (DEGs) induced upon either DMSO or THZ531 treatment in LNCaP cells at 6 h. B Log2 fold changes in DEGs, total downregulated genes (TDGs) and significantly downregulated genes (SDGs) expression induced by THZ531 in LNCaP cells for 6 h. C KEGG analysis of significant DEGs in LNCaP cells treated with THZ531 for 6 h. D GSEA revealed that gene sets of androgen response and AR signature were significantly downregulated in THZ531-treated LNCaP cells. E Heatmap showing the downregulation of AR score genes in THZ531-treated LNCaP cells. F Venn diagram showing the SDGs among different doses of THZ531 as CDK12-ISTs. G Heatmap showing the downregulation of prostate basal/luminal cell genes and AR-regulated genes related to CDK12-ISTs. H Enriched GO functional categories of CDK12-ISTs. I Log2 fold changes in the mRNA abundance of CDK12-ISTs and SDGs.

Article Snippet: Antibodies against CDK12 (ab37914) were purchased from Abcam.

Techniques: Expressing, Functional Assay

Journal: Cell Death & Disease

Article Title: CRISPR screening identifies CDK12 as a conservative vulnerability of prostate cancer

doi: 10.1038/s41419-021-04027-6

Figure Lengend Snippet: A Hockey stick plots showing input-normalized, rank-ordered H3K27ac signals and SE-associated genes. B KEGG analysis of SE-associated genes. C Venn diagram showing the candidate genes between CDK12-ISTs and SE-associated genes (top). Log2 fold changes of SE-driven CDK12-ISTs compared to significantly downregulated SE-associated genes (bottom). D Kaplan–Meier curves indicating the associations between the mRNA levels of eight candidate genes and DFS in patients with PCa in TCGA database. E GRIN3A mRNA levels in various types of human cancer. F GRIN3A mRNA levels in PCa tissues and normal prostate tissues. G GRIN3A mRNA levels in remission and recurrent PCa. H GRIN3A mRNA expression in primary PCa and PCa with regional lymph node metastasis. N0: no regional lymph node metastasis; N1: metastases in 1 to 3 axillary lymph nodes. I The effectiveness of GRIN3A knockout in PCa cells was measured by western blotting. J , K PCa cell proliferation and viability were assessed by a colony formation assay and CCK-8 cell viability assay, respectively.

Article Snippet: Antibodies against CDK12 (ab37914) were purchased from Abcam.

Techniques: Expressing, Knock-Out, Western Blot, Colony Assay, CCK-8 Assay, Viability Assay