Journal: Journal of Microbiology and Biotechnology

Article Title: Bifidobacterium bifidum DS0908 and Bifidobacterium longum DS0950 Culture-Supernatants Ameliorate Obesity-Related Characteristics in Mice with High-Fat Diet-Induced Obesity

doi: 10.4014/jmb.2210.10046

Figure Lengend Snippet: ( A ) Schematic representation of C3H10T1/2 MSCs differentiation timeline. ( B ) mRNA expression levels of the major thermogenic markers Ucp1 , Pgc1α , Prdm16 and Pparγ after DS0908 and DS0950 treatment in differentiated C3H10T1/2 mesenchymal stem cells (MSCs). ( C ) Thermogenic marker UCP1, PPARγ, PGC1α and PRDM16 protein expression levels after DS0908 and DS0950 treatments in differentiated C3H10T1/2 MSCs. ( D and E ) mRNA expression levels of beige ( Cd137 , Fgf21 , P2rx5 and Tbx1 ), brown ( Cox2 ) and white ( aP2 , Psat1 , Resistin and Serpina3k ) adipocyte-specific markers after DS0908 and DS0950 treatments in differentiated C3H10T1/2 MSCs. Tbp was used as an internal control gene and β‐actin as a protein loading control. The data from three individual experiments are expressed as the average ± standard error mean (SEM). *, **, *** and ns indicate p < 0.05, < 0.01, < 0.001 and non-significant, respectively, to express the statistically significant differences between the control (MDI) and the treatment groups in the figures. The protein band intensities were measured using ImageJ. Adipogenic differentiation medium, MDI: 0.5mM IBMX, 1 μM dexamethasone and 10 μg/ml insulin; 1 μM Rosiglitazone (Rosi); DS0908 = B. bifidum DS0908; DS0950 = B. longum DS0950.

Article Snippet: Antibodies against aP2 , AMPK, p-AMPK (Thr172), p38 MAPK, p-p38 MAPK (Thr180/Tyr182), p-CREB (Ser133), p-PKA substrate and anti-rabbit IgG anti-mouse IgG antibodies conjugated with horseradish peroxidase were acquired from Cell Signaling Technology (USA).

Techniques: Expressing, Marker